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The map label for this gene is caiC [H]

Identifier: 50083689

GI number: 50083689

Start: 433978

End: 435825

Strand: Reverse

Name: caiC [H]

Synonym: ACIAD0437

Alternate gene names: 50083689

Gene position: 435825-433978 (Counterclockwise)

Preceding gene: 50083690

Following gene: 50083688

Centisome position: 12.11

GC content: 42.05

Gene sequence:

>1848_bases
ATGCATATGAGCCAGACCACACAGACCGACATCATTACGCTTGGCGCTGTTGCCGCAAAAATTCCTCAACTGATTCCAAA
GGTGCCTCATCTCCTCAATGGTCTCAAGCAAGCCTATTTAAGAACCCCAAGCACCCCTGCTGGTTTAGGTATCGCTTTTG
AAAAAGCAGTCAAACGTAATCCAGATGGTATGGCTTTACTATTTGAAGATGAACGCTATAGCTATCAGGAACTGAATGAA
TGGGCAAACCAGATCGCACACTTTTATTTATCATTAGGTGCGCGTAAAGGTGATGTCATTGCGGTGATGGTAGAAAACCG
TTCTGAACTGTTAGCAACCATCGTGGCGCTTGCCAAAATTGGGGTGACTGCTGCATTGGTCAATACTTCACAAACAGGCA
AAGTATTAACCCACAGTATTAATCTGGTTCAACCTATTGCACTGATTGTTGGTGAAGAAGTCCGTAAATGTGTCGATGAA
ATTAAACAAGATCTGAATCTTGCGCAGGATCGCTTTCATTGGTTTGCCAATCAGGCCACTCGTCAAGATGCGGGCTCTGC
ACCACGTGACTACATCAATCTGGCAGAAAAAATCGATCATTTCCCAAAATTTAATCCGCCAACCACGCAGACTGTGCAAG
GCAAAGATGGATTATTTTATATTTATACCTCAGGAACAACTGGATTACCTAAAGCTGTAATTTTTACCCATAGCCGCTGG
ACACTGGCTTATGGTACTTATGGCCACGTACTGGCGCTAGATGAAAATGATGTCATGTACGTGACTCTTCCAATGTATCA
TGCGACAGGTATTGTGGTGTGTTGGTGCGGCGTGATTGCAGGTAGTGCAACACTGGCATTGCGCCGAAAATACTCCACCA
GCGCATTTTGGAAAGATGTACAAAAATTTAATGCATCTGCTATTGGTTATGTGGGTGAACTATGCCGTTATTTAATGGAT
GCACCTCCTTCCGAGCTTGACCGTCATCATCGTGTGACCAAAATGATCGGTAATGGCATGCGCCCCAATATCTGGGATAA
ATTCAAGCAGCGTTTTGGCGTAAAAGAAATTCTTGAACTCTATGCCTCAAGTGAAGGCAACGTCGGGTTTAGCAATATCT
TTAATTTTGACAATACCGTTGGTTTCTCACCTACCCCTTACGCAGTAATTGCCTTTGATAAAGAGAAAAATGAACCTATT
CGTGATCAAAATGGCTACTGCCAGAAAGTGAAAACGGGTGAAGTCGGTTTACTGGTAGGTAAAATCACGCGTCGATCACC
TTTTGATGGATATACCGATCCTGAAAAAAATAAATCGGTTATTCTTAAGAATGTTTTTAAGAAAGGTGATTCATATTTCA
ATACGGGCGATCTGGTGCGTGATATTGGTTTCCGTCATGCTCAATTTGTAGACCGTTTGGGAGATACATTCCGCTGGAAA
GGTGAAAATGTATCAACCACTGAAGTTGAAAATATGCTGTGTGAATACGAGAAGATTGTAGAGGCGGTGGTATACGGCGT
AGAAATTCCAAATACCAACGGACGTGCAGGAATGGCCGCTATTACGCTTAACGATCAGGCCGAATTCAACGATGAAGATA
TGAGCGCGATGGTGAATACCTTTAAAAAATGTTTACCTGCATATGCCATTCCTGTATTTTTACGTGTACAAAAAACGGTC
GAAACGACAGGAACATTTAAATATCAGAAGAATAAACTCAAAGAACAGGCATTTGATCCAAGCAAAACAGAAGAACGTTT
ACTGGTTTTATTACCAGGTGAAAGTGCTTATTGTGAGGTAACTTCAGAGATTTTTGATAACATCCAGGCATATAAGTACC
GTTTTTAG

Upstream 100 bases:

>100_bases
TTGCACTACAACATATCTCGTAAAAGAACAGTTGTAGATTAAACTGCGTATTTTGCTATAGACACGTAAACCTGATTGGA
TTAATCGAAAAATAAATAGG

Downstream 100 bases:

>100_bases
TTCATTTTTTAGCTGAACATGAGGAAATCGTGTTTTTTATAAACAGTCATGCAATTGTTTTGAATTTTCCTCTTGTGTTA
GCTGAATAACTTGCTACAAT

Product: long-chain-acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MHMSQTTQTDIITLGAVAAKIPQLIPKVPHLLNGLKQAYLRTPSTPAGLGIAFEKAVKRNPDGMALLFEDERYSYQELNE
WANQIAHFYLSLGARKGDVIAVMVENRSELLATIVALAKIGVTAALVNTSQTGKVLTHSINLVQPIALIVGEEVRKCVDE
IKQDLNLAQDRFHWFANQATRQDAGSAPRDYINLAEKIDHFPKFNPPTTQTVQGKDGLFYIYTSGTTGLPKAVIFTHSRW
TLAYGTYGHVLALDENDVMYVTLPMYHATGIVVCWCGVIAGSATLALRRKYSTSAFWKDVQKFNASAIGYVGELCRYLMD
APPSELDRHHRVTKMIGNGMRPNIWDKFKQRFGVKEILELYASSEGNVGFSNIFNFDNTVGFSPTPYAVIAFDKEKNEPI
RDQNGYCQKVKTGEVGLLVGKITRRSPFDGYTDPEKNKSVILKNVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWK
GENVSTTEVENMLCEYEKIVEAVVYGVEIPNTNGRAGMAAITLNDQAEFNDEDMSAMVNTFKKCLPAYAIPVFLRVQKTV
ETTGTFKYQKNKLKEQAFDPSKTEERLLVLLPGESAYCEVTSEIFDNIQAYKYRF

Sequences:

>Translated_615_residues
MHMSQTTQTDIITLGAVAAKIPQLIPKVPHLLNGLKQAYLRTPSTPAGLGIAFEKAVKRNPDGMALLFEDERYSYQELNE
WANQIAHFYLSLGARKGDVIAVMVENRSELLATIVALAKIGVTAALVNTSQTGKVLTHSINLVQPIALIVGEEVRKCVDE
IKQDLNLAQDRFHWFANQATRQDAGSAPRDYINLAEKIDHFPKFNPPTTQTVQGKDGLFYIYTSGTTGLPKAVIFTHSRW
TLAYGTYGHVLALDENDVMYVTLPMYHATGIVVCWCGVIAGSATLALRRKYSTSAFWKDVQKFNASAIGYVGELCRYLMD
APPSELDRHHRVTKMIGNGMRPNIWDKFKQRFGVKEILELYASSEGNVGFSNIFNFDNTVGFSPTPYAVIAFDKEKNEPI
RDQNGYCQKVKTGEVGLLVGKITRRSPFDGYTDPEKNKSVILKNVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWK
GENVSTTEVENMLCEYEKIVEAVVYGVEIPNTNGRAGMAAITLNDQAEFNDEDMSAMVNTFKKCLPAYAIPVFLRVQKTV
ETTGTFKYQKNKLKEQAFDPSKTEERLLVLLPGESAYCEVTSEIFDNIQAYKYRF
>Mature_615_residues
MHMSQTTQTDIITLGAVAAKIPQLIPKVPHLLNGLKQAYLRTPSTPAGLGIAFEKAVKRNPDGMALLFEDERYSYQELNE
WANQIAHFYLSLGARKGDVIAVMVENRSELLATIVALAKIGVTAALVNTSQTGKVLTHSINLVQPIALIVGEEVRKCVDE
IKQDLNLAQDRFHWFANQATRQDAGSAPRDYINLAEKIDHFPKFNPPTTQTVQGKDGLFYIYTSGTTGLPKAVIFTHSRW
TLAYGTYGHVLALDENDVMYVTLPMYHATGIVVCWCGVIAGSATLALRRKYSTSAFWKDVQKFNASAIGYVGELCRYLMD
APPSELDRHHRVTKMIGNGMRPNIWDKFKQRFGVKEILELYASSEGNVGFSNIFNFDNTVGFSPTPYAVIAFDKEKNEPI
RDQNGYCQKVKTGEVGLLVGKITRRSPFDGYTDPEKNKSVILKNVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWK
GENVSTTEVENMLCEYEKIVEAVVYGVEIPNTNGRAGMAAITLNDQAEFNDEDMSAMVNTFKKCLPAYAIPVFLRVQKTV
ETTGTFKYQKNKLKEQAFDPSKTEERLLVLLPGESAYCEVTSEIFDNIQAYKYRF

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI227499619, Length=573, Percent_Identity=38.9179755671902, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI40807357, Length=571, Percent_Identity=36.4273204903678, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI62865631, Length=572, Percent_Identity=36.8881118881119, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI13325055, Length=572, Percent_Identity=36.8881118881119, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI38524616, Length=569, Percent_Identity=35.8523725834798, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI13236579, Length=529, Percent_Identity=36.6729678638941, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI13325057, Length=614, Percent_Identity=33.3876221498371, Blast_Score=319, Evalue=5e-87,
Organism=Homo sapiens, GI227499621, Length=338, Percent_Identity=44.9704142011834, Blast_Score=288, Evalue=1e-77,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=26.9598470363289, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17551278, Length=545, Percent_Identity=38.7155963302752, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI115534440, Length=547, Percent_Identity=35.6489945155393, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32563687, Length=545, Percent_Identity=22.2018348623853, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319515, Length=561, Percent_Identity=29.0552584670232, Blast_Score=257, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6319699, Length=387, Percent_Identity=24.031007751938, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=506, Percent_Identity=23.7154150197628, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583465, Length=578, Percent_Identity=37.7162629757785, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24583463, Length=578, Percent_Identity=37.7162629757785, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281364798, Length=578, Percent_Identity=37.7162629757785, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45552801, Length=596, Percent_Identity=37.0805369127517, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45550496, Length=596, Percent_Identity=37.0805369127517, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28573631, Length=541, Percent_Identity=35.6746765249538, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI20130353, Length=541, Percent_Identity=35.6746765249538, Blast_Score=354, Evalue=9e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 68956; Mature: 68956

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMSQTTQTDIITLGAVAAKIPQLIPKVPHLLNGLKQAYLRTPSTPAGLGIAFEKAVKRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
PDGMALLFEDERYSYQELNEWANQIAHFYLSLGARKGDVIAVMVENRSELLATIVALAKI
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
GVTAALVNTSQTGKVLTHSINLVQPIALIVGEEVRKCVDEIKQDLNLAQDRFHWFANQAT
CHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQDAGSAPRDYINLAEKIDHFPKFNPPTTQTVQGKDGLFYIYTSGTTGLPKAVIFTHSRW
HHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEEECCCCCCCCEEEEECCCE
TLAYGTYGHVLALDENDVMYVTLPMYHATGIVVCWCGVIAGSATLALRRKYSTSAFWKDV
EEEECCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHH
QKFNASAIGYVGELCRYLMDAPPSELDRHHRVTKMIGNGMRPNIWDKFKQRFGVKEILEL
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHH
YASSEGNVGFSNIFNFDNTVGFSPTPYAVIAFDKEKNEPIRDQNGYCQKVKTGEVGLLVG
HHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEE
KITRRSPFDGYTDPEKNKSVILKNVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWK
EHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
GENVSTTEVENMLCEYEKIVEAVVYGVEIPNTNGRAGMAAITLNDQAEFNDEDMSAMVNT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
FKKCLPAYAIPVFLRVQKTVETTGTFKYQKNKLKEQAFDPSKTEERLLVLLPGESAYCEV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHH
TSEIFDNIQAYKYRF
HHHHHCCHHHHCCCC
>Mature Secondary Structure
MHMSQTTQTDIITLGAVAAKIPQLIPKVPHLLNGLKQAYLRTPSTPAGLGIAFEKAVKRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
PDGMALLFEDERYSYQELNEWANQIAHFYLSLGARKGDVIAVMVENRSELLATIVALAKI
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
GVTAALVNTSQTGKVLTHSINLVQPIALIVGEEVRKCVDEIKQDLNLAQDRFHWFANQAT
CHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQDAGSAPRDYINLAEKIDHFPKFNPPTTQTVQGKDGLFYIYTSGTTGLPKAVIFTHSRW
HHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEEECCCCCCCCEEEEECCCE
TLAYGTYGHVLALDENDVMYVTLPMYHATGIVVCWCGVIAGSATLALRRKYSTSAFWKDV
EEEECCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHH
QKFNASAIGYVGELCRYLMDAPPSELDRHHRVTKMIGNGMRPNIWDKFKQRFGVKEILEL
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHH
YASSEGNVGFSNIFNFDNTVGFSPTPYAVIAFDKEKNEPIRDQNGYCQKVKTGEVGLLVG
HHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEE
KITRRSPFDGYTDPEKNKSVILKNVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWK
EHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
GENVSTTEVENMLCEYEKIVEAVVYGVEIPNTNGRAGMAAITLNDQAEFNDEDMSAMVNT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
FKKCLPAYAIPVFLRVQKTVETTGTFKYQKNKLKEQAFDPSKTEERLLVLLPGESAYCEV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHH
TSEIFDNIQAYKYRF
HHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA