Click here to switch to the map view.

The map label for this gene is hupB [H]

Identifier: 50084585

GI number: 50084585

Start: 1401297

End: 1401569

Strand: Direct

Name: hupB [H]

Synonym: ACIAD1408

Alternate gene names: 50084585

Gene position: 1401297-1401569 (Clockwise)

Preceding gene: 50084584

Following gene: 50084586

Centisome position: 38.94

GC content: 38.83

Gene sequence:

>273_bases
ATGAATAAATCAGAATTAATTGATGCGATTGCAGAGAAAGGGGGATTGTCTAAAACTGATGCTGGTAAGGCATTGGATGC
GACTATTGCTTCAGTTACAGAAGCGCTAAAAAAAGGTGATACTGTAACTTTAGTTGGTTTTGGTACTTTCAGCGTAAAAG
ATCGTGCTGCACGTACTGGCCGTAACCCTAAAACTGGCGAAGAGCTTCAAATTAAAGCAACTAAAGTACCAAGCTTTAAA
GCTGGTAAAGGTTTGAAAGATTCAGTTGCTTAA

Upstream 100 bases:

>100_bases
GTATTGCTTTACCCCCTAAAGCATTGCTATAAAGGCGTCATGGCCTTTTTAAACTTCAGCCTTGGATCTCAAATAAACGA
ATTAAGAGGACGTTATCTTC

Downstream 100 bases:

>100_bases
TCTTTTAAAATAAGTAGACGCGCCATTTTCGGCGCGTTTTTTATTAAAAAGATGGATTACTGAGTGTTTCATACATTCAT
TCGATAGTGTTTTTCAGTTA

Product: DNA-binding protein HU-beta

Products: NA

Alternate protein names: HU-1; NS1 [H]

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKSELIDAIAEKGGLSKTDAGKALDATIASVTEALKKGDTVTLVGFGTFSVKDRAARTGRNPKTGEELQIKATKVPSFK
AGKGLKDSVA

Sequences:

>Translated_90_residues
MNKSELIDAIAEKGGLSKTDAGKALDATIASVTEALKKGDTVTLVGFGTFSVKDRAARTGRNPKTGEELQIKATKVPSFK
AGKGLKDSVA
>Mature_90_residues
MNKSELIDAIAEKGGLSKTDAGKALDATIASVTEALKKGDTVTLVGFGTFSVKDRAARTGRNPKTGEELQIKATKVPSFK
AGKGLKDSVA

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=70.7865168539326, Blast_Score=127, Evalue=2e-31,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=62.9213483146067, Blast_Score=118, Evalue=8e-29,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=40.4494382022472, Blast_Score=77, Evalue=3e-16,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=38.6363636363636, Blast_Score=72, Evalue=8e-15,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9308; Mature: 9308

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSELIDAIAEKGGLSKTDAGKALDATIASVTEALKKGDTVTLVGFGTFSVKDRAARTG
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCC
RNPKTGEELQIKATKVPSFKAGKGLKDSVA
CCCCCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKSELIDAIAEKGGLSKTDAGKALDATIASVTEALKKGDTVTLVGFGTFSVKDRAARTG
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCC
RNPKTGEELQIKATKVPSFKAGKGLKDSVA
CCCCCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]