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The map label for this gene is glnS [H]

Identifier: 50085060

GI number: 50085060

Start: 1910414

End: 1912141

Strand: Reverse

Name: glnS [H]

Synonym: ACIAD1920

Alternate gene names: 50085060

Gene position: 1912141-1910414 (Counterclockwise)

Preceding gene: 50085064

Following gene: 50085057

Centisome position: 53.14

GC content: 42.59

Gene sequence:

>1728_bases
ATGAAGCCAAATGATGTTGTTTCAACTCTGCCCAATTCTCCGACAACGAATACTCATACATCTGTCGATTCTGCGCAGCA
AGAACAACAGGCAGGCTTAGATTTTGTCAGACAAGTCATCACAGAAGATTTGAACGAAGGTCGTACCCAAACTGTGGTCA
CTCGTTTTCCACCTGAACCAAACGGTTATTTGCATATTGGTCATGTTAAGGCCATTTGTCTTAATTTTGGCGTTGCTGAA
GAATTTGGTGGTTATTGTAATCTCAGATTTGATGATACCAACCCAGATGCAGAAGAGCAGGAATATGTCGATGGGATTGC
CAACGACGTAAAATGGCTTGGTTTTGAATGGAAAGATGAACCACGTTATGCATCGAGCTACTTCGACCAGCTTTATACGT
GGGCGGTACAACTGATTAAACAAGGTGATGCCTATGTCGACTTGCAAACACCCGAAGAAATTAAGCTGAACCGTGGTAAC
TTTGTAGAGCCAGGTAAAAACTCTCCTTACCGTGATGCGAGTGTAGAAGAAAACCTTCAACATTTTGAAAAAATGAAAGA
TGGGACTTATCAGGAAGGTCAAGCTGTTTTACGTGCCAAAATTGATATGGCAAGCCCGAATGTACATATGCGTGATCCAA
TTTTGTATCGTATTCTGCATTCAGAGCACCATCAAACAGGTGATACATGGAAAATCTATCCAATGTATGACTATGCGCAT
CCGTTATCTGATGCGATTGAAGGTGTTACGCATTCGTTATGTACGCTTGAGTTCCAAGATCATCGTCCATTTTATGACTG
GGTGATTGAAAAAGTTCATTCAGAAGCAGTACCACGCCAGTATGAATCATCTCGTTTAAACATCGATTACACCATTACCT
CTAAGCGTAAGTTACGCCGTCTGGTCGAAGGTGGTTTTGTAAATGGTTGGGATGATCCGCGTATGCCAACAGTCGTCGGT
ATGCGCCGTCGTGGATTTACCTCTGAAGGCTTGCGTGATTTTTGTAAGCGTGTAGGTGTATCTAAAACGGATGGTATTGT
GGATGTTGCAATGCTCGAATTCTGTATTCGTCAGTCGCTCGAAAATACTGCAAGCCGTGGCATGGCTGTACTCAATCCGT
TGAAAGTCACCTTAACCAATTTGCCAGAAACACTTGATCTTGTGCATGCACGCCATCCGAATGTCGATATGGGCGAACGT
GTTATTCCACTAACATCTGAGCTTTATATTGACCGCAAAGACTTTGAAGAAGTGCCACCAAAAGGCTTTAAACGTTTGAT
TCCAGGTGGTGAAGTACGTTTGCGTCATGCTTACGTGATCAAATGCGATGAAGTGGTCAAAGATGAACAGGGTGAAGTCA
TTGAGCTTAAATGTTCAATTGATCCAGACACTTTAGGTAAAAACCCCGAAGGTCGTAAGGTAAAAGGGGTGATTCATTGG
GTATCTGCAACCAAAGGACTTCCTGCGGAAGTGCGTATTTATGATCGCCTATTTACAGAAGCTGCACCTGATGCAGATGA
TGACTTTCTTGCCAATTTGAATCCTGACTCATTACAAGTCGTTCAGGCCTATATTGAGCCAGCATTGGCACAAGCATCAC
CTGAACAACGCTTTCAGTTTGAGCGCGAAGGTTATTTTGTAGCAGATCAGCATGATCACACTTCAGAAAAACCAGTGTTT
AACCGTATTTTAGACCTAAAAGATAGCTTCAAACCTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
GTAAACTACATAGCAAGTAAAAAACGTAAATTTCTTTGATAAAAACAAAGATTACGTTTCATTCAGTCAAATTTTAACTG
TAAAGAAGTGAAATATTTAT

Downstream 100 bases:

>100_bases
GGCGACAGCCTTATTTTTTCGCAAGGCCCCGCAGGGTAAAGCGCGACAGTCTTATTTTTTAGCAAGGTCCCGCAGGGAAA
TGTAGCAACGCTTTACCTCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKPNDVVSTLPNSPTTNTHTSVDSAQQEQQAGLDFVRQVITEDLNEGRTQTVVTRFPPEPNGYLHIGHVKAICLNFGVAE
EFGGYCNLRFDDTNPDAEEQEYVDGIANDVKWLGFEWKDEPRYASSYFDQLYTWAVQLIKQGDAYVDLQTPEEIKLNRGN
FVEPGKNSPYRDASVEENLQHFEKMKDGTYQEGQAVLRAKIDMASPNVHMRDPILYRILHSEHHQTGDTWKIYPMYDYAH
PLSDAIEGVTHSLCTLEFQDHRPFYDWVIEKVHSEAVPRQYESSRLNIDYTITSKRKLRRLVEGGFVNGWDDPRMPTVVG
MRRRGFTSEGLRDFCKRVGVSKTDGIVDVAMLEFCIRQSLENTASRGMAVLNPLKVTLTNLPETLDLVHARHPNVDMGER
VIPLTSELYIDRKDFEEVPPKGFKRLIPGGEVRLRHAYVIKCDEVVKDEQGEVIELKCSIDPDTLGKNPEGRKVKGVIHW
VSATKGLPAEVRIYDRLFTEAAPDADDDFLANLNPDSLQVVQAYIEPALAQASPEQRFQFEREGYFVADQHDHTSEKPVF
NRILDLKDSFKPEKK

Sequences:

>Translated_575_residues
MKPNDVVSTLPNSPTTNTHTSVDSAQQEQQAGLDFVRQVITEDLNEGRTQTVVTRFPPEPNGYLHIGHVKAICLNFGVAE
EFGGYCNLRFDDTNPDAEEQEYVDGIANDVKWLGFEWKDEPRYASSYFDQLYTWAVQLIKQGDAYVDLQTPEEIKLNRGN
FVEPGKNSPYRDASVEENLQHFEKMKDGTYQEGQAVLRAKIDMASPNVHMRDPILYRILHSEHHQTGDTWKIYPMYDYAH
PLSDAIEGVTHSLCTLEFQDHRPFYDWVIEKVHSEAVPRQYESSRLNIDYTITSKRKLRRLVEGGFVNGWDDPRMPTVVG
MRRRGFTSEGLRDFCKRVGVSKTDGIVDVAMLEFCIRQSLENTASRGMAVLNPLKVTLTNLPETLDLVHARHPNVDMGER
VIPLTSELYIDRKDFEEVPPKGFKRLIPGGEVRLRHAYVIKCDEVVKDEQGEVIELKCSIDPDTLGKNPEGRKVKGVIHW
VSATKGLPAEVRIYDRLFTEAAPDADDDFLANLNPDSLQVVQAYIEPALAQASPEQRFQFEREGYFVADQHDHTSEKPVF
NRILDLKDSFKPEKK
>Mature_575_residues
MKPNDVVSTLPNSPTTNTHTSVDSAQQEQQAGLDFVRQVITEDLNEGRTQTVVTRFPPEPNGYLHIGHVKAICLNFGVAE
EFGGYCNLRFDDTNPDAEEQEYVDGIANDVKWLGFEWKDEPRYASSYFDQLYTWAVQLIKQGDAYVDLQTPEEIKLNRGN
FVEPGKNSPYRDASVEENLQHFEKMKDGTYQEGQAVLRAKIDMASPNVHMRDPILYRILHSEHHQTGDTWKIYPMYDYAH
PLSDAIEGVTHSLCTLEFQDHRPFYDWVIEKVHSEAVPRQYESSRLNIDYTITSKRKLRRLVEGGFVNGWDDPRMPTVVG
MRRRGFTSEGLRDFCKRVGVSKTDGIVDVAMLEFCIRQSLENTASRGMAVLNPLKVTLTNLPETLDLVHARHPNVDMGER
VIPLTSELYIDRKDFEEVPPKGFKRLIPGGEVRLRHAYVIKCDEVVKDEQGEVIELKCSIDPDTLGKNPEGRKVKGVIHW
VSATKGLPAEVRIYDRLFTEAAPDADDDFLANLNPDSLQVVQAYIEPALAQASPEQRFQFEREGYFVADQHDHTSEKPVF
NRILDLKDSFKPEKK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=528, Percent_Identity=45.6439393939394, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI62241042, Length=504, Percent_Identity=34.7222222222222, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1786895, Length=540, Percent_Identity=57.5925925925926, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=344, Percent_Identity=23.2558139534884, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=531, Percent_Identity=43.1261770244821, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17511007, Length=519, Percent_Identity=33.140655105973, Blast_Score=243, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324742, Length=563, Percent_Identity=41.2078152753108, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321192, Length=537, Percent_Identity=36.6852886405959, Blast_Score=300, Evalue=5e-82,
Organism=Drosophila melanogaster, GI17864488, Length=527, Percent_Identity=43.2637571157495, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24649466, Length=515, Percent_Identity=34.368932038835, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 65647; Mature: 65647

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNDVVSTLPNSPTTNTHTSVDSAQQEQQAGLDFVRQVITEDLNEGRTQTVVTRFPPEP
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NGYLHIGHVKAICLNFGVAEEFGGYCNLRFDDTNPDAEEQEYVDGIANDVKWLGFEWKDE
CCEEEECHHHHHHHHCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PRYASSYFDQLYTWAVQLIKQGDAYVDLQTPEEIKLNRGNFVEPGKNSPYRDASVEENLQ
CCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHEEECCCCCCCCCCCCCCCCCCHHHHHH
HFEKMKDGTYQEGQAVLRAKIDMASPNVHMRDPILYRILHSEHHQTGDTWKIYPMYDYAH
HHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHC
PLSDAIEGVTHSLCTLEFQDHRPFYDWVIEKVHSEAVPRQYESSRLNIDYTITSKRKLRR
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCHHHHHH
LVEGGFVNGWDDPRMPTVVGMRRRGFTSEGLRDFCKRVGVSKTDGIVDVAMLEFCIRQSL
HHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ENTASRGMAVLNPLKVTLTNLPETLDLVHARHPNVDMGERVIPLTSELYIDRKDFEEVPP
HHHHHCCEEHHCCHHHEECCCCHHHHHHHCCCCCCCCCCEEECCHHHHEECHHHHHHCCC
KGFKRLIPGGEVRLRHAYVIKCDEVVKDEQGEVIELKCSIDPDTLGKNPEGRKVKGVIHW
HHHHHCCCCCCEEEEEEEEEEEHHHHCCCCCCEEEEEECCCHHHCCCCCCCCEEEHEEEE
VSATKGLPAEVRIYDRLFTEAAPDADDDFLANLNPDSLQVVQAYIEPALAQASPEQRFQF
EHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
EREGYFVADQHDHTSEKPVFNRILDLKDSFKPEKK
HCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKPNDVVSTLPNSPTTNTHTSVDSAQQEQQAGLDFVRQVITEDLNEGRTQTVVTRFPPEP
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NGYLHIGHVKAICLNFGVAEEFGGYCNLRFDDTNPDAEEQEYVDGIANDVKWLGFEWKDE
CCEEEECHHHHHHHHCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PRYASSYFDQLYTWAVQLIKQGDAYVDLQTPEEIKLNRGNFVEPGKNSPYRDASVEENLQ
CCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHEEECCCCCCCCCCCCCCCCCCHHHHHH
HFEKMKDGTYQEGQAVLRAKIDMASPNVHMRDPILYRILHSEHHQTGDTWKIYPMYDYAH
HHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHC
PLSDAIEGVTHSLCTLEFQDHRPFYDWVIEKVHSEAVPRQYESSRLNIDYTITSKRKLRR
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCHHHHHH
LVEGGFVNGWDDPRMPTVVGMRRRGFTSEGLRDFCKRVGVSKTDGIVDVAMLEFCIRQSL
HHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ENTASRGMAVLNPLKVTLTNLPETLDLVHARHPNVDMGERVIPLTSELYIDRKDFEEVPP
HHHHHCCEEHHCCHHHEECCCCHHHHHHHCCCCCCCCCCEEECCHHHHEECHHHHHHCCC
KGFKRLIPGGEVRLRHAYVIKCDEVVKDEQGEVIELKCSIDPDTLGKNPEGRKVKGVIHW
HHHHHCCCCCCEEEEEEEEEEEHHHHCCCCCCEEEEEECCCHHHCCCCCCCCEEEHEEEE
VSATKGLPAEVRIYDRLFTEAAPDADDDFLANLNPDSLQVVQAYIEPALAQASPEQRFQF
EHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
EREGYFVADQHDHTSEKPVFNRILDLKDSFKPEKK
HCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA