Click here to switch to the map view.

The map label for this gene is wcaF [C]

Identifier: 50084662

GI number: 50084662

Start: 1489853

End: 1490410

Strand: Direct

Name: wcaF [C]

Synonym: ACIAD1491

Alternate gene names: 50084662

Gene position: 1489853-1490410 (Clockwise)

Preceding gene: 50084661

Following gene: 50084664

Centisome position: 41.4

GC content: 39.43

Gene sequence:

>558_bases
ATGCCTTGGTTGTATTGGAGCCTAAAACCGAAACATAAAGTATGGGCAGAGGAGTGGCAAAAAGAGTATCAACGGTATTT
AATGGATATGGAAACTGTTGAGATCGGCAAGAATTGTTTTATTTCTCCGTTGGCACATATTTTTGCAGAACCTGGGCGAA
AAATCATTATTGGTGATCATAGCTTTATTGCGGCCGATTCAGTTTTACATGGCCCGATTGATATTGGATCTGAAGTTGCG
ATCAATCATCACTGCATTTTAGATGGTGGAAAAGCTGGAATTAAAATTGCAAATCAAGCAAGAATTGCAGCTTATTGTCA
CTTATACGCATTTGATCATGGAATGCAGATGGATAAGCCAATTTATCAGCAAACAGTTCGATCACAAGGAATTTCAGTGG
GAAAAGATGTATGGTTAGGTGCACATGTTGGGGTTAAAGACGGTGTCAAAATTGATGATCATGCTGTGATAGGTATGAAT
AGCATGGTGACTAAAGATGTGATGCAGAATCAGATAGTGGCCGGTAATCCAGCACACTTCATTCGTATGAGAGATTAG

Upstream 100 bases:

>100_bases
TTAACTCGCGGTCGTGAAAAACTCAAGCGATTAGAGAGTGAACATGATGAATGACCCTTTGCTCAAATATAGAGAACAAC
ACAAACATCGATTGAATTAC

Downstream 100 bases:

>100_bases
AAAGACCTTGATTTAGAATCAAGATCTTTCTGATGGATTTATTTGAGTATAATTATACTGGAGAAACAGTATGATTAAAC
AACTCATCAAGTGCATTAAA

Product: acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MPWLYWSLKPKHKVWAEEWQKEYQRYLMDMETVEIGKNCFISPLAHIFAEPGRKIIIGDHSFIAADSVLHGPIDIGSEVA
INHHCILDGGKAGIKIANQARIAAYCHLYAFDHGMQMDKPIYQQTVRSQGISVGKDVWLGAHVGVKDGVKIDDHAVIGMN
SMVTKDVMQNQIVAGNPAHFIRMRD

Sequences:

>Translated_185_residues
MPWLYWSLKPKHKVWAEEWQKEYQRYLMDMETVEIGKNCFISPLAHIFAEPGRKIIIGDHSFIAADSVLHGPIDIGSEVA
INHHCILDGGKAGIKIANQARIAAYCHLYAFDHGMQMDKPIYQQTVRSQGISVGKDVWLGAHVGVKDGVKIDDHAVIGMN
SMVTKDVMQNQIVAGNPAHFIRMRD
>Mature_184_residues
PWLYWSLKPKHKVWAEEWQKEYQRYLMDMETVEIGKNCFISPLAHIFAEPGRKIIIGDHSFIAADSVLHGPIDIGSEVAI
NHHCILDGGKAGIKIANQARIAAYCHLYAFDHGMQMDKPIYQQTVRSQGISVGKDVWLGAHVGVKDGVKIDDHAVIGMNS
MVTKDVMQNQIVAGNPAHFIRMRD

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=183, Percent_Identity=27.3224043715847, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 20790; Mature: 20659

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWLYWSLKPKHKVWAEEWQKEYQRYLMDMETVEIGKNCFISPLAHIFAEPGRKIIIGDH
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECC
SFIAADSVLHGPIDIGSEVAINHHCILDGGKAGIKIANQARIAAYCHLYAFDHGMQMDKP
CEEEECCEEECCCCCCCEEEECEEEEEECCCCCEEECCCCEEEHEEEEEEECCCCCCCCH
IYQQTVRSQGISVGKDVWLGAHVGVKDGVKIDDHAVIGMNSMVTKDVMQNQIVAGNPAHF
HHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCEEEECCHHHHHHHHHHCCEECCCCEEE
IRMRD
EEECC
>Mature Secondary Structure 
PWLYWSLKPKHKVWAEEWQKEYQRYLMDMETVEIGKNCFISPLAHIFAEPGRKIIIGDH
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECC
SFIAADSVLHGPIDIGSEVAINHHCILDGGKAGIKIANQARIAAYCHLYAFDHGMQMDKP
CEEEECCEEECCCCCCCEEEECEEEEEECCCCCEEECCCCEEEHEEEEEEECCCCCCCCH
IYQQTVRSQGISVGKDVWLGAHVGVKDGVKIDDHAVIGMNSMVTKDVMQNQIVAGNPAHF
HHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCEEEECCHHHHHHHHHHCCEECCCCEEE
IRMRD
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]