Click here to switch to the map view.

The map label for this gene is lipA [H]

Identifier: 50084218

GI number: 50084218

Start: 1006746

End: 1007777

Strand: Reverse

Name: lipA [H]

Synonym: ACIAD1015

Alternate gene names: 50084218

Gene position: 1007777-1006746 (Counterclockwise)

Preceding gene: 50084226

Following gene: 50084217

Centisome position: 28.0

GC content: 40.89

Gene sequence:

>1032_bases
ATGGATTTAAAAATAAATGACCAACCCGATAAAAATATTTATCTTGGTGAAAAACTACGTGGGCAGGAAAAGCTCGAGCG
TATTCCTGTTAAAATTATTCCTAGTACTGAAATAGCAGAAAAACCAGATTGGATTCGTACCAAAATCAGTGATCCCAAAG
AAATCAAACGTATTCAGACTTTATTACGTCAACAAAAATTGCATACGGTGTGCGAAGAAGCTGCTTGTCCTAACTTGCCA
CAATGTTTTGGAAGTGGCACAGCCACTTTTATGATTATGGGCGATATCTGCACTCGGCGCTGCCCTTTTTGCGATGTCGC
ACATGGTCGGCCCAATACACTCGACCCATTAGAACCCCAACATTTAGCAGAAGCAGTATCCAATCTTCAGCTTCAATATG
TGGTTATCACCTCGGTAGATCGAGATGATCTTAAGGATGGAGGAGCGCAGCATTTTGTTGATTGCATAAAAGCAATTCGT
GCAACCAATCCTCATACATTAATCGAAATTTTAGTGCCTGATTTTCGTGGTCGACTCGAAATAGCTTTAAAAATTCTAGC
CAGCTCTCCGCCTGATGTTTTTAATCACAATATCGAAACTGTTCCACGTTTGTATCGCGCCCTGCGACCAGGTTCTGATT
ACCAACATTCATTAAAGCTTTTACAGCAATTTAAAAAATATTGTCCAGATGTCGTCACCAAATGTGGTCTGATGGTTGGA
CTTGGTGAAGTAGAAGCTGAAGTCATTACCTTACTCAATGATCTGCGCGATCATGATATTGAGCTGGTCACCATTGGACA
ATACTTACAACCCTCCAAAGCGCATGCACCTGTACATCGATTTGTTTCACCAACAGAATTTGAACATTATGAACAGCATG
GCAAACGTCTAGGTTTTAAAAATATCTGGAGTGCACCGCTGGTTCGCTCCAGTTATCATGCAGATCGTCAATACCGAGGC
GAAGCTGTACCGAAGCCTTTTACACGAGAAGATTTAAAACAACTAAAACATACCACATCTGATATTGCTTAA

Upstream 100 bases:

>100_bases
ATTATTTTAAACACGATTCAACTTAATTATTCAAACCAGATATTTAATTAAGTTTAAATTATATTCAAATATTAATGTTA
AAATTAAAAGGATTTTTAGA

Downstream 100 bases:

>100_bases
CGATTTATGAACATTTATCTATATGCTTACTTCCCGCTTCATGCTACAAATAAATGTCTAAATCTTGGTGATCTTGCCCA
GATCCCAAGATATGTCTATA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDLKINDQPDKNIYLGEKLRGQEKLERIPVKIIPSTEIAEKPDWIRTKISDPKEIKRIQTLLRQQKLHTVCEEAACPNLP
QCFGSGTATFMIMGDICTRRCPFCDVAHGRPNTLDPLEPQHLAEAVSNLQLQYVVITSVDRDDLKDGGAQHFVDCIKAIR
ATNPHTLIEILVPDFRGRLEIALKILASSPPDVFNHNIETVPRLYRALRPGSDYQHSLKLLQQFKKYCPDVVTKCGLMVG
LGEVEAEVITLLNDLRDHDIELVTIGQYLQPSKAHAPVHRFVSPTEFEHYEQHGKRLGFKNIWSAPLVRSSYHADRQYRG
EAVPKPFTREDLKQLKHTTSDIA

Sequences:

>Translated_343_residues
MDLKINDQPDKNIYLGEKLRGQEKLERIPVKIIPSTEIAEKPDWIRTKISDPKEIKRIQTLLRQQKLHTVCEEAACPNLP
QCFGSGTATFMIMGDICTRRCPFCDVAHGRPNTLDPLEPQHLAEAVSNLQLQYVVITSVDRDDLKDGGAQHFVDCIKAIR
ATNPHTLIEILVPDFRGRLEIALKILASSPPDVFNHNIETVPRLYRALRPGSDYQHSLKLLQQFKKYCPDVVTKCGLMVG
LGEVEAEVITLLNDLRDHDIELVTIGQYLQPSKAHAPVHRFVSPTEFEHYEQHGKRLGFKNIWSAPLVRSSYHADRQYRG
EAVPKPFTREDLKQLKHTTSDIA
>Mature_343_residues
MDLKINDQPDKNIYLGEKLRGQEKLERIPVKIIPSTEIAEKPDWIRTKISDPKEIKRIQTLLRQQKLHTVCEEAACPNLP
QCFGSGTATFMIMGDICTRRCPFCDVAHGRPNTLDPLEPQHLAEAVSNLQLQYVVITSVDRDDLKDGGAQHFVDCIKAIR
ATNPHTLIEILVPDFRGRLEIALKILASSPPDVFNHNIETVPRLYRALRPGSDYQHSLKLLQQFKKYCPDVVTKCGLMVG
LGEVEAEVITLLNDLRDHDIELVTIGQYLQPSKAHAPVHRFVSPTEFEHYEQHGKRLGFKNIWSAPLVRSSYHADRQYRG
EAVPKPFTREDLKQLKHTTSDIA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=296, Percent_Identity=43.5810810810811, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=44.8979591836735, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI1786846, Length=311, Percent_Identity=55.9485530546624, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32564533, Length=312, Percent_Identity=42.6282051282051, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=292, Percent_Identity=40.7534246575342, Blast_Score=235, Evalue=8e-63,
Organism=Drosophila melanogaster, GI221513272, Length=280, Percent_Identity=45.7142857142857, Blast_Score=260, Evalue=9e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 38991; Mature: 38991

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKINDQPDKNIYLGEKLRGQEKLERIPVKIIPSTEIAEKPDWIRTKISDPKEIKRIQT
CCCCCCCCCCCCEECCHHHCCHHHHHHCCEEEECCCHHCCCCCHHHHCCCCHHHHHHHHH
LLRQQKLHTVCEEAACPNLPQCFGSGTATFMIMGDICTRRCPFCDVAHGRPNTLDPLEPQ
HHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCHH
HLAEAVSNLQLQYVVITSVDRDDLKDGGAQHFVDCIKAIRATNPHTLIEILVPDFRGRLE
HHHHHHHCCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHH
IALKILASSPPDVFNHNIETVPRLYRALRPGSDYQHSLKLLQQFKKYCPDVVTKCGLMVG
HHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGEVEAEVITLLNDLRDHDIELVTIGQYLQPSKAHAPVHRFVSPTEFEHYEQHGKRLGFK
CCHHHHHHHHHHHHHHHCCEEEEECCCHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCHH
NIWSAPLVRSSYHADRQYRGEAVPKPFTREDLKQLKHTTSDIA
HHHCCCHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLKINDQPDKNIYLGEKLRGQEKLERIPVKIIPSTEIAEKPDWIRTKISDPKEIKRIQT
CCCCCCCCCCCCEECCHHHCCHHHHHHCCEEEECCCHHCCCCCHHHHCCCCHHHHHHHHH
LLRQQKLHTVCEEAACPNLPQCFGSGTATFMIMGDICTRRCPFCDVAHGRPNTLDPLEPQ
HHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCHH
HLAEAVSNLQLQYVVITSVDRDDLKDGGAQHFVDCIKAIRATNPHTLIEILVPDFRGRLE
HHHHHHHCCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHH
IALKILASSPPDVFNHNIETVPRLYRALRPGSDYQHSLKLLQQFKKYCPDVVTKCGLMVG
HHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGEVEAEVITLLNDLRDHDIELVTIGQYLQPSKAHAPVHRFVSPTEFEHYEQHGKRLGFK
CCHHHHHHHHHHHHHHHCCEEEEECCCHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCHH
NIWSAPLVRSSYHADRQYRGEAVPKPFTREDLKQLKHTTSDIA
HHHCCCHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA