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The map label for this gene is emrB [H]

Identifier: 50084135

GI number: 50084135

Start: 909460

End: 911007

Strand: Direct

Name: emrB [H]

Synonym: ACIAD0927

Alternate gene names: 50084135

Gene position: 909460-911007 (Clockwise)

Preceding gene: 50084134

Following gene: 50084136

Centisome position: 25.27

GC content: 44.9

Gene sequence:

>1548_bases
ATGACCGCTCAAAGTCAGGCTTCTTCGACCAAAACCCCTTTTGCTGAACTCAGTGGCGGACGATTACTGCTCGCTGCATT
TGTGATTGCACTTTCCAACTTTATGGTGGTGCTGGATACCACGATTGCCAATGTATCTGTGCCACATATTACTGGAAATT
TGGCTGTTTCAGCATCACAAGGCACATGGGTGGTGACGTCCTATGCCGTTGCAGAAGCCATCTGCGTGCCGCTAACAGGC
TGGCTTGCCAGTAGGTTTGGAACAGTACGTGTATTTGTAATGGGGCTGATTGGATTTACAGTTTTTTCTTTTTTATGTGG
ACTCTCGACCAGTTTAGAAATGCTGGTTTTGTGTCGGATTGGGCAGGGTTTGTCAGGTGGGCCATTGATGCCACTCAGTC
AAACGTTGCTGATGCGGATTTTCCCGCAAGAAAAGCATTCACAAGCGATGGGCTTATGGGCAATGACCACCGTTTTGGGG
CCGATATTAGGTCCAATTTTAGGTGGGTTAATTAGTGATAACCTGTCATGGCACTGGATTTTCTTTATTAATATTCCTGT
GGGTATTATCTGTGTACTGGGTACGATTCGGCTTTTGAGCGTAGCAGAAACGGAGACAATTCGTGTCCGAATAGATGCGA
TTGGGTTGTTTTTACTGGTGCTATGGATTGGTGCATTGCAGCTGATGCTGGATTTAGGGCACGAACGTGACTGGTTTAAT
AGCAGTATTATTGTTTTATTTGCAGTGATTGCAGTACTCGGATTTATCGTATTTTTAATCTGGGAAATGACGGATCGGCT
GCCAGTGGTCAATCTGTGGGTATTTAGACACAGAGGCTTTACCGTTTCTGTGCTGGCATTATCTTTTGGCTTTGGTGCGT
TCTTTGGCAGTATTGTATTGATTCCGCAGTGGTTACAGATGAATCTTAACTATACTGCAACATGGGCAGGCTACCTGACC
GCCACCATGGGTTTTGGTAGTTTGATGATGTCACCCGTGGTGGCGAAACTGGCAGCGAAATATGATCCGCGAGCTCTGGC
GAGTTTTGGGTTGTTGCTACTCGGGGGAGTCACGCTCATGCGCGCTTTCTGGACATCAGATGCTGACTTTATGACGCTAG
CCTGGCCGCAGATTTTGCAAGGGTTTGCTGTTCCGTTTTTCTTTATTCCATTGTCTAATCTGGCACTTTCTTCGGTACAA
CCTCAAGAGCTTGCCTCTGCAGCAGGTTTAATGAACTTTTTAAGAACGATGGCAGGTGCAATAGGTGCTTCTATTGCAGT
TACTATATGGGACGATCATGCCAAAGTAGCACGTAGTGAAATGGTCTCAAACCTACATTCAGATGATGTTTACAATACCC
TGATTCAAAGCGGTTTTTCTTCAGATACAGCATTAGGTACCATTTCAAATTTAGTGGATAAAGAGTCGATTACGATGTCG
TCAGATCATGTATTCCTCATATTTTCACTCGTATTTGTATTTGCTGGATTGATCATCTGGTTAAGCCCTAAACCAAAGCA
GCAATTAAAAGGGCCTGCACCACATTAA

Upstream 100 bases:

>100_bases
AGTGCGTATCGCACTTGATCCGAAAGAATTGGCTGAGCATCCGTTACGTGTTGGGCTGTCTATGGATGCTAGCATCGATT
TATCCAAGAAGTAACCTATT

Downstream 100 bases:

>100_bases
GCATTTTTTTACCAAGCGTTATGACATCAATGTGATAATGCTTGATCTATTTTTTTAAATTATCTTAATAAAATTGAATA
AGTTAATCTGTTTAGTGCTG

Product: major facilitator superfamily multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTAQSQASSTKTPFAELSGGRLLLAAFVIALSNFMVVLDTTIANVSVPHITGNLAVSASQGTWVVTSYAVAEAICVPLTG
WLASRFGTVRVFVMGLIGFTVFSFLCGLSTSLEMLVLCRIGQGLSGGPLMPLSQTLLMRIFPQEKHSQAMGLWAMTTVLG
PILGPILGGLISDNLSWHWIFFINIPVGIICVLGTIRLLSVAETETIRVRIDAIGLFLLVLWIGALQLMLDLGHERDWFN
SSIIVLFAVIAVLGFIVFLIWEMTDRLPVVNLWVFRHRGFTVSVLALSFGFGAFFGSIVLIPQWLQMNLNYTATWAGYLT
ATMGFGSLMMSPVVAKLAAKYDPRALASFGLLLLGGVTLMRAFWTSDADFMTLAWPQILQGFAVPFFFIPLSNLALSSVQ
PQELASAAGLMNFLRTMAGAIGASIAVTIWDDHAKVARSEMVSNLHSDDVYNTLIQSGFSSDTALGTISNLVDKESITMS
SDHVFLIFSLVFVFAGLIIWLSPKPKQQLKGPAPH

Sequences:

>Translated_515_residues
MTAQSQASSTKTPFAELSGGRLLLAAFVIALSNFMVVLDTTIANVSVPHITGNLAVSASQGTWVVTSYAVAEAICVPLTG
WLASRFGTVRVFVMGLIGFTVFSFLCGLSTSLEMLVLCRIGQGLSGGPLMPLSQTLLMRIFPQEKHSQAMGLWAMTTVLG
PILGPILGGLISDNLSWHWIFFINIPVGIICVLGTIRLLSVAETETIRVRIDAIGLFLLVLWIGALQLMLDLGHERDWFN
SSIIVLFAVIAVLGFIVFLIWEMTDRLPVVNLWVFRHRGFTVSVLALSFGFGAFFGSIVLIPQWLQMNLNYTATWAGYLT
ATMGFGSLMMSPVVAKLAAKYDPRALASFGLLLLGGVTLMRAFWTSDADFMTLAWPQILQGFAVPFFFIPLSNLALSSVQ
PQELASAAGLMNFLRTMAGAIGASIAVTIWDDHAKVARSEMVSNLHSDDVYNTLIQSGFSSDTALGTISNLVDKESITMS
SDHVFLIFSLVFVFAGLIIWLSPKPKQQLKGPAPH
>Mature_514_residues
TAQSQASSTKTPFAELSGGRLLLAAFVIALSNFMVVLDTTIANVSVPHITGNLAVSASQGTWVVTSYAVAEAICVPLTGW
LASRFGTVRVFVMGLIGFTVFSFLCGLSTSLEMLVLCRIGQGLSGGPLMPLSQTLLMRIFPQEKHSQAMGLWAMTTVLGP
ILGPILGGLISDNLSWHWIFFINIPVGIICVLGTIRLLSVAETETIRVRIDAIGLFLLVLWIGALQLMLDLGHERDWFNS
SIIVLFAVIAVLGFIVFLIWEMTDRLPVVNLWVFRHRGFTVSVLALSFGFGAFFGSIVLIPQWLQMNLNYTATWAGYLTA
TMGFGSLMMSPVVAKLAAKYDPRALASFGLLLLGGVTLMRAFWTSDADFMTLAWPQILQGFAVPFFFIPLSNLALSSVQP
QELASAAGLMNFLRTMAGAIGASIAVTIWDDHAKVARSEMVSNLHSDDVYNTLIQSGFSSDTALGTISNLVDKESITMSS
DHVFLIFSLVFVFAGLIIWLSPKPKQQLKGPAPH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=495, Percent_Identity=44.040404040404, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1788710, Length=508, Percent_Identity=38.9763779527559, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=27.4647887323944, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI87081983, Length=399, Percent_Identity=27.5689223057644, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=31.8518518518519, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI87082320, Length=177, Percent_Identity=24.8587570621469, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=126, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=509, Percent_Identity=21.6110019646365, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55841; Mature: 55709

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQSQASSTKTPFAELSGGRLLLAAFVIALSNFMVVLDTTIANVSVPHITGNLAVSASQ
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCHHEEEEHHHHCCCCCEEECCEEEEECC
GTWVVTSYAVAEAICVPLTGWLASRFGTVRVFVMGLIGFTVFSFLCGLSTSLEMLVLCRI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GQGLSGGPLMPLSQTLLMRIFPQEKHSQAMGLWAMTTVLGPILGPILGGLISDNLSWHWI
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FFINIPVGIICVLGTIRLLSVAETETIRVRIDAIGLFLLVLWIGALQLMLDLGHERDWFN
EEEECCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SSIIVLFAVIAVLGFIVFLIWEMTDRLPVVNLWVFRHRGFTVSVLALSFGFGAFFGSIVL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHHHHHH
IPQWLQMNLNYTATWAGYLTATMGFGSLMMSPVVAKLAAKYDPRALASFGLLLLGGVTLM
CCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RAFWTSDADFMTLAWPQILQGFAVPFFFIPLSNLALSSVQPQELASAAGLMNFLRTMAGA
HHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IGASIAVTIWDDHAKVARSEMVSNLHSDDVYNTLIQSGFSSDTALGTISNLVDKESITMS
HCCEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEC
SDHVFLIFSLVFVFAGLIIWLSPKPKQQLKGPAPH
CCCHHHHHHHHHHHHHHHEECCCCCHHHCCCCCCC
>Mature Secondary Structure 
TAQSQASSTKTPFAELSGGRLLLAAFVIALSNFMVVLDTTIANVSVPHITGNLAVSASQ
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCHHEEEEHHHHCCCCCEEECCEEEEECC
GTWVVTSYAVAEAICVPLTGWLASRFGTVRVFVMGLIGFTVFSFLCGLSTSLEMLVLCRI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GQGLSGGPLMPLSQTLLMRIFPQEKHSQAMGLWAMTTVLGPILGPILGGLISDNLSWHWI
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FFINIPVGIICVLGTIRLLSVAETETIRVRIDAIGLFLLVLWIGALQLMLDLGHERDWFN
EEEECCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SSIIVLFAVIAVLGFIVFLIWEMTDRLPVVNLWVFRHRGFTVSVLALSFGFGAFFGSIVL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHHHHHH
IPQWLQMNLNYTATWAGYLTATMGFGSLMMSPVVAKLAAKYDPRALASFGLLLLGGVTLM
CCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RAFWTSDADFMTLAWPQILQGFAVPFFFIPLSNLALSSVQPQELASAAGLMNFLRTMAGA
HHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IGASIAVTIWDDHAKVARSEMVSNLHSDDVYNTLIQSGFSSDTALGTISNLVDKESITMS
HCCEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEC
SDHVFLIFSLVFVFAGLIIWLSPKPKQQLKGPAPH
CCCHHHHHHHHHHHHHHHEECCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]