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The map label for this gene is proS

Identifier: 50083997

GI number: 50083997

Start: 768210

End: 769922

Strand: Reverse

Name: proS

Synonym: ACIAD0782

Alternate gene names: 50083997

Gene position: 769922-768210 (Counterclockwise)

Preceding gene: 50083998

Following gene: 50083989

Centisome position: 21.39

GC content: 44.02

Gene sequence:

>1713_bases
ATGCGCGCAAGCCGCTTTTTATTTGCAACGTTAAGAGAAACCCCAAATGATGCAGAAGTAATTTCGCATCAGCTCATGTT
ACGAGCTGGGATGATTCGTAAATTGGCTTCAGGTTTATACACTTGGCTGCCGATGGGCGTACGCGTGTTAAATAAAGTTG
AAGCGATTGTACGTGAAGAAATGAATCGCTCAGGCGCGCTTGAAGTCTTTATGCCCGTGACTCAACCCGCAAGTCTTTGG
GAAGAATCAGGCCGTTATGTGCAATATGGCCCAGAACTTTTACGTTTTAAAGATCGTCATGATAATCCATTTGTACTTGG
CCCAACCCATGAAGAAGTGATTACCGATCTAGCACGTAATGAACTGAAAAGTTATAAGCAATTACCCATCAATTTCTATC
AAATTCAAACCAAATTTCGTGATGAAATTCGTCCACGTTTTGGTGTGATGCGTTCACGCGAATTTATCATGAAAGATGCG
TATTCTTTCCATGTTGATCAGGCATCTCTACAAGAAACCTACGACAACATGTACGACACCTACTGCCGTATCTTTACACG
TTTAGGCTTAGATTTCCGCCCTGTACAAGCCGATACTGGCTCAATTGGGGGTTCAGGTTCACATGAGTTTCATGTGCTTG
CAGACAGTGGTGAAGACGATATCGTATTTTCGACTGAATCGGACTATGCAGCAAACGTAGAAAAAGCTGAGGCAGTTTTA
GTCGGAGAGCGTGCAGCCCCTGCGCAAGCATTAAGCATTGTAGATACACCAAATCAAAAAACGATTGCTGATGTTTGTAC
ATTCTTAAACGCTGATCCGAAACAATCAGTAAAAGCGTTGTTAGTGCAGGGCAATGCCGATGAAAAAGGTAATGTCCCTG
TGATTGCGCTATTCTTGCGTGGTGATCATGAGCTTAACGAAATTAAGGCAGAGCATCATCCTTTAATTGCAGCACCTTTA
ACCTTTGCAACTGAAGCGCAACTACAAGAGCTCGGCTTGACCGCAGGTTATGTGGGTCCGCAAGGTTTAGTTGAGAAAGG
CCTGACCGTGATTGTTGATCGCGCAGCGTCAGTGCTGTCAGATTTTATTGCAGGTGCCAACGAAGCCGACAAACATGCAA
CAGGTGTAAACTGGGAACGTGACGCAAAATTTAGCGAAATCTATGATTTGCGTAATGTCGTAGACGGCGATCCATCTCCA
GATGGTCAAGGTGTATTGCAAATCAAACGTGGTATTGAAGTTGGACACATCTTCCAGTTGGGTCAGAAGTATTCTGAAGC
ACTGAACTGTACTGTGTTGGGTGATGACGGTAAGCCATTGGTGGTAACAATGGGCTGTTATGGGATTGGTGTAACGCGCG
TTGTGGCATCTGCCATTGAGCAAAATTACGATGACAAAGGTATTATCTGGCCTTCTGCTATTGCGCCGTTTGAAATTGCC
ATTGTGCCAATGAATGCGCACAAATCACCAAAAACACTTGAAGCTGCCGAAGCACTGTATGCAGAATTGCAAGCACAAGG
CTATGATGTATTGCTTGATGATCGTAATGAACGCCCTGGCGTGAAATTCTCTGATTTGGAGCTAACCGGTATTCCACATC
GTATTGTGATTGGTGAAAAAGGCTTGGATGCAGGAACATTTGAATATAAAGGCCGCACTGATGCTGAGTCTGTTAACTTG
ACTAAAGATGAGCTGTTAGCAAAACTCAAATAA

Upstream 100 bases:

>100_bases
GGTTTTTTATCAAGTGAGGTGAATTATTCATCTCATTTTTTATTATAATCTCTTGTTTTTATCGTGCTCAGTTTGAGAGC
CCACTTTTAGGACAGTTTCC

Downstream 100 bases:

>100_bases
ATTTTAAGATTTTAAAAAAGCAGGTTTGATACCTGCTTTTTTTATCAAAAAAAGCTTAGATATTGATTACAGTGGATTGC
CACAAATATCTTTTGTACCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGVRVLNKVEAIVREEMNRSGALEVFMPVTQPASLW
EESGRYVQYGPELLRFKDRHDNPFVLGPTHEEVITDLARNELKSYKQLPINFYQIQTKFRDEIRPRFGVMRSREFIMKDA
YSFHVDQASLQETYDNMYDTYCRIFTRLGLDFRPVQADTGSIGGSGSHEFHVLADSGEDDIVFSTESDYAANVEKAEAVL
VGERAAPAQALSIVDTPNQKTIADVCTFLNADPKQSVKALLVQGNADEKGNVPVIALFLRGDHELNEIKAEHHPLIAAPL
TFATEAQLQELGLTAGYVGPQGLVEKGLTVIVDRAASVLSDFIAGANEADKHATGVNWERDAKFSEIYDLRNVVDGDPSP
DGQGVLQIKRGIEVGHIFQLGQKYSEALNCTVLGDDGKPLVVTMGCYGIGVTRVVASAIEQNYDDKGIIWPSAIAPFEIA
IVPMNAHKSPKTLEAAEALYAELQAQGYDVLLDDRNERPGVKFSDLELTGIPHRIVIGEKGLDAGTFEYKGRTDAESVNL
TKDELLAKLK

Sequences:

>Translated_570_residues
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGVRVLNKVEAIVREEMNRSGALEVFMPVTQPASLW
EESGRYVQYGPELLRFKDRHDNPFVLGPTHEEVITDLARNELKSYKQLPINFYQIQTKFRDEIRPRFGVMRSREFIMKDA
YSFHVDQASLQETYDNMYDTYCRIFTRLGLDFRPVQADTGSIGGSGSHEFHVLADSGEDDIVFSTESDYAANVEKAEAVL
VGERAAPAQALSIVDTPNQKTIADVCTFLNADPKQSVKALLVQGNADEKGNVPVIALFLRGDHELNEIKAEHHPLIAAPL
TFATEAQLQELGLTAGYVGPQGLVEKGLTVIVDRAASVLSDFIAGANEADKHATGVNWERDAKFSEIYDLRNVVDGDPSP
DGQGVLQIKRGIEVGHIFQLGQKYSEALNCTVLGDDGKPLVVTMGCYGIGVTRVVASAIEQNYDDKGIIWPSAIAPFEIA
IVPMNAHKSPKTLEAAEALYAELQAQGYDVLLDDRNERPGVKFSDLELTGIPHRIVIGEKGLDAGTFEYKGRTDAESVNL
TKDELLAKLK
>Mature_570_residues
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGVRVLNKVEAIVREEMNRSGALEVFMPVTQPASLW
EESGRYVQYGPELLRFKDRHDNPFVLGPTHEEVITDLARNELKSYKQLPINFYQIQTKFRDEIRPRFGVMRSREFIMKDA
YSFHVDQASLQETYDNMYDTYCRIFTRLGLDFRPVQADTGSIGGSGSHEFHVLADSGEDDIVFSTESDYAANVEKAEAVL
VGERAAPAQALSIVDTPNQKTIADVCTFLNADPKQSVKALLVQGNADEKGNVPVIALFLRGDHELNEIKAEHHPLIAAPL
TFATEAQLQELGLTAGYVGPQGLVEKGLTVIVDRAASVLSDFIAGANEADKHATGVNWERDAKFSEIYDLRNVVDGDPSP
DGQGVLQIKRGIEVGHIFQLGQKYSEALNCTVLGDDGKPLVVTMGCYGIGVTRVVASAIEQNYDDKGIIWPSAIAPFEIA
IVPMNAHKSPKTLEAAEALYAELQAQGYDVLLDDRNERPGVKFSDLELTGIPHRIVIGEKGLDAGTFEYKGRTDAESVNL
TKDELLAKLK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=235, Percent_Identity=44.6808510638298, Blast_Score=212, Evalue=7e-55,
Organism=Escherichia coli, GI1786392, Length=557, Percent_Identity=67.3249551166966, Blast_Score=771, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=250, Percent_Identity=38.4, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=38.2022471910112, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=202, Percent_Identity=41.5841584158416, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321808, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=36.734693877551, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ACIAD (Q6FE23)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045507.1
- ProteinModelPortal:   Q6FE23
- SMR:   Q6FE23
- STRING:   Q6FE23
- GeneID:   2880049
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0782
- NMPDR:   fig|62977.3.peg.640
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q6FE23
- ProtClustDB:   PRK09194
- BioCyc:   ASP62977:ACIAD0782-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63003; Mature: 63003

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGVRVLNKVEAIVREE
CCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRSGALEVFMPVTQPASLWEESGRYVQYGPELLRFKDRHDNPFVLGPTHEEVITDLARN
HCCCCCEEEEEECCCCHHHHHHCCCEEEECHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELKSYKQLPINFYQIQTKFRDEIRPRFGVMRSREFIMKDAYSFHVDQASLQETYDNMYDT
HHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
YCRIFTRLGLDFRPVQADTGSIGGSGSHEFHVLADSGEDDIVFSTESDYAANVEKAEAVL
HHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCHHHHHEEE
VGERAAPAQALSIVDTPNQKTIADVCTFLNADPKQSVKALLVQGNADEKGNVPVIALFLR
EECCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHHHHEEEEEECCCCCCCCCCEEEEEEE
GDHELNEIKAEHHPLIAAPLTFATEAQLQELGLTAGYVGPQGLVEKGLTVIVDRAASVLS
CCCHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
DFIAGANEADKHATGVNWERDAKFSEIYDLRNVVDGDPSPDGQGVLQIKRGIEVGHIFQL
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHH
GQKYSEALNCTVLGDDGKPLVVTMGCYGIGVTRVVASAIEQNYDDKGIIWPSAIAPFEIA
HHHHHHHCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IVPMNAHKSPKTLEAAEALYAELQAQGYDVLLDDRNERPGVKFSDLELTGIPHRIVIGEK
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCEEEEEECC
GLDAGTFEYKGRTDAESVNLTKDELLAKLK
CCCCCCEEECCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGVRVLNKVEAIVREE
CCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRSGALEVFMPVTQPASLWEESGRYVQYGPELLRFKDRHDNPFVLGPTHEEVITDLARN
HCCCCCEEEEEECCCCHHHHHHCCCEEEECHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELKSYKQLPINFYQIQTKFRDEIRPRFGVMRSREFIMKDAYSFHVDQASLQETYDNMYDT
HHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
YCRIFTRLGLDFRPVQADTGSIGGSGSHEFHVLADSGEDDIVFSTESDYAANVEKAEAVL
HHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCHHHHHEEE
VGERAAPAQALSIVDTPNQKTIADVCTFLNADPKQSVKALLVQGNADEKGNVPVIALFLR
EECCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHHHHEEEEEECCCCCCCCCCEEEEEEE
GDHELNEIKAEHHPLIAAPLTFATEAQLQELGLTAGYVGPQGLVEKGLTVIVDRAASVLS
CCCHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
DFIAGANEADKHATGVNWERDAKFSEIYDLRNVVDGDPSPDGQGVLQIKRGIEVGHIFQL
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHH
GQKYSEALNCTVLGDDGKPLVVTMGCYGIGVTRVVASAIEQNYDDKGIIWPSAIAPFEIA
HHHHHHHCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IVPMNAHKSPKTLEAAEALYAELQAQGYDVLLDDRNERPGVKFSDLELTGIPHRIVIGEK
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCEEEEEECC
GLDAGTFEYKGRTDAESVNLTKDELLAKLK
CCCCCCEEECCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA