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The map label for this gene is pcaU

Identifier: 50084857

GI number: 50084857

Start: 1708109

End: 1708945

Strand: Reverse

Name: pcaU

Synonym: ACIAD1702

Alternate gene names: 50084857

Gene position: 1708945-1708109 (Counterclockwise)

Preceding gene: 50084871

Following gene: 50084854

Centisome position: 47.49

GC content: 39.07

Gene sequence:

>837_bases
ATGTGGTCAAATATGGACGATAAAAAAGTGAAAGAAGAAAAAATTCTTCACAACTCAACCAACAAAAAAATCATTCGCCA
TGAAGATTTTGTTGCTGGAATTAGTAAAGGCATGGCCATACTGGATAGCTTTGGTACAGATCGTCATCGTCTCAATATTA
CCATGGCCGCAGAAAAAACAGGAATGACTCGAGCCGCTGCACGTCGTCATTTACTTACTCTTGAGTATCTGGGCTATCTA
GAATCAGATGGTCACTACTTTTATCTTACGCCAAAAATTCTAAAATTTTCAGGATCTTATCTTGGTGGCGCCCAGCTCCC
TAAAATTTCACAGCCTTTACTTAATTTACTGACTACCCAAACATCACTGATCTATTCAGTCATGGTACTGGATGGTTATG
AAGCGATTACCATTGCTCGAAGTGCTGCTCATCAACAAACAGATCGTGTAAATCCTTACGGATTACATTTGGGAAATCGT
TTGCCTGCACATGCCACATCAGCTGGCAAAATTCTTTTAGCTTATCTAGATGATCACGCACAGCAAGAATGGCTCAATCA
GTATCCTTTACAACGTTTGACTAAATACACCTATACCAACAACATTGATTTCTTAAGACTTTTATCAGAAATAAAAGAAC
AAGGCTGGTGTTATTCATCAGAAGAACATGAGTTGGGGGTACATGCACTTGCTGTTCCAATTTATGGGCAACAATCACGC
GTGGTTGCAGCATTAAATATCGTCTCTCCAACAATGAGAACGACTAAGGAATATTTGATTCAGCATATTTTGCCATTACT
ACAAGAAACAGCACGTGAATTGCGCAACATTCTTTGA

Upstream 100 bases:

>100_bases
TTAAATAATTTGTTCGATAATCGAACAAAACATGATTTAGTTATAGTCATTCCGCGTTATATTATACATGTCAATATGAC
AATCGTATGTAGTAGCTGGA

Downstream 100 bases:

>100_bases
TTCGAATTTCAAACATAATGTTCGATGATCGCACACAAGTATTTTATTAGAAAATACTCAATCTAGACTTAGATTGAGTA
TTATTGACCATAATATATTC

Product: regulatory protein for pca operon (activator)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MWSNMDDKKVKEEKILHNSTNKKIIRHEDFVAGISKGMAILDSFGTDRHRLNITMAAEKTGMTRAAARRHLLTLEYLGYL
ESDGHYFYLTPKILKFSGSYLGGAQLPKISQPLLNLLTTQTSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNR
LPAHATSAGKILLAYLDDHAQQEWLNQYPLQRLTKYTYTNNIDFLRLLSEIKEQGWCYSSEEHELGVHALAVPIYGQQSR
VVAALNIVSPTMRTTKEYLIQHILPLLQETARELRNIL

Sequences:

>Translated_278_residues
MWSNMDDKKVKEEKILHNSTNKKIIRHEDFVAGISKGMAILDSFGTDRHRLNITMAAEKTGMTRAAARRHLLTLEYLGYL
ESDGHYFYLTPKILKFSGSYLGGAQLPKISQPLLNLLTTQTSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNR
LPAHATSAGKILLAYLDDHAQQEWLNQYPLQRLTKYTYTNNIDFLRLLSEIKEQGWCYSSEEHELGVHALAVPIYGQQSR
VVAALNIVSPTMRTTKEYLIQHILPLLQETARELRNIL
>Mature_278_residues
MWSNMDDKKVKEEKILHNSTNKKIIRHEDFVAGISKGMAILDSFGTDRHRLNITMAAEKTGMTRAAARRHLLTLEYLGYL
ESDGHYFYLTPKILKFSGSYLGGAQLPKISQPLLNLLTTQTSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNR
LPAHATSAGKILLAYLDDHAQQEWLNQYPLQRLTKYTYTNNIDFLRLLSEIKEQGWCYSSEEHELGVHALAVPIYGQQSR
VVAALNIVSPTMRTTKEYLIQHILPLLQETARELRNIL

Specific function: Activates transcription of the pca operon

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain

Homologues:

Organism=Escherichia coli, GI1788131, Length=260, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786468, Length=228, Percent_Identity=27.6315789473684, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786715, Length=200, Percent_Identity=26.5, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082359, Length=235, Percent_Identity=24.2553191489362, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789999, Length=226, Percent_Identity=27.4336283185841, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI145693096, Length=250, Percent_Identity=25.2, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): PCAU_ACIAD (O83046)

Other databases:

- EMBL:   L05770
- EMBL:   CR543861
- RefSeq:   YP_046367.1
- ProteinModelPortal:   O83046
- SMR:   O83046
- STRING:   O83046
- GeneID:   2878292
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1702
- NMPDR:   fig|62977.3.peg.1629
- eggNOG:   COG1414
- HOGENOM:   HBG739407
- OMA:   GQAYVDS
- ProtClustDB:   CLSK927690
- BioCyc:   ASP62977:ACIAD1702-MONOMER
- GO:   GO:0006350
- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00346
- TIGRFAMs:   TIGR02431

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR

EC number: NA

Molecular weight: Translated: 31596; Mature: 31596

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS51077 HTH_ICLR; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSNMDDKKVKEEKILHNSTNKKIIRHEDFVAGISKGMAILDSFGTDRHRLNITMAAEKT
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
GMTRAAARRHLLTLEYLGYLESDGHYFYLTPKILKFSGSYLGGAQLPKISQPLLNLLTTQ
CHHHHHHHHHHHHHHHHHHEECCCCEEEECHHHEEECCCCCCCCCCCHHHHHHHHHHHHH
TSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNRLPAHATSAGKILLAYLDDHA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEEHHHHH
QQEWLNQYPLQRLTKYTYTNNIDFLRLLSEIKEQGWCYSSEEHELGVHALAVPIYGQQSR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCC
VVAALNIVSPTMRTTKEYLIQHILPLLQETARELRNIL
EEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWSNMDDKKVKEEKILHNSTNKKIIRHEDFVAGISKGMAILDSFGTDRHRLNITMAAEKT
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
GMTRAAARRHLLTLEYLGYLESDGHYFYLTPKILKFSGSYLGGAQLPKISQPLLNLLTTQ
CHHHHHHHHHHHHHHHHHHEECCCCEEEECHHHEEECCCCCCCCCCCHHHHHHHHHHHHH
TSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNRLPAHATSAGKILLAYLDDHA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEEHHHHH
QQEWLNQYPLQRLTKYTYTNNIDFLRLLSEIKEQGWCYSSEEHELGVHALAVPIYGQQSR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCC
VVAALNIVSPTMRTTKEYLIQHILPLLQETARELRNIL
EEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515921