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The map label for this gene is emrA [H]

Identifier: 50083972

GI number: 50083972

Start: 740156

End: 741325

Strand: Reverse

Name: emrA [H]

Synonym: ACIAD0755

Alternate gene names: 50083972

Gene position: 741325-740156 (Counterclockwise)

Preceding gene: 50083973

Following gene: 50083971

Centisome position: 20.6

GC content: 44.44

Gene sequence:

>1170_bases
ATGAGTGACGAGCAAGCACCAAAAGTACCGCCTCAAGCAGCATCAAATGACAATACGCAAGCACAGTCGAATGCCCAGAG
TACAAACTCGTCTACACCTACGCCTTACAAACCTGTTCAGCGGGTGGGGAAACTCATTCCCACCAAAAAATCGACGTTAT
TCATCATGTTCTTTGTTCTTCTGGTTGGGATTGGTCTGATTATGTGGGCATGGCGAATTGGTCCATTTCACACCATGATT
GAACAAACAGATAACAGCTATGTCAAGGGAAAAACCACCGTTTTGTCATCACAGATTAATGGTTATATTAAGGAAGTGCT
GGTGAAGGATTTTGATGATGTCAAACAAGGACAACCGCTTCTTTATATTGATGCCACGACCTACGATCAGAAAGTCACAC
AGGCCGAATCTGCTGTTGCGCAGGCACAAAATACTTTAGCAAACCAGACGCAAACGATTGCGCAGCGTGAGGCGGATATT
ATCGCAGCTCAGGCGCAAGTCGATCAGGCACAGGCAGAGTATCAGCTTTCACTCGATCAACTTAGACGTTATCAGCAGTT
AGGAGATAGCGGTGCAGCCTCAAAGTCTGAACAAGATCAGATTGCAGCCACGGCAAAAAACAATCTGGCTGCTTTAAAGA
AAGCAAAAGCCAATGTCAAAGTTGCAAATGAGGCTTTAAAAACGGCTCAAGTTGCTGAAGTAGGCTTGAATGCTCAAGTA
ACCAGCGCACAGGCACAACTCGATCAGGCCAAAACCACCAAGAATTATAGTGTGATTACAGCACCAATGGATGGTCAGCT
AGGAGAAGTTACACCTCGAATTGGTCAATATGTCGCTGCGGGTTCACAACTTTTATATTTAATTCCCAACCAGACTTGGG
TCATTGCCAATTTCAAGGAAACCCAGATTGCCCATATGAAAATTGGACAGCCTGCAACATTTACCGTAGATGCACTCGAT
CATCAAAAATTTAAAGGGCATATTGAACAGATTTCACCTGCTGCGGGTTCGGAATTTAGTGTACTTAAAGCAGATAATGC
AACTGGTAACTTTACTAAAGTGGTTCAACGAATTTCGGTACGCATTGCGATTGATCCGAATCAGGATGAGCTCAAACGGT
TGCGCCCAGGCATGTCTGTGATTACATCAGTGGATACCTCATCTCGTTGA

Upstream 100 bases:

>100_bases
TCTTTGGGGATTATGTAATATTGCTTGGGGAAAATATCTTGTTTATCGAGAGCGTTTATCGGTTCCCGATTCTTCTAATT
CCTGAATGTAGAGTAAATGT

Downstream 100 bases:

>100_bases
GGATCAACTTCAAGGTTGTCATATAGAAATTAAAGGTTAAACTAGTTGCTCATTCTTATAAGATAAAATGATAGGCTTTT
TTTGAAAAATATCGCTTTTT

Product: multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSDEQAPKVPPQAASNDNTQAQSNAQSTNSSTPTPYKPVQRVGKLIPTKKSTLFIMFFVLLVGIGLIMWAWRIGPFHTMI
EQTDNSYVKGKTTVLSSQINGYIKEVLVKDFDDVKQGQPLLYIDATTYDQKVTQAESAVAQAQNTLANQTQTIAQREADI
IAAQAQVDQAQAEYQLSLDQLRRYQQLGDSGAASKSEQDQIAATAKNNLAALKKAKANVKVANEALKTAQVAEVGLNAQV
TSAQAQLDQAKTTKNYSVITAPMDGQLGEVTPRIGQYVAAGSQLLYLIPNQTWVIANFKETQIAHMKIGQPATFTVDALD
HQKFKGHIEQISPAAGSEFSVLKADNATGNFTKVVQRISVRIAIDPNQDELKRLRPGMSVITSVDTSSR

Sequences:

>Translated_389_residues
MSDEQAPKVPPQAASNDNTQAQSNAQSTNSSTPTPYKPVQRVGKLIPTKKSTLFIMFFVLLVGIGLIMWAWRIGPFHTMI
EQTDNSYVKGKTTVLSSQINGYIKEVLVKDFDDVKQGQPLLYIDATTYDQKVTQAESAVAQAQNTLANQTQTIAQREADI
IAAQAQVDQAQAEYQLSLDQLRRYQQLGDSGAASKSEQDQIAATAKNNLAALKKAKANVKVANEALKTAQVAEVGLNAQV
TSAQAQLDQAKTTKNYSVITAPMDGQLGEVTPRIGQYVAAGSQLLYLIPNQTWVIANFKETQIAHMKIGQPATFTVDALD
HQKFKGHIEQISPAAGSEFSVLKADNATGNFTKVVQRISVRIAIDPNQDELKRLRPGMSVITSVDTSSR
>Mature_388_residues
SDEQAPKVPPQAASNDNTQAQSNAQSTNSSTPTPYKPVQRVGKLIPTKKSTLFIMFFVLLVGIGLIMWAWRIGPFHTMIE
QTDNSYVKGKTTVLSSQINGYIKEVLVKDFDDVKQGQPLLYIDATTYDQKVTQAESAVAQAQNTLANQTQTIAQREADII
AAQAQVDQAQAEYQLSLDQLRRYQQLGDSGAASKSEQDQIAATAKNNLAALKKAKANVKVANEALKTAQVAEVGLNAQVT
SAQAQLDQAKTTKNYSVITAPMDGQLGEVTPRIGQYVAAGSQLLYLIPNQTWVIANFKETQIAHMKIGQPATFTVDALDH
QKFKGHIEQISPAAGSEFSVLKADNATGNFTKVVQRISVRIAIDPNQDELKRLRPGMSVITSVDTSSR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=373, Percent_Identity=32.7077747989276, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788711, Length=340, Percent_Identity=31.7647058823529, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1790519, Length=330, Percent_Identity=25.1515151515152, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI87081951, Length=315, Percent_Identity=24.7619047619048, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081796, Length=298, Percent_Identity=28.5234899328859, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790024, Length=283, Percent_Identity=27.9151943462898, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789900, Length=301, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790012, Length=243, Percent_Identity=26.7489711934156, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42307; Mature: 42176

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEECCCHHHHHHHCCCHHHEECCCCCCC
>Mature Secondary Structure 
SDEQAPKVPPQAASNDNTQAQSNAQSTNSSTPTPYKPVQRVGKLIPTKKSTLFIMFFVL
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
LVGIGLIMWAWRIGPFHTMIEQTDNSYVKGKTTVLSSQINGYIKEVLVKDFDDVKQGQPL
HHHHHHHHHHHHCCHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LYIDATTYDQKVTQAESAVAQAQNTLANQTQTIAQREADIIAAQAQVDQAQAEYQLSLDQ
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRYQQLGDSGAASKSEQDQIAATAKNNLAALKKAKANVKVANEALKTAQVAEVGLNAQV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEE
TSAQAQLDQAKTTKNYSVITAPMDGQLGEVTPRIGQYVAAGSQLLYLIPNQTWVIANFKE
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEEEECCC
TQIAHMKIGQPATFTVDALDHQKFKGHIEQISPAAGSEFSVLKADNATGNFTKVVQRISV
CEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHEE
RIAIDPNQDELKRLRPGMSVITSVDTSSR
EEEECCCHHHHHHHCCCHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]