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The map label for this gene is hcaE [H]

Identifier: 50084876

GI number: 50084876

Start: 1729419

End: 1730669

Strand: Reverse

Name: hcaE [H]

Synonym: ACIAD1722

Alternate gene names: 50084876

Gene position: 1730669-1729419 (Counterclockwise)

Preceding gene: 50084877

Following gene: 50084875

Centisome position: 48.09

GC content: 41.25

Gene sequence:

>1251_bases
ATGTCAAAATTATGGATGTACTCTGCTGTGATGCTCTCGGGTTCTGTATTTGCTTCAGACTTTATTGATAAAAGTACAGT
CGAACTCACCACACGAAATTTCTATTTCGATCGCGATTATCAAGAAGTTTCTAAATATCCCGCTGCAAAAGACTGGACAC
AAGGCTTTATTTTTAAAGCAAATTCTGGTTATACCGAAGGAACCATTGGTTTTGGGCTCGATGTTTTGGCTACAGCAGGA
TTTAAGCTCGATGCCAGTGCCAAATATGCGGGTACAGGCAATTTACCTCGCGACACGGTCACCAATGAACCCGCCAGCAG
CTACGGCGAAATTGGCCTGACCGGTAAAGCAAAAGTCAGTCAGACCGAATTAAAAGTAGGAACACTCAGACCCATGAATC
CCGTGCTGGTGGCCTCTCCTGCACGCCTACTACCGCAAACCTACCGTGGTATTTCACTGGAATCGAAAGATATTAAGAAT
TTTGAGCTGCAAGGAGCATTTATAGACAAGGTGAATCAGCGCGACTCGACCAATTATGAAAATATCAGGATTTCAGCGGT
GAATGGGCGTTTCAAAAGTGCTGAAACAAATGGTCTTTACTACGCAGGTGGCTATTACAATGTAAGTCCGGCTTTACGTT
TAGGATTATTTTATCTGGATGTTAATAATTTATACGATCAGATGACAACTGGCTTTTTATACAAATGGGACATAAATCCG
CAAACTCGTTTAAGCAGCTATCTACATTACTATCGTAGCCGCGATGAAGGCCAAGCAAAGGCAGGTTTGGTCGACAACGA
TTTATATCATGCGCATTTTGAGCTTAAACGCCAAAATCATAAATTTATTTTTGGTACCTTTCAGCATTTTGGTGAAACCG
CTTTTCCATATTTAACGGGTGGTGAAACAGGACTGCTCATCGATACATGGCCGGGCGAATTTTTAAATCCGAAAGAAAAA
GCATACAGCTTTCGTTATGAGTATGACTTTAAAGATTATGTGCCGGGTTTACGTTTTATGACACGTTATACAATGGGTAG
AAACATTTACGCGCCAAATCTGGGGGGTACCAATCTTAAGGAACGTGAAACTGATTTTGATATTGGCTATACCATACAAA
CAGGTTGGTTAAAAAATCTGGCTCTTCGAGCTCGCTACGCTATTTATGACAACAACATGCTAGCCACAGCCAACATCAAG
CCAGTCAATGAAACCCGTATTAATATCGATTACACATGGAAGTTTAAATAA

Upstream 100 bases:

>100_bases
GGCGCGAGACGTACTCGGAAAATAATTAATATTTTTTAAACCTCATGGTTGGTACGACCAACCCTGTCAAAACAACAAAG
ATGTTTCAGGAAGAAATACA

Downstream 100 bases:

>100_bases
TAACAATAAGCTCCAGATCTTCTGGAGCTTTTTCAATGATGGATCATATCTATGCAAATTTCTCAGACATTACCCCCCAT
TCACCGTTTATTGGGAGGAC

Product: porin

Products: NA

Alternate protein names: Imipenem/basic amino acid-specific outer membrane pore; Outer membrane protein D2 [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSKLWMYSAVMLSGSVFASDFIDKSTVELTTRNFYFDRDYQEVSKYPAAKDWTQGFIFKANSGYTEGTIGFGLDVLATAG
FKLDASAKYAGTGNLPRDTVTNEPASSYGEIGLTGKAKVSQTELKVGTLRPMNPVLVASPARLLPQTYRGISLESKDIKN
FELQGAFIDKVNQRDSTNYENIRISAVNGRFKSAETNGLYYAGGYYNVSPALRLGLFYLDVNNLYDQMTTGFLYKWDINP
QTRLSSYLHYYRSRDEGQAKAGLVDNDLYHAHFELKRQNHKFIFGTFQHFGETAFPYLTGGETGLLIDTWPGEFLNPKEK
AYSFRYEYDFKDYVPGLRFMTRYTMGRNIYAPNLGGTNLKERETDFDIGYTIQTGWLKNLALRARYAIYDNNMLATANIK
PVNETRINIDYTWKFK

Sequences:

