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The map label for this gene is gsiB [H]

Identifier: 50084674

GI number: 50084674

Start: 1502249

End: 1503823

Strand: Direct

Name: gsiB [H]

Synonym: ACIAD1506

Alternate gene names: 50084674

Gene position: 1502249-1503823 (Clockwise)

Preceding gene: 50084673

Following gene: 50084675

Centisome position: 41.75

GC content: 40.63

Gene sequence:

>1575_bases
ATGAATAAAATTACCCGTAGGGAACTCATTAAACAAACTGGATTAGGTGTTGGTGCTTTTGCTGCAATCAGCCTCACAGG
ATGTTCTAAACCTGATTCTTCATCCTCTGAAAGTACTACTGCCGCTATAACCCAAAGTGATACGCCGAAAAAAGGCGGTG
TGATCAAAATTGGAGTGATCAATGGTAACCAAGCAGGTAATCTCGACGCGCATAAACCAATTGGTATGTCTAGTGCGTTT
AGAGGTTGGCCTTTGTACGCGAAGTTATGGGAATGGGGTAGAGATATTCAGCCAAAACTTGCTCTTGCTGAATTTGCTGA
ACCCAATGATGATGGGACGAAATGGACTATTCGTCTAAAAAAAGGGCTTGAGTTTCATCATGGTAAAACCATTTCAGCTG
ATGATATCATTTTCTCTTTAAGACGGTTAACTGACCCCAAACTTGCCTCACCATTTGCAGCTTATCTTTATTCTTTACAA
AGAGATGCAATTAAAAAGCGTGATGAATACACTGTTGAAATTCCTTTTTCTCAGGGAAAGGGATTGGTTGCTTTACCAGA
AGCGTGGATGAGCTGGGGTGGTATTGTTCCTACAGATTATGATCCAGTTAAAAATGTAGTTGGAGCTGGCCCATTTAAGC
TTGAAAGTTTTACACCTGGTCAGCGTTCACGTTTTATTCGTTTTGAAAATTACTGGAAAGAAAATCAACCTTATGCAGAT
GCAATAGAAATTATTGATTTTAAGGATCAGACAGGGCGCCTTGCTGCTTTGCAATCCGGCCAAATTGATATTGCCTCTGG
CGTAAGTGCTGAGCATATTGCTCTAATTCAGTCTAATAAACGGCTAAATCTAGTTTCATCGGAAACCGATGCTTGGCAAT
CATTTGATATGAATACGTCTAAAGCACCGTTCAATGATGCTCGGGTACGACAAGCTTTTAGATTAATTGCTAATAGAGAC
GATTTAGTTAAAAGGGCTTTAGCTGGACAAGGAAGAGTCGCAAATGATCTTTATTCATTTAGCGATCCTGTATATGACCA
CAATATCGCTCAACGAAAGCAAGATTTGAATAAAGCCAAGCATTTGCTTAAAGAAGCTGGTTTTAATAACAGTTTAAAGG
TCGATTTATATACAGGGCCAGATAGCAATGCCGCATTAGTTTTTGCTCAACATGCTGCTCAGGCAGGTGTCATTGTTAAT
GTTAAACAGGTTGAAGCTGCTACTTTTGCGGATGCTGTAAAACAAGATTGGGCATTTAGTACAGGCTCGAACGTTTCACG
TCCTTTCTTATTAACAGTTTTGCAAATTGATGGGCCGGGTGCGGCAAATAATAAAACTCGATTTAAGAATGAGCGTTTTA
CCGAACTAGTGCATGCGGCATTGCAACAACCCGATTTAGAAAAACGAAAAGTTCTGGTTCACGAAGCACAGCATATTCAA
CATGAGCAAGGTGGTTTACTCATTTGGGGGTTTAATAATGTCCTTGATGCACATTCGAACCAAATTGGTGGTGTAACACC
TGATCGCACTGGTTTCGCTGCATGGCGTACAGATGAATTCTGGCGCAGAGGGTAA

Upstream 100 bases:

>100_bases
TTATTGGAGCTTAATCATGGTTTTGAGTTGCCTAAGCTAAATAGAAATTCATCTATTGATTCCCACTTGAATCAAGAAGT
CGTGGTTTAAGGGAGAAGGT

Downstream 100 bases:

>100_bases
CCGATATGACAATTCAATCCAGCAAAATAGAAGCATCCATTCAACAACCAGCAATTACCAAGTCATTTGAATTTCCTGCA
TGGTTACCTTGGTTTTTGAC

Product: peptide ABC transporter periplasmic substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MNKITRRELIKQTGLGVGAFAAISLTGCSKPDSSSSESTTAAITQSDTPKKGGVIKIGVINGNQAGNLDAHKPIGMSSAF
RGWPLYAKLWEWGRDIQPKLALAEFAEPNDDGTKWTIRLKKGLEFHHGKTISADDIIFSLRRLTDPKLASPFAAYLYSLQ
RDAIKKRDEYTVEIPFSQGKGLVALPEAWMSWGGIVPTDYDPVKNVVGAGPFKLESFTPGQRSRFIRFENYWKENQPYAD
AIEIIDFKDQTGRLAALQSGQIDIASGVSAEHIALIQSNKRLNLVSSETDAWQSFDMNTSKAPFNDARVRQAFRLIANRD
DLVKRALAGQGRVANDLYSFSDPVYDHNIAQRKQDLNKAKHLLKEAGFNNSLKVDLYTGPDSNAALVFAQHAAQAGVIVN
VKQVEAATFADAVKQDWAFSTGSNVSRPFLLTVLQIDGPGAANNKTRFKNERFTELVHAALQQPDLEKRKVLVHEAQHIQ
HEQGGLLIWGFNNVLDAHSNQIGGVTPDRTGFAAWRTDEFWRRG

Sequences:

>Translated_524_residues
MNKITRRELIKQTGLGVGAFAAISLTGCSKPDSSSSESTTAAITQSDTPKKGGVIKIGVINGNQAGNLDAHKPIGMSSAF
RGWPLYAKLWEWGRDIQPKLALAEFAEPNDDGTKWTIRLKKGLEFHHGKTISADDIIFSLRRLTDPKLASPFAAYLYSLQ
RDAIKKRDEYTVEIPFSQGKGLVALPEAWMSWGGIVPTDYDPVKNVVGAGPFKLESFTPGQRSRFIRFENYWKENQPYAD
AIEIIDFKDQTGRLAALQSGQIDIASGVSAEHIALIQSNKRLNLVSSETDAWQSFDMNTSKAPFNDARVRQAFRLIANRD
DLVKRALAGQGRVANDLYSFSDPVYDHNIAQRKQDLNKAKHLLKEAGFNNSLKVDLYTGPDSNAALVFAQHAAQAGVIVN
VKQVEAATFADAVKQDWAFSTGSNVSRPFLLTVLQIDGPGAANNKTRFKNERFTELVHAALQQPDLEKRKVLVHEAQHIQ
HEQGGLLIWGFNNVLDAHSNQIGGVTPDRTGFAAWRTDEFWRRG
>Mature_524_residues
MNKITRRELIKQTGLGVGAFAAISLTGCSKPDSSSSESTTAAITQSDTPKKGGVIKIGVINGNQAGNLDAHKPIGMSSAF
RGWPLYAKLWEWGRDIQPKLALAEFAEPNDDGTKWTIRLKKGLEFHHGKTISADDIIFSLRRLTDPKLASPFAAYLYSLQ
RDAIKKRDEYTVEIPFSQGKGLVALPEAWMSWGGIVPTDYDPVKNVVGAGPFKLESFTPGQRSRFIRFENYWKENQPYAD
AIEIIDFKDQTGRLAALQSGQIDIASGVSAEHIALIQSNKRLNLVSSETDAWQSFDMNTSKAPFNDARVRQAFRLIANRD
DLVKRALAGQGRVANDLYSFSDPVYDHNIAQRKQDLNKAKHLLKEAGFNNSLKVDLYTGPDSNAALVFAQHAAQAGVIVN
VKQVEAATFADAVKQDWAFSTGSNVSRPFLLTVLQIDGPGAANNKTRFKNERFTELVHAALQQPDLEKRKVLVHEAQHIQ
HEQGGLLIWGFNNVLDAHSNQIGGVTPDRTGFAAWRTDEFWRRG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=463, Percent_Identity=26.9978401727862, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789966, Length=473, Percent_Identity=25.5813953488372, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787495, Length=467, Percent_Identity=25.6959314775161, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789397, Length=456, Percent_Identity=22.8070175438596, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787551, Length=520, Percent_Identity=22.5, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787762, Length=418, Percent_Identity=23.2057416267943, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789887, Length=276, Percent_Identity=26.0869565217391, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57975; Mature: 57975

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKITRRELIKQTGLGVGAFAAISLTGCSKPDSSSSESTTAAITQSDTPKKGGVIKIGVI
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEE
NGNQAGNLDAHKPIGMSSAFRGWPLYAKLWEWGRDIQPKLALAEFAEPNDDGTKWTIRLK
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEEE
KGLEFHHGKTISADDIIFSLRRLTDPKLASPFAAYLYSLQRDAIKKRDEYTVEIPFSQGK
CCCEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
GLVALPEAWMSWGGIVPTDYDPVKNVVGAGPFKLESFTPGQRSRFIRFENYWKENQPYAD
CEEEECHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHCEEEEHHHCCCCCCHHH
AIEIIDFKDQTGRLAALQSGQIDIASGVSAEHIALIQSNKRLNLVSSETDAWQSFDMNTS
HEEEEECCCCCCCEEEECCCCEEEECCCCCCEEEEEECCCEEEEEECCCCHHHHCCCCCC
KAPFNDARVRQAFRLIANRDDLVKRALAGQGRVANDLYSFSDPVYDHNIAQRKQDLNKAK
CCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HLLKEAGFNNSLKVDLYTGPDSNAALVFAQHAAQAGVIVNVKQVEAATFADAVKQDWAFS
HHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCC
TGSNVSRPFLLTVLQIDGPGAANNKTRFKNERFTELVHAALQQPDLEKRKVLVHEAQHIQ
CCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HEQGGLLIWGFNNVLDAHSNQIGGVTPDRTGFAAWRTDEFWRRG
HCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHCC
>Mature Secondary Structure
MNKITRRELIKQTGLGVGAFAAISLTGCSKPDSSSSESTTAAITQSDTPKKGGVIKIGVI
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEE
NGNQAGNLDAHKPIGMSSAFRGWPLYAKLWEWGRDIQPKLALAEFAEPNDDGTKWTIRLK
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEEE
KGLEFHHGKTISADDIIFSLRRLTDPKLASPFAAYLYSLQRDAIKKRDEYTVEIPFSQGK
CCCEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
GLVALPEAWMSWGGIVPTDYDPVKNVVGAGPFKLESFTPGQRSRFIRFENYWKENQPYAD
CEEEECHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHCEEEEHHHCCCCCCHHH
AIEIIDFKDQTGRLAALQSGQIDIASGVSAEHIALIQSNKRLNLVSSETDAWQSFDMNTS
HEEEEECCCCCCCEEEECCCCEEEECCCCCCEEEEEECCCEEEEEECCCCHHHHCCCCCC
KAPFNDARVRQAFRLIANRDDLVKRALAGQGRVANDLYSFSDPVYDHNIAQRKQDLNKAK
CCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HLLKEAGFNNSLKVDLYTGPDSNAALVFAQHAAQAGVIVNVKQVEAATFADAVKQDWAFS
HHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCC
TGSNVSRPFLLTVLQIDGPGAANNKTRFKNERFTELVHAALQQPDLEKRKVLVHEAQHIQ
CCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HEQGGLLIWGFNNVLDAHSNQIGGVTPDRTGFAAWRTDEFWRRG
HCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA