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The map label for this gene is ntaB [H]

Identifier: 50084723

GI number: 50084723

Start: 1554408

End: 1555382

Strand: Direct

Name: ntaB [H]

Synonym: ACIAD1561

Alternate gene names: 50084723

Gene position: 1554408-1555382 (Clockwise)

Preceding gene: 50084722

Following gene: 50084724

Centisome position: 43.19

GC content: 39.59

Gene sequence:

>975_bases
TTGAAGTATACAAGGATAGTGAACATGAATATGATGACTCAAGATCAGTCAATTGAAGTATTAGATCCGAAAGCATTCCG
TAGAGCATTGGGCAACTTTGCAACAGGTGTGACGATCATGACTGCGCAAAATGCTGAAGGAGAACGAGTAGGAGTGACTG
CAAATAGTTTCAACTCTGTCTCGCTGGATCCTGCACTTATTTTATGGAGCATCGATAAGAATTCTTCGAGTTTTAAAGTA
TTTGAACAAGCGACACATTTTTCTGTCAATGTTTTGTCGGGAAATCAAATTGATTTATCGAACAAGTTCGCACGACGCAG
TGAAGATAAATTTGCAGGAACTGATTTTGACCATGGTGAAGGGCAATGCATCTTATTAAAACAATGTGCAGCGAATTTTG
AATGCGAAAAATTTAATATCGTTGAAGGTGGTGATCACTGGATTATTATTGGACGTGTGGTCAGATTTTATGATCATGGT
CGCTCGCCTTTGCTTTACCATCAAGGAGCATATTCTTCGGTTTTACCACATCCAAGTTTGGATCGAAAAGAAATTGCTGC
TGGTGAAGGCGATGGTTTCCCTGGTCGTCTCTATCACAACATGTATTATCTACTGACTCAGGCAGTACGTGCTTATCAAG
CAAACTATCATCCGAAGCAACTCGCAAGTGGTTTTCGTACGAGTGAAGCTCGATTATTACTCGTACTCGAAAGCAAAACT
GCAACTTCAAAATGTGAGTTACAGCGTGAAGTGGCTATGCCTGTGGTTGAAATTGAACAAGCAACTGAAATTTTAGCGCA
AAAAGGTTTACTTTTTGATACTGGCGAATGTTACGAATTGACAGAGCAGGGCATTGAAGCTGCTCAGATGCTATATCGGA
TTGCTGAAAATCATCAGCAAGAGATCTTTGAGAAATATTCAACTGATGAACGTGAGCTTTTTAAAAGTATGCTAAAAGAT
CTGATTGGAATTTGA

Upstream 100 bases:

>100_bases
TCACATGACTGGCGCACATGCTTATACAGACTATGATGTATGTGCACAAATTTTAGGTCGTGCTGTAATGGGCATGGAAG
CTGACCCTACAATGGTTTAA

Downstream 100 bases:

>100_bases
TCGCATTAGAATCTATATAAATATAGAATTTATTTTCACCTCAGGCAACTGTGTTTTATGAGGTGAAAATTATAGATAAA
GCCTCTTTCATAAATCAGAA

Product: nitrilotriacetate monooxygenase component B (NtaB)

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKYTRIVNMNMMTQDQSIEVLDPKAFRRALGNFATGVTIMTAQNAEGERVGVTANSFNSVSLDPALILWSIDKNSSSFKV
FEQATHFSVNVLSGNQIDLSNKFARRSEDKFAGTDFDHGEGQCILLKQCAANFECEKFNIVEGGDHWIIIGRVVRFYDHG
RSPLLYHQGAYSSVLPHPSLDRKEIAAGEGDGFPGRLYHNMYYLLTQAVRAYQANYHPKQLASGFRTSEARLLLVLESKT
ATSKCELQREVAMPVVEIEQATEILAQKGLLFDTGECYELTEQGIEAAQMLYRIAENHQQEIFEKYSTDERELFKSMLKD
LIGI

Sequences:

>Translated_324_residues
MKYTRIVNMNMMTQDQSIEVLDPKAFRRALGNFATGVTIMTAQNAEGERVGVTANSFNSVSLDPALILWSIDKNSSSFKV
FEQATHFSVNVLSGNQIDLSNKFARRSEDKFAGTDFDHGEGQCILLKQCAANFECEKFNIVEGGDHWIIIGRVVRFYDHG
RSPLLYHQGAYSSVLPHPSLDRKEIAAGEGDGFPGRLYHNMYYLLTQAVRAYQANYHPKQLASGFRTSEARLLLVLESKT
ATSKCELQREVAMPVVEIEQATEILAQKGLLFDTGECYELTEQGIEAAQMLYRIAENHQQEIFEKYSTDERELFKSMLKD
LIGI
>Mature_324_residues
MKYTRIVNMNMMTQDQSIEVLDPKAFRRALGNFATGVTIMTAQNAEGERVGVTANSFNSVSLDPALILWSIDKNSSSFKV
FEQATHFSVNVLSGNQIDLSNKFARRSEDKFAGTDFDHGEGQCILLKQCAANFECEKFNIVEGGDHWIIIGRVVRFYDHG
RSPLLYHQGAYSSVLPHPSLDRKEIAAGEGDGFPGRLYHNMYYLLTQAVRAYQANYHPKQLASGFRTSEARLLLVLESKT
ATSKCELQREVAMPVVEIEQATEILAQKGLLFDTGECYELTEQGIEAAQMLYRIAENHQQEIFEKYSTDERELFKSMLKD
LIGI

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=144, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 36532; Mature: 36532

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTRIVNMNMMTQDQSIEVLDPKAFRRALGNFATGVTIMTAQNAEGERVGVTANSFNSV
CCCEEEEEEEEECCCCCCEEECHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCC
SLDPALILWSIDKNSSSFKVFEQATHFSVNVLSGNQIDLSNKFARRSEDKFAGTDFDHGE
CCCCEEEEEEECCCCCCHHHEECCCEEEEEEECCCEECCCHHHHHCCCCCCCCCCCCCCC
GQCILLKQCAANFECEKFNIVEGGDHWIIIGRVVRFYDHGRSPLLYHQGAYSSVLPHPSL
CCEEEEEHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCEEEECCCCHHCCCCCCC
DRKEIAAGEGDGFPGRLYHNMYYLLTQAVRAYQANYHPKQLASGFRTSEARLLLVLESKT
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEECCC
ATSKCELQREVAMPVVEIEQATEILAQKGLLFDTGECYELTEQGIEAAQMLYRIAENHQQ
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EIFEKYSTDERELFKSMLKDLIGI
HHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYTRIVNMNMMTQDQSIEVLDPKAFRRALGNFATGVTIMTAQNAEGERVGVTANSFNSV
CCCEEEEEEEEECCCCCCEEECHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCC
SLDPALILWSIDKNSSSFKVFEQATHFSVNVLSGNQIDLSNKFARRSEDKFAGTDFDHGE
CCCCEEEEEEECCCCCCHHHEECCCEEEEEEECCCEECCCHHHHHCCCCCCCCCCCCCCC
GQCILLKQCAANFECEKFNIVEGGDHWIIIGRVVRFYDHGRSPLLYHQGAYSSVLPHPSL
CCEEEEEHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCEEEECCCCHHCCCCCCC
DRKEIAAGEGDGFPGRLYHNMYYLLTQAVRAYQANYHPKQLASGFRTSEARLLLVLESKT
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEECCC
ATSKCELQREVAMPVVEIEQATEILAQKGLLFDTGECYELTEQGIEAAQMLYRIAENHQQ
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EIFEKYSTDERELFKSMLKDLIGI
HHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]