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The map label for this gene is thcD [H]

Identifier: 50084195

GI number: 50084195

Start: 977070

End: 978281

Strand: Direct

Name: thcD [H]

Synonym: ACIAD0991

Alternate gene names: 50084195

Gene position: 977070-978281 (Clockwise)

Preceding gene: 50084194

Following gene: 50084196

Centisome position: 27.15

GC content: 43.23

Gene sequence:

>1212_bases
ATGGAAAGAATTGTGGTGGTCGGTGCAGGTCAGGCCGCAGGATGGGCAGTCAGTACTTTGCGTCAGGAAGGTTTTCAAGG
TGAGATTCATGTGGTGTCCAATGAACATCAGATTTTTTATGAACGCCCCCCATTGTCTAAGCAAGTGCTGTCCAAAGAAG
CGAGTTATGACAGCCTGAATTTATTTTCACCTGAACAAATTGATGCATTTTCTGTGCAATGGCACAAACCAGATCTGGCT
ACCCAGATTGATCGTGATCATAAACAGGTCATTTTAGAAAGTGGCAAAATTTTACCTTATGACAAACTCTTGATCGCGAC
AGGCAGCCGGGCACGGGTACCTGTGAATACCTGGAAAGATATTTCTAATGTTGTGACTTTACGTGATGTACAAGACTGCG
AACGTTTGGCGAATTTACTGGAAACAGCAAAGTCGATTGCCATCATTGGTGGCGGCTGGATTGGTCTTGAAATTGCCGCG
ACAGCACGTAAACAGGGCAAACAAGTTCATGTGTTTGAATACGCAGAACGCTTGTGTGGGCGCAGTGTCAGTCCAGAAGT
TTCTGAATTTTTAAAAGAAATTCATGAGCAGCAAGGCACAAAGATTCACCTTAATAGTAAAAATCTACATTTGATTGAAA
GTTCGGATCAAACGGTTCAGGTCCTCAACCATCCAGAAACAGCCCAGACATTTGATTGTGTGGTGGTGGGTGCAGGTGCA
GAAATTGCCAAAGAACTTGGGGTGAGTGCTGGTCTGGCTGTAAAAGATGGCATCGTGGTCAACTGTTATGGTCAAACCTC
AGATGTAGATATCTATGCTGCAGGTGATGTGGCGATTCATCCTGTTTTAGGGTACTGCATTCAGTCTTGGGCCAATGCAC
AAAATCAAGCCATTGCAGCTGCCAAATCAATGCTGGGTGTTGCGACCGAATATTCAGATATTCCATGGTTATGGTCAGAT
CAGTATCAGTTCAACATTCAGATTTTGGGTACCTATCAAGCTCAGTCTAATCCTAAATTAATTATTCGCCAGAGTAGTCC
AGCTCAACTCAGTTATTTATATCTGGATGAGCACAATCGCTTGATGAATCTAATTGCAGTCAATGATGCCAAGCTGGTCA
AACTGGCCAAACGCTGGATTCAAAACAGCACTGTATTAGATCCAAAATTATTAGCTGATCCTGAATTTAATGTCATGAAG
CTCAAACCGTAA

Upstream 100 bases:

>100_bases
CTATTTACCGAATGGGCGATTGAAGGTGGGCTTGATCATGATACCCGTCGTCAGATCAAACCAGTTCAGGGTTAATCAGG
TTTACAGGGAGTATACGTCA

Downstream 100 bases:

>100_bases
GCGCACTTCACGACATCCAGTCAGAGCACTCATTTTAGGCGAATCGCCAGATCGAAGGAATGTTGCATGTTAAATGATAT
AGATGAAAAAAAAGGATTCA

Product: ferredoxin reductase subunit of phenylpropionate dioxygenase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MERIVVVGAGQAAGWAVSTLRQEGFQGEIHVVSNEHQIFYERPPLSKQVLSKEASYDSLNLFSPEQIDAFSVQWHKPDLA
TQIDRDHKQVILESGKILPYDKLLIATGSRARVPVNTWKDISNVVTLRDVQDCERLANLLETAKSIAIIGGGWIGLEIAA
TARKQGKQVHVFEYAERLCGRSVSPEVSEFLKEIHEQQGTKIHLNSKNLHLIESSDQTVQVLNHPETAQTFDCVVVGAGA
EIAKELGVSAGLAVKDGIVVNCYGQTSDVDIYAAGDVAIHPVLGYCIQSWANAQNQAIAAAKSMLGVATEYSDIPWLWSD
QYQFNIQILGTYQAQSNPKLIIRQSSPAQLSYLYLDEHNRLMNLIAVNDAKLVKLAKRWIQNSTVLDPKLLADPEFNVMK
LKP

Sequences:

>Translated_403_residues
MERIVVVGAGQAAGWAVSTLRQEGFQGEIHVVSNEHQIFYERPPLSKQVLSKEASYDSLNLFSPEQIDAFSVQWHKPDLA
TQIDRDHKQVILESGKILPYDKLLIATGSRARVPVNTWKDISNVVTLRDVQDCERLANLLETAKSIAIIGGGWIGLEIAA
TARKQGKQVHVFEYAERLCGRSVSPEVSEFLKEIHEQQGTKIHLNSKNLHLIESSDQTVQVLNHPETAQTFDCVVVGAGA
EIAKELGVSAGLAVKDGIVVNCYGQTSDVDIYAAGDVAIHPVLGYCIQSWANAQNQAIAAAKSMLGVATEYSDIPWLWSD
QYQFNIQILGTYQAQSNPKLIIRQSSPAQLSYLYLDEHNRLMNLIAVNDAKLVKLAKRWIQNSTVLDPKLLADPEFNVMK
LKP
>Mature_403_residues
MERIVVVGAGQAAGWAVSTLRQEGFQGEIHVVSNEHQIFYERPPLSKQVLSKEASYDSLNLFSPEQIDAFSVQWHKPDLA
TQIDRDHKQVILESGKILPYDKLLIATGSRARVPVNTWKDISNVVTLRDVQDCERLANLLETAKSIAIIGGGWIGLEIAA
TARKQGKQVHVFEYAERLCGRSVSPEVSEFLKEIHEQQGTKIHLNSKNLHLIESSDQTVQVLNHPETAQTFDCVVVGAGA
EIAKELGVSAGLAVKDGIVVNCYGQTSDVDIYAAGDVAIHPVLGYCIQSWANAQNQAIAAAKSMLGVATEYSDIPWLWSD
QYQFNIQILGTYQAQSNPKLIIRQSSPAQLSYLYLDEHNRLMNLIAVNDAKLVKLAKRWIQNSTVLDPKLLADPEFNVMK
LKP

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=336, Percent_Identity=28.2738095238095, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI65787454, Length=336, Percent_Identity=28.2738095238095, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI226437568, Length=336, Percent_Identity=28.2738095238095, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788892, Length=411, Percent_Identity=33.8199513381995, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789065, Length=303, Percent_Identity=28.0528052805281, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789765, Length=399, Percent_Identity=24.5614035087719, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789915, Length=187, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=347, Percent_Identity=23.0547550432277, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639257, Length=401, Percent_Identity=21.4463840399002, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281359715, Length=401, Percent_Identity=21.4463840399002, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281359713, Length=401, Percent_Identity=21.4463840399002, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24639250, Length=401, Percent_Identity=21.4463840399002, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18543267, Length=401, Percent_Identity=21.4463840399002, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24639252, Length=401, Percent_Identity=21.4463840399002, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585130, Length=325, Percent_Identity=21.8461538461538, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 44606; Mature: 44606

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIVVVGAGQAAGWAVSTLRQEGFQGEIHVVSNEHQIFYERPPLSKQVLSKEASYDSLN
CCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHHHHHCCCCCCEE
LFSPEQIDAFSVQWHKPDLATQIDRDHKQVILESGKILPYDKLLIATGSRARVPVNTWKD
CCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHH
ISNVVTLRDVQDCERLANLLETAKSIAIIGGGWIGLEIAATARKQGKQVHVFEYAERLCG
HHHEEEEHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEEEHHHCCCEEEHHHHHHHHHC
RSVSPEVSEFLKEIHEQQGTKIHLNSKNLHLIESSDQTVQVLNHPETAQTFDCVVVGAGA
CCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCEEEEEEEECCH
EIAKELGVSAGLAVKDGIVVNCYGQTSDVDIYAAGDVAIHPVLGYCIQSWANAQNQAIAA
HHHHHHCCCCCCEEECCEEEEEECCCCCEEEEEECCEEEHHHHHHHHHHHCCCCHHHHHH
AKSMLGVATEYSDIPWLWSDQYQFNIQILGTYQAQSNPKLIIRQSSPAQLSYLYLDEHNR
HHHHHHHHCCCCCCCCEECCCEEEEEEEEEEEECCCCCEEEEECCCCCEEEEEEEECCCC
LMNLIAVNDAKLVKLAKRWIQNSTVLDPKLLADPEFNVMKLKP
EEEEEEECCHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEECC
>Mature Secondary Structure
MERIVVVGAGQAAGWAVSTLRQEGFQGEIHVVSNEHQIFYERPPLSKQVLSKEASYDSLN
CCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHHHHHCCCCCCEE
LFSPEQIDAFSVQWHKPDLATQIDRDHKQVILESGKILPYDKLLIATGSRARVPVNTWKD
CCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHH
ISNVVTLRDVQDCERLANLLETAKSIAIIGGGWIGLEIAATARKQGKQVHVFEYAERLCG
HHHEEEEHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEEEHHHCCCEEEHHHHHHHHHC
RSVSPEVSEFLKEIHEQQGTKIHLNSKNLHLIESSDQTVQVLNHPETAQTFDCVVVGAGA
CCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCEEEEEEEECCH
EIAKELGVSAGLAVKDGIVVNCYGQTSDVDIYAAGDVAIHPVLGYCIQSWANAQNQAIAA
HHHHHHCCCCCCEEECCEEEEEECCCCCEEEEEECCEEEHHHHHHHHHHHCCCCHHHHHH
AKSMLGVATEYSDIPWLWSDQYQFNIQILGTYQAQSNPKLIIRQSSPAQLSYLYLDEHNR
HHHHHHHHCCCCCCCCEECCCEEEEEEEEEEEECCCCCEEEEECCCCCEEEEEEEECCCC
LMNLIAVNDAKLVKLAKRWIQNSTVLDPKLLADPEFNVMKLKP
EEEEEEECCHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]