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The map label for this gene is hcaA [C]

Identifier: 50084880

GI number: 50084880

Start: 1735408

End: 1736241

Strand: Reverse

Name: hcaA [C]

Synonym: ACIAD1726

Alternate gene names: 50084880

Gene position: 1736241-1735408 (Counterclockwise)

Preceding gene: 50084883

Following gene: 50084879

Centisome position: 48.25

GC content: 44.48

Gene sequence:

>834_bases
ATGACATACGATAAACGTTGGGAAACCGTTGATGTACAAGTAGAACATGGAATTGCATGGGTCACACTTAATCGTCCTCA
CAAAAAAAATGCAATGAGCCCAACACTCAACCGTGAAATGATCGATGTGCTTGAAACACTCGAGCTAGATTCAGAGGCAA
AAGTGCTGGTGTTAACTGGTGCCGGTGATTCTTGGACTGCTGGCATGGATTTAAAAGAATATTTCCGTGAAGTAGATGCT
CAACCTGAAATTTTCCAAGAACGTATTCGACGTGATTCGTGTCGCTGGCAGTGGCAATTACTTCGTATGTACTCTAAACC
AACAATTGCCATGGTTAACGGCTGGTGTTTTGGTGGTGGTTTCTCGCCACTGGTGGCCTGTGATTTAGCCATTGCAGCCG
ATGAAGCCACTTTCGGACTGTCAGAAATTAACTGGGGCATTCCACCGGGCAACCTCGTCAGCAAAGCGATGGCAGATACG
GTTGGGCATCGTGCATCACTGTATTACATTATGACCGGTAAAACTTTTACCGGTAAAGAAGCTGAAGCAATGGGTCTGAT
TAATAAAAGCGTTCCGCTTGCACAGCTAAAGGCAGAAGTGACAGAACTGGCACAGTGTCTGGTCGAGAAAAACCCTGTCG
TTTTACGTACAGCTAAAAATGGCTTTAAGCGCTGTCGTGAGCTGACATGGGATCAGAATGAAGATTATTTATATGCCAAA
CTGGATCAATGTAATCATCGAGATACTGAGGGCGGCCGCCAAGAGGGTTTAAAACAGTTCTTAGATGAAAAATCGATTAA
ACCAGGTTTGCAAAGCTATAAGCGTACAGGTTAA

Upstream 100 bases:

>100_bases
AAAAAAATTATAAAATTCTTCAAAAAACACTTGAATGTAAGTTTAACTGATATTAAATTAAATCCAAGACAGCACAATTT
GTCATTCAAGGAGAAAACAA

Downstream 100 bases:

>100_bases
GTTCGCAACATCAATGCTCAAGTAAGGATACACTCGATGCAACATGTACAGTTACTTATTGATGGTCAGTCTGTCGATGC
TGCCAATCAAGCCACCTTTG

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTYDKRWETVDVQVEHGIAWVTLNRPHKKNAMSPTLNREMIDVLETLELDSEAKVLVLTGAGDSWTAGMDLKEYFREVDA
QPEIFQERIRRDSCRWQWQLLRMYSKPTIAMVNGWCFGGGFSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADT
VGHRASLYYIMTGKTFTGKEAEAMGLINKSVPLAQLKAEVTELAQCLVEKNPVVLRTAKNGFKRCRELTWDQNEDYLYAK
LDQCNHRDTEGGRQEGLKQFLDEKSIKPGLQSYKRTG

Sequences:

>Translated_277_residues
MTYDKRWETVDVQVEHGIAWVTLNRPHKKNAMSPTLNREMIDVLETLELDSEAKVLVLTGAGDSWTAGMDLKEYFREVDA
QPEIFQERIRRDSCRWQWQLLRMYSKPTIAMVNGWCFGGGFSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADT
VGHRASLYYIMTGKTFTGKEAEAMGLINKSVPLAQLKAEVTELAQCLVEKNPVVLRTAKNGFKRCRELTWDQNEDYLYAK
LDQCNHRDTEGGRQEGLKQFLDEKSIKPGLQSYKRTG
>Mature_276_residues
TYDKRWETVDVQVEHGIAWVTLNRPHKKNAMSPTLNREMIDVLETLELDSEAKVLVLTGAGDSWTAGMDLKEYFREVDAQ
PEIFQERIRRDSCRWQWQLLRMYSKPTIAMVNGWCFGGGFSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADTV
GHRASLYYIMTGKTFTGKEAEAMGLINKSVPLAQLKAEVTELAQCLVEKNPVVLRTAKNGFKRCRELTWDQNEDYLYAKL
DQCNHRDTEGGRQEGLKQFLDEKSIKPGLQSYKRTG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=28.7449392712551, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=28.7937743190661, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI70995211, Length=249, Percent_Identity=24.8995983935743, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI45643119, Length=229, Percent_Identity=29.6943231441048, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260275230, Length=229, Percent_Identity=29.6943231441048, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260274832, Length=229, Percent_Identity=29.6943231441048, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI68989263, Length=179, Percent_Identity=27.9329608938547, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=249, Percent_Identity=23.6947791164659, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538581, Length=249, Percent_Identity=23.6947791164659, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI25453481, Length=249, Percent_Identity=23.6947791164659, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538573, Length=249, Percent_Identity=23.6947791164659, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4757968, Length=249, Percent_Identity=24.0963855421687, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI49355787, Length=249, Percent_Identity=24.0963855421687, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI221307494, Length=250, Percent_Identity=23.6, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI221136756, Length=250, Percent_Identity=23.6, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=250, Percent_Identity=23.6, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4502327, Length=279, Percent_Identity=26.8817204301075, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI213417737, Length=241, Percent_Identity=25.7261410788382, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI157694516, Length=241, Percent_Identity=25.7261410788382, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=27.6264591439689, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788597, Length=213, Percent_Identity=33.8028169014084, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI221142681, Length=240, Percent_Identity=29.5833333333333, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=23.9700374531835, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790281, Length=208, Percent_Identity=27.8846153846154, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=26.6009852216749, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=28.8, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=29.6442687747036, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=28.5024154589372, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=25, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=27.6699029126214, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=24.1228070175439, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=204, Percent_Identity=25.9803921568627, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=26.7015706806283, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=273, Percent_Identity=27.1062271062271, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653477, Length=273, Percent_Identity=27.1062271062271, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357171, Length=198, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922422, Length=269, Percent_Identity=25.2788104089219, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=28.0612244897959, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=27.6264591439689, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920382, Length=233, Percent_Identity=25.3218884120172, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583165, Length=278, Percent_Identity=25.5395683453237, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 31331; Mature: 31200

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYDKRWETVDVQVEHGIAWVTLNRPHKKNAMSPTLNREMIDVLETLELDSEAKVLVLTG
CCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEC
AGDSWTAGMDLKEYFREVDAQPEIFQERIRRDSCRWQWQLLRMYSKPTIAMVNGWCFGGG
CCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCC
FSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADTVGHRASLYYIMTGKTFTGKE
CCHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCEECCCC
AEAMGLINKSVPLAQLKAEVTELAQCLVEKNPVVLRTAKNGFKRCRELTWDQNEDYLYAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCEEEEE
LDQCNHRDTEGGRQEGLKQFLDEKSIKPGLQSYKRTG
ECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
TYDKRWETVDVQVEHGIAWVTLNRPHKKNAMSPTLNREMIDVLETLELDSEAKVLVLTG
CCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEC
AGDSWTAGMDLKEYFREVDAQPEIFQERIRRDSCRWQWQLLRMYSKPTIAMVNGWCFGGG
CCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCC
FSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADTVGHRASLYYIMTGKTFTGKE
CCHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCEECCCC
AEAMGLINKSVPLAQLKAEVTELAQCLVEKNPVVLRTAKNGFKRCRELTWDQNEDYLYAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCEEEEE
LDQCNHRDTEGGRQEGLKQFLDEKSIKPGLQSYKRTG
ECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA