Click here to switch to the map view.

The map label for this gene is fruA [H]

Identifier: 50085129

GI number: 50085129

Start: 1984979

End: 1986706

Strand: Direct

Name: fruA [H]

Synonym: ACIAD1993

Alternate gene names: 50085129

Gene position: 1984979-1986706 (Clockwise)

Preceding gene: 50085128

Following gene: 50085130

Centisome position: 55.16

GC content: 42.19

Gene sequence:

>1728_bases
CTGATGCAAACACTTAAATCTCTTTTATTTGTGATCAATAGCACGCAACAGTATGTCAATGCTTCGATTTTGGCAAAGAA
ATTAACGCAAGCTGCTGCTAAATCGGGGCTGGAAAGTAAAACAATAACAATTAAAGATTCAGTTCCAAATCAAGAATTTG
ATTTGGTTATCTTTATTGGTCAGCTACCCAAGAATCTTTCAACATTCAATGCTGTAAAATTTTCTCAGATTGATCTTGGA
AATGCCAATCAAAATGCGGATGAAGTACTTCAACAGGCATTTCAGCATTCAAAAAGCCCAGATGAACTCAACGCTACCAC
GTCCAATTCGGCTCAGAAAGCCCAACGTTTTGTAGCGATTACGGCCTGTCCAACAGGTGTAGCACACACGTTTATGGCCG
CAGAAGCCTTACAGCAAGGCGCGGATAGACTCGGTTACATCATTGATGTAGAAACACAAGGCTCTGTTGGAGCAAAAAAT
ATTCTATCTGCCGAAGCCATTGAAGCCGCAGATATTGTCATTTTGGCAACAGATATCGAAGTCAATACAGATCGTTTTGT
AGGTAAACGGGTTTACCGTTGCAGCACAGGTTTTGCTTTAAAACAAACAGATAAAGCTTTTGATCAGGCAATTGCCGAAG
CAAAACTACTTGAATCATCCAAGCAAGCATCTAGCAATGCCACAGGCTCTACCAACAAAGAAAAAACAGGCATTTATAAA
CACTTATTGACTGGTGTTTCTTTTATGCTTCCAATGGTGGTGGCAGGTGGCTTGCTCATTGCACTTTCACTCTGTTTTGG
TTTAAATGCTGCCGAACAAAGCGGTAGCTTGGCCTATGTATTAAAACAAATTGGGGTTACAGCATTTACCCTAATGGTTC
CCATGCTTTCTGGATATATTGCGTATTCAATTGCAGATCGTCCAGGTTTGACCCCAGGCCTGATTGGTGGACTCCTCGCA
ACTCAATTACAAGCAGGCTTTCTTGGTGGGATTGTTTCTGGATTTCTGGCAGGATACATTGCATTATTCCTTGCCAAAAA
AATCAAGCTCCCAACGAGCCTTGAATCATTAAAGCCAATTCTGATTGTACCTTTGCTCGGAACACTTTCTGTCGGTCTGA
TCATGTATTATGTCGTTGGTCATCCAGTGGCTCAGATCTTTGAGATGATGAAAGACTTCCTAAATAACATGGGTACCACC
AATGCTGTATTAATGGGCATTGTACTTGCCTCAATGATGTGTATCGACCTTGGTGGTCCAATCAATAAAGCAGCCTACGC
GTTTACCGTAGGTCTACTGACCACCAACACGTATATGCCGATGGCAGCAACAATGGCAGGCGGTATGGTTCCTGCAATTG
GTATGGCTTTAGCAACCTTTTTTGCCAAAAACAAATTTAATATAGGTGAGCGCGATGCAGGTAAAGCCGCATTTGTATTA
GGTCTTTGCTTTATTTCCGAGGGTGCAATTCCTTTTGCTGCAAAAGATCCAATGCGTGTTATCCCCACTTGTATTGCGGG
TGGCGCCGTTACTGGTGCTTTGGTTGCGCTATTCCATTGTGAGCTGGTAACACCACATGGCGGGGTATTTGTACTACTCA
TTCCAAATGCGATTAACCATGCTTGGTTATATCTGATTGCAATTGCAGCAGGCAGTTTGGTTACAGGTATTAGTTATGCT
GTTTTAAAACGTCGTGAGACGACAGCATTACAAAGTCAAACTGTATAA

Upstream 100 bases:

>100_bases
ATTGCAAGCCATGCAGTAAGTCAGGTCGGATTTAGAATTCCGACTCAAGATAAACTTGCTCATCTCAAACAACAAACCAC
TATCAAATCATTGAGTTAAT

Downstream 100 bases:

>100_bases
AACTTTAAAAGTTCAAGCAAGAAAAGAGAGCTCAGGCTCTCTTTTTTATGTCAAGATGTTCGATCTATATCATGCTTTAA
CATTGTTATTAATGAAATGA

Product: phosphotransferase system, fructose-specific IIBC component

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MMQTLKSLLFVINSTQQYVNASILAKKLTQAAAKSGLESKTITIKDSVPNQEFDLVIFIGQLPKNLSTFNAVKFSQIDLG
NANQNADEVLQQAFQHSKSPDELNATTSNSAQKAQRFVAITACPTGVAHTFMAAEALQQGADRLGYIIDVETQGSVGAKN
ILSAEAIEAADIVILATDIEVNTDRFVGKRVYRCSTGFALKQTDKAFDQAIAEAKLLESSKQASSNATGSTNKEKTGIYK
HLLTGVSFMLPMVVAGGLLIALSLCFGLNAAEQSGSLAYVLKQIGVTAFTLMVPMLSGYIAYSIADRPGLTPGLIGGLLA
TQLQAGFLGGIVSGFLAGYIALFLAKKIKLPTSLESLKPILIVPLLGTLSVGLIMYYVVGHPVAQIFEMMKDFLNNMGTT
NAVLMGIVLASMMCIDLGGPINKAAYAFTVGLLTTNTYMPMAATMAGGMVPAIGMALATFFAKNKFNIGERDAGKAAFVL
GLCFISEGAIPFAAKDPMRVIPTCIAGGAVTGALVALFHCELVTPHGGVFVLLIPNAINHAWLYLIAIAAGSLVTGISYA
VLKRRETTALQSQTV

Sequences:

>Translated_575_residues
MMQTLKSLLFVINSTQQYVNASILAKKLTQAAAKSGLESKTITIKDSVPNQEFDLVIFIGQLPKNLSTFNAVKFSQIDLG
NANQNADEVLQQAFQHSKSPDELNATTSNSAQKAQRFVAITACPTGVAHTFMAAEALQQGADRLGYIIDVETQGSVGAKN
ILSAEAIEAADIVILATDIEVNTDRFVGKRVYRCSTGFALKQTDKAFDQAIAEAKLLESSKQASSNATGSTNKEKTGIYK
HLLTGVSFMLPMVVAGGLLIALSLCFGLNAAEQSGSLAYVLKQIGVTAFTLMVPMLSGYIAYSIADRPGLTPGLIGGLLA
TQLQAGFLGGIVSGFLAGYIALFLAKKIKLPTSLESLKPILIVPLLGTLSVGLIMYYVVGHPVAQIFEMMKDFLNNMGTT
NAVLMGIVLASMMCIDLGGPINKAAYAFTVGLLTTNTYMPMAATMAGGMVPAIGMALATFFAKNKFNIGERDAGKAAFVL
GLCFISEGAIPFAAKDPMRVIPTCIAGGAVTGALVALFHCELVTPHGGVFVLLIPNAINHAWLYLIAIAAGSLVTGISYA
VLKRRETTALQSQTV
>Mature_575_residues
MMQTLKSLLFVINSTQQYVNASILAKKLTQAAAKSGLESKTITIKDSVPNQEFDLVIFIGQLPKNLSTFNAVKFSQIDLG
NANQNADEVLQQAFQHSKSPDELNATTSNSAQKAQRFVAITACPTGVAHTFMAAEALQQGADRLGYIIDVETQGSVGAKN
ILSAEAIEAADIVILATDIEVNTDRFVGKRVYRCSTGFALKQTDKAFDQAIAEAKLLESSKQASSNATGSTNKEKTGIYK
HLLTGVSFMLPMVVAGGLLIALSLCFGLNAAEQSGSLAYVLKQIGVTAFTLMVPMLSGYIAYSIADRPGLTPGLIGGLLA
TQLQAGFLGGIVSGFLAGYIALFLAKKIKLPTSLESLKPILIVPLLGTLSVGLIMYYVVGHPVAQIFEMMKDFLNNMGTT
NAVLMGIVLASMMCIDLGGPINKAAYAFTVGLLTTNTYMPMAATMAGGMVPAIGMALATFFAKNKFNIGERDAGKAAFVL
GLCFISEGAIPFAAKDPMRVIPTCIAGGAVTGALVALFHCELVTPHGGVFVLLIPNAINHAWLYLIAIAAGSLVTGISYA
VLKRRETTALQSQTV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=564, Percent_Identity=53.3687943262411, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1786951, Length=557, Percent_Identity=33.572710951526, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082348, Length=485, Percent_Identity=36.4948453608247, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790386, Length=282, Percent_Identity=41.1347517730496, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1788729, Length=399, Percent_Identity=28.3208020050125, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1790387, Length=90, Percent_Identity=48.8888888888889, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788730, Length=89, Percent_Identity=39.3258426966292, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790390, Length=107, Percent_Identity=38.3177570093458, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 60663; Mature: 60663

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQTLKSLLFVINSTQQYVNASILAKKLTQAAAKSGLESKTITIKDSVPNQEFDLVIFIG
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEC
QLPKNLSTFNAVKFSQIDLGNANQNADEVLQQAFQHSKSPDELNATTSNSAQKAQRFVAI
CCCCCCHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEE
TACPTGVAHTFMAAEALQQGADRLGYIIDVETQGSVGAKNILSAEAIEAADIVILATDIE
EECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCE
VNTDRFVGKRVYRCSTGFALKQTDKAFDQAIAEAKLLESSKQASSNATGSTNKEKTGIYK
ECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
HLLTGVSFMLPMVVAGGLLIALSLCFGLNAAEQSGSLAYVLKQIGVTAFTLMVPMLSGYI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYSIADRPGLTPGLIGGLLATQLQAGFLGGIVSGFLAGYIALFLAKKIKLPTSLESLKPI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCE
LIVPLLGTLSVGLIMYYVVGHPVAQIFEMMKDFLNNMGTTNAVLMGIVLASMMCIDLGGP
EEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
INKAAYAFTVGLLTTNTYMPMAATMAGGMVPAIGMALATFFAKNKFNIGERDAGKAAFVL
CCHHHHHHEEEHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
GLCFISEGAIPFAAKDPMRVIPTCIAGGAVTGALVALFHCELVTPHGGVFVLLIPNAINH
HHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHH
AWLYLIAIAAGSLVTGISYAVLKRRETTALQSQTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMQTLKSLLFVINSTQQYVNASILAKKLTQAAAKSGLESKTITIKDSVPNQEFDLVIFIG
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEC
QLPKNLSTFNAVKFSQIDLGNANQNADEVLQQAFQHSKSPDELNATTSNSAQKAQRFVAI
CCCCCCHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEE
TACPTGVAHTFMAAEALQQGADRLGYIIDVETQGSVGAKNILSAEAIEAADIVILATDIE
EECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCE
VNTDRFVGKRVYRCSTGFALKQTDKAFDQAIAEAKLLESSKQASSNATGSTNKEKTGIYK
ECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
HLLTGVSFMLPMVVAGGLLIALSLCFGLNAAEQSGSLAYVLKQIGVTAFTLMVPMLSGYI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYSIADRPGLTPGLIGGLLATQLQAGFLGGIVSGFLAGYIALFLAKKIKLPTSLESLKPI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCE
LIVPLLGTLSVGLIMYYVVGHPVAQIFEMMKDFLNNMGTTNAVLMGIVLASMMCIDLGGP
EEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
INKAAYAFTVGLLTTNTYMPMAATMAGGMVPAIGMALATFFAKNKFNIGERDAGKAAFVL
CCHHHHHHEEEHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
GLCFISEGAIPFAAKDPMRVIPTCIAGGAVTGALVALFHCELVTPHGGVFVLLIPNAINH
HHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHH
AWLYLIAIAAGSLVTGISYAVLKRRETTALQSQTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]