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The map label for this gene is dusC [H]

Identifier: 50084050

GI number: 50084050

Start: 824367

End: 825299

Strand: Reverse

Name: dusC [H]

Synonym: ACIAD0837

Alternate gene names: 50084050

Gene position: 825299-824367 (Counterclockwise)

Preceding gene: 50084051

Following gene: 50084049

Centisome position: 22.93

GC content: 44.8

Gene sequence:

>933_bases
TTGAAACTTATTCTTGCACCAATGGAAGGCTTGACCGATCCAATCATGCGTGATGTTCTTACGCATGTCGGAAGTTTCGA
CTGGTGCGTTACAGAGTTTATTCGTGTGACTGATAGCGTATTGCCTGATCATGTTTATTACACCTACTGCCCTGAGCTTA
AAAATAACGGTAAAACTGCTGCTGGTACGCCAGTTCATGTTCAGTTTTTAGGGAACAATCCCGATATGCTGGCTGCCAAT
GCCGAAAAAGTGGTGGCTTTGGGCGCACCAGCGATTGATCTGAATTTTGGCTGCCCTGCAAAAACCGTAAACCGACATCG
TGGTGGCTCAATCCTACTTGATGAGCCTGATGTCGTGCATCTACTCGTAAAAGCTGTGCGTGATGCCGTACCTGCACATA
TCCCAGTGTCTGCAAAAATGCGTCTGGGTTATCTCGATGAAAATCACACCATGGACAATGCTTATGCAATACAGGAAGCT
GGTGCAAACTGGTTGACGGTTCATGCGCGGACCAAAGCAGATGGTTATACGCCGCCTGCATATTGGGAAAAAATACAGCC
GATTAAAGATGCCTTAAGCATCAATGTGATTGCCAATGGTGAAATCTGGACCAATCAAGATGCAAAAGCCTGTCAGCAGC
AATCTGGCTGTGAAGATTTGATGATTGGCCGTGGTGCAGTCACCACGCCTGACCTTACTCTGTGCATCCGTCAAAATGTG
GATGAAGCCTTAATGAATTGGGCCGCTTTGATTGAACTACAACAGCGCTTTTTAAATGGTGAATATAAAACAGAAATTGG
CATGATTGGCCGTTATAAACAATGGCTAGGTATGATGACCAAAGCCTATCCTGAAGCCAAACAGCTTTGGGATGAGATTA
AACGCATCAAAAACCTAGACGAAATTTTATTGAAACTTGGTCGCTCATCATAA

Upstream 100 bases:

>100_bases
TCCAAAAGTAAATCGAATGAAGAAGAAATCTCAAACTGGACATCTCAGATAAAAATGCGTATAACGCTCAGGTTTATTTA
GCCCAGTCAGGTAATACACG

Downstream 100 bases:

>100_bases
TTTTTGATTCAGCCTCATCAAAAGCAAAACTTTCTACAAAAAACCGAAAATATGGTATTTCCTTAGAATTTCTTGATCAT
TTCAACCTTTAAAAAAAGAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKLILAPMEGLTDPIMRDVLTHVGSFDWCVTEFIRVTDSVLPDHVYYTYCPELKNNGKTAAGTPVHVQFLGNNPDMLAAN
AEKVVALGAPAIDLNFGCPAKTVNRHRGGSILLDEPDVVHLLVKAVRDAVPAHIPVSAKMRLGYLDENHTMDNAYAIQEA
GANWLTVHARTKADGYTPPAYWEKIQPIKDALSINVIANGEIWTNQDAKACQQQSGCEDLMIGRGAVTTPDLTLCIRQNV
DEALMNWAALIELQQRFLNGEYKTEIGMIGRYKQWLGMMTKAYPEAKQLWDEIKRIKNLDEILLKLGRSS

Sequences:

>Translated_310_residues
MKLILAPMEGLTDPIMRDVLTHVGSFDWCVTEFIRVTDSVLPDHVYYTYCPELKNNGKTAAGTPVHVQFLGNNPDMLAAN
AEKVVALGAPAIDLNFGCPAKTVNRHRGGSILLDEPDVVHLLVKAVRDAVPAHIPVSAKMRLGYLDENHTMDNAYAIQEA
GANWLTVHARTKADGYTPPAYWEKIQPIKDALSINVIANGEIWTNQDAKACQQQSGCEDLMIGRGAVTTPDLTLCIRQNV
DEALMNWAALIELQQRFLNGEYKTEIGMIGRYKQWLGMMTKAYPEAKQLWDEIKRIKNLDEILLKLGRSS
>Mature_310_residues
MKLILAPMEGLTDPIMRDVLTHVGSFDWCVTEFIRVTDSVLPDHVYYTYCPELKNNGKTAAGTPVHVQFLGNNPDMLAAN
AEKVVALGAPAIDLNFGCPAKTVNRHRGGSILLDEPDVVHLLVKAVRDAVPAHIPVSAKMRLGYLDENHTMDNAYAIQEA
GANWLTVHARTKADGYTPPAYWEKIQPIKDALSINVIANGEIWTNQDAKACQQQSGCEDLMIGRGAVTTPDLTLCIRQNV
DEALMNWAALIELQQRFLNGEYKTEIGMIGRYKQWLGMMTKAYPEAKQLWDEIKRIKNLDEILLKLGRSS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=230, Percent_Identity=30.8695652173913, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI40807366, Length=192, Percent_Identity=30.7291666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI8923374, Length=258, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788462, Length=305, Percent_Identity=44.2622950819672, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1789660, Length=230, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693211, Length=252, Percent_Identity=26.5873015873016, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=174, Percent_Identity=35.632183908046, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=171, Percent_Identity=32.7485380116959, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=176, Percent_Identity=32.3863636363636, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=177, Percent_Identity=31.0734463276836, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19921524, Length=172, Percent_Identity=30.8139534883721, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=310, Percent_Identity=28.0645161290323, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24580595, Length=193, Percent_Identity=26.9430051813472, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920448, Length=193, Percent_Identity=26.9430051813472, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34485; Mature: 34485

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLILAPMEGLTDPIMRDVLTHVGSFDWCVTEFIRVTDSVLPDHVYYTYCPELKNNGKTA
CEEEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
AGTPVHVQFLGNNPDMLAANAEKVVALGAPAIDLNFGCPAKTVNRHRGGSILLDEPDVVH
CCCCEEEEEECCCCCEEECCCCEEEEECCCEEECCCCCCHHHHHHCCCCEEEECCCHHHH
LLVKAVRDAVPAHIPVSAKMRLGYLDENHTMDNAYAIQEAGANWLTVHARTKADGYTPPA
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCH
YWEKIQPIKDALSINVIANGEIWTNQDAKACQQQSGCEDLMIGRGAVTTPDLTLCIRQNV
HHHHHHHHHHCEEEEEEECCCEECCCCHHHHHHHCCCCHHEECCCCCCCCCHHHHHHCCH
DEALMNWAALIELQQRFLNGEYKTEIGMIGRYKQWLGMMTKAYPEAKQLWDEIKRIKNLD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHH
EILLKLGRSS
HHHHHHCCCC
>Mature Secondary Structure
MKLILAPMEGLTDPIMRDVLTHVGSFDWCVTEFIRVTDSVLPDHVYYTYCPELKNNGKTA
CEEEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
AGTPVHVQFLGNNPDMLAANAEKVVALGAPAIDLNFGCPAKTVNRHRGGSILLDEPDVVH
CCCCEEEEEECCCCCEEECCCCEEEEECCCEEECCCCCCHHHHHHCCCCEEEECCCHHHH
LLVKAVRDAVPAHIPVSAKMRLGYLDENHTMDNAYAIQEAGANWLTVHARTKADGYTPPA
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCH
YWEKIQPIKDALSINVIANGEIWTNQDAKACQQQSGCEDLMIGRGAVTTPDLTLCIRQNV
HHHHHHHHHHCEEEEEEECCCEECCCCHHHHHHHCCCCHHEECCCCCCCCCHHHHHHCCH
DEALMNWAALIELQQRFLNGEYKTEIGMIGRYKQWLGMMTKAYPEAKQLWDEIKRIKNLD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHH
EILLKLGRSS
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]