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The map label for this gene is rnhA-dnaQ [H]

Identifier: 50084331

GI number: 50084331

Start: 1124144

End: 1125496

Strand: Reverse

Name: rnhA-dnaQ [H]

Synonym: ACIAD1137

Alternate gene names: 50084331

Gene position: 1125496-1124144 (Counterclockwise)

Preceding gene: 50084338

Following gene: 50084330

Centisome position: 31.28

GC content: 42.79

Gene sequence:

>1353_bases
ATGTCACAAACAATCACGCTTTATGTTGATGGCGCCTGTAAAGGAAATCCAGGCTTAGGTGGTTGGGGCGCGTATGTCAT
CACAGAGCAAGGCGAACATAAATTATGTGGTGGTGAAGCAGAAACGACCAATAATCGCATGGAGCTTACTGCTGCAATTG
AAGGAATACGCTTTTGCCCTCAAGATGCCTCCCTGATTATTTGGACAGACTCCAATTACGTTAAGCAAGGTATTACCGAA
TGGATTCATGGCTGGAAAAAGAAAAACTGGAAAGATGTAAAAAATCCTGATTTATGGCAAATCCTCGATCAACTTTGTCA
GGGTAGGCAAATTGAGTGGAACTGGATTAAAGGTCATGCGGGGCACGCTGGAAACGAAATGGCAGATGAGTTAGCCAATC
TTGGCGCAAGCCAAACTGCGGCTCAATTAAAGTCAGGCTCGGTAGCAGCAATTGATAAAAAAAAACCTCAAAATGACTGG
TTGCTCGACGATCCATTTGGCTTTGACATGATGACGAACGATATGGATACAGACGACACTTTGCAAGAAACAGAATTACA
AAAACATGTAGATCCGCGGTTAGAAAATGAAATACCTGAAACGTCAACAGAGAGCCTACATCCTCAAATTGTCATGACAC
CTGCCAAAATACAGGCTCAGGGTCCGCGTCAGCTCATTTTGGATACAGAAACAACGGGCTTTTATTTTCAGGACGGAGAT
CGAATTATTGAGGTAGGCGCGATTGAAATGATCAATCGTAAACTCACTGGAAGTTCAATCCACATTTATATCAATCCTGA
AAAACCTGTTGGTGATTCGGTGGACGTCCATGGAATTACCGATGAATTCCTACTTGATAAACCCAAATACGCTGAAATTG
CAGAGCCTTTATTTGAATACTTAAAAGGCGCAGAGATTATTGCCCATAACGCAAGCTTCGATATGAACTTCTTGGACATG
GAGTTTAAACGTGCGGGTTTACCGACACTATCCGAAGTGTGTGAAGTGACAGATACATTAGCACTGGCAAAAAATAAACA
TCCAGGGCAAAAAAACTCACTTGATGCATTGGTTAGACGATACGAAATACCAGCACGGGATCGTACTTTTCACGGTGCAT
TACTTGATGCCGAAATTCTGTCTGATGTGTATTTAGCCATGACGGGTGGACAGGTTTCATTTGATATGGATGCCCTGTCA
CAACGTGAACAAAATCAGAGAAAAGGCCAACGGGCTCGGATTGAAGCTGATTTACCTGTCATTCGGCCCTCAGCCAACGA
ACTCGAAAAACATAATGAATGGGTCAAAAGCTATCAGGAAAAAAATGGCGAGCCATGTCTATTTGCCAAATAA

Upstream 100 bases:

>100_bases
AAGAAATTAAAAAAACTGCCGCTTTTTAGCAGAGAACTTACCCATTTGCATATACAATACCAAGACTTATCTTCGTTTTT
CTGTGTCGGGTTCGGTAATT

Downstream 100 bases:

>100_bases
GCAAAAGATTTTCAAATGCGAATGATGCAAGCAGATAAACTTCAGGTATATTCAATCTATTGATTCATTCGCGCCTCACT
GGACGCTTAAAACATTATAA

Product: bifunctional ribonuclease HI/DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSQTITLYVDGACKGNPGLGGWGAYVITEQGEHKLCGGEAETTNNRMELTAAIEGIRFCPQDASLIIWTDSNYVKQGITE
WIHGWKKKNWKDVKNPDLWQILDQLCQGRQIEWNWIKGHAGHAGNEMADELANLGASQTAAQLKSGSVAAIDKKKPQNDW
LLDDPFGFDMMTNDMDTDDTLQETELQKHVDPRLENEIPETSTESLHPQIVMTPAKIQAQGPRQLILDTETTGFYFQDGD
RIIEVGAIEMINRKLTGSSIHIYINPEKPVGDSVDVHGITDEFLLDKPKYAEIAEPLFEYLKGAEIIAHNASFDMNFLDM
EFKRAGLPTLSEVCEVTDTLALAKNKHPGQKNSLDALVRRYEIPARDRTFHGALLDAEILSDVYLAMTGGQVSFDMDALS
QREQNQRKGQRARIEADLPVIRPSANELEKHNEWVKSYQEKNGEPCLFAK

Sequences:

>Translated_450_residues
MSQTITLYVDGACKGNPGLGGWGAYVITEQGEHKLCGGEAETTNNRMELTAAIEGIRFCPQDASLIIWTDSNYVKQGITE
WIHGWKKKNWKDVKNPDLWQILDQLCQGRQIEWNWIKGHAGHAGNEMADELANLGASQTAAQLKSGSVAAIDKKKPQNDW
LLDDPFGFDMMTNDMDTDDTLQETELQKHVDPRLENEIPETSTESLHPQIVMTPAKIQAQGPRQLILDTETTGFYFQDGD
RIIEVGAIEMINRKLTGSSIHIYINPEKPVGDSVDVHGITDEFLLDKPKYAEIAEPLFEYLKGAEIIAHNASFDMNFLDM
EFKRAGLPTLSEVCEVTDTLALAKNKHPGQKNSLDALVRRYEIPARDRTFHGALLDAEILSDVYLAMTGGQVSFDMDALS
QREQNQRKGQRARIEADLPVIRPSANELEKHNEWVKSYQEKNGEPCLFAK
>Mature_449_residues
SQTITLYVDGACKGNPGLGGWGAYVITEQGEHKLCGGEAETTNNRMELTAAIEGIRFCPQDASLIIWTDSNYVKQGITEW
IHGWKKKNWKDVKNPDLWQILDQLCQGRQIEWNWIKGHAGHAGNEMADELANLGASQTAAQLKSGSVAAIDKKKPQNDWL
LDDPFGFDMMTNDMDTDDTLQETELQKHVDPRLENEIPETSTESLHPQIVMTPAKIQAQGPRQLILDTETTGFYFQDGDR
IIEVGAIEMINRKLTGSSIHIYINPEKPVGDSVDVHGITDEFLLDKPKYAEIAEPLFEYLKGAEIIAHNASFDMNFLDME
FKRAGLPTLSEVCEVTDTLALAKNKHPGQKNSLDALVRRYEIPARDRTFHGALLDAEILSDVYLAMTGGQVSFDMDALSQ
REQNQRKGQRARIEADLPVIRPSANELEKHNEWVKSYQEKNGEPCLFAK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21359816, Length=156, Percent_Identity=35.2564102564103, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786409, Length=235, Percent_Identity=46.8085106382979, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786408, Length=141, Percent_Identity=48.936170212766, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=37.8571428571429, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17137476, Length=145, Percent_Identity=37.2413793103448, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45552509, Length=145, Percent_Identity=37.2413793103448, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 50470; Mature: 50339

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00018 EF_HAND_1 ; PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTITLYVDGACKGNPGLGGWGAYVITEQGEHKLCGGEAETTNNRMELTAAIEGIRFCP
CCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHCCC
QDASLIIWTDSNYVKQGITEWIHGWKKKNWKDVKNPDLWQILDQLCQGRQIEWNWIKGHA
CCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCC
GHAGNEMADELANLGASQTAAQLKSGSVAAIDKKKPQNDWLLDDPFGFDMMTNDMDTDDT
CCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCHH
LQETELQKHVDPRLENEIPETSTESLHPQIVMTPAKIQAQGPRQLILDTETTGFYFQDGD
HHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEECHHEECCCCCEEEEECCCCCEEEECCC
RIIEVGAIEMINRKLTGSSIHIYINPEKPVGDSVDVHGITDEFLLDKPKYAEIAEPLFEY
EEEEECHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCHHHHCCCCCHHHHHHHHHHH
LKGAEIIAHNASFDMNFLDMEFKRAGLPTLSEVCEVTDTLALAKNKHPGQKNSLDALVRR
HCCCEEEEECCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
YEIPARDRTFHGALLDAEILSDVYLAMTGGQVSFDMDALSQREQNQRKGQRARIEADLPV
HCCCCCCCCCCCHHHHHHHHHCCCEEEECCEEEECHHHHHHHHHHHHCCCCEEEECCCCE
IRPSANELEKHNEWVKSYQEKNGEPCLFAK
ECCCHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
SQTITLYVDGACKGNPGLGGWGAYVITEQGEHKLCGGEAETTNNRMELTAAIEGIRFCP
CCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHCCC
QDASLIIWTDSNYVKQGITEWIHGWKKKNWKDVKNPDLWQILDQLCQGRQIEWNWIKGHA
CCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCC
GHAGNEMADELANLGASQTAAQLKSGSVAAIDKKKPQNDWLLDDPFGFDMMTNDMDTDDT
CCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCHH
LQETELQKHVDPRLENEIPETSTESLHPQIVMTPAKIQAQGPRQLILDTETTGFYFQDGD
HHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEECHHEECCCCCEEEEECCCCCEEEECCC
RIIEVGAIEMINRKLTGSSIHIYINPEKPVGDSVDVHGITDEFLLDKPKYAEIAEPLFEY
EEEEECHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCHHHHCCCCCHHHHHHHHHHH
LKGAEIIAHNASFDMNFLDMEFKRAGLPTLSEVCEVTDTLALAKNKHPGQKNSLDALVRR
HCCCEEEEECCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
YEIPARDRTFHGALLDAEILSDVYLAMTGGQVSFDMDALSQREQNQRKGQRARIEADLPV
HCCCCCCCCCCCHHHHHHHHHCCCEEEECCEEEECHHHHHHHHHHHHCCCCEEEECCCCE
IRPSANELEKHNEWVKSYQEKNGEPCLFAK
ECCCHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3023634; 6316347; 3540531; 9278503; 1575709 [H]