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The map label for this gene is dacC [H]

Identifier: 50084413

GI number: 50084413

Start: 1226995

End: 1228143

Strand: Reverse

Name: dacC [H]

Synonym: ACIAD1225

Alternate gene names: 50084413

Gene position: 1228143-1226995 (Counterclockwise)

Preceding gene: 50084414

Following gene: 50084412

Centisome position: 34.13

GC content: 41.17

Gene sequence:

>1149_bases
ATGACTCTAAAAAGCGCCCTTGCTGCATTAGTTCTTTTACCAACATTTTCCTATGCAGCAACTGTCTTAACAACACCGCC
AGAATTAAATAACAAATCCTATGTGTTGATGGATTATGAGACAGGGCAAATTCTCGCCTCAAAAAATGAAAATGAACATC
TTGCTCCTGCCTCTATGACTAAAATGATGACCAGTTACATCATTGAGCAGAAATTGCTAAAGGGCGAGCTTACAGAAGGT
GAACAGGTTCGTATGAATGAATCGGCTTGGTGCCGTGGTAGCAGTTCTGAATCATGTATGTACGTACCGTTAAATGGCAC
CGCGACAGTTCTTGAAATGCTACGCGGGATTATCGTGCAATCAGGTAATGATGCATCTAAAGCCATGGCAGAACATGTGG
CTGGCAATGAGGGTACATTTGCGCACATGATGAACCAAGAAGCCAAACGTTTGGGTATGGTGAATACGCACTTTATGAAC
TCGACTGGCTTACCAGCAGAAGGTCATTACTCATCTGCCAAAGACATGGCGATTCTTGCACAGCATATTATCAAAGACAG
TTCAAAATACTATCCAATTTATTCAGAAAAAGAATTTACCTTTAATGGTATTAAGCAAGGCAACCGTAACGCATTACTTT
ATACCGACCCAAGCGTAGATGGATTAAAAACAGGTCATACCTCTGAAGCAGGTTATTGCCTGACCACATCCAGTAAACGT
GGTCCAATGCGTTTAATCTCGGTTATTTTTGGGACCACTAGTGTCAATGAACGCGCCAATCAAACCCGTACTTTACTGGC
TTGGGGCTTTTCAAACTTTGAAACTGTAAATGTACAACCTGCAAATCAAGTACTTGCTAAAGCCAAAGTATGGTTTGGTA
AGGAAAATGAAGTTCAGATTGGTTTAGCAGAGAACTTTAATGTCACCATGCCAAAAGGCCAAGCAGACAAAATCAAGACT
CAGCTTGTAGTACAGCCAAAATTGAATGCGCCATTACAAAAGGGTCAGGTTGTTGGCAAATTGGTCGCGACGCTTGATGG
TAAAGTAATTGCAGAAAAACCTTTGGTGGCTTTAAAACCAGTTGAAGAGGCAAGTTTCTTTGCGCGTATGATTGATCATA
TCAAACAATTCTTTAGCAACCTGTTCTAA

Upstream 100 bases:

>100_bases
TACTGTATGAAATAAGCCGACTTTCCCGTAGAAGTCGGCTTTGCTTTATCCTAAAATGCTAGGCTCTTCTGCTTTATTCA
AGATATTGGAACCACCTAGA

Downstream 100 bases:

>100_bases
TTGATCGCTATAAAAATGAAGGCATTCCTGTTTCAAACATGAATGCCTTTTTTCTTTTATACTGCATAAAACTCAACGAC
ATTGATAGCTCATGAAAAAA

Product: D-ala-D-ala-carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTLKSALAALVLLPTFSYAATVLTTPPELNNKSYVLMDYETGQILASKNENEHLAPASMTKMMTSYIIEQKLLKGELTEG
EQVRMNESAWCRGSSSESCMYVPLNGTATVLEMLRGIIVQSGNDASKAMAEHVAGNEGTFAHMMNQEAKRLGMVNTHFMN
STGLPAEGHYSSAKDMAILAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTSEAGYCLTTSSKR
GPMRLISVIFGTTSVNERANQTRTLLAWGFSNFETVNVQPANQVLAKAKVWFGKENEVQIGLAENFNVTMPKGQADKIKT
QLVVQPKLNAPLQKGQVVGKLVATLDGKVIAEKPLVALKPVEEASFFARMIDHIKQFFSNLF

Sequences:

>Translated_382_residues
MTLKSALAALVLLPTFSYAATVLTTPPELNNKSYVLMDYETGQILASKNENEHLAPASMTKMMTSYIIEQKLLKGELTEG
EQVRMNESAWCRGSSSESCMYVPLNGTATVLEMLRGIIVQSGNDASKAMAEHVAGNEGTFAHMMNQEAKRLGMVNTHFMN
STGLPAEGHYSSAKDMAILAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTSEAGYCLTTSSKR
GPMRLISVIFGTTSVNERANQTRTLLAWGFSNFETVNVQPANQVLAKAKVWFGKENEVQIGLAENFNVTMPKGQADKIKT
QLVVQPKLNAPLQKGQVVGKLVATLDGKVIAEKPLVALKPVEEASFFARMIDHIKQFFSNLF
>Mature_381_residues
TLKSALAALVLLPTFSYAATVLTTPPELNNKSYVLMDYETGQILASKNENEHLAPASMTKMMTSYIIEQKLLKGELTEGE
QVRMNESAWCRGSSSESCMYVPLNGTATVLEMLRGIIVQSGNDASKAMAEHVAGNEGTFAHMMNQEAKRLGMVNTHFMNS
TGLPAEGHYSSAKDMAILAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTSEAGYCLTTSSKRG
PMRLISVIFGTTSVNERANQTRTLLAWGFSNFETVNVQPANQVLAKAKVWFGKENEVQIGLAENFNVTMPKGQADKIKTQ
LVVQPKLNAPLQKGQVVGKLVATLDGKVIAEKPLVALKPVEEASFFARMIDHIKQFFSNLF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=367, Percent_Identity=42.7792915531335, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1787062, Length=385, Percent_Identity=40.7792207792208, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI87082022, Length=356, Percent_Identity=38.7640449438202, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI87082054, Length=257, Percent_Identity=29.1828793774319, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41949; Mature: 41818

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKSALAALVLLPTFSYAATVLTTPPELNNKSYVLMDYETGQILASKNENEHLAPASMT
CCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEECCCEEEECCCCCCCCCHHHHH
KMMTSYIIEQKLLKGELTEGEQVRMNESAWCRGSSSESCMYVPLNGTATVLEMLRGIIVQ
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHEEE
SGNDASKAMAEHVAGNEGTFAHMMNQEAKRLGMVNTHFMNSTGLPAEGHYSSAKDMAILA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
QHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTSEAGYCLTTSSKR
HHHHHCCCCCCCEECCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
GPMRLISVIFGTTSVNERANQTRTLLAWGFSNFETVNVQPANQVLAKAKVWFGKENEVQI
CHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHHEECCCCCEEE
GLAENFNVTMPKGQADKIKTQLVVQPKLNAPLQKGQVVGKLVATLDGKVIAEKPLVALKP
EEECCCEEECCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEECC
VEEASFFARMIDHIKQFFSNLF
CHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLKSALAALVLLPTFSYAATVLTTPPELNNKSYVLMDYETGQILASKNENEHLAPASMT
CHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEECCCEEEECCCCCCCCCHHHHH
KMMTSYIIEQKLLKGELTEGEQVRMNESAWCRGSSSESCMYVPLNGTATVLEMLRGIIVQ
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHEEE
SGNDASKAMAEHVAGNEGTFAHMMNQEAKRLGMVNTHFMNSTGLPAEGHYSSAKDMAILA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
QHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTSEAGYCLTTSSKR
HHHHHCCCCCCCEECCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
GPMRLISVIFGTTSVNERANQTRTLLAWGFSNFETVNVQPANQVLAKAKVWFGKENEVQI
CHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHHEECCCCCEEE
GLAENFNVTMPKGQADKIKTQLVVQPKLNAPLQKGQVVGKLVATLDGKVIAEKPLVALKP
EEECCCEEECCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEECC
VEEASFFARMIDHIKQFFSNLF
CHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]