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The map label for this gene is glyA

Identifier: 50085359

GI number: 50085359

Start: 2226219

End: 2227472

Strand: Reverse

Name: glyA

Synonym: ACIAD2255

Alternate gene names: 50085359

Gene position: 2227472-2226219 (Counterclockwise)

Preceding gene: 50085360

Following gene: 50085358

Centisome position: 61.9

GC content: 43.14

Gene sequence:

>1254_bases
ATGTTTGCCAACATTTCTCTTTCTGAATTTGATCCAGAATTGGCCAAATCAATCGAAGCTGAAGATGCGCGTCAGGAAGC
GCATATTGAGTTGATTGCTTCTGAAAACTATTGCTCTCCTGCGGTGATGGAAGCGCAAGGATCAAAACTTACCAATAAAT
ATGCAGAAGGTTATCCTGGCAAACGCTATTATGGCGGCTGCGAATATGTAGACGTGATTGAACAGCTTGCAATTGACCGT
GCTAAAGCGCTCTTTGGTGCTGATTATGCAAACGTTCAGCCACATGCTGGCTCACAAGCAAACTCTGCCGTATACCTTGC
ATTACTTAATGCGGGTGATACTGTTTTGGGTATGAGTCTTGCTCACGGTGGTCACTTGACTCACGGTGCAAAAGTAAACT
TCTCTGGTAAAACATACAATGCCGTTCAGTACGGTCTTAACCCTGAAACAGGCGAAATCGATTATGACGAAGTTGAGCGC
TTAGCAATAGAACACAAACCGCGTATGATCGTTGCAGGTTTCTCTGCATATAGCCGTATTGTAGATTGGCAACGTTTCCG
CGATATCGCAGATAAAGTAGGCGCTTACTTATTTGTTGATATGGCTCATGTGGCTGGTTTGGTTGCTGCCGGTGTTTATC
CAAGCCCAGTTCAAATTGCTGATGTCACCACAACCACGACCCATAAAACCCTTCGTGGTCCACGTTCTGGTTTGATTCTT
GCAAAAGCAAATGAAGAAATTGAGAAAAAACTTCAATCTGCTGTATTCCCTGGTAACCAAGGTGGTCCACTTGTACATGC
GATTGCTGCAAAAGCAGTCTGCTTTAAAGAAGCAATGGCACCAGAATATAAAGCTTATCAACAACAAGTAGTCAAAAATG
CTCAAGCCATGGCTGAAGTATTAATTGCTCGTGGTTATGATATTGTTTCTGGTGGTACAGATAACCACTTGTTCCTACTT
TCTTTGATCAAGCAAGATGTCACTGGTAAAGAAGCAGATGCATGGTTGGGTAATGCAAACATTACTGTCAACAAAAATGC
GGTTCCAAATGACCCACGCTCTCCATTTGTAACTTCTGGTATTCGTATTGGTACGCCAGCAGTAACGACTCGTGGTTTTG
GTGAAGCTGAAGTGCGCGACTTGGCTAGCTGGATTGCAGACGTTTTAGACAGCAAAGGTGATGAAAAAGTCATTGCTGAT
GTCAAAGCAAAAGTTGAAGCTGTGTGTGCAAAATTCCCTGTATATGAAAACTAA

Upstream 100 bases:

>100_bases
ATAACTGAGCAACTTCGCCCTGCTTAAACTCAAACTTCATGATACAATAGCGTCCAATCTTAGCACGGCTTAAAAGCCCT
ACTACATAGGACCCTCGCTA

Downstream 100 bases:

>100_bases
GTGATTAGTTTCAGAAAAAGCTCCTAAGGGAGCTTTTTTTATACCTAACATTTATGAATGGAAATTGTTGATTCTAAAAA
ATCATTTGATCCCTGAATTA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFANISLSEFDPELAKSIEAEDARQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPGKRYYGGCEYVDVIEQLAIDR
AKALFGADYANVQPHAGSQANSAVYLALLNAGDTVLGMSLAHGGHLTHGAKVNFSGKTYNAVQYGLNPETGEIDYDEVER
LAIEHKPRMIVAGFSAYSRIVDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPSPVQIADVTTTTTHKTLRGPRSGLIL
AKANEEIEKKLQSAVFPGNQGGPLVHAIAAKAVCFKEAMAPEYKAYQQQVVKNAQAMAEVLIARGYDIVSGGTDNHLFLL
SLIKQDVTGKEADAWLGNANITVNKNAVPNDPRSPFVTSGIRIGTPAVTTRGFGEAEVRDLASWIADVLDSKGDEKVIAD
VKAKVEAVCAKFPVYEN

Sequences:

>Translated_417_residues
MFANISLSEFDPELAKSIEAEDARQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPGKRYYGGCEYVDVIEQLAIDR
AKALFGADYANVQPHAGSQANSAVYLALLNAGDTVLGMSLAHGGHLTHGAKVNFSGKTYNAVQYGLNPETGEIDYDEVER
LAIEHKPRMIVAGFSAYSRIVDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPSPVQIADVTTTTTHKTLRGPRSGLIL
AKANEEIEKKLQSAVFPGNQGGPLVHAIAAKAVCFKEAMAPEYKAYQQQVVKNAQAMAEVLIARGYDIVSGGTDNHLFLL
SLIKQDVTGKEADAWLGNANITVNKNAVPNDPRSPFVTSGIRIGTPAVTTRGFGEAEVRDLASWIADVLDSKGDEKVIAD
VKAKVEAVCAKFPVYEN
>Mature_417_residues
MFANISLSEFDPELAKSIEAEDARQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPGKRYYGGCEYVDVIEQLAIDR
AKALFGADYANVQPHAGSQANSAVYLALLNAGDTVLGMSLAHGGHLTHGAKVNFSGKTYNAVQYGLNPETGEIDYDEVER
LAIEHKPRMIVAGFSAYSRIVDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPSPVQIADVTTTTTHKTLRGPRSGLIL
AKANEEIEKKLQSAVFPGNQGGPLVHAIAAKAVCFKEAMAPEYKAYQQQVVKNAQAMAEVLIARGYDIVSGGTDNHLFLL
SLIKQDVTGKEADAWLGNANITVNKNAVPNDPRSPFVTSGIRIGTPAVTTRGFGEAEVRDLASWIADVLDSKGDEKVIAD
VKAKVEAVCAKFPVYEN

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=452, Percent_Identity=45.1327433628319, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=452, Percent_Identity=45.1327433628319, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=452, Percent_Identity=45.1327433628319, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI19923315, Length=452, Percent_Identity=45.1327433628319, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=452, Percent_Identity=43.8053097345133, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=47.3815461346633, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=44.3298969072165, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1788902, Length=412, Percent_Identity=71.3592233009709, Blast_Score=599, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=45.75, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=45.75, Blast_Score=351, Evalue=5e-97,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=47.6309226932668, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323087, Length=430, Percent_Identity=43.953488372093, Blast_Score=348, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=49.75, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=49.75, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ACIAD (Q6FA66)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046869.1
- ProteinModelPortal:   Q6FA66
- SMR:   Q6FA66
- STRING:   Q6FA66
- GeneID:   2880606
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2255
- NMPDR:   fig|62977.3.peg.2156
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   PEEMNID
- PhylomeDB:   Q6FA66
- ProtClustDB:   PRK00011
- BioCyc:   ASP62977:ACIAD2255-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44943; Mature: 44943

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFANISLSEFDPELAKSIEAEDARQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPG
CCCCCCHHHCCHHHHHHCCHHHHHHHCEEEEEECCCCCCCHHHHCCCCHHHHHHHCCCCC
KRYYGGCEYVDVIEQLAIDRAKALFGADYANVQPHAGSQANSAVYLALLNAGDTVLGMSL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
AHGGHLTHGAKVNFSGKTYNAVQYGLNPETGEIDYDEVERLAIEHKPRMIVAGFSAYSRI
CCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPSPVQIADVTTTTTHKTLRGPRSGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCCEEE
AKANEEIEKKLQSAVFPGNQGGPLVHAIAAKAVCFKEAMAPEYKAYQQQVVKNAQAMAEV
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LIARGYDIVSGGTDNHLFLLSLIKQDVTGKEADAWLGNANITVNKNAVPNDPRSPFVTSG
HHHCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHEECCCEEEECCCCCCCCCCCCCEECC
IRIGTPAVTTRGFGEAEVRDLASWIADVLDSKGDEKVIADVKAKVEAVCAKFPVYEN
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFANISLSEFDPELAKSIEAEDARQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPG
CCCCCCHHHCCHHHHHHCCHHHHHHHCEEEEEECCCCCCCHHHHCCCCHHHHHHHCCCCC
KRYYGGCEYVDVIEQLAIDRAKALFGADYANVQPHAGSQANSAVYLALLNAGDTVLGMSL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
AHGGHLTHGAKVNFSGKTYNAVQYGLNPETGEIDYDEVERLAIEHKPRMIVAGFSAYSRI
CCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPSPVQIADVTTTTTHKTLRGPRSGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCCEEE
AKANEEIEKKLQSAVFPGNQGGPLVHAIAAKAVCFKEAMAPEYKAYQQQVVKNAQAMAEV
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LIARGYDIVSGGTDNHLFLLSLIKQDVTGKEADAWLGNANITVNKNAVPNDPRSPFVTSG
HHHCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHEECCCEEEECCCCCCCCCCCCCEECC
IRIGTPAVTTRGFGEAEVRDLASWIADVLDSKGDEKVIADVKAKVEAVCAKFPVYEN
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA