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The map label for this gene is bioB [H]

Identifier: 50085176

GI number: 50085176

Start: 2032479

End: 2033492

Strand: Direct

Name: bioB [H]

Synonym: ACIAD2045

Alternate gene names: 50085176

Gene position: 2032479-2033492 (Clockwise)

Preceding gene: 50085175

Following gene: 50085177

Centisome position: 56.48

GC content: 40.73

Gene sequence:

>1014_bases
ATGCAATCATCATGGACTATGACAATGACATTACGTAATGATTGGACTCGTGACGAAATCCAAGCACTCTACGATCAACC
TTTTTTAGATTTGGTATTTCAGGCACAACAAGTGCATCGTGAGCATTTTTCTGCCAACACGATTCAAGTAAGTACGTTAT
TATCAATCAAGACAGGAAAATGCCCTGAAGATTGTAAATATTGTTCTCAATCTGCACATTATGATTCCAAATTAGAAGCA
GAAAAGCGTATTGCTGTAGATAAAGTAATTTCTGAAGCAAAAGCTGCAAAAGATTCAGGTTCATCTCGCTTCTGTATGGG
TGCAGCGTGGCGTAACCCTCATGAACGCGATATGCCTTATGTGTTGGAAATGGTACGAGAAGTAAAAGCGTTAGGCTTGG
AAACATGTATGACACTGGGTATGCTGAATCAATCACAAGCCGAGCGTTTAAGTGATGCTGGATTGGATTATTACAATCAT
AATTTAGATACTTCACGTGAATATTACAATAACATTATTAGTACTCGTACTTTTGATGATCGTTTAAATACGCTAGATCA
TGTCCGCAGCGCAGGCATGAAAGTATGTAGTGGTGGTATTGTGGGCTTGGGTGAACAGAAACAAGACCGAATTGGTTTGT
TACATGAGCTTGCTACGCTTCCAATTCATCCTGAGTCTGTACCAATCAATATGTTGGTTCCAATTGAAGGCACCCCATTG
GCTGACGTAGAAAAACTAGATGTAACTGAGTGGATTCGCACAATTGCAGTTGCACGTATTATCATGCCGTATAGCTATAT
TCGCCTTTCTGCTGGCCGTGAATCTTTGAGCGATTCAGATCAAGCGCTTGCTTTTATGGCTGGTGCTAACTCATTATTCT
CTGGCGATAAGTTACTGACGACACCAAATGCAGGTGAAGGTAAAGATCAGGTCTTGTTTGCAAAATTAGGCCTTGTTGCA
GAAAAACCAAAAGTATCTGTGCGCGCTATGGCTGTCGATGCAATGTCAGCTTAA

Upstream 100 bases:

>100_bases
AATTCAACAACAATTAAATAAACAATTGCTATGATATTAAAAAATGATGTTTTTTGCCCTTCGGCTTTGGCACGTTTTCT
GTATGATTGTGCATTAGTAA

Downstream 100 bases:

>100_bases
GTTCAGACGATAAAATAGCCCTCAAATAATTTGAGGGCTGTTTTATATCGATGTCACTAAATTTCCATATTTTGCAGTTA
AATACATCCCTTCTCGAATC

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQSSWTMTMTLRNDWTRDEIQALYDQPFLDLVFQAQQVHREHFSANTIQVSTLLSIKTGKCPEDCKYCSQSAHYDSKLEA
EKRIAVDKVISEAKAAKDSGSSRFCMGAAWRNPHERDMPYVLEMVREVKALGLETCMTLGMLNQSQAERLSDAGLDYYNH
NLDTSREYYNNIISTRTFDDRLNTLDHVRSAGMKVCSGGIVGLGEQKQDRIGLLHELATLPIHPESVPINMLVPIEGTPL
ADVEKLDVTEWIRTIAVARIIMPYSYIRLSAGRESLSDSDQALAFMAGANSLFSGDKLLTTPNAGEGKDQVLFAKLGLVA
EKPKVSVRAMAVDAMSA

Sequences:

>Translated_337_residues
MQSSWTMTMTLRNDWTRDEIQALYDQPFLDLVFQAQQVHREHFSANTIQVSTLLSIKTGKCPEDCKYCSQSAHYDSKLEA
EKRIAVDKVISEAKAAKDSGSSRFCMGAAWRNPHERDMPYVLEMVREVKALGLETCMTLGMLNQSQAERLSDAGLDYYNH
NLDTSREYYNNIISTRTFDDRLNTLDHVRSAGMKVCSGGIVGLGEQKQDRIGLLHELATLPIHPESVPINMLVPIEGTPL
ADVEKLDVTEWIRTIAVARIIMPYSYIRLSAGRESLSDSDQALAFMAGANSLFSGDKLLTTPNAGEGKDQVLFAKLGLVA
EKPKVSVRAMAVDAMSA
>Mature_337_residues
MQSSWTMTMTLRNDWTRDEIQALYDQPFLDLVFQAQQVHREHFSANTIQVSTLLSIKTGKCPEDCKYCSQSAHYDSKLEA
EKRIAVDKVISEAKAAKDSGSSRFCMGAAWRNPHERDMPYVLEMVREVKALGLETCMTLGMLNQSQAERLSDAGLDYYNH
NLDTSREYYNNIISTRTFDDRLNTLDHVRSAGMKVCSGGIVGLGEQKQDRIGLLHELATLPIHPESVPINMLVPIEGTPL
ADVEKLDVTEWIRTIAVARIIMPYSYIRLSAGRESLSDSDQALAFMAGANSLFSGDKLLTTPNAGEGKDQVLFAKLGLVA
EKPKVSVRAMAVDAMSA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=317, Percent_Identity=63.7223974763407, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=49.6815286624204, Blast_Score=337, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37458; Mature: 37458

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSSWTMTMTLRNDWTRDEIQALYDQPFLDLVFQAQQVHREHFSANTIQVSTLLSIKTGK
CCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCC
CPEDCKYCSQSAHYDSKLEAEKRIAVDKVISEAKAAKDSGSSRFCMGAAWRNPHERDMPY
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHH
VLEMVREVKALGLETCMTLGMLNQSQAERLSDAGLDYYNHNLDTSREYYNNIISTRTFDD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHCCCHHH
RLNTLDHVRSAGMKVCSGGIVGLGEQKQDRIGLLHELATLPIHPESVPINMLVPIEGTPL
HHHHHHHHHHHCHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
ADVEKLDVTEWIRTIAVARIIMPYSYIRLSAGRESLSDSDQALAFMAGANSLFSGDKLLT
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHCCCCHHHEEHHHCCCCCCCCCEEEE
TPNAGEGKDQVLFAKLGLVAEKPKVSVRAMAVDAMSA
CCCCCCCCCCEEHHHHCHHCCCCCCEEEEEEHHHHCC
>Mature Secondary Structure
MQSSWTMTMTLRNDWTRDEIQALYDQPFLDLVFQAQQVHREHFSANTIQVSTLLSIKTGK
CCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCC
CPEDCKYCSQSAHYDSKLEAEKRIAVDKVISEAKAAKDSGSSRFCMGAAWRNPHERDMPY
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHH
VLEMVREVKALGLETCMTLGMLNQSQAERLSDAGLDYYNHNLDTSREYYNNIISTRTFDD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHCCCHHH
RLNTLDHVRSAGMKVCSGGIVGLGEQKQDRIGLLHELATLPIHPESVPINMLVPIEGTPL
HHHHHHHHHHHCHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
ADVEKLDVTEWIRTIAVARIIMPYSYIRLSAGRESLSDSDQALAFMAGANSLFSGDKLLT
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHCCCCHHHEEHHHCCCCCCCCCEEEE
TPNAGEGKDQVLFAKLGLVAEKPKVSVRAMAVDAMSA
CCCCCCCCCCEEHHHHCHHCCCCCCEEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA