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The map label for this gene is wax-dgaT

Identifier: 50084045

GI number: 50084045

Start: 819360

End: 820736

Strand: Reverse

Name: wax-dgaT

Synonym: ACIAD0832

Alternate gene names: 50084045

Gene position: 820736-819360 (Counterclockwise)

Preceding gene: 50084046

Following gene: 50084038

Centisome position: 22.81

GC content: 40.16

Gene sequence:

>1377_bases
ATGCGCCCATTACATCCGATTGATTTTATATTCCTGTCACTAGAAAAAAGACAACAGCCTATGCATGTAGGTGGTTTATT
TTTGTTTCAGATTCCTGATAACGCCCCAGACACCTTTATTCAAGATCTGGTGAATGATATCCGGATATCAAAATCAATCC
CTGTTCCACCATTCAACAATAAACTGAATGGGCTTTTTTGGGATGAAGATGAAGAGTTTGATTTAGATCATCATTTTCGT
CATATTGCACTGCCTCATCCTGGTCGTATTCGTGAATTGCTTATTTATATTTCACAAGAGCACAGTACGCTGCTAGATCG
GGCAAAGCCCTTGTGGACCTGCAATATTATTGAAGGAATTGAAGGCAATCGTTTTGCCATGTACTTCAAAATTCACCATG
CGATGGTCGATGGCGTTGCTGGTATGCGGTTAATTGAAAAATCACTCTCCCATGATGTAACAGAAAAAAGTATCGTGCCA
CCTTGGTGTGTTGAGGGAAAACGTGCAAAGCGCTTAAGAGAACCTAAAACAGGTAAAATTAAGAAAATCATGTCTGGTAT
TAAGAGTCAGCTTCAGGCGACACCCACAGTCATTCAAGAGCTTTCTCAGACAGTATTTAAAGATATTGGACGTAATCCTG
ATCATGTTTCAAGCTTTCAGGCGCCTTGTTCTATTTTGAATCAGCGTGTGAGCTCATCGCGACGTTTTGCAGCACAGTCT
TTTGACCTAGATCGTTTTCGTAATATTGCCAAATCGTTGAATGTGACCATTAATGATGTTGTACTAGCGGTATGTTCTGG
TGCATTACGTGCGTATTTGATGAGTCATAATAGTTTGCCTTCAAAACCATTAATTGCCATGGTTCCAGCCTCTATTCGCA
ATGACGATTCAGATGTCAGCAACCGTATTACGATGATTCTGGCAAATTTGGCAACCCACAAAGATGATCCTTTACAACGT
CTTGAAATTATCCGCCGTAGTGTTCAAAACTCAAAGCAACGCTTCAAACGTATGACCAGCGATCAGATTCTAAATTATAG
TGCTGTCGTATATGGCCCTGCAGGACTCAACATAATTTCTGGCATGATGCCAAAACGCCAAGCCTTCAATCTGGTTATTT
CCAATGTGCCTGGCCCAAGAGAGCCACTTTACTGGAATGGTGCCAAACTTGATGCACTCTACCCAGCTTCAATTGTATTA
GACGGTCAAGCATTGAATATTACAATGACCAGTTATTTAGATAAACTTGAAGTTGGTTTGATTGCATGCCGTAATGCATT
GCCAAGAATGCAGAATTTACTGACACATTTAGAAGAAGAAATTCAACTATTTGAAGGCGTAATTGCAAAGCAGGAAGATA
TTAAAACAGCCAATTAA

Upstream 100 bases:

>100_bases
ATGTTGATTAATAACGATATACTCAATACTCGGTTCTATAATTCTAAAAACATAGCTCATAAAGGGTTATTAATATCTTT
GCAGTGAGGCAATCCACGCT

Downstream 100 bases:

>100_bases
AAACAATAAACTTGATTTTTTAATTTATCAGATAAAACTAAAGGGCTAAATTAGCCCTTTAGTTTTTAACAGTACGACAC
TGTTTAAGTAATTGATGACA

Product: bifunctional wax ester synthase/acyl-CoA; diacylglycerol acyltransferase

Products: NA

Alternate protein names: Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFQIPDNAPDTFIQDLVNDIRISKSIPVPPFNNKLNGLFWDEDEEFDLDHHFR
HIALPHPGRIRELLIYISQEHSTLLDRAKPLWTCNIIEGIEGNRFAMYFKIHHAMVDGVAGMRLIEKSLSHDVTEKSIVP
PWCVEGKRAKRLREPKTGKIKKIMSGIKSQLQATPTVIQELSQTVFKDIGRNPDHVSSFQAPCSILNQRVSSSRRFAAQS
FDLDRFRNIAKSLNVTINDVVLAVCSGALRAYLMSHNSLPSKPLIAMVPASIRNDDSDVSNRITMILANLATHKDDPLQR
LEIIRRSVQNSKQRFKRMTSDQILNYSAVVYGPAGLNIISGMMPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASIVL
DGQALNITMTSYLDKLEVGLIACRNALPRMQNLLTHLEEEIQLFEGVIAKQEDIKTAN

Sequences:

>Translated_458_residues
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFQIPDNAPDTFIQDLVNDIRISKSIPVPPFNNKLNGLFWDEDEEFDLDHHFR
HIALPHPGRIRELLIYISQEHSTLLDRAKPLWTCNIIEGIEGNRFAMYFKIHHAMVDGVAGMRLIEKSLSHDVTEKSIVP
PWCVEGKRAKRLREPKTGKIKKIMSGIKSQLQATPTVIQELSQTVFKDIGRNPDHVSSFQAPCSILNQRVSSSRRFAAQS
FDLDRFRNIAKSLNVTINDVVLAVCSGALRAYLMSHNSLPSKPLIAMVPASIRNDDSDVSNRITMILANLATHKDDPLQR
LEIIRRSVQNSKQRFKRMTSDQILNYSAVVYGPAGLNIISGMMPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASIVL
DGQALNITMTSYLDKLEVGLIACRNALPRMQNLLTHLEEEIQLFEGVIAKQEDIKTAN
>Mature_458_residues
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFQIPDNAPDTFIQDLVNDIRISKSIPVPPFNNKLNGLFWDEDEEFDLDHHFR
HIALPHPGRIRELLIYISQEHSTLLDRAKPLWTCNIIEGIEGNRFAMYFKIHHAMVDGVAGMRLIEKSLSHDVTEKSIVP
PWCVEGKRAKRLREPKTGKIKKIMSGIKSQLQATPTVIQELSQTVFKDIGRNPDHVSSFQAPCSILNQRVSSSRRFAAQS
FDLDRFRNIAKSLNVTINDVVLAVCSGALRAYLMSHNSLPSKPLIAMVPASIRNDDSDVSNRITMILANLATHKDDPLQR
LEIIRRSVQNSKQRFKRMTSDQILNYSAVVYGPAGLNIISGMMPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASIVL
DGQALNITMTSYLDKLEVGLIACRNALPRMQNLLTHLEEEIQLFEGVIAKQEDIKTAN

Specific function: Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS us

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WSD_ACIAD (Q8GGG1)

Other databases:

- EMBL:   AF529086
- EMBL:   CR543861
- RefSeq:   YP_045555.1
- ProteinModelPortal:   Q8GGG1
- STRING:   Q8GGG1
- GeneID:   2879218
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0832
- NMPDR:   fig|62977.3.peg.745
- eggNOG:   NOG40303
- HOGENOM:   HBG696081
- OMA:   APRTMLN
- ProtClustDB:   CLSK830521
- BioCyc:   ASP62977:ACIAD0832-MONOMER
- BRENDA:   2.3.1.20
- BRENDA:   2.3.1.75
- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089

EC number: =2.3.1.20; =2.3.1.75

Molecular weight: Translated: 51781; Mature: 51781

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFQIPDNAPDTFIQDLVNDIRISKSIPVPPFNN
CCCCCCHHHHEEEHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
KLNGLFWDEDEEFDLDHHFRHIALPHPGRIRELLIYISQEHSTLLDRAKPLWTCNIIEGI
CCCCCCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEHHHCCC
EGNRFAMYFKIHHAMVDGVAGMRLIEKSLSHDVTEKSIVPPWCVEGKRAKRLREPKTGKI
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHCCCCCCHH
KKIMSGIKSQLQATPTVIQELSQTVFKDIGRNPDHVSSFQAPCSILNQRVSSSRRFAAQS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLDRFRNIAKSLNVTINDVVLAVCSGALRAYLMSHNSLPSKPLIAMVPASIRNDDSDVS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHCCCCHHHH
NRITMILANLATHKDDPLQRLEIIRRSVQNSKQRFKRMTSDQILNYSAVVYGPAGLNIIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHH
GMMPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASIVLDGQALNITMTSYLDKLEVGL
CCCCCHHHHHHHEECCCCCCCCCEECCCEECEECCCEEEECCCEEEEEHHHHHHHHHHHH
IACRNALPRMQNLLTHLEEEIQLFEGVIAKQEDIKTAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFQIPDNAPDTFIQDLVNDIRISKSIPVPPFNN
CCCCCCHHHHEEEHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
KLNGLFWDEDEEFDLDHHFRHIALPHPGRIRELLIYISQEHSTLLDRAKPLWTCNIIEGI
CCCCCCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEHHHCCC
EGNRFAMYFKIHHAMVDGVAGMRLIEKSLSHDVTEKSIVPPWCVEGKRAKRLREPKTGKI
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHCCCCCCHH
KKIMSGIKSQLQATPTVIQELSQTVFKDIGRNPDHVSSFQAPCSILNQRVSSSRRFAAQS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLDRFRNIAKSLNVTINDVVLAVCSGALRAYLMSHNSLPSKPLIAMVPASIRNDDSDVS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHCCCCHHHH
NRITMILANLATHKDDPLQRLEIIRRSVQNSKQRFKRMTSDQILNYSAVVYGPAGLNIIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHH
GMMPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASIVLDGQALNITMTSYLDKLEVGL
CCCCCHHHHHHHEECCCCCCCCCEECCCEECEECCCEEEECCCEEEEEHHHHHHHHHHHH
IACRNALPRMQNLLTHLEEEIQLFEGVIAKQEDIKTAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12502715