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The map label for this gene is rimO

Identifier: 50084549

GI number: 50084549

Start: 1366542

End: 1367885

Strand: Direct

Name: rimO

Synonym: ACIAD1371

Alternate gene names: 50084549

Gene position: 1366542-1367885 (Clockwise)

Preceding gene: 50084543

Following gene: 50084550

Centisome position: 37.97

GC content: 42.63

Gene sequence:

>1344_bases
ATGAAGTCCCCCAAAGTCGGTTTTGTATCTTTAGGTTGTCCTAAGGCATTGGTCGATTCTGAACGAATTTTAACTCAGTT
GCGTACTGAAGGTTATCAAGTTGCATCAGATTACGATGGTGCTGATCTAGTTGTGGTTAATACCTGTGGTTTTATTGAAT
CTGCGGTACAAGAATCATTAGATGCAATCGGCGAAGCCATGAGTGAAAATGGCCGTGTGATTGTGACAGGATGTTTAGGT
AAAGATGAAGATAAAATTCGTCAGATGCATCCGAATGTTTTAAAAGTGACGGGTGCCGCAGCGTACCAAGATGTTATGGA
AGCTGTTCATGAATATGTACCAGCGCCGCCTAGACATAATCCTTTTGTTGATCTGGTTCCTGAACAGGGTATTCGATTGA
CACCGAAGCATTATGCTTATTTGAAGATTTCGGAAGGGTGTAACCATCGCTGTACATTTTGTATTATTCCAAGCATGCGC
GGTGATTTAGTATCACGTCCAGTTGGCTCGGTACTCGAAGAAGCAGCAGCGCTGAAGCGAGCGGGCGTTAAAGAAGTATT
GGTGATTTCTCAGGATACTTCTGCGTATGGGGTGGATACCAAATACAAGCTTGATTTCTGGAATGGTCAGCCAGTAAAAA
CCAAATTCTATGATATGTGTGAGGCATTAGGTCAGCTCGGTATCTGGGTGCGGTTGCATTATGTGTATCCTTATCCGCAT
GTAGATGCTGTGATCGATTTAATGGCTCAAGGCAAAATTCTTCCATACCTTGATATTCCTTTTCAGCATGCCAGTCCGCG
CATCTTAAAATTGATGAAGCGACCAGCCCATAGTGAAAATACATTAGATCGCATTAAGGTTTGGCGTGAAAAATGTCCAA
ATCTTGTGATTCGCTCAACTTTTGTGGTTGGCTTCCCTGGTGAAACAGAAGAAGATTTTCAGATTTTGCTTGAATGGCTC
AAAGAAGCTCAGCTTGATCGTGTAGGTTGCTTTACTTATTCACCTGTAGAGGGTGCAACAGCAAATGACTTGCCAGATCA
TGTGTCTGAAGAGGTTAAGCAAGAACGTTATGAACGTTTTATGCAGGTTCAGCAGGAAATTTCGGCAGCCAGGTTACAAA
AACGTATTGGTCAAACCATGACTGTTCTGGTGGATAGCCTCGAAGATGAGTATCCTGTTGCAGTTGCACGTTCTTATGCA
GATGCGCCAGAAATCGATGGTAATGTATTTGTAGAGGATATAGATAAATCACTTGTACAGCCAGGTCAGTTGCTCGAAGT
AGAAATTACTGATGCTGACGAATATGATTTGTTTGCCAAACTGATTCAGATCAAGACCGCCTAG

Upstream 100 bases:

>100_bases
TTGGCTGTATTTTGGTGCATTATGCAATCCTTATGGAATCCACCTGTTTTTCTTTCCAGAAACATCATACAATACGCGGA
TTCTAGTGGAGTGCAGATTC

Downstream 100 bases:

>100_bases
GCAGTCGTTTTAAACAGAATTCAATTTAAACATCGCATTTTGGAGCGTAATTATGGCTGAAACTAACAGTCCTCGTTATT
CACGAATTTTGTTAAAGCTG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKSPKVGFVSLGCPKALVDSERILTQLRTEGYQVASDYDGADLVVVNTCGFIESAVQESLDAIGEAMSENGRVIVTGCLG
KDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPRHNPFVDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMR
GDLVSRPVGSVLEEAAALKRAGVKEVLVISQDTSAYGVDTKYKLDFWNGQPVKTKFYDMCEALGQLGIWVRLHYVYPYPH
VDAVIDLMAQGKILPYLDIPFQHASPRILKLMKRPAHSENTLDRIKVWREKCPNLVIRSTFVVGFPGETEEDFQILLEWL
KEAQLDRVGCFTYSPVEGATANDLPDHVSEEVKQERYERFMQVQQEISAARLQKRIGQTMTVLVDSLEDEYPVAVARSYA
DAPEIDGNVFVEDIDKSLVQPGQLLEVEITDADEYDLFAKLIQIKTA

Sequences:

>Translated_447_residues
MKSPKVGFVSLGCPKALVDSERILTQLRTEGYQVASDYDGADLVVVNTCGFIESAVQESLDAIGEAMSENGRVIVTGCLG
KDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPRHNPFVDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMR
GDLVSRPVGSVLEEAAALKRAGVKEVLVISQDTSAYGVDTKYKLDFWNGQPVKTKFYDMCEALGQLGIWVRLHYVYPYPH
VDAVIDLMAQGKILPYLDIPFQHASPRILKLMKRPAHSENTLDRIKVWREKCPNLVIRSTFVVGFPGETEEDFQILLEWL
KEAQLDRVGCFTYSPVEGATANDLPDHVSEEVKQERYERFMQVQQEISAARLQKRIGQTMTVLVDSLEDEYPVAVARSYA
DAPEIDGNVFVEDIDKSLVQPGQLLEVEITDADEYDLFAKLIQIKTA
>Mature_447_residues
MKSPKVGFVSLGCPKALVDSERILTQLRTEGYQVASDYDGADLVVVNTCGFIESAVQESLDAIGEAMSENGRVIVTGCLG
KDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPRHNPFVDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMR
GDLVSRPVGSVLEEAAALKRAGVKEVLVISQDTSAYGVDTKYKLDFWNGQPVKTKFYDMCEALGQLGIWVRLHYVYPYPH
VDAVIDLMAQGKILPYLDIPFQHASPRILKLMKRPAHSENTLDRIKVWREKCPNLVIRSTFVVGFPGETEEDFQILLEWL
KEAQLDRVGCFTYSPVEGATANDLPDHVSEEVKQERYERFMQVQQEISAARLQKRIGQTMTVLVDSLEDEYPVAVARSYA
DAPEIDGNVFVEDIDKSLVQPGQLLEVEITDADEYDLFAKLIQIKTA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=484, Percent_Identity=27.0661157024793, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI28872782, Length=486, Percent_Identity=26.9547325102881, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI93277076, Length=414, Percent_Identity=26.3285024154589, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1787057, Length=439, Percent_Identity=68.7927107061503, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=466, Percent_Identity=27.8969957081545, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=477, Percent_Identity=26.4150943396226, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=212, Percent_Identity=30.6603773584906, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356207, Length=422, Percent_Identity=26.7772511848341, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922432, Length=468, Percent_Identity=23.7179487179487, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_ACIAD (Q6FCH4)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046059.1
- ProteinModelPortal:   Q6FCH4
- SMR:   Q6FCH4
- STRING:   Q6FCH4
- GeneID:   2879548
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1371
- NMPDR:   fig|62977.3.peg.603
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q6FCH4
- ProtClustDB:   PRK14862
- BioCyc:   ASP62977:ACIAD1371-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50084; Mature: 50084

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPKVGFVSLGCPKALVDSERILTQLRTEGYQVASDYDGADLVVVNTCGFIESAVQESL
CCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCHHHHHHHHHHH
DAIGEAMSENGRVIVTGCLGKDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPRHN
HHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCCC
PFVDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPVGSVLEEAAALKR
CCEEECCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHHH
AGVKEVLVISQDTSAYGVDTKYKLDFWNGQPVKTKFYDMCEALGQLGIWVRLHYVYPYPH
CCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCC
VDAVIDLMAQGKILPYLDIPFQHASPRILKLMKRPAHSENTLDRIKVWREKCPNLVIRST
HHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEE
FVVGFPGETEEDFQILLEWLKEAQLDRVGCFTYSPVEGATANDLPDHVSEEVKQERYERF
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
MQVQQEISAARLQKRIGQTMTVLVDSLEDEYPVAVARSYADAPEIDGNVFVEDIDKSLVQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEHHHHHHHHCC
PGQLLEVEITDADEYDLFAKLIQIKTA
CCCEEEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSPKVGFVSLGCPKALVDSERILTQLRTEGYQVASDYDGADLVVVNTCGFIESAVQESL
CCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCHHHHHHHHHHH
DAIGEAMSENGRVIVTGCLGKDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPRHN
HHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCCC
PFVDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPVGSVLEEAAALKR
CCEEECCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHHH
AGVKEVLVISQDTSAYGVDTKYKLDFWNGQPVKTKFYDMCEALGQLGIWVRLHYVYPYPH
CCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCC
VDAVIDLMAQGKILPYLDIPFQHASPRILKLMKRPAHSENTLDRIKVWREKCPNLVIRST
HHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEE
FVVGFPGETEEDFQILLEWLKEAQLDRVGCFTYSPVEGATANDLPDHVSEEVKQERYERF
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
MQVQQEISAARLQKRIGQTMTVLVDSLEDEYPVAVARSYADAPEIDGNVFVEDIDKSLVQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEHHHHHHHHCC
PGQLLEVEITDADEYDLFAKLIQIKTA
CCCEEEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA