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The map label for this gene is thcD [H]

Identifier: 50084742

GI number: 50084742

Start: 1571442

End: 1572683

Strand: Direct

Name: thcD [H]

Synonym: ACIAD1580

Alternate gene names: 50084742

Gene position: 1571442-1572683 (Clockwise)

Preceding gene: 50084741

Following gene: 50084748

Centisome position: 43.67

GC content: 42.11

Gene sequence:

>1242_bases
ATGTCAAATTACGATGTGATTATCGTGGGCACAGGGCATGCGGGCGGACAGGCGGCTGTCGCACTCAGACAACAAGGATT
TACTGGATCAATTTTGATGATCGGTGAAGAATTACATTTGCCTTATGAGCGACCACCATTATCAAAAGAGTATTTCTCTG
GTGAAAAAACGTTTGAGCGAATTTTGTTGCGTCCAGAACAGTTTTGGAAGGATAAAAATATTGACCTAAAGCTTGGACAA
CGCGTGATTCAGATTGATGCCCAGTCACATCGTATTTTGACCCAGCAAAACCACGAATATCACTATGGAAAATTGATTTG
GGCGACTGGTGGAAAACCACGTCGGCTAAGTTGTGAAGGTGCAGATTTAGACGGGATTCATTATATTCGTAATCGCGAAG
ATGTAGATCGCATTAATCAGGAGCTTGATCGTGTTCAAAAATGTGTGGTCATTGGTGGTGGATATATTGGTTTAGAGGCT
GCCAGTGCATTACGCAAAATTAATCGAGATGTGACTTTGGTTGAAGCACAGTCAAGAGTGCTGGCGCGTGTTGCAGGGCC
CATAATTTCTGATTTTTATCAGCAATATCATCAGCAAAAAGGCATTGAATTTTATTTAGGGCAAGGAGTGGATCATCTTG
AAGGTGATCAGGGGCGTGTGCATACGGTGATACTCGCCAATGGAACACGAATTGCGACGGATATGGTAATTGTTGGGATA
GGGTTAAACCCTGAAATTAATGCTTTGATTGAGGCAGGTGCGATATCGAGTAATGGCATTGAAACGGATCGCCGTTGCCG
AACTTCTCTGCCTGATATTTTTGCAATTGGCGATTGTGCTAATCATGAAAATATTTTTGCCGATGGACAGCGTATCAGAC
TCGAATCTGTACAAAATGCCAATGATCAAGCCATGATTGTCGCCAAAGAAATCTTAGATAAAGGTGAAGACTACGCGGCC
ATACCGTGGTTTTGGTCAAATCAGTATGATCTGAAATTACAAACGATTGGGCTTTCAATTGGCTATGATCAGGAGGTTCT
ACGCAGTGAACCCGACTCAGGCTCATTTACCGTGATTTATTTACGTCAAGGCACAATCATAGCACTGGATTGTGTCAATC
GGCCTGCTGATTTTATACAAGGCAAAGCCATTATTCAGCAATCAATCAGTATTCCAACAGAACAATTAAGTGATCATACA
CTGCCATTTAAAGAGCTGATACAACGCTATAAAAACAGTTAA

Upstream 100 bases:

>100_bases
TAGTAGCTTATCACGCCGCGAATCTTCTCGTTTAGCCTGTCAGGTCATCATTGATGAACAATGTGATGATCTTGTAGTTG
AAATTGCACTGGAGGAATAA

Downstream 100 bases:

>100_bases
TTTACACCCCTGAGACGCATTTACAATTGATTAAGTTAGAGCATTAGGCCGCTCTAACTTATTTTTTTTCGAAACCGTAG
GTTAAAATATCAACCATTTT

Product: ferredoxin reductase component (dioxygenase)

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSNYDVIIVGTGHAGGQAAVALRQQGFTGSILMIGEELHLPYERPPLSKEYFSGEKTFERILLRPEQFWKDKNIDLKLGQ
RVIQIDAQSHRILTQQNHEYHYGKLIWATGGKPRRLSCEGADLDGIHYIRNREDVDRINQELDRVQKCVVIGGGYIGLEA
ASALRKINRDVTLVEAQSRVLARVAGPIISDFYQQYHQQKGIEFYLGQGVDHLEGDQGRVHTVILANGTRIATDMVIVGI
GLNPEINALIEAGAISSNGIETDRRCRTSLPDIFAIGDCANHENIFADGQRIRLESVQNANDQAMIVAKEILDKGEDYAA
IPWFWSNQYDLKLQTIGLSIGYDQEVLRSEPDSGSFTVIYLRQGTIIALDCVNRPADFIQGKAIIQQSISIPTEQLSDHT
LPFKELIQRYKNS

Sequences:

>Translated_413_residues
MSNYDVIIVGTGHAGGQAAVALRQQGFTGSILMIGEELHLPYERPPLSKEYFSGEKTFERILLRPEQFWKDKNIDLKLGQ
RVIQIDAQSHRILTQQNHEYHYGKLIWATGGKPRRLSCEGADLDGIHYIRNREDVDRINQELDRVQKCVVIGGGYIGLEA
ASALRKINRDVTLVEAQSRVLARVAGPIISDFYQQYHQQKGIEFYLGQGVDHLEGDQGRVHTVILANGTRIATDMVIVGI
GLNPEINALIEAGAISSNGIETDRRCRTSLPDIFAIGDCANHENIFADGQRIRLESVQNANDQAMIVAKEILDKGEDYAA
IPWFWSNQYDLKLQTIGLSIGYDQEVLRSEPDSGSFTVIYLRQGTIIALDCVNRPADFIQGKAIIQQSISIPTEQLSDHT
LPFKELIQRYKNS
>Mature_412_residues
SNYDVIIVGTGHAGGQAAVALRQQGFTGSILMIGEELHLPYERPPLSKEYFSGEKTFERILLRPEQFWKDKNIDLKLGQR
VIQIDAQSHRILTQQNHEYHYGKLIWATGGKPRRLSCEGADLDGIHYIRNREDVDRINQELDRVQKCVVIGGGYIGLEAA
SALRKINRDVTLVEAQSRVLARVAGPIISDFYQQYHQQKGIEFYLGQGVDHLEGDQGRVHTVILANGTRIATDMVIVGIG
LNPEINALIEAGAISSNGIETDRRCRTSLPDIFAIGDCANHENIFADGQRIRLESVQNANDQAMIVAKEILDKGEDYAAI
PWFWSNQYDLKLQTIGLSIGYDQEVLRSEPDSGSFTVIYLRQGTIIALDCVNRPADFIQGKAIIQQSISIPTEQLSDHTL
PFKELIQRYKNS

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=374, Percent_Identity=27.807486631016, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI226437568, Length=374, Percent_Identity=27.807486631016, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI21389617, Length=374, Percent_Identity=27.807486631016, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI22202629, Length=356, Percent_Identity=22.752808988764, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4757732, Length=356, Percent_Identity=22.752808988764, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI195927006, Length=174, Percent_Identity=27.0114942528736, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1788892, Length=407, Percent_Identity=32.4324324324324, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789065, Length=312, Percent_Identity=27.5641025641026, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789765, Length=351, Percent_Identity=27.3504273504274, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=403, Percent_Identity=30.5210918114144, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32564386, Length=330, Percent_Identity=26.0606060606061, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639257, Length=396, Percent_Identity=23.7373737373737, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281359715, Length=396, Percent_Identity=23.7373737373737, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281359713, Length=396, Percent_Identity=23.7373737373737, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24639250, Length=396, Percent_Identity=23.7373737373737, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18543267, Length=396, Percent_Identity=23.7373737373737, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24639252, Length=396, Percent_Identity=23.7373737373737, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585130, Length=334, Percent_Identity=25.4491017964072, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24581020, Length=275, Percent_Identity=23.2727272727273, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28573993, Length=275, Percent_Identity=23.2727272727273, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 46244; Mature: 46113

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYDVIIVGTGHAGGQAAVALRQQGFTGSILMIGEELHLPYERPPLSKEYFSGEKTFER
CCCCEEEEEECCCCCCCEEEEEHHCCCCCEEEEEEHHHCCCCCCCCCCHHHHCCHHHHHH
ILLRPEQFWKDKNIDLKLGQRVIQIDAQSHRILTQQNHEYHYGKLIWATGGKPRRLSCEG
HHCCCHHHCCCCCCCHHHCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCEEECCC
ADLDGIHYIRNREDVDRINQELDRVQKCVVIGGGYIGLEAASALRKINRDVTLVEAQSRV
CCCCCCHHHCCCHHHHHHHHHHHHHHEEEEECCCEEHHHHHHHHHHHCCCEEEEEHHHHH
LARVAGPIISDFYQQYHQQKGIEFYLGQGVDHLEGDQGRVHTVILANGTRIATDMVIVGI
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEEE
GLNPEINALIEAGAISSNGIETDRRCRTSLPDIFAIGDCANHENIFADGQRIRLESVQNA
CCCCCHHHEEEECCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCEECCCCEEEEEHHCCC
NDQAMIVAKEILDKGEDYAAIPWFWSNQYDLKLQTIGLSIGYDQEVLRSEPDSGSFTVIY
CCCEEEEEHHHHHCCCCEEEEEEEECCCEEEEEEEEEEECCCCHHHHHCCCCCCCEEEEE
LRQGTIIALDCVNRPADFIQGKAIIQQSISIPTEQLSDHTLPFKELIQRYKNS
EECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SNYDVIIVGTGHAGGQAAVALRQQGFTGSILMIGEELHLPYERPPLSKEYFSGEKTFER
CCCEEEEEECCCCCCCEEEEEHHCCCCCEEEEEEHHHCCCCCCCCCCHHHHCCHHHHHH
ILLRPEQFWKDKNIDLKLGQRVIQIDAQSHRILTQQNHEYHYGKLIWATGGKPRRLSCEG
HHCCCHHHCCCCCCCHHHCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCEEECCC
ADLDGIHYIRNREDVDRINQELDRVQKCVVIGGGYIGLEAASALRKINRDVTLVEAQSRV
CCCCCCHHHCCCHHHHHHHHHHHHHHEEEEECCCEEHHHHHHHHHHHCCCEEEEEHHHHH
LARVAGPIISDFYQQYHQQKGIEFYLGQGVDHLEGDQGRVHTVILANGTRIATDMVIVGI
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEEE
GLNPEINALIEAGAISSNGIETDRRCRTSLPDIFAIGDCANHENIFADGQRIRLESVQNA
CCCCCHHHEEEECCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCEECCCCEEEEEHHCCC
NDQAMIVAKEILDKGEDYAAIPWFWSNQYDLKLQTIGLSIGYDQEVLRSEPDSGSFTVIY
CCCEEEEEHHHHHCCCCEEEEEEEECCCEEEEEEEEEEECCCCHHHHHCCCCCCCEEEEE
LRQGTIIALDCVNRPADFIQGKAIIQQSISIPTEQLSDHTLPFKELIQRYKNS
EECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]