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The map label for this gene is rutF [H]

Identifier: 50084973

GI number: 50084973

Start: 1834181

End: 1834837

Strand: Direct

Name: rutF [H]

Synonym: ACIAD1827

Alternate gene names: 50084973

Gene position: 1834181-1834837 (Clockwise)

Preceding gene: 50084972

Following gene: 50084974

Centisome position: 50.97

GC content: 44.14

Gene sequence:

>657_bases
GTGAAACTCATGTGCAACTCACGAAATGCGTTGCACATGCCTTATAAACAAGCCAATGTAATAAAAAGCGAACAATTTAG
TCCAGGGAACTATGAGGATTACACCATGTCTGTTGAAGCACAATTGCAGTCCCGTCTAAGTCATACTACAGACTTGATCG
ATACCTACCAAAGTATTGATATCGCTAGATTTAAACAATCTATGCGCCATATTGCCTCGCCGGTTGGCGTGGTGACTACA
CATCAAGGTGACTTACGCCATGGTTTAACGGCCACAGCCATTTGTTCAGTCTCGATGGAACCACCCAGTATGTTGGTATG
CGTAAACCGCAATGCCAGCGCAGAGCAAATTATTGCCCGAAGCGGAAGTTTTGCGATCAATATGCTTACCGATGAACAAC
ATGGAATAGCACGCTTATTTTCTACACCGGGTACAACACCAGAGCAACGCTATACTGAAGGCGAGTGGCATACGTTGGTA
ACTGGTTCTCCAATTTTAAAAGGTGCTGTTGCAGCATTTGATTGCGTGGTTGAGGAATGTATTCAGTCGGGAACACATCA
TCTCTATATTGGTCGAGTGCTGGCTGCAGAATATCTGGATCAAAATATTCTACTGTACCGAGATGGCCTGTTTCGTAAGC
TTGAACGTATCATTTGA

Upstream 100 bases:

>100_bases
TATTGATTTAAACCCAGTGCTGGTACTCGATCAAGACCAAGGCGTAATCGCCCTAGATGCGCTGATTGTGCCGCGATCAG
CTCAGCCATAATTCATAAAT

Downstream 100 bases:

>100_bases
TCAGACACGATGGTGATCAATATGAACACTCAAAGAAAAGTTGCGATCGTGACTGGGGCAAACGGCGGTATTGGACGAGT
AATTTGTCAATCGTTGGCCA

Product: flavoprotein oxidoreductase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKLMCNSRNALHMPYKQANVIKSEQFSPGNYEDYTMSVEAQLQSRLSHTTDLIDTYQSIDIARFKQSMRHIASPVGVVTT
HQGDLRHGLTATAICSVSMEPPSMLVCVNRNASAEQIIARSGSFAINMLTDEQHGIARLFSTPGTTPEQRYTEGEWHTLV
TGSPILKGAVAAFDCVVEECIQSGTHHLYIGRVLAAEYLDQNILLYRDGLFRKLERII

Sequences:

>Translated_218_residues
MKLMCNSRNALHMPYKQANVIKSEQFSPGNYEDYTMSVEAQLQSRLSHTTDLIDTYQSIDIARFKQSMRHIASPVGVVTT
HQGDLRHGLTATAICSVSMEPPSMLVCVNRNASAEQIIARSGSFAINMLTDEQHGIARLFSTPGTTPEQRYTEGEWHTLV
TGSPILKGAVAAFDCVVEECIQSGTHHLYIGRVLAAEYLDQNILLYRDGLFRKLERII
>Mature_218_residues
MKLMCNSRNALHMPYKQANVIKSEQFSPGNYEDYTMSVEAQLQSRLSHTTDLIDTYQSIDIARFKQSMRHIASPVGVVTT
HQGDLRHGLTATAICSVSMEPPSMLVCVNRNASAEQIIARSGSFAINMLTDEQHGIARLFSTPGTTPEQRYTEGEWHTLV
TGSPILKGAVAAFDCVVEECIQSGTHHLYIGRVLAAEYLDQNILLYRDGLFRKLERII

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=131, Percent_Identity=35.8778625954198, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 24318; Mature: 24318

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMCNSRNALHMPYKQANVIKSEQFSPGNYEDYTMSVEAQLQSRLSHTTDLIDTYQSID
CCEEECCCCCEECCHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
IARFKQSMRHIASPVGVVTTHQGDLRHGLTATAICSVSMEPPSMLVCVNRNASAEQIIAR
HHHHHHHHHHHCCCCCEEEECCCCHHHCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHC
SGSFAINMLTDEQHGIARLFSTPGTTPEQRYTEGEWHTLVTGSPILKGAVAAFDCVVEEC
CCCEEEEEECCCCCHHHHHHCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
IQSGTHHLYIGRVLAAEYLDQNILLYRDGLFRKLERII
HHCCCCEEEHHHHHHHHHHCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MKLMCNSRNALHMPYKQANVIKSEQFSPGNYEDYTMSVEAQLQSRLSHTTDLIDTYQSID
CCEEECCCCCEECCHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
IARFKQSMRHIASPVGVVTTHQGDLRHGLTATAICSVSMEPPSMLVCVNRNASAEQIIAR
HHHHHHHHHHHCCCCCEEEECCCCHHHCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHC
SGSFAINMLTDEQHGIARLFSTPGTTPEQRYTEGEWHTLVTGSPILKGAVAAFDCVVEEC
CCCEEEEEECCCCCHHHHHHCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
IQSGTHHLYIGRVLAAEYLDQNILLYRDGLFRKLERII
HHCCCCEEEHHHHHHHHHHCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA