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The map label for this gene is metZ [H]

Identifier: 50085409

GI number: 50085409

Start: 2278989

End: 2280179

Strand: Reverse

Name: metZ [H]

Synonym: ACIAD2314

Alternate gene names: 50085409

Gene position: 2280179-2278989 (Counterclockwise)

Preceding gene: 50085415

Following gene: 50085408

Centisome position: 63.36

GC content: 43.24

Gene sequence:

>1191_bases
ATGCATAGCCAAAACGATGAATTAGAATACCAGTTAGATACTTTAGCAATTCGTACAGGTCATACCCGTAGTTTTGAGGG
TGAACATGCTGAACCTATTTTCTTGACCTCATCTTTCGTTTATGAAAATGCGGCTGAGGCAGCGGCAAAATTTTCAGGTG
AAGTATCAGGTAATATCTATTCTCGTTTTACCAATCCAACGGTTGCCATGTTTGAAAAGCGTTTGGCTGCTATGGATGGT
GCGGAGCGTGCGGTAGCGACCAGTTCAGGCATGGGCGCTCTTTTATCGGTCTGCATGGCTTTTTTAAAAGCAGGCGATCA
TGTGATTTGCTCGCGTGCTGTATTTGGTTCAATTGTGGCCTTGTTTGAAAAATATGTTGCCAAATTTGCTGTAGATATCA
CTTTTGTTGATTTAAACGATCTGGATGCATGGAAAAATGCGATGCGCCCAGAAACCAAAATGTTGTTTATCGAATCTCCA
TCGAATCCCTTGGCTGAAATTGCGGATATTCCAGCACTTGCAGAGATTGCTCATGCCCACAATGCAATGCTTGTGGTGGA
CAACAGCTTTTGTACACCTGCATTACAGCAACCACTCAAACTTGGTGCAGACTTAGTCATGTATTCGGCGACCAAATATA
TTGATGGTCAGGGACGCGCACTTGGTGGAGCAGTTACAGGCAGTCATAAATTACTCGAAGAAGTCTTTGGTGTAGTACGT
ACACTTGGGCCATCAATGAGTCCGTTCAATGCATGGGTATTCTTAAAAGGTCTAGAAACTTTACGTTTAAGAATGAAAGC
GCATTGTGAAAATGCCCAACAGCTTGCAGAGTGGCTAGATCAGCACAAAAACGTTGAAAAAGTGTATTACGCAGGTTTAC
CACAGCATATTGGTCATGAACGCGCGACCAAACAGCAACAGGGTTTCGGCGGTATTGTCTCATTTGTGGTGAAAGGTGGA
CGCGAAGGGGCTTGGAAAGTCATCGACAATACCCAATTTATTTCAATCACAGGTAATTTGGGTGATGTGAAATCGACCAT
TACCCATCCCGCAACAACCACACATGGTAAATTGTCACCTGAAGCAAAACAGGCAGCAGGCATACAGGAAGGTTTGATTC
GGGTTTCTGTTGGTTTAGAAGATATTTCAGATATTATCCGCGACTTAGATCGTGGTTTGGCTTTAATTTAA

Upstream 100 bases:

>100_bases
AAGCGATAATTTTATCTTTTACCTTGACCTTAAATACCGTACACGCTCAAAATGTTGGCAGTTTTTTCACATGAGCGGTC
TTTAGACTGAGAACAGAGCA

Downstream 100 bases:

>100_bases
ACTCATTAAGTTAAGTTGTTTTGGAGAAAAATTGATGAACATTAATATGCTTATGCAGCAAGCACAGCGCATGCAAAAAG
ATATGGAAGCCAATGCTAAG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MHSQNDELEYQLDTLAIRTGHTRSFEGEHAEPIFLTSSFVYENAAEAAAKFSGEVSGNIYSRFTNPTVAMFEKRLAAMDG
AERAVATSSGMGALLSVCMAFLKAGDHVICSRAVFGSIVALFEKYVAKFAVDITFVDLNDLDAWKNAMRPETKMLFIESP
SNPLAEIADIPALAEIAHAHNAMLVVDNSFCTPALQQPLKLGADLVMYSATKYIDGQGRALGGAVTGSHKLLEEVFGVVR
TLGPSMSPFNAWVFLKGLETLRLRMKAHCENAQQLAEWLDQHKNVEKVYYAGLPQHIGHERATKQQQGFGGIVSFVVKGG
REGAWKVIDNTQFISITGNLGDVKSTITHPATTTHGKLSPEAKQAAGIQEGLIRVSVGLEDISDIIRDLDRGLALI

Sequences:

