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The map label for this gene is yeiE [H]

Identifier: 50083964

GI number: 50083964

Start: 732464

End: 733351

Strand: Reverse

Name: yeiE [H]

Synonym: ACIAD0746

Alternate gene names: 50083964

Gene position: 733351-732464 (Counterclockwise)

Preceding gene: 50083966

Following gene: 50083948

Centisome position: 20.38

GC content: 42.91

Gene sequence:

>888_bases
ATGCGCATGACATTACGTCAGTTGGCTGTTTTTGTGGCAGTCGCTCAAGAAGGTACTGTAACAAAAGCAAGCGATGCTGT
TCGCCTCACACAAAGTGCAGCGAGTATGGCTTTGGCTGATCTTGAGGATGGGCTTGGAGCACCTTTATTTGATCGTTTAG
GGAAGCGTTTACAGCTCAATGATCTTGGTCGTTTTCTATTACCTCAAGCCTTGGAAATATTAGGCCGCTGTGAAGCCTTT
GAACAGGCAGCCAAAGGTGAATTACAAAGTATTGATTTACGCCTAGGTGCAACTTTAACGATCAGTGACTATCTGATTCC
AGACCTGATGGCAGATTTTCTTCAAATTCATCCACAAGCACATTTACAGCTTCAAGTGGGTAATACCCGCCAAATGATTG
AAGCAGTGAATCAGTTTCAGCTAGATCTGGCACTCATTGAAGGCTCTTGTCATTTGCCTCAATTACAATGTATTCACTGG
CGTAATGATGAACTTGCTGTATGTTGTGCGCCTGATCATCCACTCGCAAAACTTGGCCGCCCCCTCACGGCGCAAGATTT
CTTAAATGTTGAATGGATTTTGCGTGAAGAAGGCTCTGGGACACGTGAAGTGTTTGACAATGCTATTTTGCAGGATGTAC
CTGATGCCAATATTCGTTTGACTTTGGGGCATAATGAAGCGATTTTAAAAATTGTTGCTGGGGGACTCGGTATGTCCTGT
ATTTCCAGACTAGCGATCGAACCTTTAATTGAAAAAGGTCAATTGGTGATTTTAGAAACTCCTTTTTGGGAACTTACCCG
CCCTTTACATCTATTAGTCCATCGTCAAAAATATCAGGGACCTGGTTTAAAGGCCTTTATGAACTTCTGTGAAAATCGAG
TGAATTAA

Upstream 100 bases:

>100_bases
TTTTTCAATAGACATAATTTCAACGTGGCACGAAATGGTGTGCTCATGTTAGCATGACAGCTCAAATTATTAATACTTTT
TAAAGGGTAAGGCTTCGTTC

Downstream 100 bases:

>100_bases
TTTTTTACGCGATTTCATTAATCTGAATAAAAAACTCTTTCAATTGAAAGAGTTTTTTATTTATTCCTTCATTTAGAAAT
CAAATGTTACACCCAGTTTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRMTLRQLAVFVAVAQEGTVTKASDAVRLTQSAASMALADLEDGLGAPLFDRLGKRLQLNDLGRFLLPQALEILGRCEAF
EQAAKGELQSIDLRLGATLTISDYLIPDLMADFLQIHPQAHLQLQVGNTRQMIEAVNQFQLDLALIEGSCHLPQLQCIHW
RNDELAVCCAPDHPLAKLGRPLTAQDFLNVEWILREEGSGTREVFDNAILQDVPDANIRLTLGHNEAILKIVAGGLGMSC
ISRLAIEPLIEKGQLVILETPFWELTRPLHLLVHRQKYQGPGLKAFMNFCENRVN

Sequences:

>Translated_295_residues
MRMTLRQLAVFVAVAQEGTVTKASDAVRLTQSAASMALADLEDGLGAPLFDRLGKRLQLNDLGRFLLPQALEILGRCEAF
EQAAKGELQSIDLRLGATLTISDYLIPDLMADFLQIHPQAHLQLQVGNTRQMIEAVNQFQLDLALIEGSCHLPQLQCIHW
RNDELAVCCAPDHPLAKLGRPLTAQDFLNVEWILREEGSGTREVFDNAILQDVPDANIRLTLGHNEAILKIVAGGLGMSC
ISRLAIEPLIEKGQLVILETPFWELTRPLHLLVHRQKYQGPGLKAFMNFCENRVN
>Mature_295_residues
MRMTLRQLAVFVAVAQEGTVTKASDAVRLTQSAASMALADLEDGLGAPLFDRLGKRLQLNDLGRFLLPQALEILGRCEAF
EQAAKGELQSIDLRLGATLTISDYLIPDLMADFLQIHPQAHLQLQVGNTRQMIEAVNQFQLDLALIEGSCHLPQLQCIHW
RNDELAVCCAPDHPLAKLGRPLTAQDFLNVEWILREEGSGTREVFDNAILQDVPDANIRLTLGHNEAILKIVAGGLGMSC
ISRLAIEPLIEKGQLVILETPFWELTRPLHLLVHRQKYQGPGLKAFMNFCENRVN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=41.2371134020619, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=27.8523489932886, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=27.3037542662116, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=27.734375, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082132, Length=295, Percent_Identity=24.7457627118644, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790399, Length=261, Percent_Identity=23.7547892720307, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=181, Percent_Identity=29.8342541436464, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788887, Length=175, Percent_Identity=29.7142857142857, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786401, Length=316, Percent_Identity=23.1012658227848, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693105, Length=248, Percent_Identity=21.7741935483871, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787806, Length=291, Percent_Identity=24.7422680412371, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790208, Length=300, Percent_Identity=23.3333333333333, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081904, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787879, Length=261, Percent_Identity=25.6704980842912, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32755; Mature: 32755

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMTLRQLAVFVAVAQEGTVTKASDAVRLTQSAASMALADLEDGLGAPLFDRLGKRLQLN
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHH
DLGRFLLPQALEILGRCEAFEQAAKGELQSIDLRLGATLTISDYLIPDLMADFLQIHPQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEHHHHHHHHHHHHHHHCCCE
HLQLQVGNTRQMIEAVNQFQLDLALIEGSCHLPQLQCIHWRNDELAVCCAPDHPLAKLGR
EEEEEECCHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCEEEEECCCCCHHHHCC
PLTAQDFLNVEWILREEGSGTREVFDNAILQDVPDANIRLTLGHNEAILKIVAGGLGMSC
CCCHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHCCCCHHH
ISRLAIEPLIEKGQLVILETPFWELTRPLHLLVHRQKYQGPGLKAFMNFCENRVN
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRMTLRQLAVFVAVAQEGTVTKASDAVRLTQSAASMALADLEDGLGAPLFDRLGKRLQLN
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHH
DLGRFLLPQALEILGRCEAFEQAAKGELQSIDLRLGATLTISDYLIPDLMADFLQIHPQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEHHHHHHHHHHHHHHHCCCE
HLQLQVGNTRQMIEAVNQFQLDLALIEGSCHLPQLQCIHWRNDELAVCCAPDHPLAKLGR
EEEEEECCHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCEEEEECCCCCHHHHCC
PLTAQDFLNVEWILREEGSGTREVFDNAILQDVPDANIRLTLGHNEAILKIVAGGLGMSC
CCCHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHCCCCHHH
ISRLAIEPLIEKGQLVILETPFWELTRPLHLLVHRQKYQGPGLKAFMNFCENRVN
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]