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The map label for this gene is rpoH [H]

Identifier: 50084493

GI number: 50084493

Start: 1311342

End: 1312211

Strand: Reverse

Name: rpoH [H]

Synonym: ACIAD1311

Alternate gene names: 50084493

Gene position: 1312211-1311342 (Counterclockwise)

Preceding gene: 50084494

Following gene: 50084492

Centisome position: 36.46

GC content: 42.99

Gene sequence:

>870_bases
ATGAGTGACAGCAGCAATCAGTTGATGCCCCTGTCCTTATCTGCGCCCGGTGTAAATCTTGGTGCCTATATCAGCACCGT
TAATCAAATTCCGATTTTAACGGCTGAGCAAGAGAAAGAGCTTGCTGAGCGTTATTATTATGATCAGGATCTTGATGCAG
CCAAAATGCTGGTCATGTCTCATCTTCGATTTGTAGTGCACATTGCGCGTAGTTATGCAGGTTATGGTTTACCTCAAGGC
GACCTGATTCAGGAAGGCAATCTCGGCTTGATGAAAGCCGTAAAACGTTTCGATCCAAATATGGGCGTGCGTCTGGTTTC
CTTCGCAGTTCACTGGATTAAGGCTGAAATTCACGAATACGTGATTCGCAACTGGCGTATTGTCAAAATTGCGACCACCA
AAGCTCAACGTAAATTGTTTTTCAATTTGCGTAGTCTAAAGAAATCAAGTAAAAAGCTGACTTTAGAAGAAGCACAATCG
ATTGCAAATGATTTAAACGTAACCCCTGAACAAGTTCTGGAAATGGAAGGCCGCTTAACCGCTTATGATGCAGCCTTTGA
AACGCAAGGTGATGACGACGATGACTCATCAACACATGTTGCACCAGCATTGTATCTTGAAGATAATCGTTATGATCCAG
CGCGTTTGGCTGAAAATGACGATTATGAAGAGCAAAGTTCAACAGCTCTGCATGAGGCTATGAATCAACTTGATGATCGT
TCGAGAAATATTTTACAGCGTCGCTGGCTAGATGATGACAAATCAACATTACATGAGCTCGCTGCGGAATATAACGTTTC
TGCCGAACGTATTCGACAGCTTGAAAAAAATGCCATGGAAAAAATCAAAGTTGCGATGTCCGCAGGCTAA

Upstream 100 bases:

>100_bases
CTTATAAAACTTTACATTTCTCTACCCAATTTTTCTTAATTATGAAGATGCTTCACATTAAGATGTTTATATTGGTTATA
CCATCCTACAAGGAGAACTC

Downstream 100 bases:

>100_bases
ATTATTTCGTTTAAGTCATATAGAAACTTAAGGGCATCATGCCCTTTTGTTTTGTTTAACTATTTTTAGCCAACCTGATT
TGTGCTAATGTTCTAGCGTC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQG
DLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQS
IANDLNVTPEQVLEMEGRLTAYDAAFETQGDDDDDSSTHVAPALYLEDNRYDPARLAENDDYEEQSSTALHEAMNQLDDR
SRNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAG

Sequences:

>Translated_289_residues
MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQG
DLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQS
IANDLNVTPEQVLEMEGRLTAYDAAFETQGDDDDDSSTHVAPALYLEDNRYDPARLAENDDYEEQSSTALHEAMNQLDDR
SRNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAG
>Mature_288_residues
SDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQGD
LIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQSI
ANDLNVTPEQVLEMEGRLTAYDAAFETQGDDDDDSSTHVAPALYLEDNRYDPARLAENDDYEEQSSTALHEAMNQLDDRS
RNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=58.5964912280702, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=29.2134831460674, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789448, Length=260, Percent_Identity=28.4615384615385, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32828; Mature: 32697

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMS
CCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEECHHHHHHHHHHCCCCCCHHHHHHHHH
HLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQSIANDLNVTPEQVLEMEGRLT
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCEE
AYDAAFETQGDDDDDSSTHVAPALYLEDNRYDPARLAENDDYEEQSSTALHEAMNQLDDR
EEHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SRNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAG
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMS
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEECHHHHHHHHHHCCCCCCHHHHHHHHH
HLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQSIANDLNVTPEQVLEMEGRLT
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCEE
AYDAAFETQGDDDDDSSTHVAPALYLEDNRYDPARLAENDDYEEQSSTALHEAMNQLDDR
EEHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SRNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAG
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7728657; 7698670; 10984043 [H]