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The map label for this gene is alkM

Identifier: 50084588

GI number: 50084588

Start: 1404733

End: 1405959

Strand: Direct

Name: alkM

Synonym: ACIAD1411

Alternate gene names: 50084588

Gene position: 1404733-1405959 (Clockwise)

Preceding gene: 50084586

Following gene: 50084593

Centisome position: 39.04

GC content: 39.85

Gene sequence:

>1227_bases
ATGAATGCACCTGTACATGTCGATCAAAACTTTGAAGAAGTGATCAATGCTGCAAGATCAATGCGTGAAATTGATCGTAA
ACGTTACTTATGGATGATTAGTCCTGCATTACCAGTCATTGGGATCGGGATTTTGGCAGGTTATCAGTTTTCACCCCGTC
CAATCAAAAAAATATTTGCGCTAGGTGGCCCTATTGTTCTACACATTATTATTCCGGTGATTGATACGATTATTGGAAAA
GATGCCAGCAATCCAACGTCTGAAGAAATCAAGCAGCTTGAAAATGATCCTTATTACGCACGTTTGGTGAAAAGCTTCAT
TCCATTACAGTATATTGCCAATGTTTATGCCTGTTATTTGGTGAGCCGTAAAAAGACATCTTTTATTGATAAAATTCTTT
TAGGCATTTCGATGGGTGCAATCAATGGTATTGCAGTGAATACCGCGCATGAATTGAGCCATAAAGCAGATCGACTCGAT
CATATCCTGTCTCATTTGGCGCTCGTTCCTACAGGATACAATCACTTTCGTATAGAACATCCTTACGGACATCACAAGCG
TGCAGCGACTCCTGAAGATCCAGCTTCGTCGCAAATGGGTGAAACATTTTATGAGTTTTGGCCAAGAACGGTCTTTGGTT
CATTAAAATCTGCAATCGAAATTGAGACACATCGTCTGAAACGTAAAGGCAAAAAGTTTTGGTCAAAAGATAATGAACTA
TTACAGGGCTGGGGGATGAGTGCTGCTTTTCATAGTTCGATAATTGCGATATTTGGTAAAGGAACGATTCCTTATCTGGT
TACGCAAGCTTTTTACGGGATTAGTTTATTTGAGATCATTAATTATATCGAGCATTATGGTCTAAAACGCCAGAAAAGAG
CGGATGGCAATTACGAACGTACCATGCCAGAGCATAGCTGGAATAATAACAATATCGTGACGAATCTGTTTTTATACCAG
TTACAACGACATTCAGATCATCACGCTTATCCGACGCGTCCATTTCAGGCATTGCGTCATTTTGATGAAGCACCTGAGTT
ACCAAGTGGTTATGCCAGTATGTTGTTGCCTGCTATGATTCCGCCACTTTGGTTTAAAATGATGGATAAACGAGTATTTG
AGCATTATAAAGAGGATTTGACCAAAGCCAATATCTATCCAAAACGTCGTGCCAAGATATTGGCCAAGTTTGGTTTGACT
GACCCGAATATAGAAAACGGCAAGTAA

Upstream 100 bases:

>100_bases
GACTTTGACATAGGTTTTTTGGACGATCACAACTGTTTTTAATATATAAGATGGATGATTATGAGCAGTATGAATAGAGG
ATAACAAGAAAGGATTCACT

Downstream 100 bases:

>100_bases
TCAGACCTTATTTTAAAAGAGAGTCTAAGTGACTCTCTTTTTATTAGATTAAGCCATTATTTCAGTTCGCTAGATGACTT
TCCGAGTTCACGACGTGCAA

Product: terminal alkane-1-monooxygenase

Products: NA

Alternate protein names: Alkane hydroxylase; AHs; Terminal alkane hydroxylase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNAPVHVDQNFEEVINAARSMREIDRKRYLWMISPALPVIGIGILAGYQFSPRPIKKIFALGGPIVLHIIIPVIDTIIGK
DASNPTSEEIKQLENDPYYARLVKSFIPLQYIANVYACYLVSRKKTSFIDKILLGISMGAINGIAVNTAHELSHKADRLD
HILSHLALVPTGYNHFRIEHPYGHHKRAATPEDPASSQMGETFYEFWPRTVFGSLKSAIEIETHRLKRKGKKFWSKDNEL
LQGWGMSAAFHSSIIAIFGKGTIPYLVTQAFYGISLFEIINYIEHYGLKRQKRADGNYERTMPEHSWNNNNIVTNLFLYQ
LQRHSDHHAYPTRPFQALRHFDEAPELPSGYASMLLPAMIPPLWFKMMDKRVFEHYKEDLTKANIYPKRRAKILAKFGLT
DPNIENGK

Sequences:

>Translated_408_residues
MNAPVHVDQNFEEVINAARSMREIDRKRYLWMISPALPVIGIGILAGYQFSPRPIKKIFALGGPIVLHIIIPVIDTIIGK
DASNPTSEEIKQLENDPYYARLVKSFIPLQYIANVYACYLVSRKKTSFIDKILLGISMGAINGIAVNTAHELSHKADRLD
HILSHLALVPTGYNHFRIEHPYGHHKRAATPEDPASSQMGETFYEFWPRTVFGSLKSAIEIETHRLKRKGKKFWSKDNEL
LQGWGMSAAFHSSIIAIFGKGTIPYLVTQAFYGISLFEIINYIEHYGLKRQKRADGNYERTMPEHSWNNNNIVTNLFLYQ
LQRHSDHHAYPTRPFQALRHFDEAPELPSGYASMLLPAMIPPLWFKMMDKRVFEHYKEDLTKANIYPKRRAKILAKFGLT
DPNIENGK
>Mature_408_residues
MNAPVHVDQNFEEVINAARSMREIDRKRYLWMISPALPVIGIGILAGYQFSPRPIKKIFALGGPIVLHIIIPVIDTIIGK
DASNPTSEEIKQLENDPYYARLVKSFIPLQYIANVYACYLVSRKKTSFIDKILLGISMGAINGIAVNTAHELSHKADRLD
HILSHLALVPTGYNHFRIEHPYGHHKRAATPEDPASSQMGETFYEFWPRTVFGSLKSAIEIETHRLKRKGKKFWSKDNEL
LQGWGMSAAFHSSIIAIFGKGTIPYLVTQAFYGISLFEIINYIEHYGLKRQKRADGNYERTMPEHSWNNNNIVTNLFLYQ
LQRHSDHHAYPTRPFQALRHFDEAPELPSGYASMLLPAMIPPLWFKMMDKRVFEHYKEDLTKANIYPKRRAKILAKFGLT
DPNIENGK

Specific function: Catalyzes the hydroxylation of n-alkanes in the presence of a NADH-rubredoxin reductase and rubredoxin. It preferably hydroxylases long-chain-length alkanes with at least 12 carbon atoms

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fatty acid desaturase family. AlkB subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALKB_ACIAD (O31250)

Other databases:

- EMBL:   AJ002316
- EMBL:   CR543861
- RefSeq:   YP_046098.1
- STRING:   O31250
- GeneID:   2880706
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1411
- NMPDR:   fig|62977.3.peg.771
- eggNOG:   NOG11338
- HOGENOM:   HBG289367
- OMA:   AMTVVVW
- ProtClustDB:   CLSK927657
- BioCyc:   ASP62977:ACIAD1411-MONOMER
- InterPro:   IPR005804

Pfam domain/function: PF00487 FA_desaturase

EC number: =1.14.15.3

Molecular weight: Translated: 46760; Mature: 46760

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16c93528)-; HASH(0x18087ce0)-; HASH(0x16eae204)-; HASH(0x17e673c0)-; HASH(0x17f0274c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVHVDQNFEEVINAARSMREIDRKRYLWMISPALPVIGIGILAGYQFSPRPIKKIFA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHH
LGGPIVLHIIIPVIDTIIGKDASNPTSEEIKQLENDPYYARLVKSFIPLQYIANVYACYL
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VSRKKTSFIDKILLGISMGAINGIAVNTAHELSHKADRLDHILSHLALVPTGYNHFRIEH
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PYGHHKRAATPEDPASSQMGETFYEFWPRTVFGSLKSAIEIETHRLKRKGKKFWSKDNEL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQGWGMSAAFHSSIIAIFGKGTIPYLVTQAFYGISLFEIINYIEHYGLKRQKRADGNYER
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHH
TMPEHSWNNNNIVTNLFLYQLQRHSDHHAYPTRPFQALRHFDEAPELPSGYASMLLPAMI
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
PPLWFKMMDKRVFEHYKEDLTKANIYPKRRAKILAKFGLTDPNIENGK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure
MNAPVHVDQNFEEVINAARSMREIDRKRYLWMISPALPVIGIGILAGYQFSPRPIKKIFA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHH
LGGPIVLHIIIPVIDTIIGKDASNPTSEEIKQLENDPYYARLVKSFIPLQYIANVYACYL
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VSRKKTSFIDKILLGISMGAINGIAVNTAHELSHKADRLDHILSHLALVPTGYNHFRIEH
HHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PYGHHKRAATPEDPASSQMGETFYEFWPRTVFGSLKSAIEIETHRLKRKGKKFWSKDNEL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQGWGMSAAFHSSIIAIFGKGTIPYLVTQAFYGISLFEIINYIEHYGLKRQKRADGNYER
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHH
TMPEHSWNNNNIVTNLFLYQLQRHSDHHAYPTRPFQALRHFDEAPELPSGYASMLLPAMI
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
PPLWFKMMDKRVFEHYKEDLTKANIYPKRRAKILAKFGLTDPNIENGK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9546151