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The map label for this gene is ilvB [H]

Identifier: 50084202

GI number: 50084202

Start: 984177

End: 985817

Strand: Direct

Name: ilvB [H]

Synonym: ACIAD0999

Alternate gene names: 50084202

Gene position: 984177-985817 (Clockwise)

Preceding gene: 50084201

Following gene: 50084203

Centisome position: 27.35

GC content: 45.89

Gene sequence:

>1641_bases
ATGACTGAACGTGTCAATGTTGGAGAGGCGATTGCCCGTTTACTTGAAGCCCATGATGTAAATAGTATTTATGGTGTCAT
TTCGATTCATAATTTACCTATTGCCGATGCAGTGGGGCGCCGTGAAAAAATCCGTTTTGTCGCAGCGCGTGGTGAAGCTG
GTGCGGTGACGATGGCCGATGCACATGCCCGTTTTAAAGGCTTGGGTGTAGCACTGACCAGTACAGGTGCGGGTGCGGGC
AACGCAGTAGGTTCACTGATTGAGGCCATGAATGCAGCAAGCCCAGTGTTGCATTTAACAGGTCAGGTTGAGCGTGAATA
TTTAGACCGTGATGCCAGTTTTATTCATGAAACCAAAGATCAGCTTAGCTTCCTGCGTGCATCGAGCAAGGCTGCTTTTC
GTATTACCAGTCCAGACAATGCGATTGGAGTAATTCGTGAAGCGATTCGTGTAGCAACGACGGTTCCCATGGGACCCGTC
AGTGTGGAATTGCCTATTGATGTTCAGGCAGCAGAAATTGATTTACCTGCAGATTTAAGTCCTGTACAAGCCATGCAATT
GCCGCAGGTCGCGCAGAGTCAGGTTGATTTTCTGGCAGATCAAATTAAGCAAGCCAAACGTCCTGTATTCTGGATTGGTG
GTGGTGCCCTCAATTGCGTCGATGAAATCAAAGCCATTGCAGATCTGGGCATTCCTATCGTGTCATCTACACATGGTCGC
GGGATTTTACCTGATGCGCATCCTCGTAGTTTAGGTGCTTTTCATAACTCAGCAAAAGTTGAAGAACTTCTAAAAGCATC
AGACTTATTGGTTGTTGTGGGTTCACGCTTGCGCAGTAATGAAACCAAAACCTACTCGGTTCAATTTCCCGACAACTTGA
TTCAGATTGATGCAAATCCAGTGGCGCAACAGCGTAACTATAAGGTTCGCCGTTTTATTTGTGCAGATGCCAAGGATATT
TTAGTGCGTACTCTAACGCAGCTTGAAGGTATTTCTAAGGTAGATACCGAATATGATCAGATGATTGTTAATGCCAAAGA
TGCCGCGGTTGCAGCGCTTCGCAAGCAATTGGATCAATATGCCTTAATTTGTGATCACTTACGTTCGGCTTTACCAGAAG
ATGGCATCTTTGTACGTGACATTACCATGTCGGGGAGTACCTGGGGCAGTCGTTTATTTCCAGTTCAGGCACCAAATCGC
AATATCCACTCACTGGCAGGCGCAATCGGTCTAGGCTTGGCACATGCCATTGGCTCTAGTATTGCAAATCCAGATAAAAA
AGTAGTCGGGCTTGTGGGGGATGGTGGCTTGATGCTCGGGATTGGTGAAATCGCGACCATGGCACAAGAAAACACAGATA
TGGTTTTGATGATCATGAATGATGGTGGTTATGGTGTGATGCGTGGCATTCAAAATAATTATTTTGGGGGACGTCAGTAT
TTCAATGAATTGCATACGCCTGACTATCAAAAACTCGGAGAGTCTATGGGGGTTAAAAGCTGGAAAGTTGGCAGTGCCGA
TGAATTCAAGATTGCCATCAAAGATGCCATTGCTTTTGATGGTCCAGCAGTTATTGAACTCGATATGCATGCCATTGGCC
CATTAAATTTTGCTGGGCCACCACAGAAAAAACTGTACTAA

Upstream 100 bases:

>100_bases
TGGGGCTAAATGAACAGCCAAACCCGTGGGCAGATTAAGCCAATTTTAGCCAAGATATTTATTTAACAAAATTTTATACA
AAATTCAGAGGAAGATTTTC

Downstream 100 bases:

>100_bases
TCCCAAAGCCTAATAAAAAGACATTGGAATAGAACAAAGTAATGAGTCAAAAAGATGCTTGATCTGCCAAGGCAGGTCGA
GCAGCCTGAAACAGGGGATT

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTERVNVGEAIARLLEAHDVNSIYGVISIHNLPIADAVGRREKIRFVAARGEAGAVTMADAHARFKGLGVALTSTGAGAG
NAVGSLIEAMNAASPVLHLTGQVEREYLDRDASFIHETKDQLSFLRASSKAAFRITSPDNAIGVIREAIRVATTVPMGPV
SVELPIDVQAAEIDLPADLSPVQAMQLPQVAQSQVDFLADQIKQAKRPVFWIGGGALNCVDEIKAIADLGIPIVSSTHGR
GILPDAHPRSLGAFHNSAKVEELLKASDLLVVVGSRLRSNETKTYSVQFPDNLIQIDANPVAQQRNYKVRRFICADAKDI
LVRTLTQLEGISKVDTEYDQMIVNAKDAAVAALRKQLDQYALICDHLRSALPEDGIFVRDITMSGSTWGSRLFPVQAPNR
NIHSLAGAIGLGLAHAIGSSIANPDKKVVGLVGDGGLMLGIGEIATMAQENTDMVLMIMNDGGYGVMRGIQNNYFGGRQY
FNELHTPDYQKLGESMGVKSWKVGSADEFKIAIKDAIAFDGPAVIELDMHAIGPLNFAGPPQKKLY

Sequences:

>Translated_546_residues
MTERVNVGEAIARLLEAHDVNSIYGVISIHNLPIADAVGRREKIRFVAARGEAGAVTMADAHARFKGLGVALTSTGAGAG
NAVGSLIEAMNAASPVLHLTGQVEREYLDRDASFIHETKDQLSFLRASSKAAFRITSPDNAIGVIREAIRVATTVPMGPV
SVELPIDVQAAEIDLPADLSPVQAMQLPQVAQSQVDFLADQIKQAKRPVFWIGGGALNCVDEIKAIADLGIPIVSSTHGR
GILPDAHPRSLGAFHNSAKVEELLKASDLLVVVGSRLRSNETKTYSVQFPDNLIQIDANPVAQQRNYKVRRFICADAKDI
LVRTLTQLEGISKVDTEYDQMIVNAKDAAVAALRKQLDQYALICDHLRSALPEDGIFVRDITMSGSTWGSRLFPVQAPNR
NIHSLAGAIGLGLAHAIGSSIANPDKKVVGLVGDGGLMLGIGEIATMAQENTDMVLMIMNDGGYGVMRGIQNNYFGGRQY
FNELHTPDYQKLGESMGVKSWKVGSADEFKIAIKDAIAFDGPAVIELDMHAIGPLNFAGPPQKKLY
>Mature_545_residues
TERVNVGEAIARLLEAHDVNSIYGVISIHNLPIADAVGRREKIRFVAARGEAGAVTMADAHARFKGLGVALTSTGAGAGN
AVGSLIEAMNAASPVLHLTGQVEREYLDRDASFIHETKDQLSFLRASSKAAFRITSPDNAIGVIREAIRVATTVPMGPVS
VELPIDVQAAEIDLPADLSPVQAMQLPQVAQSQVDFLADQIKQAKRPVFWIGGGALNCVDEIKAIADLGIPIVSSTHGRG
ILPDAHPRSLGAFHNSAKVEELLKASDLLVVVGSRLRSNETKTYSVQFPDNLIQIDANPVAQQRNYKVRRFICADAKDIL
VRTLTQLEGISKVDTEYDQMIVNAKDAAVAALRKQLDQYALICDHLRSALPEDGIFVRDITMSGSTWGSRLFPVQAPNRN
IHSLAGAIGLGLAHAIGSSIANPDKKVVGLVGDGGLMLGIGEIATMAQENTDMVLMIMNDGGYGVMRGIQNNYFGGRQYF
NELHTPDYQKLGESMGVKSWKVGSADEFKIAIKDAIAFDGPAVIELDMHAIGPLNFAGPPQKKLY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=564, Percent_Identity=24.290780141844, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=23.2682060390764, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1790104, Length=541, Percent_Identity=25.8780036968577, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI87081685, Length=536, Percent_Identity=25.3731343283582, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=23.4119782214156, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=24.2477876106195, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788716, Length=543, Percent_Identity=24.1252302025783, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17542570, Length=554, Percent_Identity=25.6317689530686, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=543, Percent_Identity=23.572744014733, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=543, Percent_Identity=23.572744014733, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=561, Percent_Identity=25.3119429590018, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6323073, Length=531, Percent_Identity=25.0470809792844, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=544, Percent_Identity=23.3455882352941, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=558, Percent_Identity=24.910394265233, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320123, Length=548, Percent_Identity=21.7153284671533, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320816, Length=554, Percent_Identity=22.0216606498195, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=561, Percent_Identity=22.8163992869875, Blast_Score=86, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58612; Mature: 58481

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERVNVGEAIARLLEAHDVNSIYGVISIHNLPIADAVGRREKIRFVAARGEAGAVTMAD
CCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCCCEEEEEEECCCCCEEEEEC
AHARFKGLGVALTSTGAGAGNAVGSLIEAMNAASPVLHLTGQVEREYLDRDASFIHETKD
HHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHCCHHHHHHHHHH
QLSFLRASSKAAFRITSPDNAIGVIREAIRVATTVPMGPVSVELPIDVQAAEIDLPADLS
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCC
PVQAMQLPQVAQSQVDFLADQIKQAKRPVFWIGGGALNCVDEIKAIADLGIPIVSSTHGR
HHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCCC
GILPDAHPRSLGAFHNSAKVEELLKASDLLVVVGSRLRSNETKTYSVQFPDNLIQIDANP
CCCCCCCCCHHHHHCCHHHHHHHHHHCCEEEEECCHHCCCCCEEEEEECCCCEEEECCCC
VAQQRNYKVRRFICADAKDILVRTLTQLEGISKVDTEYDQMIVNAKDAAVAALRKQLDQY
HHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHHCCCHHHHEECCHHHHHHHHHHHHHHH
ALICDHLRSALPEDGIFVRDITMSGSTWGSRLFPVQAPNRNIHSLAGAIGLGLAHAIGSS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
IANPDKKVVGLVGDGGLMLGIGEIATMAQENTDMVLMIMNDGGYGVMRGIQNNYFGGRQY
HCCCCCEEEEEEECCCEEEEHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCCCHHHH
FNELHTPDYQKLGESMGVKSWKVGSADEFKIAIKDAIAFDGPAVIELDMHAIGPLNFAGP
HHHHCCCCHHHHHHHCCCCCEECCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCC
PQKKLY
CHHCCC
>Mature Secondary Structure 
TERVNVGEAIARLLEAHDVNSIYGVISIHNLPIADAVGRREKIRFVAARGEAGAVTMAD
CCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCCCEEEEEEECCCCCEEEEEC
AHARFKGLGVALTSTGAGAGNAVGSLIEAMNAASPVLHLTGQVEREYLDRDASFIHETKD
HHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHCCHHHHHHHHHH
QLSFLRASSKAAFRITSPDNAIGVIREAIRVATTVPMGPVSVELPIDVQAAEIDLPADLS
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCC
PVQAMQLPQVAQSQVDFLADQIKQAKRPVFWIGGGALNCVDEIKAIADLGIPIVSSTHGR
HHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCCC
GILPDAHPRSLGAFHNSAKVEELLKASDLLVVVGSRLRSNETKTYSVQFPDNLIQIDANP
CCCCCCCCCHHHHHCCHHHHHHHHHHCCEEEEECCHHCCCCCEEEEEECCCCEEEECCCC
VAQQRNYKVRRFICADAKDILVRTLTQLEGISKVDTEYDQMIVNAKDAAVAALRKQLDQY
HHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHHCCCHHHHEECCHHHHHHHHHHHHHHH
ALICDHLRSALPEDGIFVRDITMSGSTWGSRLFPVQAPNRNIHSLAGAIGLGLAHAIGSS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
IANPDKKVVGLVGDGGLMLGIGEIATMAQENTDMVLMIMNDGGYGVMRGIQNNYFGGRQY
HCCCCCEEEEEEECCCEEEEHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCCCHHHH
FNELHTPDYQKLGESMGVKSWKVGSADEFKIAIKDAIAFDGPAVIELDMHAIGPLNFAGP
HHHHCCCCHHHHHHHCCCCCEECCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCC
PQKKLY
CHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]