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The map label for this gene is thlA [H]

Identifier: 50083920

GI number: 50083920

Start: 682261

End: 683436

Strand: Reverse

Name: thlA [H]

Synonym: ACIAD0694

Alternate gene names: 50083920

Gene position: 683436-682261 (Counterclockwise)

Preceding gene: 50083921

Following gene: 50083919

Centisome position: 18.99

GC content: 43.62

Gene sequence:

>1176_bases
ATGACTGAGATCGTGATTGTAAATGGCGCACGTACAGCGATGGGCGGCTTTCAAGGTGCTTTAGCTCCGCTGACTGCCAC
TGATTTGGGTGCAGTTACCATTAAAGAAGCGATTTATCGTTCGGGTTTACAACCTACAGATATCGAAGAAGTCATTATGG
GATGTGTATTGCCAGCAGGTCTAAAGCAAGGCCCTGCACGCCAAGCTATGCGTAAGGCTGGACTGCCTGATACAACAGGT
GCTGTCACGATCAATAAATTGTGTGGTTCTGGCATGAAAGCAGTGATGCAAGCTGTAGATATGATTAAGGCGGGTAGTGC
AGAAATTGTGATTGCTGGTGGTATGGAGTCGATGAGTAACGCGCCTTATGTTTTGCCTAAAGCACGTTCTGGTTATCGTA
TGGGACATGGTGAAATTAAGGATCATATGTTTTTAGACGGTTTGGAAGATGCCGAAACAGGTCGTCTAATGGGTTCTTTT
GCACAAGATATGGCGAATACACGTGGCTATACCCGTGAACAAATGGATGACTTTGCCATTCGCTCTTTAAAGCGTGCCCA
AAATGCGATTACAGAAGGTTATTTTAAAGCAGAAATTGTTCCAGTCACCGTGTCATCACGTAAAGGCGATGTGATCATTG
AACAGGACGAACAACCTCTCAATGCAAAAATTGATAAAATTCCTTCTTTAAAACCTGCTTTTGCCAAAGATGGTACCATT
ACTGCTGCAAACTCAAGCTCAATTTCAGATGGTGCTTCTGCTCTTGTATTAACATCTATAGAAAATGCTGATCAGCGCGG
CTTAAAACCACTGGCCAAAGTTATTGCAACCGCATCTAACTCTCAGCATCCTTCAGAGTTTACGATTGCCCCTGTAGGAG
CAATTGGCAAAGTATTAAAAAAATCGGGCTGGAATGCTCAAGAAGTTGATTTATGGGAAATCAATGAAGCTTTTGCCATG
GTCACTATGTGTGCTATCGATGATTTTGAACTAGATGCTGAGAAAGTAAACATCCACGGTGGGGCATGTGCCTTAGGTCA
TCCTGTTGGATCAACAGGCTCACGTATCATCTTGTCTTTGATTCATGCATTACAACGCACAGGCGGTAAAAAAGGCATAG
CAGCCCTATGTATTGGCGGTGGTGAAGCAACAGCAGTAGCTATCGAATTAATTTAG

Upstream 100 bases:

>100_bases
CATAATCAAGTCGTTCCCTCACCTGCAATGGCTATTTTTTTGTCTATACTGCTGAAATCAGTGTGAAGATTTAGACCACA
ATATTCTAGAGAGGACAACA

Downstream 100 bases:

>100_bases
TATTTATGCATCTATAAATCCCGACAAGTCGGGATTTTTTATGATAAGAAACAATATAATTTTTGCTTATTTTTTACTCA
AAAATATTTTACAGTGGTTA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTEIVIVNGARTAMGGFQGALAPLTATDLGAVTIKEAIYRSGLQPTDIEEVIMGCVLPAGLKQGPARQAMRKAGLPDTTG
AVTINKLCGSGMKAVMQAVDMIKAGSAEIVIAGGMESMSNAPYVLPKARSGYRMGHGEIKDHMFLDGLEDAETGRLMGSF
AQDMANTRGYTREQMDDFAIRSLKRAQNAITEGYFKAEIVPVTVSSRKGDVIIEQDEQPLNAKIDKIPSLKPAFAKDGTI
TAANSSSISDGASALVLTSIENADQRGLKPLAKVIATASNSQHPSEFTIAPVGAIGKVLKKSGWNAQEVDLWEINEAFAM
VTMCAIDDFELDAEKVNIHGGACALGHPVGSTGSRIILSLIHALQRTGGKKGIAALCIGGGEATAVAIELI

Sequences:

>Translated_391_residues
MTEIVIVNGARTAMGGFQGALAPLTATDLGAVTIKEAIYRSGLQPTDIEEVIMGCVLPAGLKQGPARQAMRKAGLPDTTG
AVTINKLCGSGMKAVMQAVDMIKAGSAEIVIAGGMESMSNAPYVLPKARSGYRMGHGEIKDHMFLDGLEDAETGRLMGSF
AQDMANTRGYTREQMDDFAIRSLKRAQNAITEGYFKAEIVPVTVSSRKGDVIIEQDEQPLNAKIDKIPSLKPAFAKDGTI
TAANSSSISDGASALVLTSIENADQRGLKPLAKVIATASNSQHPSEFTIAPVGAIGKVLKKSGWNAQEVDLWEINEAFAM
VTMCAIDDFELDAEKVNIHGGACALGHPVGSTGSRIILSLIHALQRTGGKKGIAALCIGGGEATAVAIELI
>Mature_390_residues
TEIVIVNGARTAMGGFQGALAPLTATDLGAVTIKEAIYRSGLQPTDIEEVIMGCVLPAGLKQGPARQAMRKAGLPDTTGA
VTINKLCGSGMKAVMQAVDMIKAGSAEIVIAGGMESMSNAPYVLPKARSGYRMGHGEIKDHMFLDGLEDAETGRLMGSFA
QDMANTRGYTREQMDDFAIRSLKRAQNAITEGYFKAEIVPVTVSSRKGDVIIEQDEQPLNAKIDKIPSLKPAFAKDGTIT
AANSSSISDGASALVLTSIENADQRGLKPLAKVIATASNSQHPSEFTIAPVGAIGKVLKKSGWNAQEVDLWEINEAFAMV
TMCAIDDFELDAEKVNIHGGACALGHPVGSTGSRIILSLIHALQRTGGKKGIAALCIGGGEATAVAIELI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=45.2442159383033, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI4557237, Length=391, Percent_Identity=42.4552429667519, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=36.7609254498715, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=34.5, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=29.7169811320755, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=42.6966292134831, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788554, Length=391, Percent_Identity=47.3145780051151, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=43.3333333333333, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=37.3134328358209, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=35.9605911330049, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=29.5121951219512, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=44.7300771208226, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25147385, Length=384, Percent_Identity=39.84375, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=38.974358974359, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=33.2474226804124, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=30.4245283018868, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=394, Percent_Identity=43.9086294416244, Blast_Score=315, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=33.587786259542, Blast_Score=179, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24655093, Length=390, Percent_Identity=45.1282051282051, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=44.5012787723785, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=37.0558375634518, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=30.0947867298578, Blast_Score=171, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40955; Mature: 40823

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIVIVNGARTAMGGFQGALAPLTATDLGAVTIKEAIYRSGLQPTDIEEVIMGCVLPAG
CCEEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
LKQGPARQAMRKAGLPDTTGAVTINKLCGSGMKAVMQAVDMIKAGSAEIVIAGGMESMSN
CCCCHHHHHHHHCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
APYVLPKARSGYRMGHGEIKDHMFLDGLEDAETGRLMGSFAQDMANTRGYTREQMDDFAI
CCEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RSLKRAQNAITEGYFKAEIVPVTVSSRKGDVIIEQDEQPLNAKIDKIPSLKPAFAKDGTI
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCEE
TAANSSSISDGASALVLTSIENADQRGLKPLAKVIATASNSQHPSEFTIAPVGAIGKVLK
EECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHH
KSGWNAQEVDLWEINEAFAMVTMCAIDDFELDAEKVNIHGGACALGHPVGSTGSRIILSL
HCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHH
IHALQRTGGKKGIAALCIGGGEATAVAIELI
HHHHHHCCCCCCEEEEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TEIVIVNGARTAMGGFQGALAPLTATDLGAVTIKEAIYRSGLQPTDIEEVIMGCVLPAG
CEEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
LKQGPARQAMRKAGLPDTTGAVTINKLCGSGMKAVMQAVDMIKAGSAEIVIAGGMESMSN
CCCCHHHHHHHHCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
APYVLPKARSGYRMGHGEIKDHMFLDGLEDAETGRLMGSFAQDMANTRGYTREQMDDFAI
CCEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RSLKRAQNAITEGYFKAEIVPVTVSSRKGDVIIEQDEQPLNAKIDKIPSLKPAFAKDGTI
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCEE
TAANSSSISDGASALVLTSIENADQRGLKPLAKVIATASNSQHPSEFTIAPVGAIGKVLK
EECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHH
KSGWNAQEVDLWEINEAFAMVTMCAIDDFELDAEKVNIHGGACALGHPVGSTGSRIILSL
HCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHH
IHALQRTGGKKGIAALCIGGGEATAVAIELI
HHHHHHCCCCCCEEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]