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The map label for this gene is ppnK

Identifier: 50085338

GI number: 50085338

Start: 2202975

End: 2203898

Strand: Reverse

Name: ppnK

Synonym: ACIAD2231

Alternate gene names: 50085338

Gene position: 2203898-2202975 (Counterclockwise)

Preceding gene: 50085342

Following gene: 50085337

Centisome position: 61.24

GC content: 42.86

Gene sequence:

>924_bases
ATGCAGTCTGCAAATCTTTCCTCCGCCAAATCTTTTCGTAATATTGGTTTGATTGGTCGTCCCGATAAAATATCTGTCGT
TGAAACACTCTGTTTGATCCATGATCACTTAAGAGGGCTTGGACTTCATGTGATTTTTGATCAAGAAACAGCAGAACTCG
TGCCGTATACCAATGTACAAACTGCGAGTCGGTCTTTATTGGGGGAGGTGGTTGATCTTGTGATTGTGGTGGGTGGAGAT
GGTTCTTTACTTCATGCCGCGCGTGCGCTAGTTCGGCATCATACGCCAGTCATTGGAATCAACCGAGGTCGCCTGGGGTT
TCTAACTGATATTAAGCCTGCTGATGCATTGTTTAAACTAGATCAAGTGTTGAAGGGACATTTTCAGTTAGATCGACGTT
TTTTACTTGAAATGGAAGTGAGAACAAAGGGTGAGACACTCTATGATGCGATTGCATTAAATGATGTGGTTTTGCATTCA
GGTAAATCCGTTCACATGATTGATTTCGAATTGCAGATTGATGGACAATATGTATATCGCCAGCATAGTGATGGGCTAAT
TGTATCAACACCAACTGGTTCAACTGCATATGCTTTGTCTGGTGGTGGTCCGATTTTACATCCGAGTATGGATGCTATCG
CACTTGTTCCTATGCATCCGCATACCTTGTCTTCACGGCCCATCGTTGTGGGGGGACAAAGTGAAATTAAATTGACCATA
CGAGAAAATCGGGTACTGCCAATGGTCAGCGCAGATGGTCAACATAGTGTGTCACTGAATGTGGGTGACTGTGTACATAT
CCGTAAACATCCATTTAAGCTAAACTTACTGCATCCACCAGGTTATGATTTTTATATGGCATGCCGTACCAAGCTTGGTT
GGAATCAAGACTTTGACTTAATTAAAGAGCAGGATGACTCATGA

Upstream 100 bases:

>100_bases
TGGGCAAGATTATGGTCTGATCCTGTGTCATGATCAGCCACACTCTTAGAAAATTGATCGCCCAACTAAATTTAATCGAT
AGGCATATTTAAGGCATCCG

Downstream 100 bases:

>100_bases
ATATCGAACAGATGCTTGCCATTTTAGATCCAGACGTTGTTCAACGCTTAAAAATAGCCGTAGAAATTGGAAAATGGCCC
AATGGTGTTGCATTGACAGC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MQSANLSSAKSFRNIGLIGRPDKISVVETLCLIHDHLRGLGLHVIFDQETAELVPYTNVQTASRSLLGEVVDLVIVVGGD
GSLLHAARALVRHHTPVIGINRGRLGFLTDIKPADALFKLDQVLKGHFQLDRRFLLEMEVRTKGETLYDAIALNDVVLHS
GKSVHMIDFELQIDGQYVYRQHSDGLIVSTPTGSTAYALSGGGPILHPSMDAIALVPMHPHTLSSRPIVVGGQSEIKLTI
RENRVLPMVSADGQHSVSLNVGDCVHIRKHPFKLNLLHPPGYDFYMACRTKLGWNQDFDLIKEQDDS

Sequences:

>Translated_307_residues
MQSANLSSAKSFRNIGLIGRPDKISVVETLCLIHDHLRGLGLHVIFDQETAELVPYTNVQTASRSLLGEVVDLVIVVGGD
GSLLHAARALVRHHTPVIGINRGRLGFLTDIKPADALFKLDQVLKGHFQLDRRFLLEMEVRTKGETLYDAIALNDVVLHS
GKSVHMIDFELQIDGQYVYRQHSDGLIVSTPTGSTAYALSGGGPILHPSMDAIALVPMHPHTLSSRPIVVGGQSEIKLTI
RENRVLPMVSADGQHSVSLNVGDCVHIRKHPFKLNLLHPPGYDFYMACRTKLGWNQDFDLIKEQDDS
>Mature_307_residues
MQSANLSSAKSFRNIGLIGRPDKISVVETLCLIHDHLRGLGLHVIFDQETAELVPYTNVQTASRSLLGEVVDLVIVVGGD
GSLLHAARALVRHHTPVIGINRGRLGFLTDIKPADALFKLDQVLKGHFQLDRRFLLEMEVRTKGETLYDAIALNDVVLHS
GKSVHMIDFELQIDGQYVYRQHSDGLIVSTPTGSTAYALSGGGPILHPSMDAIALVPMHPHTLSSRPIVVGGQSEIKLTI
RENRVLPMVSADGQHSVSLNVGDCVHIRKHPFKLNLLHPPGYDFYMACRTKLGWNQDFDLIKEQDDS

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=254, Percent_Identity=31.1023622047244, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1788968, Length=287, Percent_Identity=45.993031358885, Blast_Score=259, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6320794, Length=240, Percent_Identity=32.0833333333333, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6322509, Length=252, Percent_Identity=31.7460317460317, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6325068, Length=276, Percent_Identity=30.7971014492754, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573828, Length=253, Percent_Identity=33.201581027668, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161077047, Length=253, Percent_Identity=33.201581027668, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28573826, Length=253, Percent_Identity=33.201581027668, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28573832, Length=253, Percent_Identity=33.201581027668, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28573830, Length=253, Percent_Identity=33.201581027668, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129957, Length=248, Percent_Identity=31.8548387096774, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653422, Length=248, Percent_Identity=31.8548387096774, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281363323, Length=248, Percent_Identity=31.8548387096774, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281363321, Length=248, Percent_Identity=31.8548387096774, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653424, Length=278, Percent_Identity=30.2158273381295, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_ACIAD (Q6FA87)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046848.1
- ProteinModelPortal:   Q6FA87
- SMR:   Q6FA87
- STRING:   Q6FA87
- GeneID:   2880239
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2231
- NMPDR:   fig|62977.3.peg.2135
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   VANEKRN
- PhylomeDB:   Q6FA87
- ProtClustDB:   PRK01231
- BioCyc:   ASP62977:ACIAD2231-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33823; Mature: 33823

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSANLSSAKSFRNIGLIGRPDKISVVETLCLIHDHLRGLGLHVIFDQETAELVPYTNVQ
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHH
TASRSLLGEVVDLVIVVGGDGSLLHAARALVRHHTPVIGINRGRLGFLTDIKPADALFKL
HHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHH
DQVLKGHFQLDRRFLLEMEVRTKGETLYDAIALNDVVLHSGKSVHMIDFELQIDGQYVYR
HHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHEEEECCCEEEEEEEEEEECCEEEEE
QHSDGLIVSTPTGSTAYALSGGGPILHPSMDAIALVPMHPHTLSSRPIVVGGQSEIKLTI
ECCCCEEEECCCCCEEEEEECCCCEECCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEE
RENRVLPMVSADGQHSVSLNVGDCVHIRKHPFKLNLLHPPGYDFYMACRTKLGWNQDFDL
ECCEEEEEEECCCCEEEEEECCCEEEEECCCEEEEEECCCCCCEEEEEECCCCCCCCCHH
IKEQDDS
HCCCCCC
>Mature Secondary Structure
MQSANLSSAKSFRNIGLIGRPDKISVVETLCLIHDHLRGLGLHVIFDQETAELVPYTNVQ
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHH
TASRSLLGEVVDLVIVVGGDGSLLHAARALVRHHTPVIGINRGRLGFLTDIKPADALFKL
HHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHH
DQVLKGHFQLDRRFLLEMEVRTKGETLYDAIALNDVVLHSGKSVHMIDFELQIDGQYVYR
HHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHEEEECCCEEEEEEEEEEECCEEEEE
QHSDGLIVSTPTGSTAYALSGGGPILHPSMDAIALVPMHPHTLSSRPIVVGGQSEIKLTI
ECCCCEEEECCCCCEEEEEECCCCEECCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEE
RENRVLPMVSADGQHSVSLNVGDCVHIRKHPFKLNLLHPPGYDFYMACRTKLGWNQDFDL
ECCEEEEEEECCCCEEEEEECCCEEEEECCCEEEEEECCCCCCEEEEEECCCCCCCCCHH
IKEQDDS
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA