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The map label for this gene is topA [H]

Identifier: 50083732

GI number: 50083732

Start: 484929

End: 487574

Strand: Direct

Name: topA [H]

Synonym: ACIAD0493

Alternate gene names: 50083732

Gene position: 484929-487574 (Clockwise)

Preceding gene: 50083731

Following gene: 50083733

Centisome position: 13.48

GC content: 44.94

Gene sequence:

>2646_bases
ATGGCGAATGCCCCTCGTTCCACCACCAAAAAAAGTACGACAGCGTCTTCTGAGCCTGCTGGTAATAAACGTGCACTGGT
GATTGTGGAGTCGCCTGCGAAAGCGAAAACCATTAATAAATATCTGGGTTCACAATATATTGTTAAATCTTCTGTCGGCC
ATGTCCGTGATTTGCCAACAGGGGGTTCTGCCAAGTCCGGTGAAAAAAAGCCAGCAACGCGCGCTAAACTGACTGCCGAA
GAAAAAGAGCAGAAAGCACAAAATGCGTTGATTAATCGCATGGGTGTTGATCCTGAACATGACTGGAAAGCCCATTATGA
AGTTCTACCAGGTAAAGAACATGTCGTTGCAGAACTGAAAAAACTCGCTTCACAAGTTGACGAAGTTTATCTGGCAACGG
ATTTGGACCGTGAAGGAGAAGCCATTGCCTGGCATTTAAAAGAAGTCATTGGCGGTGATGACAGTCGTTATCAACGTGTT
GTATTTAATGAAATTACCAAAAATGCCATTCAGGAAGCATTTAAGCATCCAGCGCGTTTAGACACCAATAAAGTCAATGC
ACAGCAGGCACGCCGTTTTCTGGATCGTGTTGTCGGCTTTATGATTTCGCCATTACTTTGGGAAAAAATTGCACGGGGTT
TATCTGCGGGGCGTGTTCAATCGGTTGCAGTGAAGCTGGTGGTTGAGCGTGAACGGGATATTCGTGCATTCATTCCAGAA
GAATACTGGCAGATTTTTGCAGATACCCTGTCTAAAAAAGATCAGATTCGTTTGGAAGCCGTCAAACAGGCAGGTAAGAC
ACTTAAACTCAGAAACAAAGCGGACACCGATGCGGTTTTAAATCTGCTTAAGGATGCCGAGTATAAGGTTGCACAACGCG
AGGATAAACCGACCAAGGTGAATCCAAGTGCGCCCTATATCACCTCGACTTTACAGCAGGCTGCCAGTACTCGACTTGGT
TTTTCAGTGAAAAAAACCATGATGCTGGCACAAAGACTATATGAAGGTGGTTTCATCACCTACATGCGTACCGACTCGAC
CTTCTTGAGTGATGACGCAGTCAATATGGTGCGTGATCATATTCAGCAACAGTTTGGTGACAAATATGTCCCTGCAAAAC
CAAATCGTTATGGCAATAAAGCAGGTGCACAAGAAGCGCATGAAGCGATTCGTCCTTCTAATGTTGCTCTAACAGGTGAT
CAGCTTGCAGGTATGGAGCGTGATGCACAGCGTCTTTACGATTTGATCTGGCGTCAATTTGTGGCCTGTCAAATGACACC
TGCCGAATATTTATCTTCTACTTTGATTGTTGATGCCAACAATGTTGAGTTACGTGCCAAAGGCCGTACCTTGGTATTTG
ATGGATTTACCAGAGTTCGTGGTGCCAACAAATCTGACGATGATGTGTTATTACCAGCAGTGAAAGTGGGTGAAACGCTC
AAGCTTGAAAAACTTGACCCAAGTCAGCATTTTACCAAACCACCTGCACGTTTTACCGAAGCATCTTTGGTCAAAGAGCT
GGAAAAACGTGGAATTGGCCGTCCATCGACATACGCTGCGATTATTTCTACGATTCAAGATCGTGGTTACGTCAAACTTG
AAAATCGTCGTTTATTTGCCGAAAAAATGGGCGAGATCGTCACCGATCGTCTCGATGAAAGCTTTAATAACCTGATGAAC
TATGCATTTACTGCTGATCTTGAAGGCCAGTTGGATAAAGTAGCGACAGGTGAGCGTAACTGGAAAGAGTTACTGGATAG
TTTCTATGGTGATTTTAAAAACCGTTTAAATATGGCTGCCAGTACCGAAGGCATGCGTCGTAACCAGCCTGTTGAAGTAC
CAGATGTGGCATGTCAGCTCTGTAGCCGTCCAATGCAGATTCGTACTGGAACCACAGGCGTATTTTTAGGCTGTTCGGGG
TATAACCTACCACCAAAAGAACGTTGTAAAGGAACGCTTAATTTAACACCAGTCGAATCACTGGCTGCTTTCTCTGATGA
TGATTCTGCCGAAACTGCAGATTTGATGGCGAAGCATCGCTGTGCAAAATGCGGTACTGCCATGGATAGTTATGTGATTG
ATGGTGGTCGTAAACTTCATGTGTGTGGTAACAATCCAGACTGCGACGGTTATGAAGTTGAAGAGGGCGAATTCAAAATT
AAGGGTTATGATGGGCCCACCATTCCATGTGATAAGTGTGATGGTGAAATGCAGTTAAAGACAGGACGTTTTGGTCCTTA
TTTTGCCTGCACCAGTTGCGATAATACCCGTAAGGTATTGAAAAGTGGTCAACCCGCACCGCCACGTGTAGATCCAATCA
AGATGGAGCATTTGCGTTCGGTCAAGCATGATGACTATTTTGTATTGCGTGATGGTGCAGCAGGTCTTTTTCTGGCAGCC
AGCAAGTTTCCAAAAATCCGTGAAACGCGAGCACCCAAAGTGGCAGAATTACGGACAGTTGCTGAACAGCTTGATCCAAA
ATATCAATACTTGCTACAAGCACCTGATGCAGATCCAGAGGGTAATCCAGCACTGATCAAATTTAGCCGTAAAAATCAGG
CGCAATATGTCGGTTCTGAAACGCCTGAAGGCAAGCAGACCAAATGGAGTCTGGTATATCAGGATGGAAAATGGACCGAA
GCTTAG

Upstream 100 bases:

>100_bases
AGACCTTAAAGCCACACAACTTGAAGAGATGAAATCGTATTTACACAGTCTGGCGACTGTCTAATTTTTAATTTATCCAT
TATAGGAATCACTGTTGATT

Downstream 100 bases:

>100_bases
GTTTTTGATTCTATTAAAAATGCCGACACATTGTGTCGGCATTTTTTATGAAGCTAATTTTCTATTAGATATAAGATATA
GATGTTTATATTTCGATAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 881; Mature: 880

Protein sequence:

>881_residues
MANAPRSTTKKSTTASSEPAGNKRALVIVESPAKAKTINKYLGSQYIVKSSVGHVRDLPTGGSAKSGEKKPATRAKLTAE
EKEQKAQNALINRMGVDPEHDWKAHYEVLPGKEHVVAELKKLASQVDEVYLATDLDREGEAIAWHLKEVIGGDDSRYQRV
VFNEITKNAIQEAFKHPARLDTNKVNAQQARRFLDRVVGFMISPLLWEKIARGLSAGRVQSVAVKLVVERERDIRAFIPE
EYWQIFADTLSKKDQIRLEAVKQAGKTLKLRNKADTDAVLNLLKDAEYKVAQREDKPTKVNPSAPYITSTLQQAASTRLG
FSVKKTMMLAQRLYEGGFITYMRTDSTFLSDDAVNMVRDHIQQQFGDKYVPAKPNRYGNKAGAQEAHEAIRPSNVALTGD
QLAGMERDAQRLYDLIWRQFVACQMTPAEYLSSTLIVDANNVELRAKGRTLVFDGFTRVRGANKSDDDVLLPAVKVGETL
KLEKLDPSQHFTKPPARFTEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKLENRRLFAEKMGEIVTDRLDESFNNLMN
YAFTADLEGQLDKVATGERNWKELLDSFYGDFKNRLNMAASTEGMRRNQPVEVPDVACQLCSRPMQIRTGTTGVFLGCSG
YNLPPKERCKGTLNLTPVESLAAFSDDDSAETADLMAKHRCAKCGTAMDSYVIDGGRKLHVCGNNPDCDGYEVEEGEFKI
KGYDGPTIPCDKCDGEMQLKTGRFGPYFACTSCDNTRKVLKSGQPAPPRVDPIKMEHLRSVKHDDYFVLRDGAAGLFLAA
SKFPKIRETRAPKVAELRTVAEQLDPKYQYLLQAPDADPEGNPALIKFSRKNQAQYVGSETPEGKQTKWSLVYQDGKWTE
A

Sequences:

>Translated_881_residues
MANAPRSTTKKSTTASSEPAGNKRALVIVESPAKAKTINKYLGSQYIVKSSVGHVRDLPTGGSAKSGEKKPATRAKLTAE
EKEQKAQNALINRMGVDPEHDWKAHYEVLPGKEHVVAELKKLASQVDEVYLATDLDREGEAIAWHLKEVIGGDDSRYQRV
VFNEITKNAIQEAFKHPARLDTNKVNAQQARRFLDRVVGFMISPLLWEKIARGLSAGRVQSVAVKLVVERERDIRAFIPE
EYWQIFADTLSKKDQIRLEAVKQAGKTLKLRNKADTDAVLNLLKDAEYKVAQREDKPTKVNPSAPYITSTLQQAASTRLG
FSVKKTMMLAQRLYEGGFITYMRTDSTFLSDDAVNMVRDHIQQQFGDKYVPAKPNRYGNKAGAQEAHEAIRPSNVALTGD
QLAGMERDAQRLYDLIWRQFVACQMTPAEYLSSTLIVDANNVELRAKGRTLVFDGFTRVRGANKSDDDVLLPAVKVGETL
KLEKLDPSQHFTKPPARFTEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKLENRRLFAEKMGEIVTDRLDESFNNLMN
YAFTADLEGQLDKVATGERNWKELLDSFYGDFKNRLNMAASTEGMRRNQPVEVPDVACQLCSRPMQIRTGTTGVFLGCSG
YNLPPKERCKGTLNLTPVESLAAFSDDDSAETADLMAKHRCAKCGTAMDSYVIDGGRKLHVCGNNPDCDGYEVEEGEFKI
KGYDGPTIPCDKCDGEMQLKTGRFGPYFACTSCDNTRKVLKSGQPAPPRVDPIKMEHLRSVKHDDYFVLRDGAAGLFLAA
SKFPKIRETRAPKVAELRTVAEQLDPKYQYLLQAPDADPEGNPALIKFSRKNQAQYVGSETPEGKQTKWSLVYQDGKWTE
A
>Mature_880_residues
ANAPRSTTKKSTTASSEPAGNKRALVIVESPAKAKTINKYLGSQYIVKSSVGHVRDLPTGGSAKSGEKKPATRAKLTAEE
KEQKAQNALINRMGVDPEHDWKAHYEVLPGKEHVVAELKKLASQVDEVYLATDLDREGEAIAWHLKEVIGGDDSRYQRVV
FNEITKNAIQEAFKHPARLDTNKVNAQQARRFLDRVVGFMISPLLWEKIARGLSAGRVQSVAVKLVVERERDIRAFIPEE
YWQIFADTLSKKDQIRLEAVKQAGKTLKLRNKADTDAVLNLLKDAEYKVAQREDKPTKVNPSAPYITSTLQQAASTRLGF
SVKKTMMLAQRLYEGGFITYMRTDSTFLSDDAVNMVRDHIQQQFGDKYVPAKPNRYGNKAGAQEAHEAIRPSNVALTGDQ
LAGMERDAQRLYDLIWRQFVACQMTPAEYLSSTLIVDANNVELRAKGRTLVFDGFTRVRGANKSDDDVLLPAVKVGETLK
LEKLDPSQHFTKPPARFTEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKLENRRLFAEKMGEIVTDRLDESFNNLMNY
AFTADLEGQLDKVATGERNWKELLDSFYGDFKNRLNMAASTEGMRRNQPVEVPDVACQLCSRPMQIRTGTTGVFLGCSGY
NLPPKERCKGTLNLTPVESLAAFSDDDSAETADLMAKHRCAKCGTAMDSYVIDGGRKLHVCGNNPDCDGYEVEEGEFKIK
GYDGPTIPCDKCDGEMQLKTGRFGPYFACTSCDNTRKVLKSGQPAPPRVDPIKMEHLRSVKHDDYFVLRDGAAGLFLAAS
KFPKIRETRAPKVAELRTVAEQLDPKYQYLLQAPDADPEGNPALIKFSRKNQAQYVGSETPEGKQTKWSLVYQDGKWTEA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=569, Percent_Identity=27.0650263620387, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI4507635, Length=555, Percent_Identity=26.3063063063063, Blast_Score=137, Evalue=4e-32,
Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=63.5838150289017, Blast_Score=1128, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=548, Percent_Identity=22.8102189781022, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32563869, Length=543, Percent_Identity=25.414364640884, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=515, Percent_Identity=26.4077669902913, Blast_Score=128, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=479, Percent_Identity=26.5135699373695, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585251, Length=486, Percent_Identity=27.9835390946502, Blast_Score=129, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640096, Length=511, Percent_Identity=24.4618395303327, Blast_Score=117, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98252; Mature: 98120

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAPRSTTKKSTTASSEPAGNKRALVIVESPAKAKTINKYLGSQYIVKSSVGHVRDLPT
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHCCCHHHCCCC
GGSAKSGEKKPATRAKLTAEEKEQKAQNALINRMGVDPEHDWKAHYEVLPGKEHVVAELK
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCHHHHHHHHH
KLASQVDEVYLATDLDREGEAIAWHLKEVIGGDDSRYQRVVFNEITKNAIQEAFKHPARL
HHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DTNKVNAQQARRFLDRVVGFMISPLLWEKIARGLSAGRVQSVAVKLVVERERDIRAFIPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCH
EYWQIFADTLSKKDQIRLEAVKQAGKTLKLRNKADTDAVLNLLKDAEYKVAQREDKPTKV
HHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCHHHHHHCCCCCCCC
NPSAPYITSTLQQAASTRLGFSVKKTMMLAQRLYEGGFITYMRTDSTFLSDDAVNMVRDH
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
IQQQFGDKYVPAKPNRYGNKAGAQEAHEAIRPSNVALTGDQLAGMERDAQRLYDLIWRQF
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECHHHHCCHHHHHHHHHHHHHHH
VACQMTPAEYLSSTLIVDANNVELRAKGRTLVFDGFTRVRGANKSDDDVLLPAVKVGETL
HHHCCCHHHHCCCEEEEECCCEEEEECCCEEEEECHHHHCCCCCCCCCEEECHHHCCCEE
KLEKLDPSQHFTKPPARFTEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKLENRRLFA
EEECCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHH
EKMGEIVTDRLDESFNNLMNYAFTADLEGQLDKVATGERNWKELLDSFYGDFKNRLNMAA
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
STEGMRRNQPVEVPDVACQLCSRPMQIRTGTTGVFLGCSGYNLPPKERCKGTLNLTPVES
CCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCHHHHCCCCCCCCCHHH
LAAFSDDDSAETADLMAKHRCAKCGTAMDSYVIDGGRKLHVCGNNPDCDGYEVEEGEFKI
HHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHEECCCCEEEEECCCCCCCCEEECCCEEEE
KGYDGPTIPCDKCDGEMQLKTGRFGPYFACTSCDNTRKVLKSGQPAPPRVDPIKMEHLRS
ECCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHH
VKHDDYFVLRDGAAGLFLAASKFPKIRETRAPKVAELRTVAEQLDPKYQYLLQAPDADPE
CCCCCEEEEECCCCCEEEEHHCCCCHHHHCCCHHHHHHHHHHHHCCCEEHHEECCCCCCC
GNPALIKFSRKNQAQYVGSETPEGKQTKWSLVYQDGKWTEA
CCCEEEEEECCCCHHHCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
ANAPRSTTKKSTTASSEPAGNKRALVIVESPAKAKTINKYLGSQYIVKSSVGHVRDLPT
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHCCCHHHCCCC
GGSAKSGEKKPATRAKLTAEEKEQKAQNALINRMGVDPEHDWKAHYEVLPGKEHVVAELK
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCHHHHHHHHH
KLASQVDEVYLATDLDREGEAIAWHLKEVIGGDDSRYQRVVFNEITKNAIQEAFKHPARL
HHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DTNKVNAQQARRFLDRVVGFMISPLLWEKIARGLSAGRVQSVAVKLVVERERDIRAFIPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCH
EYWQIFADTLSKKDQIRLEAVKQAGKTLKLRNKADTDAVLNLLKDAEYKVAQREDKPTKV
HHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCHHHHHHCCCCCCCC
NPSAPYITSTLQQAASTRLGFSVKKTMMLAQRLYEGGFITYMRTDSTFLSDDAVNMVRDH
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
IQQQFGDKYVPAKPNRYGNKAGAQEAHEAIRPSNVALTGDQLAGMERDAQRLYDLIWRQF
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECHHHHCCHHHHHHHHHHHHHHH
VACQMTPAEYLSSTLIVDANNVELRAKGRTLVFDGFTRVRGANKSDDDVLLPAVKVGETL
HHHCCCHHHHCCCEEEEECCCEEEEECCCEEEEECHHHHCCCCCCCCCEEECHHHCCCEE
KLEKLDPSQHFTKPPARFTEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKLENRRLFA
EEECCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHH
EKMGEIVTDRLDESFNNLMNYAFTADLEGQLDKVATGERNWKELLDSFYGDFKNRLNMAA
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
STEGMRRNQPVEVPDVACQLCSRPMQIRTGTTGVFLGCSGYNLPPKERCKGTLNLTPVES
CCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCHHHHCCCCCCCCCHHH
LAAFSDDDSAETADLMAKHRCAKCGTAMDSYVIDGGRKLHVCGNNPDCDGYEVEEGEFKI
HHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHEECCCCEEEEECCCCCCCCEEECCCEEEE
KGYDGPTIPCDKCDGEMQLKTGRFGPYFACTSCDNTRKVLKSGQPAPPRVDPIKMEHLRS
ECCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHH
VKHDDYFVLRDGAAGLFLAASKFPKIRETRAPKVAELRTVAEQLDPKYQYLLQAPDADPE
CCCCCEEEEECCCCCEEEEHHCCCCHHHHCCCHHHHHHHHHHHHCCCEEHHEECCCCCCC
GNPALIKFSRKNQAQYVGSETPEGKQTKWSLVYQDGKWTEA
CCCEEEEEECCCCHHHCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]