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The map label for this gene is nuoF [H]

Identifier: 50083953

GI number: 50083953

Start: 717897

End: 719228

Strand: Direct

Name: nuoF [H]

Synonym: ACIAD0735

Alternate gene names: 50083953

Gene position: 717897-719228 (Clockwise)

Preceding gene: 50083952

Following gene: 50083954

Centisome position: 19.95

GC content: 46.62

Gene sequence:

>1332_bases
ATGAATACTGAACCAAAACCGATTTATGGCGACGGAAACCCTGAAACTCATCCGCTGACTTGGCGTTTGAGTAAGCAGGA
GCATGTACGTAGTGCTGATGATTACGAAGCACTAGACGGTTATGCAGGTTTTAAAAAAGCCATTGGCATGACACCTGCTG
AAGTCCTTGATGTCGTAAAAGCCGCAACAGTCAAAGGCCGTGGTGGTGCTGGTTTCCCTGCCGGGATCAAATGGTCATTG
ATGGCGCCAAATGATGGTGGTCCTCGCTACCTGATTTGTAATGCCGATGAAATGGAACCAGGGACTTTTAAAGACCGTCT
ATTGATGGAAAAGTTACCTCACCAGCTGATTGAAGGTATGTTGATTGCAGCATATACACTTGAAGCGACTCAGGGTTACA
TCTTTATCCGTGGTGAATATATTGAAGCTGCCCAGTACTTAAATGAAGCACTGGAACAGGCTCGTGCCAAAGGTTATCTT
GGCGATAATATTCTTGGAACAGGCTGGAATTTCGAGCTTTACGTCCATACGGGTGCGGGGCGTTATATTTGTGGTGAAGA
AACTGCATTGATTAACTCACTGGAAGGCCGTCGTGCCAACCCACGTACCAAACCACCATTTCCACAGGTTGCAGGTGCTT
GGGGTCGTCCAACCATCGTCAATAACGTAGAAACCTATAATAACTTACCAGCGATCATGTTACGTGGTCCTGAGTGGTAT
ATCGGATTGTCTGCTGGCAAGTCAAAAGACCCAGGTACCAAGATTTATGGTGCATCGGGTAAAGTTAAATTCCCAGGTTT
ATGGGAGCTTCCATTTGGTACAACTGCGCGCGAAGTGATTGAAGAGCATGCAGGTGGTATGCGTGATGGATTAAAGCTGA
AAGCATGGTTACCAGGTGGTGCATCAACAGACTTTTTGGCTGCTGAGCACATTGATCTGCCTATGGATTCAGAAAGCATT
ATGAAGGCGGGTTCTCGTTTGGGAACATGCTTGCTGATGGTGGTGGATGAAACCCAATGTATGGTTTCAGCAACACGTAA
TCTGGAAGAATTCTTTGCACGTGAATCATGTGGTTGGTGTACACCCTGTCGTGATGGTTTGCCTTGGGCTGTGAAAGCAT
TAAAAGCGCTCGAGAATGGTGAAGGGAAGATGGAAGATATTCAGCATCTTCAGGAACTGACTCGAAAGTTATGGATTGGT
AAGACTTTCTGTGCTCACGCACCAGGTGCGATGGAGCCACTGATGGGCGCACTCAAATATTTCCGTAGCGAGTTTGAAGC
GAAAGTGGCAAAAAGCCCAGTCGTTCAAACTAGCCACGTAGCACAAGCTTAA

Upstream 100 bases:

>100_bases
GTTTAGGAAATTGCGATAAAGGACCAACCTTAATGATTGATGAAGATACACATGGCTTAGTTGATGTATCTTCGGTCAAA
CAGTTATTGGAGAAGTATGT

Downstream 100 bases:

>100_bases
GGAATTCGACTATGGCTACAATTCATGTCGATGGAAAATCTTACGAAGTCGATGGATCAGAAAACCTGTTGCAGGCCTGT
TTATCACTTGGTCTTGATAT

Product: NADH dehydrogenase I chain F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MNTEPKPIYGDGNPETHPLTWRLSKQEHVRSADDYEALDGYAGFKKAIGMTPAEVLDVVKAATVKGRGGAGFPAGIKWSL
MAPNDGGPRYLICNADEMEPGTFKDRLLMEKLPHQLIEGMLIAAYTLEATQGYIFIRGEYIEAAQYLNEALEQARAKGYL
GDNILGTGWNFELYVHTGAGRYICGEETALINSLEGRRANPRTKPPFPQVAGAWGRPTIVNNVETYNNLPAIMLRGPEWY
IGLSAGKSKDPGTKIYGASGKVKFPGLWELPFGTTAREVIEEHAGGMRDGLKLKAWLPGGASTDFLAAEHIDLPMDSESI
MKAGSRLGTCLLMVVDETQCMVSATRNLEEFFARESCGWCTPCRDGLPWAVKALKALENGEGKMEDIQHLQELTRKLWIG
KTFCAHAPGAMEPLMGALKYFRSEFEAKVAKSPVVQTSHVAQA

Sequences:

>Translated_443_residues
MNTEPKPIYGDGNPETHPLTWRLSKQEHVRSADDYEALDGYAGFKKAIGMTPAEVLDVVKAATVKGRGGAGFPAGIKWSL
MAPNDGGPRYLICNADEMEPGTFKDRLLMEKLPHQLIEGMLIAAYTLEATQGYIFIRGEYIEAAQYLNEALEQARAKGYL
GDNILGTGWNFELYVHTGAGRYICGEETALINSLEGRRANPRTKPPFPQVAGAWGRPTIVNNVETYNNLPAIMLRGPEWY
IGLSAGKSKDPGTKIYGASGKVKFPGLWELPFGTTAREVIEEHAGGMRDGLKLKAWLPGGASTDFLAAEHIDLPMDSESI
MKAGSRLGTCLLMVVDETQCMVSATRNLEEFFARESCGWCTPCRDGLPWAVKALKALENGEGKMEDIQHLQELTRKLWIG
KTFCAHAPGAMEPLMGALKYFRSEFEAKVAKSPVVQTSHVAQA
>Mature_443_residues
MNTEPKPIYGDGNPETHPLTWRLSKQEHVRSADDYEALDGYAGFKKAIGMTPAEVLDVVKAATVKGRGGAGFPAGIKWSL
MAPNDGGPRYLICNADEMEPGTFKDRLLMEKLPHQLIEGMLIAAYTLEATQGYIFIRGEYIEAAQYLNEALEQARAKGYL
GDNILGTGWNFELYVHTGAGRYICGEETALINSLEGRRANPRTKPPFPQVAGAWGRPTIVNNVETYNNLPAIMLRGPEWY
IGLSAGKSKDPGTKIYGASGKVKFPGLWELPFGTTAREVIEEHAGGMRDGLKLKAWLPGGASTDFLAAEHIDLPMDSESI
MKAGSRLGTCLLMVVDETQCMVSATRNLEEFFARESCGWCTPCRDGLPWAVKALKALENGEGKMEDIQHLQELTRKLWIG
KTFCAHAPGAMEPLMGALKYFRSEFEAKVAKSPVVQTSHVAQA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=383, Percent_Identity=41.77545691906, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI20149568, Length=383, Percent_Identity=41.77545691906, Blast_Score=307, Evalue=2e-83,
Organism=Escherichia coli, GI1788620, Length=429, Percent_Identity=66.2004662004662, Blast_Score=576, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI17531727, Length=380, Percent_Identity=43.421052631579, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI45550452, Length=380, Percent_Identity=43.6842105263158, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI19920794, Length=378, Percent_Identity=42.0634920634921, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24653819, Length=386, Percent_Identity=34.1968911917098, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24653817, Length=348, Percent_Identity=34.1954022988506, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48533; Mature: 48533

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEPKPIYGDGNPETHPLTWRLSKQEHVRSADDYEALDGYAGFKKAIGMTPAEVLDVVK
CCCCCCCEECCCCCCCCCEEEEECHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AATVKGRGGAGFPAGIKWSLMAPNDGGPRYLICNADEMEPGTFKDRLLMEKLPHQLIEGM
HHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
LIAAYTLEATQGYIFIRGEYIEAAQYLNEALEQARAKGYLGDNILGTGWNFELYVHTGAG
HHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEECCCC
RYICGEETALINSLEGRRANPRTKPPFPQVAGAWGRPTIVNNVETYNNLPAIMLRGPEWY
CEEECCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCEECCCHHHCCCCEEEEECCCEE
IGLSAGKSKDPGTKIYGASGKVKFPGLWELPFGTTAREVIEEHAGGMRDGLKLKAWLPGG
EEECCCCCCCCCCEEECCCCCEECCEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCC
ASTDFLAAEHIDLPMDSESIMKAGSRLGTCLLMVVDETQCMVSATRNLEEFFARESCGWC
CCCCCEEHHCCCCCCCHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
TPCRDGLPWAVKALKALENGEGKMEDIQHLQELTRKLWIGKTFCAHAPGAMEPLMGALKY
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHH
FRSEFEAKVAKSPVVQTSHVAQA
HHHHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MNTEPKPIYGDGNPETHPLTWRLSKQEHVRSADDYEALDGYAGFKKAIGMTPAEVLDVVK
CCCCCCCEECCCCCCCCCEEEEECHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AATVKGRGGAGFPAGIKWSLMAPNDGGPRYLICNADEMEPGTFKDRLLMEKLPHQLIEGM
HHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
LIAAYTLEATQGYIFIRGEYIEAAQYLNEALEQARAKGYLGDNILGTGWNFELYVHTGAG
HHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEECCCC
RYICGEETALINSLEGRRANPRTKPPFPQVAGAWGRPTIVNNVETYNNLPAIMLRGPEWY
CEEECCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCEECCCHHHCCCCEEEEECCCEE
IGLSAGKSKDPGTKIYGASGKVKFPGLWELPFGTTAREVIEEHAGGMRDGLKLKAWLPGG
EEECCCCCCCCCCEEECCCCCEECCEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCC
ASTDFLAAEHIDLPMDSESIMKAGSRLGTCLLMVVDETQCMVSATRNLEEFFARESCGWC
CCCCCEEHHCCCCCCCHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
TPCRDGLPWAVKALKALENGEGKMEDIQHLQELTRKLWIGKTFCAHAPGAMEPLMGALKY
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHH
FRSEFEAKVAKSPVVQTSHVAQA
HHHHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]