>Translated_416_residues
MSKLWMYSAVMLSGSVFASDFIDKSTVELTTRNFYFDRDYQEVSKYPAAKDWTQGFIFKANSGYTEGTIGFGLDVLATAG
FKLDASAKYAGTGNLPRDTVTNEPASSYGEIGLTGKAKVSQTELKVGTLRPMNPVLVASPARLLPQTYRGISLESKDIKN
FELQGAFIDKVNQRDSTNYENIRISAVNGRFKSAETNGLYYAGGYYNVSPALRLGLFYLDVNNLYDQMTTGFLYKWDINP
QTRLSSYLHYYRSRDEGQAKAGLVDNDLYHAHFELKRQNHKFIFGTFQHFGETAFPYLTGGETGLLIDTWPGEFLNPKEK
AYSFRYEYDFKDYVPGLRFMTRYTMGRNIYAPNLGGTNLKERETDFDIGYTIQTGWLKNLALRARYAIYDNNMLATANIK
PVNETRINIDYTWKFK
>Mature_415_residues
SKLWMYSAVMLSGSVFASDFIDKSTVELTTRNFYFDRDYQEVSKYPAAKDWTQGFIFKANSGYTEGTIGFGLDVLATAGF
KLDASAKYAGTGNLPRDTVTNEPASSYGEIGLTGKAKVSQTELKVGTLRPMNPVLVASPARLLPQTYRGISLESKDIKNF
ELQGAFIDKVNQRDSTNYENIRISAVNGRFKSAETNGLYYAGGYYNVSPALRLGLFYLDVNNLYDQMTTGFLYKWDINPQ
TRLSSYLHYYRSRDEGQAKAGLVDNDLYHAHFELKRQNHKFIFGTFQHFGETAFPYLTGGETGLLIDTWPGEFLNPKEKA
YSFRYEYDFKDYVPGLRFMTRYTMGRNIYAPNLGGTNLKERETDFDIGYTIQTGWLKNLALRARYAIYDNNMLATANIKP
VNETRINIDYTWKFK

Specific function: Porin with a specificity for basic amino acids. Also possesses serine protease activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oprD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005318 [H]

Pfam domain/function: PF03573 OprD [H]

EC number: NA

Molecular weight: Translated: 47401; Mature: 47270

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLWMYSAVMLSGSVFASDFIDKSTVELTTRNFYFDRDYQEVSKYPAAKDWTQGFIFKA
CCCHHEEHHHHHCCCHHHHHHCCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCCEEEEE
NSGYTEGTIGFGLDVLATAGFKLDASAKYAGTGNLPRDTVTNEPASSYGEIGLTGKAKVS
CCCCCCCCHHCCHHHHHCCCEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QTELKVGTLRPMNPVLVASPARLLPQTYRGISLESKDIKNFELQGAFIDKVNQRDSTNYE
CEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCC
NIRISAVNGRFKSAETNGLYYAGGYYNVSPALRLGLFYLDVNNLYDQMTTGFLYKWDINP
EEEEEEECCCCCCCCCCCEEEECCEEECCHHHEEEEEEEEHHHHHHHHHCCEEEEEECCH
QTRLSSYLHYYRSRDEGQAKAGLVDNDLYHAHFELKRQNHKFIFGTFQHFGETAFPYLTG
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEHHHCCCCCCCEEEC
GETGLLIDTWPGEFLNPKEKAYSFRYEYDFKDYVPGLRFMTRYTMGRNIYAPNLGGTNLK
CCEEEEEECCCCCCCCCHHHHEEEEEEECHHHHCCHHHHHHHHHCCCEEECCCCCCCCCC
ERETDFDIGYTIQTGWLKNLALRARYAIYDNNMLATANIKPVNETRINIDYTWKFK
CCCCCCCEEEEEECCHHHHHHHEEEEEEEECCEEEEEECCCCCCEEEEEEEEEEEC
>Mature Secondary Structure 
SKLWMYSAVMLSGSVFASDFIDKSTVELTTRNFYFDRDYQEVSKYPAAKDWTQGFIFKA
CCHHEEHHHHHCCCHHHHHHCCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCCEEEEE
NSGYTEGTIGFGLDVLATAGFKLDASAKYAGTGNLPRDTVTNEPASSYGEIGLTGKAKVS
CCCCCCCCHHCCHHHHHCCCEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QTELKVGTLRPMNPVLVASPARLLPQTYRGISLESKDIKNFELQGAFIDKVNQRDSTNYE
CEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCC
NIRISAVNGRFKSAETNGLYYAGGYYNVSPALRLGLFYLDVNNLYDQMTTGFLYKWDINP
EEEEEEECCCCCCCCCCCEEEECCEEECCHHHEEEEEEEEHHHHHHHHHCCEEEEEECCH
QTRLSSYLHYYRSRDEGQAKAGLVDNDLYHAHFELKRQNHKFIFGTFQHFGETAFPYLTG
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEHHHCCCCCCCEEEC
GETGLLIDTWPGEFLNPKEKAYSFRYEYDFKDYVPGLRFMTRYTMGRNIYAPNLGGTNLK
CCEEEEEECCCCCCCCCHHHHEEEEEEECHHHHCCHHHHHHHHHCCCEEECCCCCCCCCC
ERETDFDIGYTIQTGWLKNLALRARYAIYDNNMLATANIKPVNETRINIDYTWKFK
CCCCCCCEEEEEECCHHHHHHHEEEEEEEECCEEEEEECCCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339257; 1427017; 10984043; 8012497; 2118530; 8843159; 9636669 [H]