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The map label for this gene is cysK [H]

Identifier: 50084819

GI number: 50084819

Start: 1666966

End: 1667964

Strand: Direct

Name: cysK [H]

Synonym: ACIAD1662

Alternate gene names: 50084819

Gene position: 1666966-1667964 (Clockwise)

Preceding gene: 50084818

Following gene: 50084827

Centisome position: 46.32

GC content: 41.34

Gene sequence:

>999_bases
ATGTCTACCGATCAAGAGTTTAAAGCACCAGCAAATTTGGGCATAGCAGTATATTCAAATAATGCAGAAGCAATTGGAAA
TACGCCTTTAGTACGCATTAATCGTATTATTAAAACTGGTGCAACGGTTTTAGGTAAAGTTGAGAGTCGAAACCCTGCAT
TCTCAGTAAAATGCCGCATTGGTGCTGCTTTAATTGCAGATGCAGAGAAACGTGGTGTATTAAAATCTGGTATGCATATT
GTTGAACCGACTAGTGGCAATACAGGGATTGCTTTAGCTTTTGTTGCAGCTGCAAAAGGTTACCCGATTACCTTAACCAT
GCCAGCAAGTATGAGTCTTGAGCGCCGAAAAGTGCTTAAAGCTTTCGGTGCAAACTTGGTTTTGACAGAACCAGCAAAAG
GAATGAAAGGTGCAGTGGAGGAAGCAGTACGATTGGCGACAGAACAGCCAGATATATATTTCCTGCCACAACAGTTCGAA
AATCCAGCTAACCCTAAAATTCACGAACAAACCACAGGGCCAGAAATTTGGGATGCAACCAATGGGCAAGTTGATATTTT
AGTCGCTGGAGTTGGTACAGGCGGCACAATTTCAGGTATTTCGCATTATTTTGAAAAAGTTCAGAATAAACCCATTTATT
CAGTTGCAGTTGAACCTGCAGAATCTCCGATTATCACTCAGACCAAGCGTGGTGAGGAGATTACACCTGCACCTCATAAA
ATCCAAGGTATTGGCGCCAATTTTATTCCAAAGAATCTGGACTTAGATATTGTAGATGAAGTCATGCCCATCACTAGTGA
TGAAGCATTGGAATGGGCTCGTAAATGTGCGACTCAAGAAGGAATTTTGGGGGGTATTTCATGTGGTGCAGCCTTAGCTG
CTGCTGCAAAAATTGCAGAACGACCTGAAAATGAAGGTAAAACTATTGTTGTGATTTTACCAGATAGCGGTGAACGCTAC
TTATCTTCTGTATTATTTGAAGGTATGTTTGAGGAATAG

Upstream 100 bases:

>100_bases
TACAGATTGGAGAGAAGGAGAACTTTGGTAGTTTATTTGACAAGATTATTATGATTCAGAATAATGATTTGGAATCATTC
CAAATCTAATAAGGATTAAG

Downstream 100 bases:

>100_bases
AAAAAATATATTCACTCATGTAACAGCATGCCTAGTCTAAAATCTTTTATAGGTATGCTGCAATACATCTGAGCAAGTAT
TAAAATAGCCTAAGCTAATC

Product: subunit of cysteine synthase A and O-acetylserine sulfhydrolase A, PLP-dependent enzyme

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSTDQEFKAPANLGIAVYSNNAEAIGNTPLVRINRIIKTGATVLGKVESRNPAFSVKCRIGAALIADAEKRGVLKSGMHI
VEPTSGNTGIALAFVAAAKGYPITLTMPASMSLERRKVLKAFGANLVLTEPAKGMKGAVEEAVRLATEQPDIYFLPQQFE
NPANPKIHEQTTGPEIWDATNGQVDILVAGVGTGGTISGISHYFEKVQNKPIYSVAVEPAESPIITQTKRGEEITPAPHK
IQGIGANFIPKNLDLDIVDEVMPITSDEALEWARKCATQEGILGGISCGAALAAAAKIAERPENEGKTIVVILPDSGERY
LSSVLFEGMFEE

Sequences:

>Translated_332_residues
MSTDQEFKAPANLGIAVYSNNAEAIGNTPLVRINRIIKTGATVLGKVESRNPAFSVKCRIGAALIADAEKRGVLKSGMHI
VEPTSGNTGIALAFVAAAKGYPITLTMPASMSLERRKVLKAFGANLVLTEPAKGMKGAVEEAVRLATEQPDIYFLPQQFE
NPANPKIHEQTTGPEIWDATNGQVDILVAGVGTGGTISGISHYFEKVQNKPIYSVAVEPAESPIITQTKRGEEITPAPHK
IQGIGANFIPKNLDLDIVDEVMPITSDEALEWARKCATQEGILGGISCGAALAAAAKIAERPENEGKTIVVILPDSGERY
LSSVLFEGMFEE
>Mature_331_residues
STDQEFKAPANLGIAVYSNNAEAIGNTPLVRINRIIKTGATVLGKVESRNPAFSVKCRIGAALIADAEKRGVLKSGMHIV
EPTSGNTGIALAFVAAAKGYPITLTMPASMSLERRKVLKAFGANLVLTEPAKGMKGAVEEAVRLATEQPDIYFLPQQFEN
PANPKIHEQTTGPEIWDATNGQVDILVAGVGTGGTISGISHYFEKVQNKPIYSVAVEPAESPIITQTKRGEEITPAPHKI
QGIGANFIPKNLDLDIVDEVMPITSDEALEWARKCATQEGILGGISCGAALAAAAKIAERPENEGKTIVVILPDSGERYL
SSVLFEGMFEE

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=41.747572815534, Blast_Score=198, Evalue=7e-51,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=41.747572815534, Blast_Score=198, Evalue=7e-51,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=41.747572815534, Blast_Score=198, Evalue=7e-51,
Organism=Escherichia coli, GI1788754, Length=317, Percent_Identity=62.7760252365931, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=40.655737704918, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17535051, Length=306, Percent_Identity=49.6732026143791, Blast_Score=280, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=54.4585987261147, Blast_Score=280, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI17562970, Length=315, Percent_Identity=44.7619047619048, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI32566674, Length=304, Percent_Identity=46.0526315789474, Blast_Score=260, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17561720, Length=315, Percent_Identity=44.1269841269841, Blast_Score=237, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=45.1327433628319, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=36.5930599369085, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25147552, Length=330, Percent_Identity=32.7272727272727, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17561716, Length=129, Percent_Identity=41.0852713178295, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=320, Percent_Identity=36.25, Blast_Score=165, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6321449, Length=330, Percent_Identity=33.9393939393939, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24643623, Length=318, Percent_Identity=35.8490566037736, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI20129101, Length=318, Percent_Identity=35.8490566037736, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 35306; Mature: 35175

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDQEFKAPANLGIAVYSNNAEAIGNTPLVRINRIIKTGATVLGKVESRNPAFSVKCRI
CCCCCCCCCCCCCEEEEECCCCHHCCCCCHHHHHHHHHHCHHHEEEECCCCCCEEEEEEE
GAALIADAEKRGVLKSGMHIVEPTSGNTGIALAFVAAAKGYPITLTMPASMSLERRKVLK
CEEEEECCHHCCHHHCCCEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHH
AFGANLVLTEPAKGMKGAVEEAVRLATEQPDIYFLPQQFENPANPKIHEQTTGPEIWDAT
HHCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCC
NGQVDILVAGVGTGGTISGISHYFEKVQNKPIYSVAVEPAESPIITQTKRGEEITPAPHK
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCE
IQGIGANFIPKNLDLDIVDEVMPITSDEALEWARKCATQEGILGGISCGAALAAAAKIAE
EEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHH
RPENEGKTIVVILPDSGERYLSSVLFEGMFEE
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STDQEFKAPANLGIAVYSNNAEAIGNTPLVRINRIIKTGATVLGKVESRNPAFSVKCRI
CCCCCCCCCCCCEEEEECCCCHHCCCCCHHHHHHHHHHCHHHEEEECCCCCCEEEEEEE
GAALIADAEKRGVLKSGMHIVEPTSGNTGIALAFVAAAKGYPITLTMPASMSLERRKVLK
CEEEEECCHHCCHHHCCCEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHH
AFGANLVLTEPAKGMKGAVEEAVRLATEQPDIYFLPQQFENPANPKIHEQTTGPEIWDAT
HHCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCC
NGQVDILVAGVGTGGTISGISHYFEKVQNKPIYSVAVEPAESPIITQTKRGEEITPAPHK
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCE
IQGIGANFIPKNLDLDIVDEVMPITSDEALEWARKCATQEGILGGISCGAALAAAAKIAE
EEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHH
RPENEGKTIVVILPDSGERYLSSVLFEGMFEE
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7603442 [H]