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The map label for this gene is ilvA [H]

Identifier: 50084536

GI number: 50084536

Start: 1352630

End: 1354168

Strand: Reverse

Name: ilvA [H]

Synonym: ACIAD1357

Alternate gene names: 50084536

Gene position: 1354168-1352630 (Counterclockwise)

Preceding gene: 50084544

Following gene: 50084535

Centisome position: 37.63

GC content: 41.59

Gene sequence:

>1539_bases
ATGCTGTCTCGTTTGGTTCGACAAATTTTACAAGCAACGGTATATGACGTTGCAATCGAAACTCCACTCGAAGCAGCGCC
ACGAATTAGTGAAAAACTAAACAACAATATTCGCTTTAAACGCGAAGACCTTCAACCCGTTTTCTCTTTCAAACTACGCG
GTGCTTATAACCGTATTAGTCAATTACCGAAATCTCAGTTGGAACGTGGCGTTATTACTGCTTCAGCAGGTAACCATGCT
CAAGGTGTGGCTTTATCTGGTCAAAAGCTGGGGATTCGCGCCATTATCGTGATGCCAAAAACAACGCCAGATATTAAGGT
ACAAGCAGTTAAACGTTTGGGTGGCGAAGTGGTTTTGCATGGTGATTCTTTTGATGTTGCCAATAAATACGCGATTCAGC
GTGCGCAAGATGAAGGTATGACTTTTATTCCGCCATATGATGATGAATTGGTGATGGCAGGTCAGGGCACGATTGCCAAT
GAAATTCTGCGTCAGTGGCGTGATGTTGAATATGTATTTGTTGCCGTAGGTGGTGGTGGACTCATTGCAGGCGTTGCAGC
GTATTTGGGTGATGTTGCTCCTCACGTAAAAATCATTCCAGTCGAATATGATGAATCTGCTTGCCTGAAAGCCGCATTCG
AACACAATGAACGTGTTATTTTGCCACAAGTCGGTTTATTTGCAGATGGTACTGCGGTTGCACAAATTGGTGAAAAACCC
TTTGAAGTAATTCAGCTTCAAAAATCAGATAACTCTGGTCCAATTGTTGAACGCGATGTGATCTTGGTCAATACCGATGA
AATTTGTGCTGCCATCAAAGACACTTTTGATGAATGCCGTAGCATTGTAGAACCATCTGGTGCAATGGCGCTGGCAGGTA
TCAAAAAATATATCGAACAACATCAAATTACAGATAAAAATATCGTTTCGATTGTTTGCGGTGCAAACATGAATTTTGAC
CGATTGCGTTATATTGCTGAACGTACAGAACTTGGCGAACGTAAAGAGGCCATTTTTGCTGTCACCATTCCTGAACAAAA
AGGCGCCTTTTTAAATTTCTGCCGTGCATTACATGGACGCAATATTACAGAATTTAACTATCGTGCAAGCGATAGCGATG
ATGCTCAAGTTTTCGTCGGAATCAGTTTAAAATCTGGCGAAAAAGAACGTCATGACATTCACGAGTTACTTAAAAGCCAA
TATAATGTTGATGATTTATCCGATGACGAAGTTGCTAAGCTGCATATTCGCTATCTTATCGGTGGTCATGCCAATATCGA
AAATGAACGCTTGTTTCGTGTTGAATTTCCAGAGCGACCCGGTGCACTTCTAACCTTCCTTGAACGCCTTGGACCTAGTC
ATAATATTAGCCTGTTCCATTATCGTAATCACGGTGCTGCCGAAGGACGTGTATTGGTAGGTTTACAGGCTACCGATACG
CAAGAAAACCCAGATGTACTAATTGAAACATTGGAAAATATTACTTATCCATATGAAGAAATTACACACAATGTGGGTTA
TAAACGCTTTTTGCAGTAA

Upstream 100 bases:

>100_bases
CACTCAACTTTTTTTCACATTTTGCGCAATTTTAAAACGCAAAGGTGCAGGGGTTTGTTACAATAAGTGGCTATTTTAAA
CTGTTACATGGAAAATTAAC

Downstream 100 bases:

>100_bases
AAATCGATTGTTCTCACCAACCGACATTCTTGCGAACTGTCGGTTTTTTTATATCTTTTTAACATTTCTTTGCGCTAGGC
ATAGACAGAAAATACTACAC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLSRLVRQILQATVYDVAIETPLEAAPRISEKLNNNIRFKREDLQPVFSFKLRGAYNRISQLPKSQLERGVITASAGNHA
QGVALSGQKLGIRAIIVMPKTTPDIKVQAVKRLGGEVVLHGDSFDVANKYAIQRAQDEGMTFIPPYDDELVMAGQGTIAN
EILRQWRDVEYVFVAVGGGGLIAGVAAYLGDVAPHVKIIPVEYDESACLKAAFEHNERVILPQVGLFADGTAVAQIGEKP
FEVIQLQKSDNSGPIVERDVILVNTDEICAAIKDTFDECRSIVEPSGAMALAGIKKYIEQHQITDKNIVSIVCGANMNFD
RLRYIAERTELGERKEAIFAVTIPEQKGAFLNFCRALHGRNITEFNYRASDSDDAQVFVGISLKSGEKERHDIHELLKSQ
YNVDDLSDDEVAKLHIRYLIGGHANIENERLFRVEFPERPGALLTFLERLGPSHNISLFHYRNHGAAEGRVLVGLQATDT
QENPDVLIETLENITYPYEEITHNVGYKRFLQ

Sequences:

>Translated_512_residues
MLSRLVRQILQATVYDVAIETPLEAAPRISEKLNNNIRFKREDLQPVFSFKLRGAYNRISQLPKSQLERGVITASAGNHA
QGVALSGQKLGIRAIIVMPKTTPDIKVQAVKRLGGEVVLHGDSFDVANKYAIQRAQDEGMTFIPPYDDELVMAGQGTIAN
EILRQWRDVEYVFVAVGGGGLIAGVAAYLGDVAPHVKIIPVEYDESACLKAAFEHNERVILPQVGLFADGTAVAQIGEKP
FEVIQLQKSDNSGPIVERDVILVNTDEICAAIKDTFDECRSIVEPSGAMALAGIKKYIEQHQITDKNIVSIVCGANMNFD
RLRYIAERTELGERKEAIFAVTIPEQKGAFLNFCRALHGRNITEFNYRASDSDDAQVFVGISLKSGEKERHDIHELLKSQ
YNVDDLSDDEVAKLHIRYLIGGHANIENERLFRVEFPERPGALLTFLERLGPSHNISLFHYRNHGAAEGRVLVGLQATDT
QENPDVLIETLENITYPYEEITHNVGYKRFLQ
>Mature_512_residues
MLSRLVRQILQATVYDVAIETPLEAAPRISEKLNNNIRFKREDLQPVFSFKLRGAYNRISQLPKSQLERGVITASAGNHA
QGVALSGQKLGIRAIIVMPKTTPDIKVQAVKRLGGEVVLHGDSFDVANKYAIQRAQDEGMTFIPPYDDELVMAGQGTIAN
EILRQWRDVEYVFVAVGGGGLIAGVAAYLGDVAPHVKIIPVEYDESACLKAAFEHNERVILPQVGLFADGTAVAQIGEKP
FEVIQLQKSDNSGPIVERDVILVNTDEICAAIKDTFDECRSIVEPSGAMALAGIKKYIEQHQITDKNIVSIVCGANMNFD
RLRYIAERTELGERKEAIFAVTIPEQKGAFLNFCRALHGRNITEFNYRASDSDDAQVFVGISLKSGEKERHDIHELLKSQ
YNVDDLSDDEVAKLHIRYLIGGHANIENERLFRVEFPERPGALLTFLERLGPSHNISLFHYRNHGAAEGRVLVGLQATDT
QENPDVLIETLENITYPYEEITHNVGYKRFLQ

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=310, Percent_Identity=30, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI19923959, Length=325, Percent_Identity=28.3076923076923, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI33469958, Length=330, Percent_Identity=28.4848484848485, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1790207, Length=509, Percent_Identity=51.4734774066798, Blast_Score=480, Evalue=1e-136,
Organism=Escherichia coli, GI1789505, Length=320, Percent_Identity=35.9375, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789235, Length=154, Percent_Identity=33.1168831168831, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71991565, Length=387, Percent_Identity=32.8165374677003, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17508781, Length=295, Percent_Identity=28.135593220339, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17537387, Length=332, Percent_Identity=29.2168674698795, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17535051, Length=220, Percent_Identity=30, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320930, Length=523, Percent_Identity=49.1395793499044, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322631, Length=313, Percent_Identity=30.6709265175719, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319788, Length=276, Percent_Identity=25, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI21355833, Length=319, Percent_Identity=31.6614420062696, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645328, Length=260, Percent_Identity=28.8461538461538, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 57005; Mature: 57005

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRLVRQILQATVYDVAIETPLEAAPRISEKLNNNIRFKREDLQPVFSFKLRGAYNRIS
CHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHHHH
QLPKSQLERGVITASAGNHAQGVALSGQKLGIRAIIVMPKTTPDIKVQAVKRLGGEVVLH
HCCHHHHHCCEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEE
GDSFDVANKYAIQRAQDEGMTFIPPYDDELVMAGQGTIANEILRQWRDVEYVFVAVGGGG
CCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCH
LIAGVAAYLGDVAPHVKIIPVEYDESACLKAAFEHNERVILPQVGLFADGTAVAQIGEKP
HHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCEEECCHHHHHHCCCC
FEVIQLQKSDNSGPIVERDVILVNTDEICAAIKDTFDECRSIVEPSGAMALAGIKKYIEQ
EEEEEEEECCCCCCEEECCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HQITDKNIVSIVCGANMNFDRLRYIAERTELGERKEAIFAVTIPEQKGAFLNFCRALHGR
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCC
NITEFNYRASDSDDAQVFVGISLKSGEKERHDIHELLKSQYNVDDLSDDEVAKLHIRYLI
CCEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEE
GGHANIENERLFRVEFPERPGALLTFLERLGPSHNISLFHYRNHGAAEGRVLVGLQATDT
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECCCC
QENPDVLIETLENITYPYEEITHNVGYKRFLQ
CCCHHHHHHHHHHCCCCHHHHHHCCCHHHHCC
>Mature Secondary Structure
MLSRLVRQILQATVYDVAIETPLEAAPRISEKLNNNIRFKREDLQPVFSFKLRGAYNRIS
CHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHHHH
QLPKSQLERGVITASAGNHAQGVALSGQKLGIRAIIVMPKTTPDIKVQAVKRLGGEVVLH
HCCHHHHHCCEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEE
GDSFDVANKYAIQRAQDEGMTFIPPYDDELVMAGQGTIANEILRQWRDVEYVFVAVGGGG
CCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCH
LIAGVAAYLGDVAPHVKIIPVEYDESACLKAAFEHNERVILPQVGLFADGTAVAQIGEKP
HHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCEEECCHHHHHHCCCC
FEVIQLQKSDNSGPIVERDVILVNTDEICAAIKDTFDECRSIVEPSGAMALAGIKKYIEQ
EEEEEEEECCCCCCEEECCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HQITDKNIVSIVCGANMNFDRLRYIAERTELGERKEAIFAVTIPEQKGAFLNFCRALHGR
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCC
NITEFNYRASDSDDAQVFVGISLKSGEKERHDIHELLKSQYNVDDLSDDEVAKLHIRYLI
CCEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEE
GGHANIENERLFRVEFPERPGALLTFLERLGPSHNISLFHYRNHGAAEGRVLVGLQATDT
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECCCC
QENPDVLIETLENITYPYEEITHNVGYKRFLQ
CCCHHHHHHHHHHCCCCHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]