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The map label for this gene is bigR [H]

Identifier: 50085009

GI number: 50085009

Start: 1860447

End: 1860755

Strand: Direct

Name: bigR [H]

Synonym: ACIAD1865

Alternate gene names: 50085009

Gene position: 1860447-1860755 (Clockwise)

Preceding gene: 50085008

Following gene: 50085010

Centisome position: 51.7

GC content: 30.42

Gene sequence:

>309_bases
ATGAGTAAAGAGCTTGAAATAGATTTAATTCGTCAATCTGCTGATGCAGTGGTTACTATATTAAAATCTCTAGCTAATAC
AGATCGGCTACTTATTTTGTGTTATTTGGCAAAACAAGAATTAAATGTTTCTCAAATTGAGGAAAAGACCAATATTACTC
AACCTACTCTATCACAACAATTAATGATGCTTAGAAAAAGTGACGTGGTTCATACTCGCCGTGACGGCAAGCAAATTTTT
TATTCAATTAAAGATGAAAAATTAATTATTGTTCTATCAACTTTATATGAACTATATTGCCCTCAATAA

Upstream 100 bases:

>100_bases
AATTGATGTCAGCTTAGCTTATTTAGATATTTAAAATATATACACTCCTCATAAGTTTTAGACTTATAATTCAGACTTTC
AAATGTTTAGAGATCTGTAA

Downstream 100 bases:

>100_bases
ATATTTCTTATTTTAAAGTATGGATATTGCGCTAGATAGCCATCTTAAGCCGAGAAAAGTAAATACTTAGCCTTAAAACC
AGTGTAAATTGATCTAAAAA

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 102; Mature: 101

Protein sequence:

>102_residues
MSKELEIDLIRQSADAVVTILKSLANTDRLLILCYLAKQELNVSQIEEKTNITQPTLSQQLMMLRKSDVVHTRRDGKQIF
YSIKDEKLIIVLSTLYELYCPQ

Sequences:

>Translated_102_residues
MSKELEIDLIRQSADAVVTILKSLANTDRLLILCYLAKQELNVSQIEEKTNITQPTLSQQLMMLRKSDVVHTRRDGKQIF
YSIKDEKLIIVLSTLYELYCPQ
>Mature_101_residues
SKELEIDLIRQSADAVVTILKSLANTDRLLILCYLAKQELNVSQIEEKTNITQPTLSQQLMMLRKSDVVHTRRDGKQIFY
SIKDEKLIIVLSTLYELYCPQ

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=97, Percent_Identity=30.9278350515464, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 11800; Mature: 11669

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELEIDLIRQSADAVVTILKSLANTDRLLILCYLAKQELNVSQIEEKTNITQPTLSQQ
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHEEHHHCCCCHHHHHHHCCCCCCHHHHH
LMMLRKSDVVHTRRDGKQIFYSIKDEKLIIVLSTLYELYCPQ
HHHHHHHCCHHCCCCCCEEEEEECCCEEEEEHHHHHHHHCCC
>Mature Secondary Structure 
SKELEIDLIRQSADAVVTILKSLANTDRLLILCYLAKQELNVSQIEEKTNITQPTLSQQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHEEHHHCCCCHHHHHHHCCCCCCHHHHH
LMMLRKSDVVHTRRDGKQIFYSIKDEKLIIVLSTLYELYCPQ
HHHHHHHCCHHCCCCCCEEEEEECCCEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]