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The map label for this gene is fumA [H]

Identifier: 50083776

GI number: 50083776

Start: 525159

End: 526685

Strand: Reverse

Name: fumA [H]

Synonym: ACIAD0538

Alternate gene names: 50083776

Gene position: 526685-525159 (Counterclockwise)

Preceding gene: 50083778

Following gene: 50083775

Centisome position: 14.64

GC content: 44.6

Gene sequence:

>1527_bases
ATGACCACAATTATCAAACAAGAAGACTTGATCACTTCGGTCAAGGATGCCCTTCAGTTCATTTCCTACTATCATCCGCA
AGATTTTATTCAGGCCATGAGCCGTGCGTACGATCGTGAAGCGAACCAAGCTGCAAAGGATGCAATCGCACAAATTCTCA
TCAACTCTCGTATGTGTGCAGAAGGTCATCGCCCAATCTGTCAAGATACAGGTATCGTCAACGTATTTGTTGATGTCGGT
CTAGATGTTAAGTTCGATCTTAGCATGAGCCTAGATGATGCGATTAATGAAGGTGTACGCCAAGGTTATCTTGAAAATAC
CAACGTACTCCGTGCTTCTGTGCTTGCAGATCCAGCCTTTGGCCGTAAAAATACCAAAGACAACACCCCCGCTGTAATCC
ATTATAAACTTGTTCCAGGTAACAAAGTTGATATTACAGTAGCCGCTAAAGGCGGTGGTTCTGAAAATAAATCTAAACTT
GCCATGTTGAATCCGTCTGATTCAATTGTGGACTGGGTACTGAAAACTGTTCCAACTATGGGCGCTGGCTGGTGTCCTCC
AGGTATGCTGGGTATCGGGATTGGTGGTACTGCTGAAAAAGCCATGATGCTTGCCAAAGAAGCATTGATGGAAGAAATCA
ACATGGACGAATTACTTCGTCGCGGGCCAGAAAACAAAATTGAAGAACTTCGTATCGAAATCTTCGAGAAAGTGAATGCG
CTTGGTATTGGTGCACAAGGTCTTGGCGGTTTGACTACAGTTCTCGATATCAAAATTAAAGATTATCCATGTCATGCGGC
AGGCAAACCAGTGGGCATGATTCCAAACTGTGCTGCGACTCGTCATGCTCATTTTCAGTTAGATGGTTCAGGCCCTGCAC
ATATTCAAGCACCTAAACTTGAAGATTACCCAGAAGTGACGTGGGATTCATCTAAATCAAAACGCGTAAACCTTGATACC
ATCACTCAAGAAGAAATGAACTCTTGGCAGCCAGGTGATACCTTGTTGCTCAGTGGCACCATGTACACTGGTCGTGATGC
AGCGCATAAACGTCTGGTAGAAATGATCGATAATGGTGAAGAGCTTCCTGTCGATCTTAAAGGTAAGTTTATTTACTACG
TGGGTCCAGTCGATCCTGTAGGTGATGAAGTGGTTGGACCTGCTGGCCCAACAACAGCAACGCGTATGGACAAATTCACA
CGTAAAGTGCTTGAGCATACGGGTCTGTTTGGCATGATTGGTAAAGCAGATCGTGGCCCAACCGCGATTGAAGCGATTAA
AGATAATCAAGCAACGTATCTCATGGCAGTCGGTGGTGCAGCATACCTTGTATCTAAGGCAGTGCGTGAAGCCGAAGTGG
TTGCATTTGCTGATCTAGGTATGGAAGCTATCTACAAATTTGTAGTCGAAGACATGCCAGTATCCGTTGCAGTTGATGTT
AACGGTACATCGGTACATGCAATTGCACCCAAAATCTGGCAAGAAAAGATTGGTAAAATTCCAGTGATTGATGCCACTGC
AGTTTAA

Upstream 100 bases:

>100_bases
TCGAGCTTTAGCCAAAATCTCGCCAAAACTCCCCACAGCCTGATCGTTTTGTCATATAATTTAGCCCGCTAGCTAACTGC
CCGCTCAAGAGATTTTTGGT

Downstream 100 bases:

>100_bases
GCGATAAAAGCGCCTACGGGCGCTTTTTTTTGCCCAAAAGTGTTAATGTGCACAAACACCACAAAAACACAAAAGTAAAT
CCCACTCAATCCAGTAGAAT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTTIIKQEDLITSVKDALQFISYYHPQDFIQAMSRAYDREANQAAKDAIAQILINSRMCAEGHRPICQDTGIVNVFVDVG
LDVKFDLSMSLDDAINEGVRQGYLENTNVLRASVLADPAFGRKNTKDNTPAVIHYKLVPGNKVDITVAAKGGGSENKSKL
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMMLAKEALMEEINMDELLRRGPENKIEELRIEIFEKVNA
LGIGAQGLGGLTTVLDIKIKDYPCHAAGKPVGMIPNCAATRHAHFQLDGSGPAHIQAPKLEDYPEVTWDSSKSKRVNLDT
ITQEEMNSWQPGDTLLLSGTMYTGRDAAHKRLVEMIDNGEELPVDLKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFT
RKVLEHTGLFGMIGKADRGPTAIEAIKDNQATYLMAVGGAAYLVSKAVREAEVVAFADLGMEAIYKFVVEDMPVSVAVDV
NGTSVHAIAPKIWQEKIGKIPVIDATAV

Sequences:

>Translated_508_residues
MTTIIKQEDLITSVKDALQFISYYHPQDFIQAMSRAYDREANQAAKDAIAQILINSRMCAEGHRPICQDTGIVNVFVDVG
LDVKFDLSMSLDDAINEGVRQGYLENTNVLRASVLADPAFGRKNTKDNTPAVIHYKLVPGNKVDITVAAKGGGSENKSKL
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMMLAKEALMEEINMDELLRRGPENKIEELRIEIFEKVNA
LGIGAQGLGGLTTVLDIKIKDYPCHAAGKPVGMIPNCAATRHAHFQLDGSGPAHIQAPKLEDYPEVTWDSSKSKRVNLDT
ITQEEMNSWQPGDTLLLSGTMYTGRDAAHKRLVEMIDNGEELPVDLKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFT
RKVLEHTGLFGMIGKADRGPTAIEAIKDNQATYLMAVGGAAYLVSKAVREAEVVAFADLGMEAIYKFVVEDMPVSVAVDV
NGTSVHAIAPKIWQEKIGKIPVIDATAV
>Mature_507_residues
TTIIKQEDLITSVKDALQFISYYHPQDFIQAMSRAYDREANQAAKDAIAQILINSRMCAEGHRPICQDTGIVNVFVDVGL
DVKFDLSMSLDDAINEGVRQGYLENTNVLRASVLADPAFGRKNTKDNTPAVIHYKLVPGNKVDITVAAKGGGSENKSKLA
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMMLAKEALMEEINMDELLRRGPENKIEELRIEIFEKVNAL
GIGAQGLGGLTTVLDIKIKDYPCHAAGKPVGMIPNCAATRHAHFQLDGSGPAHIQAPKLEDYPEVTWDSSKSKRVNLDTI
TQEEMNSWQPGDTLLLSGTMYTGRDAAHKRLVEMIDNGEELPVDLKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTR
KVLEHTGLFGMIGKADRGPTAIEAIKDNQATYLMAVGGAAYLVSKAVREAEVVAFADLGMEAIYKFVVEDMPVSVAVDVN
GTSVHAIAPKIWQEKIGKIPVIDATAV

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=468, Percent_Identity=33.3333333333333, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787897, Length=468, Percent_Identity=33.974358974359, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789443, Length=188, Percent_Identity=34.0425531914894, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789442, Length=272, Percent_Identity=28.3088235294118, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54966; Mature: 54835

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIIKQEDLITSVKDALQFISYYHPQDFIQAMSRAYDREANQAAKDAIAQILINSRMCA
CCCEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHC
EGHRPICQDTGIVNVFVDVGLDVKFDLSMSLDDAINEGVRQGYLENTNVLRASVLADPAF
CCCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEHHHCCCCC
GRKNTKDNTPAVIHYKLVPGNKVDITVAAKGGGSENKSKLAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMMLAKEALMEEINMDELLRRGPENKIEELRIEIFEKVNA
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDIKIKDYPCHAAGKPVGMIPNCAATRHAHFQLDGSGPAHIQAPKL
HCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCC
EDYPEVTWDSSKSKRVNLDTITQEEMNSWQPGDTLLLSGTMYTGRDAAHKRLVEMIDNGE
CCCCCCCCCCCCCCEECHHHCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHHHHCCCC
ELPVDLKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRKVLEHTGLFGMIGKADRGP
CCCEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCC
TAIEAIKDNQATYLMAVGGAAYLVSKAVREAEVVAFADLGMEAIYKFVVEDMPVSVAVDV
HHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHEEHHHHHCHHHHHHHHHHCCCEEEEEEC
NGTSVHAIAPKIWQEKIGKIPVIDATAV
CCCEEEEHHHHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
TTIIKQEDLITSVKDALQFISYYHPQDFIQAMSRAYDREANQAAKDAIAQILINSRMCA
CCEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHC
EGHRPICQDTGIVNVFVDVGLDVKFDLSMSLDDAINEGVRQGYLENTNVLRASVLADPAF
CCCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEHHHCCCCC
GRKNTKDNTPAVIHYKLVPGNKVDITVAAKGGGSENKSKLAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMMLAKEALMEEINMDELLRRGPENKIEELRIEIFEKVNA
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDIKIKDYPCHAAGKPVGMIPNCAATRHAHFQLDGSGPAHIQAPKL
HCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCC
EDYPEVTWDSSKSKRVNLDTITQEEMNSWQPGDTLLLSGTMYTGRDAAHKRLVEMIDNGE
CCCCCCCCCCCCCCEECHHHCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHHHHCCCC
ELPVDLKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRKVLEHTGLFGMIGKADRGP
CCCEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCC
TAIEAIKDNQATYLMAVGGAAYLVSKAVREAEVVAFADLGMEAIYKFVVEDMPVSVAVDV
HHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHEEHHHHHCHHHHHHHHHHCCCEEEEEEC
NGTSVHAIAPKIWQEKIGKIPVIDATAV
CCCEEEEHHHHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]