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The map label for this gene is rlmH

Identifier: 50084307

GI number: 50084307

Start: 1100908

End: 1101387

Strand: Reverse

Name: rlmH

Synonym: ACIAD1113

Alternate gene names: 50084307

Gene position: 1101387-1100908 (Counterclockwise)

Preceding gene: 50084308

Following gene: 50084306

Centisome position: 30.61

GC content: 42.5

Gene sequence:

>480_bases
ATGAAAATCCGTATTCTTACCATCGGTCAAAAAATGCCAGCTTGGGTACTCACGGGTTTTGAAGATTATTTCAAACGTAT
CCAGCCTTTTGTACAAACTCAGGTGATTGAACTACCTATGGCAAAACGCGGAAAAAATGATTCAGATGCAGACATACTCA
AATATTGCCAGATTGAAGGTGAAAGTATCTTGGCAGCACTTAAGCCGAATGAAGTTCTGATTGCATTAGAAGTAGGTGGA
CGAGAGCTCAGTACAGAAAAACTGGCAGACACCATGAAATCATGGATGTTAGAAGGTCATGACATCGCTTTGGCAATTGG
TGGTCCAGATGGCCTATCAGATCAAGTGCGTAAAGCTGCTGCATGGCACTGGTCACTTTCAAAGCTCACCATGCCCCACC
CAATGGTTCGTATTTTACTCATTGAACAGCTTTATCGTGCAATGAGTATCAACCACAATCATCCATATCACCGTGCATAA

Upstream 100 bases:

>100_bases
AACGTTTAGACAGTTTAAGGGTCTTGAATTATATTCGTTATACTTGTTGTAATATTCATGGCTATACTATCTCGTTTTAA
TTCTGCCATTGGATATCTGT

Downstream 100 bases:

>100_bases
AAATTTGGGATTTGTTTGATCTTTTATATTTTAAATATCTGCGATCCAGCATGTTTCTTGCTTTCAAATATGACCTTAGT
ATGTTGATATAAAAATTAGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKIRILTIGQKMPAWVLTGFEDYFKRIQPFVQTQVIELPMAKRGKNDSDADILKYCQIEGESILAALKPNEVLIALEVGG
RELSTEKLADTMKSWMLEGHDIALAIGGPDGLSDQVRKAAAWHWSLSKLTMPHPMVRILLIEQLYRAMSINHNHPYHRA

Sequences:

>Translated_159_residues
MKIRILTIGQKMPAWVLTGFEDYFKRIQPFVQTQVIELPMAKRGKNDSDADILKYCQIEGESILAALKPNEVLIALEVGG
RELSTEKLADTMKSWMLEGHDIALAIGGPDGLSDQVRKAAAWHWSLSKLTMPHPMVRILLIEQLYRAMSINHNHPYHRA
>Mature_159_residues
MKIRILTIGQKMPAWVLTGFEDYFKRIQPFVQTQVIELPMAKRGKNDSDADILKYCQIEGESILAALKPNEVLIALEVGG
RELSTEKLADTMKSWMLEGHDIALAIGGPDGLSDQVRKAAAWHWSLSKLTMPHPMVRILLIEQLYRAMSINHNHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=158, Percent_Identity=47.4683544303797, Blast_Score=154, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ACIAD (O52828)

Other databases:

- EMBL:   AJ224767
- EMBL:   CR543861
- RefSeq:   YP_045817.1
- ProteinModelPortal:   O52828
- SMR:   O52828
- STRING:   O52828
- GeneID:   2878547
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1113
- NMPDR:   fig|62977.3.peg.1310
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   O52828
- ProtClustDB:   CLSK707202
- BioCyc:   ASP62977:ACIAD1113-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18018; Mature: 18018

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRILTIGQKMPAWVLTGFEDYFKRIQPFVQTQVIELPMAKRGKNDSDADILKYCQIEG
CEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCC
ESILAALKPNEVLIALEVGGRELSTEKLADTMKSWMLEGHDIALAIGGPDGLSDQVRKAA
CEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
AWHWSLSKLTMPHPMVRILLIEQLYRAMSINHNHPYHRA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIRILTIGQKMPAWVLTGFEDYFKRIQPFVQTQVIELPMAKRGKNDSDADILKYCQIEG
CEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCC
ESILAALKPNEVLIALEVGGRELSTEKLADTMKSWMLEGHDIALAIGGPDGLSDQVRKAA
CEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
AWHWSLSKLTMPHPMVRILLIEQLYRAMSINHNHPYHRA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA