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The map label for this gene is ywbI [H]

Identifier: 50084832

GI number: 50084832

Start: 1680830

End: 1681729

Strand: Direct

Name: ywbI [H]

Synonym: ACIAD1675

Alternate gene names: 50084832

Gene position: 1680830-1681729 (Clockwise)

Preceding gene: 50084829

Following gene: 50084833

Centisome position: 46.71

GC content: 38.22

Gene sequence:

>900_bases
ATGGATATTAAATCGCTTAAAATTTTTGTACAAATTATTCAGAGTCAAAGTTTTACTTTGGCAGCAGAACAGCTTTTTGT
GACACAGCCAACAGTGAGTAAAGCCATTGCGGGTTTAGAAGAAGAATTGGGAACAACATTATTTAAAAAAGGGGAAGCTG
GCCGAAAACGTGAAGTCGAACTGACTTACACAGGTCAACAAGTTTATCAGCATGCGCTAAACATACTTGATGAACAAAAG
AAAATTTATGAAGTACTGGATGATATTAAACATCTGAAAAGAGGGAAGTTGACCTTAGGTTTGCCGCCGCTTGGTTCAGT
TTTATTAACATCACTTATAGCAATTTTTCATAAGCAACATCCGAATATTGAACTCAAGTTTCTTGAGGTCGGTTCAAATA
CGATTGAGCAAGCGATTCTGGCGAAAACCATTGATGTTGGAATATTACTCAATCATATACATCCGCTTTTGGCTGTCATT
CCAATTATTGATTCGCCGTTATGTTTATTATCACCCAGACATTCGCGCTGGAAGCATGCCAAAGGCATAACGCTGAACGA
TTTAAAAGATGAACAATTTTTACTTTATGATGATAGTTTTACACTTAATCATTTGATTATACAGGCGGCCAATCTGGAGG
GCTTTGAACCTCATATTGTGTGTAAAAGCAGTCAATGGGACTTTATTGCCAAGCTCGTCGAGTCTGCTATGGGTATTGCT
TTATTACCACAGATTTATTGTGAACAGCTCGATCAAGATAAATTTTCGATTACCCCTCTGATCAATACGCCGTTACGATG
GACTTTAAGTATGGCATGGAATACTTCTGTTCCGATGACAGCCGCAACACGAGCATGGCTGGCCATCGTAGAACAGTATC
GGCATGAAATTAAATTTTAA

Upstream 100 bases:

>100_bases
TTAAGCTGAGTGATTTTATAGGGAATAAATGTATTTTAGATCGACTTTTATGTCATATTATGAATAATAAAATTATAATT
ATTCTTTTATGGAATATTTT

Downstream 100 bases:

>100_bases
CAAATAAAAAATTGAATAAAATACAAATTTATATATTATAAAAATATATAATGTTTATGGGCGAACATATGCATGACTTC
TTATTGGCGTTCTTAGGTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDIKSLKIFVQIIQSQSFTLAAEQLFVTQPTVSKAIAGLEEELGTTLFKKGEAGRKREVELTYTGQQVYQHALNILDEQK
KIYEVLDDIKHLKRGKLTLGLPPLGSVLLTSLIAIFHKQHPNIELKFLEVGSNTIEQAILAKTIDVGILLNHIHPLLAVI
PIIDSPLCLLSPRHSRWKHAKGITLNDLKDEQFLLYDDSFTLNHLIIQAANLEGFEPHIVCKSSQWDFIAKLVESAMGIA
LLPQIYCEQLDQDKFSITPLINTPLRWTLSMAWNTSVPMTAATRAWLAIVEQYRHEIKF

Sequences:

>Translated_299_residues
MDIKSLKIFVQIIQSQSFTLAAEQLFVTQPTVSKAIAGLEEELGTTLFKKGEAGRKREVELTYTGQQVYQHALNILDEQK
KIYEVLDDIKHLKRGKLTLGLPPLGSVLLTSLIAIFHKQHPNIELKFLEVGSNTIEQAILAKTIDVGILLNHIHPLLAVI
PIIDSPLCLLSPRHSRWKHAKGITLNDLKDEQFLLYDDSFTLNHLIIQAANLEGFEPHIVCKSSQWDFIAKLVESAMGIA
LLPQIYCEQLDQDKFSITPLINTPLRWTLSMAWNTSVPMTAATRAWLAIVEQYRHEIKF
>Mature_299_residues
MDIKSLKIFVQIIQSQSFTLAAEQLFVTQPTVSKAIAGLEEELGTTLFKKGEAGRKREVELTYTGQQVYQHALNILDEQK
KIYEVLDDIKHLKRGKLTLGLPPLGSVLLTSLIAIFHKQHPNIELKFLEVGSNTIEQAILAKTIDVGILLNHIHPLLAVI
PIIDSPLCLLSPRHSRWKHAKGITLNDLKDEQFLLYDDSFTLNHLIIQAANLEGFEPHIVCKSSQWDFIAKLVESAMGIA
LLPQIYCEQLDQDKFSITPLINTPLRWTLSMAWNTSVPMTAATRAWLAIVEQYRHEIKF

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=289, Percent_Identity=28.3737024221453, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=27.3648648648649, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790399, Length=284, Percent_Identity=24.2957746478873, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693105, Length=327, Percent_Identity=24.7706422018349, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=24.1106719367589, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=239, Percent_Identity=26.7782426778243, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367136, Length=253, Percent_Identity=22.5296442687747, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=130, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=22.4409448818898, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=253, Percent_Identity=22.5296442687747, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789440, Length=149, Percent_Identity=30.8724832214765, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081904, Length=147, Percent_Identity=29.9319727891156, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=25.2032520325203, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=222, Percent_Identity=28.3783783783784, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=302, Percent_Identity=23.841059602649, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33808; Mature: 33808

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKSLKIFVQIIQSQSFTLAAEQLFVTQPTVSKAIAGLEEELGTTLFKKGEAGRKREVE
CCHHHHHHHHHHHHCCCCEEEHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LTYTGQQVYQHALNILDEQKKIYEVLDDIKHLKRGKLTLGLPPLGSVLLTSLIAIFHKQH
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCC
PNIELKFLEVGSNTIEQAILAKTIDVGILLNHIHPLLAVIPIIDSPLCLLSPRHSRWKHA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHC
KGITLNDLKDEQFLLYDDSFTLNHLIIQAANLEGFEPHIVCKSSQWDFIAKLVESAMGIA
CCCEECCCCCCEEEEEECCCCHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCHH
LLPQIYCEQLDQDKFSITPLINTPLRWTLSMAWNTSVPMTAATRAWLAIVEQYRHEIKF
HHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIKSLKIFVQIIQSQSFTLAAEQLFVTQPTVSKAIAGLEEELGTTLFKKGEAGRKREVE
CCHHHHHHHHHHHHCCCCEEEHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LTYTGQQVYQHALNILDEQKKIYEVLDDIKHLKRGKLTLGLPPLGSVLLTSLIAIFHKQH
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCC
PNIELKFLEVGSNTIEQAILAKTIDVGILLNHIHPLLAVIPIIDSPLCLLSPRHSRWKHA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHC
KGITLNDLKDEQFLLYDDSFTLNHLIIQAANLEGFEPHIVCKSSQWDFIAKLVESAMGIA
CCCEECCCCCCEEEEEECCCCHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCHH
LLPQIYCEQLDQDKFSITPLINTPLRWTLSMAWNTSVPMTAATRAWLAIVEQYRHEIKF
HHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]