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The map label for this gene is rnhB

Identifier: 50084434

GI number: 50084434

Start: 1247459

End: 1248028

Strand: Direct

Name: rnhB

Synonym: ACIAD1248

Alternate gene names: 50084434

Gene position: 1247459-1248028 (Clockwise)

Preceding gene: 50084433

Following gene: 50084440

Centisome position: 34.66

GC content: 42.28

Gene sequence:

>570_bases
ATGATTGCAGGTGTTGATGAAGCTGGTCGTGGTCCGCTGGTTGGGTCGGTTGTTGCTGCCGCGGTAATTTTGGATCCTCA
TAACCCAATCGATGGACTAAATGATTCAAAAAAACTGACTGAGAAAAAACGAGAGAAGCTTTTCGTAGAGATACAGGAGA
AGGCTTTGGCATGGTCAATTGCTGAAGCAAACCATATTGAAATTGATGAGTTGAATATTCTACAGGCAACGTTTCTGGCA
ATGCGTAGGGCAGTGGATGGATTGCAAATACAGCCTGATAAAGTGCTTGTTGATGGTAATCAGATTCCTAAGGGCATATC
CATCCACTGTGAAGCAATTGTAGGTGGAGATGCGAGCCATGCTGAAATCAGTGCTGCAAGTATTCTTGCCAAAGTATATC
GAGATCAGCAAATGAAACTGCTTGATGAGAAATATCCATTATTCGGATTTGCTAAACATAAAGGCTATCCGACTAAAGCA
CACTTTCAAGCAATTGCTATACATGGTGTAGTCGATGAGCATCGTAGAAGCTATGCACCTGTAAGACGGGCATTGAATAT
CGATTGTTAA

Upstream 100 bases:

>100_bases
CCAAATGCACGCGCTTCTGTGGTACAGCAAGTCGTATCAAATACAGTTACGTCATGTATTAATGAAACATTTGGTAAAAA
ATAAGTCAGATATCATGATA

Downstream 100 bases:

>100_bases
TCTTAGAATTTTAGCAATTCAGAAATGTTTAAAGCGGCTAGATTAGCCGCTTTAATTTGGGAGAAAAATTAAAAAATAAC
TTTAACGGTTAAAATTATTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MIAGVDEAGRGPLVGSVVAAAVILDPHNPIDGLNDSKKLTEKKREKLFVEIQEKALAWSIAEANHIEIDELNILQATFLA
MRRAVDGLQIQPDKVLVDGNQIPKGISIHCEAIVGGDASHAEISAASILAKVYRDQQMKLLDEKYPLFGFAKHKGYPTKA
HFQAIAIHGVVDEHRRSYAPVRRALNIDC

Sequences:

>Translated_189_residues
MIAGVDEAGRGPLVGSVVAAAVILDPHNPIDGLNDSKKLTEKKREKLFVEIQEKALAWSIAEANHIEIDELNILQATFLA
MRRAVDGLQIQPDKVLVDGNQIPKGISIHCEAIVGGDASHAEISAASILAKVYRDQQMKLLDEKYPLFGFAKHKGYPTKA
HFQAIAIHGVVDEHRRSYAPVRRALNIDC
>Mature_189_residues
MIAGVDEAGRGPLVGSVVAAAVILDPHNPIDGLNDSKKLTEKKREKLFVEIQEKALAWSIAEANHIEIDELNILQATFLA
MRRAVDGLQIQPDKVLVDGNQIPKGISIHCEAIVGGDASHAEISAASILAKVYRDQQMKLLDEKYPLFGFAKHKGYPTKA
HFQAIAIHGVVDEHRRSYAPVRRALNIDC

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=187, Percent_Identity=60.9625668449198, Blast_Score=228, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ACIAD (Q6FCT7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045944.1
- ProteinModelPortal:   Q6FCT7
- SMR:   Q6FCT7
- STRING:   Q6FCT7
- GeneID:   2879510
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1248
- NMPDR:   fig|62977.3.peg.317
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q6FCT7
- ProtClustDB:   PRK00015
- BioCyc:   ASP62977:ACIAD1248-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 20705; Mature: 20705

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGVDEAGRGPLVGSVVAAAVILDPHNPIDGLNDSKKLTEKKREKLFVEIQEKALAWSI
CCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
AEANHIEIDELNILQATFLAMRRAVDGLQIQPDKVLVDGNQIPKGISIHCEAIVGGDASH
CCCCCEEECHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCEEEEEEEECCCCCC
AEISAASILAKVYRDQQMKLLDEKYPLFGFAKHKGYPTKAHFQAIAIHGVVDEHRRSYAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEECHHHHHHHHHHH
VRRALNIDC
HHHHHCCCC
>Mature Secondary Structure
MIAGVDEAGRGPLVGSVVAAAVILDPHNPIDGLNDSKKLTEKKREKLFVEIQEKALAWSI
CCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
AEANHIEIDELNILQATFLAMRRAVDGLQIQPDKVLVDGNQIPKGISIHCEAIVGGDASH
CCCCCEEECHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCEEEEEEEECCCCCC
AEISAASILAKVYRDQQMKLLDEKYPLFGFAKHKGYPTKAHFQAIAIHGVVDEHRRSYAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEECHHHHHHHHHHH
VRRALNIDC
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA