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The map label for this gene is flbD [H]

Identifier: 50084685

GI number: 50084685

Start: 1519714

End: 1520790

Strand: Direct

Name: flbD [H]

Synonym: ACIAD1519

Alternate gene names: 50084685

Gene position: 1519714-1520790 (Clockwise)

Preceding gene: 50084683

Following gene: 50084693

Centisome position: 42.23

GC content: 35.93

Gene sequence:

>1077_bases
ATGAAAACCTTTTTAGATAATGATTATAAAGTAATTACTTTGCCTGATAATGGTGTTCCTGTAAAACCAATAAGAGCAAA
AGCAATTATTTTTTCAGATCAGAACTCTCGTAAATTACTAAATCAAGTTGAAACGATTGCTCCAAGCTCTGCTAGTGTTA
TTATTCAAGGAGAGTCAGGAACAGGTAAAGAATTAATTGCACGCGAAATCCATTTGAAAAGTGATCGTAAAAATAAGCCT
TTTCTTGCAGTCAATTGTGGTGCCTTGGTCGAGACGCTTGCTGAATCTGAACTGTTTGGCTATGAAGCTGGTGCTTTTAC
GGGTGCTAATAAAACTCGTGCTGGTATTTTTGAAACAGCCGATGGTGGTACTTTATTTTTAGATGAAGTAGGTGAATTAC
CTCTAACTTTACAAGTTAAGCTGTTAAGAGTCTTGCAAGAAAAAGAGGTCGTACGCGTGGGTGGTCGAAAACCAATTCCA
ATTAATGTTCGTATTTTGTCGGGCACACATGTCGATCTACGTAAAGCTGTAGCAGAAAATAAGTTTCGTCTAGACTTATA
TTATCGCTTAAACGTAGTTGTGCTTAATATTTTACCATTACGTGAACGTAAAGGAGATATTTTACCTCTAGTTGATTTCT
TCTTAAGTTCATACTCTCAGAAGCTTATTACCACTAAACCTAACATCAGTGATAGAGCAAGAAATGCGCTGCTGAACTAT
AGCTGGCCAGGCAACATTCGTGAGCTAGAAAACAATATTCACTATGCTTTACTGGTGAGTGGCGGTAAAACAATTGATCT
TGAGCATTTAAGATTTTCTGAGGAAACGGGTTCTGCCGTGTTACCAGACAATGATCTTATTCAAGAGTTTGAAAGTATTT
TGAAGCGTTTGTTTCAAGAATATGATGGGGAAGCATGGCATATTATTGAAGATAAAATAGTAAATACTGCATTTTCAAGT
TGCTGGAATAATCAGGTCAAGACAGCAAAAATGTTAGGCCTATCGCGTAATGTTTTACGTAATCTTCTATCAAGATATAA
CTTACTACCTAATAAGAATGAAAATGAAATTGCTTAA

Upstream 100 bases:

>100_bases
ATTTGGGATTAAATATATTTATTTGTTATTTATTAAAAAACCCTATGAAATGTAATATAGATTTTTAAAATTTTTGTATC
ATAAGTTTTATGGGGTAATT

Downstream 100 bases:

>100_bases
GGGTTTTACATAGAAAAAACATCCATTTTTTAATGGATGTTTTTTTGCGTTGTGAATTTTGTGACCCATAAACCCAATTC
AAATAACAACCACATTGGGA

Product: sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKTFLDNDYKVITLPDNGVPVKPIRAKAIIFSDQNSRKLLNQVETIAPSSASVIIQGESGTGKELIAREIHLKSDRKNKP
FLAVNCGALVETLAESELFGYEAGAFTGANKTRAGIFETADGGTLFLDEVGELPLTLQVKLLRVLQEKEVVRVGGRKPIP
INVRILSGTHVDLRKAVAENKFRLDLYYRLNVVVLNILPLRERKGDILPLVDFFLSSYSQKLITTKPNISDRARNALLNY
SWPGNIRELENNIHYALLVSGGKTIDLEHLRFSEETGSAVLPDNDLIQEFESILKRLFQEYDGEAWHIIEDKIVNTAFSS
CWNNQVKTAKMLGLSRNVLRNLLSRYNLLPNKNENEIA

Sequences:

>Translated_358_residues
MKTFLDNDYKVITLPDNGVPVKPIRAKAIIFSDQNSRKLLNQVETIAPSSASVIIQGESGTGKELIAREIHLKSDRKNKP
FLAVNCGALVETLAESELFGYEAGAFTGANKTRAGIFETADGGTLFLDEVGELPLTLQVKLLRVLQEKEVVRVGGRKPIP
INVRILSGTHVDLRKAVAENKFRLDLYYRLNVVVLNILPLRERKGDILPLVDFFLSSYSQKLITTKPNISDRARNALLNY
SWPGNIRELENNIHYALLVSGGKTIDLEHLRFSEETGSAVLPDNDLIQEFESILKRLFQEYDGEAWHIIEDKIVNTAFSS
CWNNQVKTAKMLGLSRNVLRNLLSRYNLLPNKNENEIA
>Mature_358_residues
MKTFLDNDYKVITLPDNGVPVKPIRAKAIIFSDQNSRKLLNQVETIAPSSASVIIQGESGTGKELIAREIHLKSDRKNKP
FLAVNCGALVETLAESELFGYEAGAFTGANKTRAGIFETADGGTLFLDEVGELPLTLQVKLLRVLQEKEVVRVGGRKPIP
INVRILSGTHVDLRKAVAENKFRLDLYYRLNVVVLNILPLRERKGDILPLVDFFLSSYSQKLITTKPNISDRARNALLNY
SWPGNIRELENNIHYALLVSGGKTIDLEHLRFSEETGSAVLPDNDLIQEFESILKRLFQEYDGEAWHIIEDKIVNTAFSS
CWNNQVKTAKMLGLSRNVLRNLLSRYNLLPNKNENEIA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=309, Percent_Identity=42.0711974110032, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI1788550, Length=313, Percent_Identity=44.7284345047923, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082117, Length=240, Percent_Identity=49.5833333333333, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=40.6153846153846, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1786524, Length=235, Percent_Identity=51.063829787234, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1790299, Length=309, Percent_Identity=41.4239482200647, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1788905, Length=316, Percent_Identity=43.3544303797468, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=341, Percent_Identity=39.0029325513196, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=39.2638036809816, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1789087, Length=239, Percent_Identity=48.9539748953975, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1787583, Length=327, Percent_Identity=33.0275229357798, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=36.3636363636364, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI87081858, Length=254, Percent_Identity=37.4015748031496, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40186; Mature: 40186

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFLDNDYKVITLPDNGVPVKPIRAKAIIFSDQNSRKLLNQVETIAPSSASVIIQGESG
CCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCC
TGKELIAREIHLKSDRKNKPFLAVNCGALVETLAESELFGYEAGAFTGANKTRAGIFETA
CCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEC
DGGTLFLDEVGELPLTLQVKLLRVLQEKEVVRVGGRKPIPINVRILSGTHVDLRKAVAEN
CCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCC
KFRLDLYYRLNVVVLNILPLRERKGDILPLVDFFLSSYSQKLITTKPNISDRARNALLNY
CEEEEEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHCEEEC
SWPGNIRELENNIHYALLVSGGKTIDLEHLRFSEETGSAVLPDNDLIQEFESILKRLFQE
CCCCCHHHHHCCCEEEEEECCCCEEEHHHEEECCCCCCEECCCHHHHHHHHHHHHHHHHH
YDGEAWHIIEDKIVNTAFSSCWNNQVKTAKMLGLSRNVLRNLLSRYNLLPNKNENEIA
CCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKTFLDNDYKVITLPDNGVPVKPIRAKAIIFSDQNSRKLLNQVETIAPSSASVIIQGESG
CCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCC
TGKELIAREIHLKSDRKNKPFLAVNCGALVETLAESELFGYEAGAFTGANKTRAGIFETA
CCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEC
DGGTLFLDEVGELPLTLQVKLLRVLQEKEVVRVGGRKPIPINVRILSGTHVDLRKAVAEN
CCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCC
KFRLDLYYRLNVVVLNILPLRERKGDILPLVDFFLSSYSQKLITTKPNISDRARNALLNY
CEEEEEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHCEEEC
SWPGNIRELENNIHYALLVSGGKTIDLEHLRFSEETGSAVLPDNDLIQEFESILKRLFQE
CCCCCHHHHHCCCEEEEEECCCCEEEHHHEEECCCCCCEECCCHHHHHHHHHHHHHHHHH
YDGEAWHIIEDKIVNTAFSSCWNNQVKTAKMLGLSRNVLRNLLSRYNLLPNKNENEIA
CCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]