>Translated_396_residues
MHSQNDELEYQLDTLAIRTGHTRSFEGEHAEPIFLTSSFVYENAAEAAAKFSGEVSGNIYSRFTNPTVAMFEKRLAAMDG
AERAVATSSGMGALLSVCMAFLKAGDHVICSRAVFGSIVALFEKYVAKFAVDITFVDLNDLDAWKNAMRPETKMLFIESP
SNPLAEIADIPALAEIAHAHNAMLVVDNSFCTPALQQPLKLGADLVMYSATKYIDGQGRALGGAVTGSHKLLEEVFGVVR
TLGPSMSPFNAWVFLKGLETLRLRMKAHCENAQQLAEWLDQHKNVEKVYYAGLPQHIGHERATKQQQGFGGIVSFVVKGG
REGAWKVIDNTQFISITGNLGDVKSTITHPATTTHGKLSPEAKQAAGIQEGLIRVSVGLEDISDIIRDLDRGLALI
>Mature_396_residues
MHSQNDELEYQLDTLAIRTGHTRSFEGEHAEPIFLTSSFVYENAAEAAAKFSGEVSGNIYSRFTNPTVAMFEKRLAAMDG
AERAVATSSGMGALLSVCMAFLKAGDHVICSRAVFGSIVALFEKYVAKFAVDITFVDLNDLDAWKNAMRPETKMLFIESP
SNPLAEIADIPALAEIAHAHNAMLVVDNSFCTPALQQPLKLGADLVMYSATKYIDGQGRALGGAVTGSHKLLEEVFGVVR
TLGPSMSPFNAWVFLKGLETLRLRMKAHCENAQQLAEWLDQHKNVEKVYYAGLPQHIGHERATKQQQGFGGIVSFVVKGG
REGAWKVIDNTQFISITGNLGDVKSTITHPATTTHGKLSPEAKQAAGIQEGLIRVSVGLEDISDIIRDLDRGLALI

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=38.109756097561, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=34.4512195121951, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=32.1100917431193, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=35.9375, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789383, Length=395, Percent_Identity=26.8354430379747, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=357, Percent_Identity=36.4145658263305, Blast_Score=234, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17560056, Length=353, Percent_Identity=35.6940509915014, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=367, Percent_Identity=33.5149863760218, Blast_Score=215, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=34.1743119266055, Blast_Score=219, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6319307, Length=390, Percent_Identity=34.1025641025641, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=25.4950495049505, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=311, Percent_Identity=26.6881028938907, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=342, Percent_Identity=27.1929824561404, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922554, Length=367, Percent_Identity=37.3297002724796, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43011; Mature: 43011

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSQNDELEYQLDTLAIRTGHTRSFEGEHAEPIFLTSSFVYENAAEAAAKFSGEVSGNIY
CCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHH
SRFTNPTVAMFEKRLAAMDGAERAVATSSGMGALLSVCMAFLKAGDHVICSRAVFGSIVA
HHCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
LFEKYVAKFAVDITFVDLNDLDAWKNAMRPETKMLFIESPSNPLAEIADIPALAEIAHAH
HHHHHHHHHEEEEEEEECCCHHHHHHHCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHC
NAMLVVDNSFCTPALQQPLKLGADLVMYSATKYIDGQGRALGGAVTGSHKLLEEVFGVVR
CEEEEEECCCCCHHHHHHHHHCCHHEEHHHHHEECCCCCEECCCCCCHHHHHHHHHHHHH
TLGPSMSPFNAWVFLKGLETLRLRMKAHCENAQQLAEWLDQHKNVEKVYYAGLPQHIGHE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCHHHHCHH
RATKQQQGFGGIVSFVVKGGREGAWKVIDNTQFISITGNLGDVKSTITHPATTTHGKLSP
HHHHHHCCCHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCHHHHHHCCCCCCCCCCCCC
EAKQAAGIQEGLIRVSVGLEDISDIIRDLDRGLALI
HHHHHCCHHHCCEEEECCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHSQNDELEYQLDTLAIRTGHTRSFEGEHAEPIFLTSSFVYENAAEAAAKFSGEVSGNIY
CCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHH
SRFTNPTVAMFEKRLAAMDGAERAVATSSGMGALLSVCMAFLKAGDHVICSRAVFGSIVA
HHCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
LFEKYVAKFAVDITFVDLNDLDAWKNAMRPETKMLFIESPSNPLAEIADIPALAEIAHAH
HHHHHHHHHEEEEEEEECCCHHHHHHHCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHC
NAMLVVDNSFCTPALQQPLKLGADLVMYSATKYIDGQGRALGGAVTGSHKLLEEVFGVVR
CEEEEEECCCCCHHHHHHHHHCCHHEEHHHHHEECCCCCEECCCCCCHHHHHHHHHHHHH
TLGPSMSPFNAWVFLKGLETLRLRMKAHCENAQQLAEWLDQHKNVEKVYYAGLPQHIGHE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCHHHHCHH
RATKQQQGFGGIVSFVVKGGREGAWKVIDNTQFISITGNLGDVKSTITHPATTTHGKLSP
HHHHHHCCCHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCHHHHHHCCCCCCCCCCCCC
EAKQAAGIQEGLIRVSVGLEDISDIIRDLDRGLALI
HHHHHCCHHHCCEEEECCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]