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The map label for this gene is leuB

Identifier: 50083714

GI number: 50083714

Start: 464538

End: 465617

Strand: Direct

Name: leuB

Synonym: ACIAD0469

Alternate gene names: 50083714

Gene position: 464538-465617 (Clockwise)

Preceding gene: 50083713

Following gene: 50083715

Centisome position: 12.91

GC content: 44.26

Gene sequence:

>1080_bases
ATGTCGAAGCAAATTTTAATTTTAGCTGGTGATGGTATTGGTCCTGAAATTGTGGGGGCTGCCGAGCAAGTATTAACTAA
GGTCAATGAAAAGTTTGAACTTGGATTGACTTGGGAACATGGCTTACTCGGTGGTTCTGCCATCGATGCCCATGGTGAAC
CCTATCCTGCCGTTACAAGTGAACAAGCAAAAAAAGCAGATGCGATTTTACTTGGCGCCGTCGGTGGACCTAAATGGGAT
ACGATTGAGCGTTCGATTCGTCCTGAGCGTGGATTGCTTAAAATTCGTAGTGAACTGAATCTATTTGCTAACCTTCGTCC
AGCGCTTTTATATCCTCAGCTTGCAGATGCATCTAGTTTAAAACCAGAAATCGTTGCGGGTCTGGATATTTTGATTGTAC
GTGAATTGACGGGTGGTATTTATTTCGGTCAGCCACGCGGAATTCGCGAACTCGAAAATGGTGAGAAACAAGGCTATAAC
ACTGATGTTTACAGTGAGTCTGAAATTAAACGCATTGCAAAAGTGGCATTTGAGCTCGCGGCATTGCGCGGTAGTAAAGT
GTGCTCGGTTGATAAGGCTAATGTACTTGAAGTAACTGAATTGTGGAAGCAAACAGTGACTGAGTTACAGCAAGCTCAAT
ATCCAAATATTCAATTGTCACACATGTATGTTGATAATGCGGCGATGCAATTGGTACGTGCACCTAAACAGTTTGACGTC
ATTGTTACAGGCAACTTGTTTGGTGATATTTTATCTGATGAAGCTGCAATGCTGACAGGCTCAATTGGCATGTTGCCATC
TGCATCGTTAGATGAAAACGGTAAAGGCATGTATGAACCTTGTCATGGTTCTGCGCCAGATATCGCAGGTCAGAACGTTG
CTAATCCATTGGCTACCATTTTATCGGTGGCCATGATGCTGCGTTATACTTTCCGTGAAGAAGCTGCTGCAAAAGCGATT
GAAGATGCAGTCGGTCAAGTGCTTGATCAAGGCTTACGTACAGCAGATATCATGTCTGAAGGCATGCAAAGAGTGGGTAC
CGTTGAGATGGGGCAGGCGGTTGTTGCTGCTTTGGCTTAA

Upstream 100 bases:

>100_bases
TCCTTTTGATGCAGTGACAGGATTGGGTGAGTTTATTGAACAACGAATTTAATGATCAATCAGCACGTGTGATGCTGAAT
TAGTAAAAATGGAAACGTTC

Downstream 100 bases:

>100_bases
ATAATATTGCCCGAACACCGATCTTAAAAGGAGATGCCTTGAACAAGCATTTCCTTTTTTAATATTATAAAAATAAATTA
TTGTGATAAGTTAGAGACAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSKQILILAGDGIGPEIVGAAEQVLTKVNEKFELGLTWEHGLLGGSAIDAHGEPYPAVTSEQAKKADAILLGAVGGPKWD
TIERSIRPERGLLKIRSELNLFANLRPALLYPQLADASSLKPEIVAGLDILIVRELTGGIYFGQPRGIRELENGEKQGYN
TDVYSESEIKRIAKVAFELAALRGSKVCSVDKANVLEVTELWKQTVTELQQAQYPNIQLSHMYVDNAAMQLVRAPKQFDV
IVTGNLFGDILSDEAAMLTGSIGMLPSASLDENGKGMYEPCHGSAPDIAGQNVANPLATILSVAMMLRYTFREEAAAKAI
EDAVGQVLDQGLRTADIMSEGMQRVGTVEMGQAVVAALA

Sequences:

>Translated_359_residues
MSKQILILAGDGIGPEIVGAAEQVLTKVNEKFELGLTWEHGLLGGSAIDAHGEPYPAVTSEQAKKADAILLGAVGGPKWD
TIERSIRPERGLLKIRSELNLFANLRPALLYPQLADASSLKPEIVAGLDILIVRELTGGIYFGQPRGIRELENGEKQGYN
TDVYSESEIKRIAKVAFELAALRGSKVCSVDKANVLEVTELWKQTVTELQQAQYPNIQLSHMYVDNAAMQLVRAPKQFDV
IVTGNLFGDILSDEAAMLTGSIGMLPSASLDENGKGMYEPCHGSAPDIAGQNVANPLATILSVAMMLRYTFREEAAAKAI
EDAVGQVLDQGLRTADIMSEGMQRVGTVEMGQAVVAALA
>Mature_358_residues
SKQILILAGDGIGPEIVGAAEQVLTKVNEKFELGLTWEHGLLGGSAIDAHGEPYPAVTSEQAKKADAILLGAVGGPKWDT
IERSIRPERGLLKIRSELNLFANLRPALLYPQLADASSLKPEIVAGLDILIVRELTGGIYFGQPRGIRELENGEKQGYNT
DVYSESEIKRIAKVAFELAALRGSKVCSVDKANVLEVTELWKQTVTELQQAQYPNIQLSHMYVDNAAMQLVRAPKQFDVI
VTGNLFGDILSDEAAMLTGSIGMLPSASLDENGKGMYEPCHGSAPDIAGQNVANPLATILSVAMMLRYTFREEAAAKAIE
DAVGQVLDQGLRTADIMSEGMQRVGTVEMGQAVVAALA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=354, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI28178816, Length=361, Percent_Identity=29.0858725761773, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178821, Length=309, Percent_Identity=30.7443365695793, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4758582, Length=252, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178838, Length=240, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=163, Percent_Identity=36.1963190184049, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=52.6170798898072, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1788101, Length=345, Percent_Identity=35.3623188405797, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1787381, Length=397, Percent_Identity=26.9521410579345, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=32.183908045977, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=349, Percent_Identity=30.945558739255, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17550882, Length=340, Percent_Identity=30.8823529411765, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=338, Percent_Identity=28.9940828402367, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=45.8563535911602, Blast_Score=301, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=35.7142857142857, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=30.952380952381, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324709, Length=343, Percent_Identity=32.0699708454811, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=33.1378299120235, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=33.1378299120235, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=30.7017543859649, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078633, Length=338, Percent_Identity=27.5147928994083, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650122, Length=338, Percent_Identity=27.5147928994083, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078637, Length=338, Percent_Identity=27.5147928994083, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078635, Length=338, Percent_Identity=27.5147928994083, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078639, Length=336, Percent_Identity=27.6785714285714, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=28.9085545722714, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=28.9085545722714, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_ACIAD (Q6FEV6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045224.1
- ProteinModelPortal:   Q6FEV6
- SMR:   Q6FEV6
- STRING:   Q6FEV6
- GeneID:   2880113
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0469
- NMPDR:   fig|62977.3.peg.1122
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- PhylomeDB:   Q6FEV6
- ProtClustDB:   PRK00772
- BioCyc:   ASP62977:ACIAD0469-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38545; Mature: 38414

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 134-134 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQILILAGDGIGPEIVGAAEQVLTKVNEKFELGLTWEHGLLGGSAIDAHGEPYPAVTS
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCCCCCCCCC
EQAKKADAILLGAVGGPKWDTIERSIRPERGLLKIRSELNLFANLRPALLYPQLADASSL
HHHHCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
KPEIVAGLDILIVRELTGGIYFGQPRGIRELENGEKQGYNTDVYSESEIKRIAKVAFELA
CCHHHHCHHHEEEEHHHCCEEECCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALRGSKVCSVDKANVLEVTELWKQTVTELQQAQYPNIQLSHMYVDNAAMQLVRAPKQFDV
HHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCEEE
IVTGNLFGDILSDEAAMLTGSIGMLPSASLDENGKGMYEPCHGSAPDIAGQNVANPLATI
EEECHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSVAMMLRYTFREEAAAKAIEDAVGQVLDQGLRTADIMSEGMQRVGTVEMGQAVVAALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKQILILAGDGIGPEIVGAAEQVLTKVNEKFELGLTWEHGLLGGSAIDAHGEPYPAVTS
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCCCCCCCCC
EQAKKADAILLGAVGGPKWDTIERSIRPERGLLKIRSELNLFANLRPALLYPQLADASSL
HHHHCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
KPEIVAGLDILIVRELTGGIYFGQPRGIRELENGEKQGYNTDVYSESEIKRIAKVAFELA
CCHHHHCHHHEEEEHHHCCEEECCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALRGSKVCSVDKANVLEVTELWKQTVTELQQAQYPNIQLSHMYVDNAAMQLVRAPKQFDV
HHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCEEE
IVTGNLFGDILSDEAAMLTGSIGMLPSASLDENGKGMYEPCHGSAPDIAGQNVANPLATI
EEECHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSVAMMLRYTFREEAAAKAIEDAVGQVLDQGLRTADIMSEGMQRVGTVEMGQAVVAALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA