Click here to switch to the map view.

The map label for this gene is thiG

Identifier: 50084490

GI number: 50084490

Start: 1309845

End: 1310630

Strand: Reverse

Name: thiG

Synonym: ACIAD1308

Alternate gene names: 50084490

Gene position: 1310630-1309845 (Counterclockwise)

Preceding gene: 50084491

Following gene: 50084489

Centisome position: 36.42

GC content: 42.88

Gene sequence:

>786_bases
ATGCAAGATTCACCTTTAATTATCGGTTCACGTCAGTTTCAATCTCGCCTTTTAGTCGGTACAGGCAAATATAAAGACCT
TAATGAAACTGATCTTGCAATTCAGGCGAGTGGTGCTGAAATCGTGACTGTCGCCATACGTCGCGTCAATATTGGACAAC
ATGCAGATCAACCTAATCTTCTTTCTGTAATTCCACCAGAAAAATATACGATTTTGCCAAATACTGCAGGTTGTTTTGAT
GCAGATAGTGCAATCCGAACTTGTATGCTGGCACGTGAACTACTCGATGGCCATAATTTAGTCAAGCTTGAAGTATTGGG
TGATCAAGACACGCTTTATCCGAATATCACGGAAACATTAAAAGCAGCACGTACACTGATTGATGATGGTTTTGAGATCA
TGGTATATACATCAGATGACCCAATTGTTGCCAAAGAGCTCGAAAGTATGGGATGTGTTGCCATCATGCCTTTGGGCAGT
CTGATTGGTTCTGGTTTAGGTATTTTAAATCCACATACCATTTCGATTATTAAGGAAAATGCCAAAGTTCCCGTGTTAGT
TGATGCAGGCGTAGGAACTGCTAGCGATGCTGCAATCGCAATGGAACTCGGCTGCGATGGCGTGCTGATGAATACAGCAA
TTGCAGCAGCTCAACATCCAGTTTTGATGGCATCTGCCATGAAAAAAGCGATTGAAGCAGGCCGAGAAGCCTTTTTAGCG
GGTCGTATGCCACGCAAACGAATGGCGAATGCCAGCTCTCCAGAAACAGGCTATTTCTTTAAATAA

Upstream 100 bases:

>100_bases
CAAATTATTCCCAAAAGTCGGTTAGCACTCACCCCTATTTCCGATCAAGATCGTATCGAAATTATTCATGCTGTCGGTGG
CGGCTAAAACGGAGTTTTTC

Downstream 100 bases:

>100_bases
CGCATACTATTATTATATTGAAAAGTTTCAAAGGATTCTTCGGAATCCTTTTTCATAAAAACAATGAGAGCAAGCGCAAA
TGCGAAAAATAATGATTGGT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MQDSPLIIGSRQFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQHADQPNLLSVIPPEKYTILPNTAGCFD
ADSAIRTCMLARELLDGHNLVKLEVLGDQDTLYPNITETLKAARTLIDDGFEIMVYTSDDPIVAKELESMGCVAIMPLGS
LIGSGLGILNPHTISIIKENAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQHPVLMASAMKKAIEAGREAFLA
GRMPRKRMANASSPETGYFFK

Sequences:

>Translated_261_residues
MQDSPLIIGSRQFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQHADQPNLLSVIPPEKYTILPNTAGCFD
ADSAIRTCMLARELLDGHNLVKLEVLGDQDTLYPNITETLKAARTLIDDGFEIMVYTSDDPIVAKELESMGCVAIMPLGS
LIGSGLGILNPHTISIIKENAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQHPVLMASAMKKAIEAGREAFLA
GRMPRKRMANASSPETGYFFK
>Mature_261_residues
MQDSPLIIGSRQFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQHADQPNLLSVIPPEKYTILPNTAGCFD
ADSAIRTCMLARELLDGHNLVKLEVLGDQDTLYPNITETLKAARTLIDDGFEIMVYTSDDPIVAKELESMGCVAIMPLGS
LIGSGLGILNPHTISIIKENAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQHPVLMASAMKKAIEAGREAFLA
GRMPRKRMANASSPETGYFFK

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=45.6692913385827, Blast_Score=201, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_ACIAD (Q6FCN1)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046000.1
- ProteinModelPortal:   Q6FCN1
- SMR:   Q6FCN1
- STRING:   Q6FCN1
- GeneID:   2880746
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1308
- NMPDR:   fig|62977.3.peg.482
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q6FCN1
- ProtClustDB:   PRK00208
- BioCyc:   ASP62977:ACIAD1308-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27811; Mature: 27811

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDSPLIIGSRQFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQHADQPNL
CCCCCEEEECHHHHCEEEEECCCCCCCCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCE
LSVIPPEKYTILPNTAGCFDADSAIRTCMLARELLDGHNLVKLEVLGDQDTLYPNITETL
EEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHH
KAARTLIDDGFEIMVYTSDDPIVAKELESMGCVAIMPLGSLIGSGLGILNPHTISIIKEN
HHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCEEEEEECC
AKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQHPVLMASAMKKAIEAGREAFLA
CCEEEEEECCCCCCCCCEEEEEECCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GRMPRKRMANASSPETGYFFK
CCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQDSPLIIGSRQFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQHADQPNL
CCCCCEEEECHHHHCEEEEECCCCCCCCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCE
LSVIPPEKYTILPNTAGCFDADSAIRTCMLARELLDGHNLVKLEVLGDQDTLYPNITETL
EEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHH
KAARTLIDDGFEIMVYTSDDPIVAKELESMGCVAIMPLGSLIGSGLGILNPHTISIIKEN
HHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCEEEEEECC
AKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQHPVLMASAMKKAIEAGREAFLA
CCEEEEEECCCCCCCCCEEEEEECCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GRMPRKRMANASSPETGYFFK
CCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA