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The map label for this gene is glpE [C]

Identifier: 50084747

GI number: 50084747

Start: 1577735

End: 1578229

Strand: Reverse

Name: glpE [C]

Synonym: ACIAD1585

Alternate gene names: 50084747

Gene position: 1578229-1577735 (Counterclockwise)

Preceding gene: 50084759

Following gene: 50084746

Centisome position: 43.86

GC content: 40.2

Gene sequence:

>495_bases
ATGAATGCCAAAATTAATACATCAGACCATTTAAAATCTGCAGAAAAGATATCGTCGGAGCATATATTCCAACAAGCACA
ACAATACGCAGAAGATCATGATCTTAATTTCACTGGTAGTGTTTCTCCTGTAGATGCATGGCATTTAATTCAACAGGGAC
AAGCAGTTCTGGTCGATGTTCGTACTAATGAAGAACGTAAGTTTGTCGGCTATGTTCCAGAAAGTATTCACATTGCCTGG
GCGACAGGTACCGCTTTTAATCGGAATCCACGTTTTTTAAAAGAATTGGAGAATAAAGTCGGTAAAGATAAAACGATCCT
GCTACTTTGCCGTAGTGGTAAACGTTCAGCGCTTGCAGCAGAAGCCGCCTTTAATGCGGGCTTTGAGCAAATTTATAACA
TTTTGGAAGGATTTGAAGGTGATTTAAATCAACAGCAGCAGCGTAATAGCCAAAATGGTTGGCGTATTCACCAACTCCCA
TGGCAACAGGATTAA

Upstream 100 bases:

>100_bases
TATTTTATATATAGAAATTATATTTAATTATTTTTAAGAATAAAGAATTGGGCATAGCATATTCTTATATTGAAATGATT
TAGTTTTAAGGATGCTTTTC

Downstream 100 bases:

>100_bases
CTCAACCTTTATATCGCAAATCATTTAAATCACTTCATTAAGGAATAAATGTGGCTAAATGGGATATTTCTCCTGTTGTA
CAAGGTTTACAACAGGCACG

Product: rhodanese-like sulfurtransferase

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNAKINTSDHLKSAEKISSEHIFQQAQQYAEDHDLNFTGSVSPVDAWHLIQQGQAVLVDVRTNEERKFVGYVPESIHIAW
ATGTAFNRNPRFLKELENKVGKDKTILLLCRSGKRSALAAEAAFNAGFEQIYNILEGFEGDLNQQQQRNSQNGWRIHQLP
WQQD

Sequences:

>Translated_164_residues
MNAKINTSDHLKSAEKISSEHIFQQAQQYAEDHDLNFTGSVSPVDAWHLIQQGQAVLVDVRTNEERKFVGYVPESIHIAW
ATGTAFNRNPRFLKELENKVGKDKTILLLCRSGKRSALAAEAAFNAGFEQIYNILEGFEGDLNQQQQRNSQNGWRIHQLP
WQQD
>Mature_164_residues
MNAKINTSDHLKSAEKISSEHIFQQAQQYAEDHDLNFTGSVSPVDAWHLIQQGQAVLVDVRTNEERKFVGYVPESIHIAW
ATGTAFNRNPRFLKELENKVGKDKTILLLCRSGKRSALAAEAAFNAGFEQIYNILEGFEGDLNQQQQRNSQNGWRIHQLP
WQQD

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 18678; Mature: 18678

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKINTSDHLKSAEKISSEHIFQQAQQYAEDHDLNFTGSVSPVDAWHLIQQGQAVLVDV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEE
RTNEERKFVGYVPESIHIAWATGTAFNRNPRFLKELENKVGKDKTILLLCRSGKRSALAA
ECCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
EAAFNAGFEQIYNILEGFEGDLNQQQQRNSQNGWRIHQLPWQQD
HHHHCCCHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCCCCCC
>Mature Secondary Structure
MNAKINTSDHLKSAEKISSEHIFQQAQQYAEDHDLNFTGSVSPVDAWHLIQQGQAVLVDV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEE
RTNEERKFVGYVPESIHIAWATGTAFNRNPRFLKELENKVGKDKTILLLCRSGKRSALAA
ECCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
EAAFNAGFEQIYNILEGFEGDLNQQQQRNSQNGWRIHQLPWQQD
HHHHCCCHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11319266 [H]