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The map label for this gene is pnp

Identifier: 50083658

GI number: 50083658

Start: 402324

End: 404417

Strand: Direct

Name: pnp

Synonym: ACIAD0402

Alternate gene names: 50083658

Gene position: 402324-404417 (Clockwise)

Preceding gene: 50083657

Following gene: 50083659

Centisome position: 11.18

GC content: 44.65

Gene sequence:

>2094_bases
ATGTCGATGTTTAATATTATTCGTAAAGAATTTCAGTTTGGTCAACATCAGGTGGTACTAGAAACGGGTCGCGTTGCACG
TCAAGCAAACACCGTTTTAGTCACGATGGGTGGTGTGACGGTATTGGTTGCAGTCGTTGCACAACCTAAAGCGAAAGCGG
GTCAAGACTTTTTCCCACTGACTGTGAACTATCAAGAAAAACAATATGCTGCTGGTCGTATCCCAGGTGGTTATGGCAAG
CGTGAAGGCCGTGCGTCTGAAGCTGAAACTTTAATTTCACGTTTGATTGACCGTCCAATTCGTCCGTTGTTCCCAGAAGG
TTACTTCAACGAAATTCAAATCACCGCGACAGTGGTTTCTTCTGACAAAACGATGGATGCCGATATTGCAGCCATGCTAG
GTGCTTCAGCGGCATTGTCAATTGCGGGTACGCCATTCCGTGGTCCAATCGGTGGTGCACGTGTTGGTTTGATCAATGGC
GAATACGTTTTAAACCCAAATTTTGAGCAACTTGCTCAGTCTGACCTTGACCTTGTCGTTGCGGGTACAGAGTCTGCTGT
ACTCATGGTTGAATCTGAAGCAAAAGAACTTTCAGAAGATCAAATGCTAGGCGCTGTACTTTTTGGTCATGACGAAATGC
AAATTGCAGTTCAAGCGATCAAAGAGTTTGCTGCGGCGGCTGGTGCAGTTGAATCAACTTGGGTTGCGCCAACGAAAAAC
GAAACATTGCTTGAGCAATTAAAGGCAGCGTTTGAAGCTAAAATTTCTGAAGCATATACCATTGCGGTGAAACAAGATCG
TTATACAGCACTTGATGCACTTTATGCAGAAGCTGTAGCTCAGTTCGTTCCAGAAAATGACGAAACGGGTATTGCCGATG
AAGTCAACGAATTATTCGAAGACCTTAAATACCGTACCGTACGTGACAACATCTTGTCAGGTAAGCCACGTATTGATGGT
CGTGATACTAAAACTGTTCGTGCTATCGACGTTCAAGTGGGCGTATTAGATCGTGCACATGGTTCTGCATTATTTACACG
TGGTGAAACTCAGGCGTTGGTGACCACCACTTTGGGTAATACGCGTGATGCGTTAATGGTTGATACCCTTGCGGGTACTA
AAACTGACAACTTCATGTTGCACTACAACTTCCCAGCATACTCAGTGGGTGAAACTGGTCGTGAATCTGGTCCAAAGCGC
CGTGAAATTGGTCATGGTCGTTTGGCACGTCGTGGTGTTCAAGCCGTTTTACCTGCAGCTGACCGCTTCCCGTACGTGAT
TCGTATCGTATCAGACATTACTGAATCAAATGGTTCATCGTCAATGGCGTCTGTATGTGGTGCATCACTTTCATTGATGG
ATGCAGGTGTTCCATTGAAAGCACCAGTTGCGGGTATCGCAATGGGCCTAGTCAAAGAAGGTGAGCGTTTTGCTGTACTT
TCTGACATCTTGGGTGACGAAGATCATTTAGGTGATATGGACTTTAAGGTTGCTGGTTCTGCCAATGGTATTACTGCACT
TCAAATGGACATCAAAATTGAAGGCATCACTGAAGAAATCATGGAAGTTGCATTGAATCAGGCGTATGCAGGTCGTATGC
ACATCCTGAATGAAATGAACAAAGTGATTTCACGTGCGCGTGCTGAAATTTCAGCACATGCACCAACGTTTGAAGTCATT
ACGATCAATCCAGACAAGATTCGTGACGTGATTGGTAAGGGCGGCGCGACGATTCGTCAGATCACTGAAGAAACCAAAGC
CGCAATTGATATTGAAGACAATGGTACAGTGCGTGTATTTGGTGAAACCAAAGCGGCTGCACGTGCCGCAATTGCAAAAA
TTCAGGCCTTAACGGCTGAAGTTGAACCTGGCAAAATCTATGATGGTAAAGTCATCCGTATCGTAGAATTTGGTGCGTTC
GTGAATATCATGCCAGGTACTGATGGTTTGCTACATATTTCACAGATTTCAAATGAACGTGTAGCCAATGTCTCTGATGT
TCTTAAAGAAGGTCAGGATGTTAAAGTACAAGTTGCAGATGTCGACAACCGTGGTCGTATCAAATTGAGTATGAAAGATA
TTGAACAAGCTTAA

Upstream 100 bases:

>100_bases
AGCTTTGTCATTTATCATTGCTGTTGGGGTGATATGAATGCCAAACCTTAGGGGTCTCCACTAGAATAACTGTTCAAAAA
GAACTAGGAAAAAAGATTAC

Downstream 100 bases:

>100_bases
GCCTGTTTCGTAAATAAAAAAGTCCGCTTCGGCGGACTTTTTTAATTTTAGTATTTACAGTCCAGTTCAGTCCAAGGAAT
AGATTAATCACTCAGATTAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MSMFNIIRKEFQFGQHQVVLETGRVARQANTVLVTMGGVTVLVAVVAQPKAKAGQDFFPLTVNYQEKQYAAGRIPGGYGK
REGRASEAETLISRLIDRPIRPLFPEGYFNEIQITATVVSSDKTMDADIAAMLGASAALSIAGTPFRGPIGGARVGLING
EYVLNPNFEQLAQSDLDLVVAGTESAVLMVESEAKELSEDQMLGAVLFGHDEMQIAVQAIKEFAAAAGAVESTWVAPTKN
ETLLEQLKAAFEAKISEAYTIAVKQDRYTALDALYAEAVAQFVPENDETGIADEVNELFEDLKYRTVRDNILSGKPRIDG
RDTKTVRAIDVQVGVLDRAHGSALFTRGETQALVTTTLGNTRDALMVDTLAGTKTDNFMLHYNFPAYSVGETGRESGPKR
REIGHGRLARRGVQAVLPAADRFPYVIRIVSDITESNGSSSMASVCGASLSLMDAGVPLKAPVAGIAMGLVKEGERFAVL
SDILGDEDHLGDMDFKVAGSANGITALQMDIKIEGITEEIMEVALNQAYAGRMHILNEMNKVISRARAEISAHAPTFEVI
TINPDKIRDVIGKGGATIRQITEETKAAIDIEDNGTVRVFGETKAAARAAIAKIQALTAEVEPGKIYDGKVIRIVEFGAF
VNIMPGTDGLLHISQISNERVANVSDVLKEGQDVKVQVADVDNRGRIKLSMKDIEQA

Sequences:

>Translated_697_residues
MSMFNIIRKEFQFGQHQVVLETGRVARQANTVLVTMGGVTVLVAVVAQPKAKAGQDFFPLTVNYQEKQYAAGRIPGGYGK
REGRASEAETLISRLIDRPIRPLFPEGYFNEIQITATVVSSDKTMDADIAAMLGASAALSIAGTPFRGPIGGARVGLING
EYVLNPNFEQLAQSDLDLVVAGTESAVLMVESEAKELSEDQMLGAVLFGHDEMQIAVQAIKEFAAAAGAVESTWVAPTKN
ETLLEQLKAAFEAKISEAYTIAVKQDRYTALDALYAEAVAQFVPENDETGIADEVNELFEDLKYRTVRDNILSGKPRIDG
RDTKTVRAIDVQVGVLDRAHGSALFTRGETQALVTTTLGNTRDALMVDTLAGTKTDNFMLHYNFPAYSVGETGRESGPKR
REIGHGRLARRGVQAVLPAADRFPYVIRIVSDITESNGSSSMASVCGASLSLMDAGVPLKAPVAGIAMGLVKEGERFAVL
SDILGDEDHLGDMDFKVAGSANGITALQMDIKIEGITEEIMEVALNQAYAGRMHILNEMNKVISRARAEISAHAPTFEVI
TINPDKIRDVIGKGGATIRQITEETKAAIDIEDNGTVRVFGETKAAARAAIAKIQALTAEVEPGKIYDGKVIRIVEFGAF
VNIMPGTDGLLHISQISNERVANVSDVLKEGQDVKVQVADVDNRGRIKLSMKDIEQA
>Mature_696_residues
SMFNIIRKEFQFGQHQVVLETGRVARQANTVLVTMGGVTVLVAVVAQPKAKAGQDFFPLTVNYQEKQYAAGRIPGGYGKR
EGRASEAETLISRLIDRPIRPLFPEGYFNEIQITATVVSSDKTMDADIAAMLGASAALSIAGTPFRGPIGGARVGLINGE
YVLNPNFEQLAQSDLDLVVAGTESAVLMVESEAKELSEDQMLGAVLFGHDEMQIAVQAIKEFAAAAGAVESTWVAPTKNE
TLLEQLKAAFEAKISEAYTIAVKQDRYTALDALYAEAVAQFVPENDETGIADEVNELFEDLKYRTVRDNILSGKPRIDGR
DTKTVRAIDVQVGVLDRAHGSALFTRGETQALVTTTLGNTRDALMVDTLAGTKTDNFMLHYNFPAYSVGETGRESGPKRR
EIGHGRLARRGVQAVLPAADRFPYVIRIVSDITESNGSSSMASVCGASLSLMDAGVPLKAPVAGIAMGLVKEGERFAVLS
DILGDEDHLGDMDFKVAGSANGITALQMDIKIEGITEEIMEVALNQAYAGRMHILNEMNKVISRARAEISAHAPTFEVIT
INPDKIRDVIGKGGATIRQITEETKAAIDIEDNGTVRVFGETKAAARAAIAKIQALTAEVEPGKIYDGKVIRIVEFGAFV
NIMPGTDGLLHISQISNERVANVSDVLKEGQDVKVQVADVDNRGRIKLSMKDIEQA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=717, Percent_Identity=36.9595536959554, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=61.4161849710983, Blast_Score=861, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=78, Percent_Identity=46.1538461538462, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=710, Percent_Identity=32.8169014084507, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17535281, Length=78, Percent_Identity=53.8461538461538, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320850, Length=105, Percent_Identity=37.1428571428571, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281362905, Length=708, Percent_Identity=36.4406779661017, Blast_Score=414, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=708, Percent_Identity=36.4406779661017, Blast_Score=414, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=708, Percent_Identity=36.4406779661017, Blast_Score=414, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=662, Percent_Identity=35.6495468277946, Blast_Score=377, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_ACIAD (Q6FF12)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045168.1
- HSSP:   P05055
- ProteinModelPortal:   Q6FF12
- SMR:   Q6FF12
- STRING:   Q6FF12
- GeneID:   2879799
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0402
- NMPDR:   fig|62977.3.peg.953
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q6FF12
- ProtClustDB:   PRK11824
- BioCyc:   ASP62977:ACIAD0402-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75058; Mature: 74927

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFNIIRKEFQFGQHQVVLETGRVARQANTVLVTMGGVTVLVAVVAQPKAKAGQDFFPL
CCHHHHHHHHHHCCCEEEEEECCHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCEEEE
TVNYQEKQYAAGRIPGGYGKREGRASEAETLISRLIDRPIRPLFPEGYFNEIQITATVVS
EEECCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEE
SDKTMDADIAAMLGASAALSIAGTPFRGPIGGARVGLINGEYVLNPNFEQLAQSDLDLVV
CCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHCCCCCEEE
AGTESAVLMVESEAKELSEDQMLGAVLFGHDEMQIAVQAIKEFAAAAGAVESTWVAPTKN
ECCCCEEEEEECCHHHCCHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ETLLEQLKAAFEAKISEAYTIAVKQDRYTALDALYAEAVAQFVPENDETGIADEVNELFE
HHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DLKYRTVRDNILSGKPRIDGRDTKTVRAIDVQVGVLDRAHGSALFTRGETQALVTTTLGN
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECCCCCEEEEEECCC
TRDALMVDTLAGTKTDNFMLHYNFPAYSVGETGRESGPKRREIGHGRLARRGVQAVLPAA
CCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCC
DRFPYVIRIVSDITESNGSSSMASVCGASLSLMDAGVPLKAPVAGIAMGLVKEGERFAVL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGSANGITALQMDIKIEGITEEIMEVALNQAYAGRMHILNEMN
HHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
KVISRARAEISAHAPTFEVITINPDKIRDVIGKGGATIRQITEETKAAIDIEDNGTVRVF
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCEEEEE
GETKAAARAAIAKIQALTAEVEPGKIYDGKVIRIVEFGAFVNIMPGTDGLLHISQISNER
ECCHHHHHHHHHHHHHHHEECCCCEEECCCEEEEEEECCEEEEECCCCCEEEEEHHCCCH
VANVSDVLKEGQDVKVQVADVDNRGRIKLSMKDIEQA
HCCHHHHHHCCCCEEEEEEECCCCCEEEEEHHCCCCC
>Mature Secondary Structure 
SMFNIIRKEFQFGQHQVVLETGRVARQANTVLVTMGGVTVLVAVVAQPKAKAGQDFFPL
CHHHHHHHHHHCCCEEEEEECCHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCEEEE
TVNYQEKQYAAGRIPGGYGKREGRASEAETLISRLIDRPIRPLFPEGYFNEIQITATVVS
EEECCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEE
SDKTMDADIAAMLGASAALSIAGTPFRGPIGGARVGLINGEYVLNPNFEQLAQSDLDLVV
CCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHCCCCCEEE
AGTESAVLMVESEAKELSEDQMLGAVLFGHDEMQIAVQAIKEFAAAAGAVESTWVAPTKN
ECCCCEEEEEECCHHHCCHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ETLLEQLKAAFEAKISEAYTIAVKQDRYTALDALYAEAVAQFVPENDETGIADEVNELFE
HHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DLKYRTVRDNILSGKPRIDGRDTKTVRAIDVQVGVLDRAHGSALFTRGETQALVTTTLGN
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECCCCCEEEEEECCC
TRDALMVDTLAGTKTDNFMLHYNFPAYSVGETGRESGPKRREIGHGRLARRGVQAVLPAA
CCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCC
DRFPYVIRIVSDITESNGSSSMASVCGASLSLMDAGVPLKAPVAGIAMGLVKEGERFAVL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGSANGITALQMDIKIEGITEEIMEVALNQAYAGRMHILNEMN
HHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
KVISRARAEISAHAPTFEVITINPDKIRDVIGKGGATIRQITEETKAAIDIEDNGTVRVF
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCEEEEE
GETKAAARAAIAKIQALTAEVEPGKIYDGKVIRIVEFGAFVNIMPGTDGLLHISQISNER
ECCHHHHHHHHHHHHHHHEECCCCEEECCCEEEEEEECCEEEEECCCCCEEEEEHHCCCH
VANVSDVLKEGQDVKVQVADVDNRGRIKLSMKDIEQA
HCCHHHHHHCCCCEEEEEEECCCCCEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA