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The map label for this gene is queF

Identifier: 50085364

GI number: 50085364

Start: 2232240

End: 2233055

Strand: Direct

Name: queF

Synonym: ACIAD2261

Alternate gene names: 50085364

Gene position: 2232240-2233055 (Clockwise)

Preceding gene: 50085363

Following gene: 50085365

Centisome position: 62.03

GC content: 38.97

Gene sequence:

>816_bases
ATGAGTGTAGAACAATCTTTATTAGGTAAAGAAACAGAATATCCAACAACTTACAGCCCAAGTGTTTTGTTTCCAATTTC
TCGCGATGCCGCTCGTGAAAAATATGCACAAATTGAAGGAATTTCGCAAGGTAAAGACTGGTGGCATGTATTCGAGCTTT
CATGGCTTAATTCAGATAACATTCCTCAAGTCGCAATTGGTCGAATCACCCTGCCAGCGACTTCGCCTTATCTGATTGAA
TCAAAATCCTTAAAGCTCTACTTTAATAGCTTAAATTTTCATGTTTTTGCATCTAAGCAAGAACTAATTGACCTAGTTGA
AAAAGATTTAAGTCAAGCAGCCAATGCAAAAGTTTCTCTTGAATTGTTTGATGTAGATTCGCTAGAAATCAGTAAACCAA
GCGGGATGTGCATTGATGATTTAAAACCTGAGCGCTTAGAATCTCACCCTGATGCCAGTTTATTAAAACTCGATGCAAAC
GAACAACATGATGCGGATGTAACATTATATTCACATTTATTGCGTAGTAATTGTCCTGTTACTGGTCAGCCTGACTGGGG
AACCGTGTTTATTCGCTATACGGGGAGAAAACATTGTTACCGCAGTATTCTGGCGTATATTATTTCTTACCGTCAGCATA
ATGGTTTTCATGAACAGTGTGTCGAACAGATTTATGCCGACATCTGGAAAAATCTACAGCCTGAAAAACTGATGGTATAT
GCAACGTACACACGTCGCGGTGGATTGGATATTAATCCATGTCGTGTATCTGATTTGTCATGGATGCCAAATCCTATTCG
GTTGGCTCGACAATAA

Upstream 100 bases:

>100_bases
ATGTAAATGTTTCATTATCACTCATCGTCGTGACTTTATGCTGTGCCATTTTATATGGAGTAGCTTATTATTTACTTTCT
CGTGGTTCAGGAATGCGTGA

Downstream 100 bases:

>100_bases
AATGAATAATTGAGGATCAAACATGCCATTTTTTGGTTTTACTCCATCTGATGCTTTACTTAGAAACATTCAAACAGGTA
TCGAGAAGAAAAACTCAAGT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSVEQSLLGKETEYPTTYSPSVLFPISRDAAREKYAQIEGISQGKDWWHVFELSWLNSDNIPQVAIGRITLPATSPYLIE
SKSLKLYFNSLNFHVFASKQELIDLVEKDLSQAANAKVSLELFDVDSLEISKPSGMCIDDLKPERLESHPDASLLKLDAN
EQHDADVTLYSHLLRSNCPVTGQPDWGTVFIRYTGRKHCYRSILAYIISYRQHNGFHEQCVEQIYADIWKNLQPEKLMVY
ATYTRRGGLDINPCRVSDLSWMPNPIRLARQ

Sequences:

>Translated_271_residues
MSVEQSLLGKETEYPTTYSPSVLFPISRDAAREKYAQIEGISQGKDWWHVFELSWLNSDNIPQVAIGRITLPATSPYLIE
SKSLKLYFNSLNFHVFASKQELIDLVEKDLSQAANAKVSLELFDVDSLEISKPSGMCIDDLKPERLESHPDASLLKLDAN
EQHDADVTLYSHLLRSNCPVTGQPDWGTVFIRYTGRKHCYRSILAYIISYRQHNGFHEQCVEQIYADIWKNLQPEKLMVY
ATYTRRGGLDINPCRVSDLSWMPNPIRLARQ
>Mature_270_residues
SVEQSLLGKETEYPTTYSPSVLFPISRDAAREKYAQIEGISQGKDWWHVFELSWLNSDNIPQVAIGRITLPATSPYLIES
KSLKLYFNSLNFHVFASKQELIDLVEKDLSQAANAKVSLELFDVDSLEISKPSGMCIDDLKPERLESHPDASLLKLDANE
QHDADVTLYSHLLRSNCPVTGQPDWGTVFIRYTGRKHCYRSILAYIISYRQHNGFHEQCVEQIYADIWKNLQPEKLMVYA
TYTRRGGLDINPCRVSDLSWMPNPIRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=269, Percent_Identity=44.6096654275093, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_ACIAD (Q6FA61)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046874.1
- ProteinModelPortal:   Q6FA61
- SMR:   Q6FA61
- STRING:   Q6FA61
- GeneID:   2880015
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2261
- NMPDR:   fig|62977.3.peg.2161
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   Q6FA61
- ProtClustDB:   PRK11792
- BioCyc:   ASP62977:ACIAD2261-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31043; Mature: 30911

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEQSLLGKETEYPTTYSPSVLFPISRDAAREKYAQIEGISQGKDWWHVFELSWLNSDN
CCCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
IPQVAIGRITLPATSPYLIESKSLKLYFNSLNFHVFASKQELIDLVEKDLSQAANAKVSL
CCEEEEEEEECCCCCCEEEECCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCCEEEE
ELFDVDSLEISKPSGMCIDDLKPERLESHPDASLLKLDANEQHDADVTLYSHLLRSNCPV
EEEECCCEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
TGQPDWGTVFIRYTGRKHCYRSILAYIISYRQHNGFHEQCVEQIYADIWKNLQPEKLMVY
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEE
ATYTRRGGLDINPCRVSDLSWMPNPIRLARQ
EEEECCCCCCCCCEEECCCCCCCCHHHHCCC
>Mature Secondary Structure 
SVEQSLLGKETEYPTTYSPSVLFPISRDAAREKYAQIEGISQGKDWWHVFELSWLNSDN
CCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
IPQVAIGRITLPATSPYLIESKSLKLYFNSLNFHVFASKQELIDLVEKDLSQAANAKVSL
CCEEEEEEEECCCCCCEEEECCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCCEEEE
ELFDVDSLEISKPSGMCIDDLKPERLESHPDASLLKLDANEQHDADVTLYSHLLRSNCPV
EEEECCCEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
TGQPDWGTVFIRYTGRKHCYRSILAYIISYRQHNGFHEQCVEQIYADIWKNLQPEKLMVY
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEE
ATYTRRGGLDINPCRVSDLSWMPNPIRLARQ
EEEECCCCCCCCCEEECCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA