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The map label for this gene is leuC

Identifier: 50083710

GI number: 50083710

Start: 461051

End: 462484

Strand: Direct

Name: leuC

Synonym: ACIAD0463

Alternate gene names: 50083710

Gene position: 461051-462484 (Clockwise)

Preceding gene: 50083706

Following gene: 50083711

Centisome position: 12.81

GC content: 45.82

Gene sequence:

>1434_bases
ATGGCAGGCACGACCCAAGGCGCTAAAACCTTGTACGACAAATTATGGGATGACCATTTGGTTTCACAACGAGACGATGG
TTCATGCTTGATTTATATTGACCGTCATTTATTGCATGAAGTGACCAGTCCACAAGCTTTTGAAGGTTTACAGCTTGCAG
GTCGTCAGCCTTGGCGTCTAAATGCCAATATCGCGACACCAGATCACAATGTTCCAACCTCTAAAAAAGAGCGTGAGCAA
GGAATTGCGGGTATTGAAGATGATACCTCACGTATTCAGGTTCAGACGCTAGATGATAACTGTAAGACATTTAATATCGT
TGAGTTTGGTATCAATGATATTCGTCAAGGCATTGTGCATGTTGTCGGGCCAGAGCAGGGTTTAACCTTACCAGGCATGA
CCGTTGTCTGTGGCGATTCGCATACAGCGACGCATGGTGCTTTTGGTTGTTTGGCGCACGGTATTGGTACATCTGAAGTG
GAACATGTGTTAGCAACACAATGTTTGATTCAGAAAAAATCTAAAAACATGTTGATTCGTGTAGATGGCCAGTTAGGCAA
AGGTGTGACACCCAAAGACGTGGTATTGGCGATCATTGGTAAAATTGGCACTGCAGGCGGAACAGGCTATGCCATCGAAT
TTGGTGGACAGGTCTTCCGTGACATGTCGATTGAAGGCCGCATGACTGTATGTAACATGGCGATTGAAGCAGGCGCACGT
GTGGGTATGGTGGCTGTAGATGATAAAACCATTGAATATGTACAATCTCGTCATTACGCACCAAAGGGCGAACAATGGGA
TCAGGCAGTAGCCTACTGGAATACCTTGCATTCAGATGATGATGCGGTATTTGATGAAGTTGTTGTATTAAATGGTGCAG
AGATTGAACCACAGGTATCTTGGGGAACATCTCCTGAAATGGTGATTCCAGTATCTCAAGCTGTTCCAACTTTAGAACAG
GCAAAAGACGACGTACAACGCAATGACTGGACACGTGCATACCAATATATGGGCCTAACAGCAGGTCAGGCGTTGGCTGA
TATTCAGCTAGATCGTGTATTTATTGGTTCGTGCACCAACTCACGTATCGAAGATATTCGTGCAGCGGCAGAGGTCGTGA
AAGGTCGTAAAGTTGCTTCGAGCATTAAGCAAGCGATGATTGTTCCTGGTTCTGGTTTGGTCAAGCAGCAAGCAGAGCAA
GAAGGTTTGGATCAGGTGTTCCTTGAAGCTGGTTTTGAATGGCGTGAACCAGGTTGTTCGATGTGTTTGGCGATGAACTC
TGACAAATTACAGCCAGGTGAACACTGTGCTTCGACCTCAAATCGTAATTTTGAAGGACGTCAGGGCAATGGCGGACGCA
CCCATTTGGTAAGTCCAGCAATGGCAGCAGCAGCAGCAATTGCAGGTCACTTCGTCGATGTTAGAAGTTTCTAA

Upstream 100 bases:

>100_bases
TTTTTTAATTAATAAAAATGATAAATTAGATTTATGTGAAACTGTGCTTATAATAATAAAAATACAGAATACGATACCCA
GTCCAATGGAGCATGACGAC

Downstream 100 bases:

>100_bases
TGAGCATGACTTTGTATCTGGGAACAAAAAATTATTCTTCATGGTCTTTGAGACCTTGGCTTGCCGCAAGAAAAGCAGCT
ATTAAATTTGATGAAATAAT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAGTTQGAKTLYDKLWDDHLVSQRDDGSCLIYIDRHLLHEVTSPQAFEGLQLAGRQPWRLNANIATPDHNVPTSKKEREQ
GIAGIEDDTSRIQVQTLDDNCKTFNIVEFGINDIRQGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEV
EHVLATQCLIQKKSKNMLIRVDGQLGKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGAR
VGMVAVDDKTIEYVQSRHYAPKGEQWDQAVAYWNTLHSDDDAVFDEVVVLNGAEIEPQVSWGTSPEMVIPVSQAVPTLEQ
AKDDVQRNDWTRAYQYMGLTAGQALADIQLDRVFIGSCTNSRIEDIRAAAEVVKGRKVASSIKQAMIVPGSGLVKQQAEQ
EGLDQVFLEAGFEWREPGCSMCLAMNSDKLQPGEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAIAGHFVDVRSF

Sequences:

>Translated_477_residues
MAGTTQGAKTLYDKLWDDHLVSQRDDGSCLIYIDRHLLHEVTSPQAFEGLQLAGRQPWRLNANIATPDHNVPTSKKEREQ
GIAGIEDDTSRIQVQTLDDNCKTFNIVEFGINDIRQGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEV
EHVLATQCLIQKKSKNMLIRVDGQLGKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGAR
VGMVAVDDKTIEYVQSRHYAPKGEQWDQAVAYWNTLHSDDDAVFDEVVVLNGAEIEPQVSWGTSPEMVIPVSQAVPTLEQ
AKDDVQRNDWTRAYQYMGLTAGQALADIQLDRVFIGSCTNSRIEDIRAAAEVVKGRKVASSIKQAMIVPGSGLVKQQAEQ
EGLDQVFLEAGFEWREPGCSMCLAMNSDKLQPGEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAIAGHFVDVRSF
>Mature_476_residues
AGTTQGAKTLYDKLWDDHLVSQRDDGSCLIYIDRHLLHEVTSPQAFEGLQLAGRQPWRLNANIATPDHNVPTSKKEREQG
IAGIEDDTSRIQVQTLDDNCKTFNIVEFGINDIRQGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVE
HVLATQCLIQKKSKNMLIRVDGQLGKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGARV
GMVAVDDKTIEYVQSRHYAPKGEQWDQAVAYWNTLHSDDDAVFDEVVVLNGAEIEPQVSWGTSPEMVIPVSQAVPTLEQA
KDDVQRNDWTRAYQYMGLTAGQALADIQLDRVFIGSCTNSRIEDIRAAAEVVKGRKVASSIKQAMIVPGSGLVKQQAEQE
GLDQVFLEAGFEWREPGCSMCLAMNSDKLQPGEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAIAGHFVDVRSF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=361, Percent_Identity=27.1468144044321, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI41352693, Length=458, Percent_Identity=25.3275109170306, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8659555, Length=385, Percent_Identity=25.7142857142857, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=61.063829787234, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=25.4847645429363, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081781, Length=353, Percent_Identity=22.6628895184136, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=391, Percent_Identity=26.5984654731458, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=389, Percent_Identity=26.4781491002571, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=303, Percent_Identity=26.4026402640264, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17568399, Length=504, Percent_Identity=24.8015873015873, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=474, Percent_Identity=59.0717299578059, Blast_Score=580, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=29.5580110497238, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=363, Percent_Identity=28.6501377410468, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=352, Percent_Identity=27.5568181818182, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI161076999, Length=363, Percent_Identity=26.7217630853994, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI281365315, Length=363, Percent_Identity=26.4462809917355, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI17864292, Length=363, Percent_Identity=26.4462809917355, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI28571643, Length=383, Percent_Identity=26.1096605744125, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645686, Length=377, Percent_Identity=28.1167108753316, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137564, Length=384, Percent_Identity=27.8645833333333, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_ACIAD (Q6FEW0)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045220.1
- ProteinModelPortal:   Q6FEW0
- STRING:   Q6FEW0
- GeneID:   2880114
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0463
- NMPDR:   fig|62977.3.peg.1118
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q6FEW0
- ProtClustDB:   PRK05478
- BioCyc:   ASP62977:ACIAD0463-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51660; Mature: 51529

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTTQGAKTLYDKLWDDHLVSQRDDGSCLIYIDRHLLHEVTSPQAFEGLQLAGRQPWRL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCCHHCCHHHCCCCCEEE
NANIATPDHNVPTSKKEREQGIAGIEDDTSRIQVQTLDDNCKTFNIVEFGINDIRQGIVH
ECEECCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEECCHHHHHHCCEE
VVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVEHVLATQCLIQKKSKNMLIR
EECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
VDGQLGKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGAR
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCCCCCEEHHHHHCCCE
VGMVAVDDKTIEYVQSRHYAPKGEQWDQAVAYWNTLHSDDDAVFDEVVVLNGAEIEPQVS
EEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCC
WGTSPEMVIPVSQAVPTLEQAKDDVQRNDWTRAYQYMGLTAGQALADIQLDRVFIGSCTN
CCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECEEEEECCCC
SRIEDIRAAAEVVKGRKVASSIKQAMIVPGSGLVKQQAEQEGLDQVFLEAGFEWREPGCS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
MCLAMNSDKLQPGEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAIAGHFVDVRSF
EEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure 
AGTTQGAKTLYDKLWDDHLVSQRDDGSCLIYIDRHLLHEVTSPQAFEGLQLAGRQPWRL
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCCHHCCHHHCCCCCEEE
NANIATPDHNVPTSKKEREQGIAGIEDDTSRIQVQTLDDNCKTFNIVEFGINDIRQGIVH
ECEECCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEECCHHHHHHCCEE
VVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVEHVLATQCLIQKKSKNMLIR
EECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
VDGQLGKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGAR
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCCCCCEEHHHHHCCCE
VGMVAVDDKTIEYVQSRHYAPKGEQWDQAVAYWNTLHSDDDAVFDEVVVLNGAEIEPQVS
EEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCC
WGTSPEMVIPVSQAVPTLEQAKDDVQRNDWTRAYQYMGLTAGQALADIQLDRVFIGSCTN
CCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECEEEEECCCC
SRIEDIRAAAEVVKGRKVASSIKQAMIVPGSGLVKQQAEQEGLDQVFLEAGFEWREPGCS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
MCLAMNSDKLQPGEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAIAGHFVDVRSF
EEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA