Click here to switch to the map view.

The map label for this gene is iscA [H]

Identifier: 50084579

GI number: 50084579

Start: 1397221

End: 1397541

Strand: Reverse

Name: iscA [H]

Synonym: ACIAD1402

Alternate gene names: 50084579

Gene position: 1397541-1397221 (Counterclockwise)

Preceding gene: 50084580

Following gene: 50084578

Centisome position: 38.84

GC content: 35.2

Gene sequence:

>321_bases
ATGATTCACTTAACTGAAACTGCTGCAAATCATGTACGCAATTATCTACTTAACCGTGGTAAAGGCGAAGGCATTCGTGT
AGGTGTGAAAACTTCAGGCTGTTCTGGTTTAGCTTATGTACTGGAATTTGTTGATGACATTAATGAGTACGATCAAGTTT
TTGAACAGCATGGTGTAAAAGTATTTGTCGATCCTAAAAGTATAGTGTATCTCGATGGTTTAGAGATGGATTATGTCAAA
AATGGATTAAATGAAGGCTTTGAGTTTAATAATCCAAATAAAAAAGGTGAATGTGGTTGCGGTGAATCATTTACCGTTTA
A

Upstream 100 bases:

>100_bases
CACTGTTCTGTACTTGCAGAAGATGCCATTAAAGCGGCGATTGAAGACTATCGTAACAAACGATCAAAAGCTTAACTAAT
ACTGACCAGATGGAGTTTTC

Downstream 100 bases:

>100_bases
TCAAATTATCCTCCCTTCTTCTGAATTAAAAACAGTGATCTTCACTGTTTTTAATTTATTAATTTACGATGGAATTCGTC
TCCCATGAATCATTTTGAGT

Product: iron-binding protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MIHLTETAANHVRNYLLNRGKGEGIRVGVKTSGCSGLAYVLEFVDDINEYDQVFEQHGVKVFVDPKSIVYLDGLEMDYVK
NGLNEGFEFNNPNKKGECGCGESFTV

Sequences:

>Translated_106_residues
MIHLTETAANHVRNYLLNRGKGEGIRVGVKTSGCSGLAYVLEFVDDINEYDQVFEQHGVKVFVDPKSIVYLDGLEMDYVK
NGLNEGFEFNNPNKKGECGCGESFTV
>Mature_106_residues
MIHLTETAANHVRNYLLNRGKGEGIRVGVKTSGCSGLAYVLEFVDDINEYDQVFEQHGVKVFVDPKSIVYLDGLEMDYVK
NGLNEGFEFNNPNKKGECGCGESFTV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=105, Percent_Identity=44.7619047619048, Blast_Score=102, Evalue=6e-23,
Organism=Homo sapiens, GI160420328, Length=108, Percent_Identity=34.2592592592593, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788877, Length=105, Percent_Identity=63.8095238095238, Blast_Score=139, Evalue=4e-35,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=43.8095238095238, Blast_Score=102, Evalue=4e-24,
Organism=Escherichia coli, GI1786351, Length=106, Percent_Identity=37.7358490566038, Blast_Score=77, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193209025, Length=105, Percent_Identity=42.8571428571429, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25146411, Length=105, Percent_Identity=42.8571428571429, Blast_Score=104, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323001, Length=106, Percent_Identity=43.3962264150943, Blast_Score=100, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24642263, Length=105, Percent_Identity=42.8571428571429, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11780; Mature: 11780

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHLTETAANHVRNYLLNRGKGEGIRVGVKTSGCSGLAYVLEFVDDINEYDQVFEQHGVK
CEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VFVDPKSIVYLDGLEMDYVKNGLNEGFEFNNPNKKGECGCGESFTV
EEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIHLTETAANHVRNYLLNRGKGEGIRVGVKTSGCSGLAYVLEFVDDINEYDQVFEQHGVK
CEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VFVDPKSIVYLDGLEMDYVKNGLNEGFEFNNPNKKGECGCGESFTV
EEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA