BacMap

Definition	Acinetobacter sp. ADP1 chromosome, complete genome.
Accession	NC_005966
Length	3,598,621

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The map label for this gene is dcaE [H]

Identifier: 50084847

GI number: 50084847

Start: 1696924

End: 1697709

Strand: Reverse

Name: dcaE [H]

Synonym: ACIAD1692

Alternate gene names: 50084847

Gene position: 1697709-1696924 (Counterclockwise)

Preceding gene: 50084854

Following gene: 50084846

Centisome position: 47.18

GC content: 42.11

Gene sequence:

>786_bases
ATGATTCCAGATCAGGATAATTTCGTTGAAATTGATTTTTCAATTGAACAAATTGCAATCGTCAAGATTAACCGACCAGC
CTCAAAGAATGCTTTAAATACAGAAGTTCGTAAGCAACTTGCACAAGCATTTACAGAGCTGAGCTTTAATGACCAAATCA
ATGCAATTGTTCTAACGGGTGGTGAGGATGTCTTTGCAGCAGGTGCAGACCTCAAAGAAATGGCAACAGCAAGCTCGACC
GATATGTTGTTACGTCATACCGAACGTTACTGGAATGCTATTGCACAATGTCCCAAGCCCGTAATTGCAGCCGTAAACGG
TTATGCTTTGGGAGGTGGTTGTGAGCTGGCCATGCATACCGACATTATTATTGCAGGAAAAAGCGCTACTTTCGGGCAGC
CTGAAATTAAGGTTGGTTTGATGCCAGGTGCAGGCGGTACGCAACGTTTATTTCGTGCCGTTGGAAAATTTCATGCGATG
CGTATGATCATGACAGGTGTCATGGTTCCAGCAGAAGAAGCCTATCTGATTGGTCTAGTGTCTCAAGTCACTGAAGATAG
TCAAACAATTCCTACCGCAATCAAAATGGCTCAAAGTCTTGCAAAAATGCCACCTATTGCTTTACAGCAAATTAAAGAAG
TCGCTTTGATGAGTGAAGATGTCCCCTTAAATGCCGGCTTAACTCTGGAACGCAAATCGTTTCAATTGCTGTTTTCTACC
GAAGATAAAAATGAAGGTATCAATGCGTTTATCGAAAAACGTAAACCCTCCTATCACGGTAAATAA

Upstream 100 bases:

>100_bases
TCATCATTGTGAATCTATAAAAAACACTTGAAAAAAAATCTAGTTTAAGCATCATATGAGGAATATAGTTTTGTTATGCG
AAACAAAGGATGTAAAAATA

Downstream 100 bases:

>100_bases
AAACATTAAGAACACATATCTAGGAAGAAATGATGGCACGTAAAGTACTGATTACTGCTGGTGGCTCAGGGATTGGCCGA
TGTATTGCAGAAGCTTTTTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIPDQDNFVEIDFSIEQIAIVKINRPASKNALNTEVRKQLAQAFTELSFNDQINAIVLTGGEDVFAAGADLKEMATASST
DMLLRHTERYWNAIAQCPKPVIAAVNGYALGGGCELAMHTDIIIAGKSATFGQPEIKVGLMPGAGGTQRLFRAVGKFHAM
RMIMTGVMVPAEEAYLIGLVSQVTEDSQTIPTAIKMAQSLAKMPPIALQQIKEVALMSEDVPLNAGLTLERKSFQLLFST
EDKNEGINAFIEKRKPSYHGK

Sequences:

>Translated_261_residues
MIPDQDNFVEIDFSIEQIAIVKINRPASKNALNTEVRKQLAQAFTELSFNDQINAIVLTGGEDVFAAGADLKEMATASST
DMLLRHTERYWNAIAQCPKPVIAAVNGYALGGGCELAMHTDIIIAGKSATFGQPEIKVGLMPGAGGTQRLFRAVGKFHAM
RMIMTGVMVPAEEAYLIGLVSQVTEDSQTIPTAIKMAQSLAKMPPIALQQIKEVALMSEDVPLNAGLTLERKSFQLLFST
EDKNEGINAFIEKRKPSYHGK
>Mature_261_residues
MIPDQDNFVEIDFSIEQIAIVKINRPASKNALNTEVRKQLAQAFTELSFNDQINAIVLTGGEDVFAAGADLKEMATASST
DMLLRHTERYWNAIAQCPKPVIAAVNGYALGGGCELAMHTDIIIAGKSATFGQPEIKVGLMPGAGGTQRLFRAVGKFHAM
RMIMTGVMVPAEEAYLIGLVSQVTEDSQTIPTAIKMAQSLAKMPPIALQQIKEVALMSEDVPLNAGLTLERKSFQLLFST
EDKNEGINAFIEKRKPSYHGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=242, Percent_Identity=45.0413223140496, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=37.4501992031873, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=35.8695652173913, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI70995211, Length=251, Percent_Identity=29.8804780876494, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=33.6898395721925, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=29.4820717131474, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI45643119, Length=250, Percent_Identity=29.2, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI260275230, Length=250, Percent_Identity=29.2, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI260274832, Length=250, Percent_Identity=29.2, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI37594471, Length=176, Percent_Identity=25.5681818181818, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI37594469, Length=176, Percent_Identity=25.5681818181818, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI25453481, Length=242, Percent_Identity=26.0330578512397, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538573, Length=242, Percent_Identity=26.0330578512397, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=219, Percent_Identity=28.7671232876712, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=219, Percent_Identity=28.7671232876712, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=23.1441048034935, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=23.1441048034935, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI4757968, Length=242, Percent_Identity=25.6198347107438, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=242, Percent_Identity=25.6198347107438, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=218, Percent_Identity=26.1467889908257, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136753, Length=218, Percent_Identity=26.1467889908257, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221307494, Length=218, Percent_Identity=26.1467889908257, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI62530384, Length=248, Percent_Identity=24.1935483870968, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI261878539, Length=96, Percent_Identity=39.5833333333333, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI157694520, Length=199, Percent_Identity=22.1105527638191, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=246, Percent_Identity=44.3089430894309, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=36.6533864541833, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=32.5670498084291, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=31.1284046692607, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788682, Length=195, Percent_Identity=33.8461538461538, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=33.5106382978723, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=44.7154471544715, Blast_Score=214, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=42.3868312757202, Blast_Score=198, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17540714, Length=236, Percent_Identity=33.0508474576271, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=30.2040816326531, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=26.7441860465116, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17558304, Length=206, Percent_Identity=31.5533980582524, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=175, Percent_Identity=35.4285714285714, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=175, Percent_Identity=35.4285714285714, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=175, Percent_Identity=35.4285714285714, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=226, Percent_Identity=30.5309734513274, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=214, Percent_Identity=28.0373831775701, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=236, Percent_Identity=30.0847457627119, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=206, Percent_Identity=24.2718446601942, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=206, Percent_Identity=24.2718446601942, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=199, Percent_Identity=28.1407035175879, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=171, Percent_Identity=30.4093567251462, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=184, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=45.2674897119342, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=45.2674897119342, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=32.5581395348837, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21357171, Length=268, Percent_Identity=29.8507462686567, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24650670, Length=217, Percent_Identity=30.4147465437788, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=32.7102803738318, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=28.9795918367347, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24654903, Length=202, Percent_Identity=24.7524752475248, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571729, Length=173, Percent_Identity=26.5895953757225, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=173, Percent_Identity=26.5895953757225, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921000, Length=173, Percent_Identity=32.9479768786127, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583077, Length=173, Percent_Identity=32.9479768786127, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583079, Length=173, Percent_Identity=32.9479768786127, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583165, Length=241, Percent_Identity=23.2365145228216, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28355; Mature: 28355

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDQDNFVEIDFSIEQIAIVKINRPASKNALNTEVRKQLAQAFTELSFNDQINAIVLTG
CCCCCCCEEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
GEDVFAAGADLKEMATASSTDMLLRHTERYWNAIAQCPKPVIAAVNGYALGGGCELAMHT
CCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCEEECCCEEEEEEE
DIIIAGKSATFGQPEIKVGLMPGAGGTQRLFRAVGKFHAMRMIMTGVMVPAEEAYLIGLV
EEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SQVTEDSQTIPTAIKMAQSLAKMPPIALQQIKEVALMSEDVPLNAGLTLERKSFQLLFST
HHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEC
EDKNEGINAFIEKRKPSYHGK
CCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIPDQDNFVEIDFSIEQIAIVKINRPASKNALNTEVRKQLAQAFTELSFNDQINAIVLTG
CCCCCCCEEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
GEDVFAAGADLKEMATASSTDMLLRHTERYWNAIAQCPKPVIAAVNGYALGGGCELAMHT
CCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCEEECCCEEEEEEE
DIIIAGKSATFGQPEIKVGLMPGAGGTQRLFRAVGKFHAMRMIMTGVMVPAEEAYLIGLV
EEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SQVTEDSQTIPTAIKMAQSLAKMPPIALQQIKEVALMSEDVPLNAGLTLERKSFQLLFST
HHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEC
EDKNEGINAFIEKRKPSYHGK
CCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]