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The map label for this gene is yafJ [C]

Identifier: 50084114

GI number: 50084114

Start: 888813

End: 889670

Strand: Direct

Name: yafJ [C]

Synonym: ACIAD0905

Alternate gene names: 50084114

Gene position: 888813-889670 (Clockwise)

Preceding gene: 50084113

Following gene: 50084115

Centisome position: 24.7

GC content: 44.06

Gene sequence:

>858_bases
TTGATTGTTATGTGCCAGCTACTTGGAATGAATTGTGCAACCCCGACAGACATCACCTTTTCGTTTAAGGGGTTTTCACA
GCGTGCTGGCATTACCTCTGATCATTCAGATGGCTTTGGTATCGCCTTTTTTGAAGATAAAACCTGTCGTTTATTTATCG
ACAATCAATCTGCTGTTGAATCTCCAATTGCCGAACTGGTAAGAAACTTTCCAATTAAATCACGTAATGTGATTGCGCAT
ATCCGTAAAGCGACGCAAGGTAAAATTACGCTGGAAAACTCACATCCATTCATGCGTGAACTTTGGGGGCGGCAATGGAT
TTTCGCACATAATGGAGATTTACATGGTTTTGATCCGCGTTTGACAGGTCGTTTTGTACCTGTGGGTAATACCGACAGCG
AACGGGCATTTTGTTATGTGCTGGATCAACTGGTGGAACGCTTTGGCTATACAGAGCCGAGTGTACTTCAGGTTTTTGCG
ACGCTAGAACAGATTGCACCGCGTGTTGCCGAATACGGAACCTTTAATTTCTGTTTATCGAATGGACAGGCATTATTTAG
TTATGCCACCACCAAGTTACACTGGCTGGTTCGTGAATATCCTTTTAAAGCAGCGCGTTTGATTGATATTGATGTCGAAG
TCGATTTTAGCCAGGTCACGACAGCTAAAGATCGTGTTGCGGTGATTACTACTGAACCCTTGACGCATAACGAAATCTGG
ACGCCTTATCAGCCAGGTGAAATAATCCTGTTTCAGGATGGACGTCCTGTAAAACGTGCTTTAACCCATGTTGCGCGTCT
GACACGTGAAGCACTCAATCCCGAATTAAAACGGATCACACGTGCAGACCAGTATTAA

Upstream 100 bases:

>100_bases
AGTCTTAGCAGAAAAAGTGCATAAGATGTGCCAAAATTGAGATTTGCAACACCTTCTACTCTCATGCAAAATAGTGTTTG
AATCTCGATAATTATTTAAT

Downstream 100 bases:

>100_bases
GTGATTAGATCTAATTAAAACATCTATTTTTAGAGAAGCTTTGATTTTCATACACGACAAAGGCAAAATATCGGCAGTCT
TTATTCAAGGAATGCATGAT

Product: glutamine amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MIVMCQLLGMNCATPTDITFSFKGFSQRAGITSDHSDGFGIAFFEDKTCRLFIDNQSAVESPIAELVRNFPIKSRNVIAH
IRKATQGKITLENSHPFMRELWGRQWIFAHNGDLHGFDPRLTGRFVPVGNTDSERAFCYVLDQLVERFGYTEPSVLQVFA
TLEQIAPRVAEYGTFNFCLSNGQALFSYATTKLHWLVREYPFKAARLIDIDVEVDFSQVTTAKDRVAVITTEPLTHNEIW
TPYQPGEIILFQDGRPVKRALTHVARLTREALNPELKRITRADQY

Sequences:

>Translated_285_residues
MIVMCQLLGMNCATPTDITFSFKGFSQRAGITSDHSDGFGIAFFEDKTCRLFIDNQSAVESPIAELVRNFPIKSRNVIAH
IRKATQGKITLENSHPFMRELWGRQWIFAHNGDLHGFDPRLTGRFVPVGNTDSERAFCYVLDQLVERFGYTEPSVLQVFA
TLEQIAPRVAEYGTFNFCLSNGQALFSYATTKLHWLVREYPFKAARLIDIDVEVDFSQVTTAKDRVAVITTEPLTHNEIW
TPYQPGEIILFQDGRPVKRALTHVARLTREALNPELKRITRADQY
>Mature_285_residues
MIVMCQLLGMNCATPTDITFSFKGFSQRAGITSDHSDGFGIAFFEDKTCRLFIDNQSAVESPIAELVRNFPIKSRNVIAH
IRKATQGKITLENSHPFMRELWGRQWIFAHNGDLHGFDPRLTGRFVPVGNTDSERAFCYVLDQLVERFGYTEPSVLQVFA
TLEQIAPRVAEYGTFNFCLSNGQALFSYATTKLHWLVREYPFKAARLIDIDVEVDFSQVTTAKDRVAVITTEPLTHNEIW
TPYQPGEIILFQDGRPVKRALTHVARLTREALNPELKRITRADQY

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=51.1811023622047, Blast_Score=276, Evalue=7e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 32467; Mature: 32467

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVMCQLLGMNCATPTDITFSFKGFSQRAGITSDHSDGFGIAFFEDKTCRLFIDNQSAVE
CEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCHHHH
SPIAELVRNFPIKSRNVIAHIRKATQGKITLENSHPFMRELWGRQWIFAHNGDLHGFDPR
HHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCEEEEEECCCCCCCCCC
LTGRFVPVGNTDSERAFCYVLDQLVERFGYTEPSVLQVFATLEQIAPRVAEYGTFNFCLS
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
NGQALFSYATTKLHWLVREYPFKAARLIDIDVEVDFSQVTTAKDRVAVITTEPLTHNEIW
CCCEEEHHHHHHHHHHHHHCCCCEEEEEEEEEEECHHHHCCCCCCEEEEEECCCCCCCCC
TPYQPGEIILFQDGRPVKRALTHVARLTREALNPELKRITRADQY
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MIVMCQLLGMNCATPTDITFSFKGFSQRAGITSDHSDGFGIAFFEDKTCRLFIDNQSAVE
CEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCHHHH
SPIAELVRNFPIKSRNVIAHIRKATQGKITLENSHPFMRELWGRQWIFAHNGDLHGFDPR
HHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCEEEEEECCCCCCCCCC
LTGRFVPVGNTDSERAFCYVLDQLVERFGYTEPSVLQVFATLEQIAPRVAEYGTFNFCLS
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
NGQALFSYATTKLHWLVREYPFKAARLIDIDVEVDFSQVTTAKDRVAVITTEPLTHNEIW
CCCEEEHHHHHHHHHHHHHCCCCEEEEEEEEEEECHHHHCCCCCCEEEEEECCCCCCCCC
TPYQPGEIILFQDGRPVKRALTHVARLTREALNPELKRITRADQY
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]